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Aminoglycosides And Syringomycin E As Fungicides Against Fusarium Graminearum In Head Blight Disease, Yukie Kawasaki

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Fusarium graminearum is one of the most problematic phytopathogens in US agriculture. This fungus causes head blight, foot rot, and damping off on wheat and barley. The infection lowers the grain yield and causes contamination of the grain product with mycotoxins. Effective control measures are lacking, and new fungicides that kill F. graminearum but remain safe and economical to use are needed. Newly synthesized aminoglycosides (JL22, JL38, JL39, JL40, NEOF004, NEOF005), classic aminoglycosides (amikacin, gentamicin, kanamycin A, kanamycin B, neomycin, and ribostamycin), and a lipopeptide, syringomycin E (SRE), were studied to determine their antifungal potential to control F. graminearum. …

Understanding Polyamine Metabolism Through Transgenic Manipulation In Poplar Suspension Cultures, Sridev Mohapatra

Doctoral Dissertations

Polyamines are low molecular weight aliphatic amines that are obligatory requirements for cell survival and growth. The commonly occurring polyamines in plants are putrescine, spermidine, and spermine. Suspension cultures of poplar (Populus nigra x maximowiczii), transformed with a mouse ornithine decarboxylase gene (under the control of a 2X 35S CaMV promoter) were used to study the impact of up-regulation of putrescine biosynthesis (and consequent enhanced catabolism) on several aspects of cellular metabolism. The transgenic cells were compared with a control cell line that was transformed with the beta-glucuronidase (GUS) gene.

It was observed that enhanced putrescine metabolism resulted in: (i) …

Identification And Characterization Of Protein Phosphatase 2a Mutants In Arabidopsis Thaliana, Enhua Wang

Doctoral Dissertations

Reversible protein phosphorylation is a highly regulated process that affects almost all cellular activities. Phosphorylation involves two groups of enzymes: protein kinases and protein phosphatases. Considering the central role of these enzymes in cells, elucidating their function is extremely important. My research focuses on protein phosphatase 2A (PP2A) C subunits. PP2A is one of the most abundant types of serine/threonine phosphatases in all eukaryotic cells. Compared with animals, PP2A function is not well known in plants. Early studies using protein phosphatase inhibitors are not useful to demonstrate the function of individual PP2A subunits. A reverse genetic approach can be helpful …

Survival Strategies Of Eelgrass In Reduced Light, Caroline A. Ochieng

Doctoral Dissertations

Light reduction due to anthropogenic impacts is the most widespread cause of worldwide decline of eelgrass, an ecologically important marine angiosperm whose role in supporting overall coastal ecosystem productivity has been widely recognized. Understanding eelgrass plant and meadow responses to light reduction has therefore received significant research interest over the last 30 years, while managers have sought tools to identify critical thresholds for light availability and predict impacts of human-induced disturbances in order to prevent further eelgrass loss. In the present thesis, a review of some of the literature on light reduction and its effects on eelgrass (i) summarized the …

Spectral Effects Of A Calcium Amendment On Red Spruce Foliage At Laboratory And Stand Scale, Will Robinson Kessler

Master's Theses and Capstones

Three sets of measurements were made to determine the effects of an October 1999 whole-watershed Ca-application on the chemical and spectral properties of red spruce (Picea rubens Sarg.) foliage at the Hubbard Brook Experimental Forest. Results of our measurements showed significant differences between the Ca-treated watershed (WS1) and a nearby reference watershed (WS6). Foliar chemistry data collected in 2007 showed that concentrations of Ca, Sr, and oxalate remain higher in WS1, and the increase in oxalate is strongly linked to the level of total Ca, possibly by a Ca-oxalate crystal precipitation response. High-resolution laboratory spectral data measured from 400-2500 nm …