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Genetic Engineering Of The Polyamine Biosynthetic Pathway And Somatic Embryogenesis In Carrot (Daucus Carota L), Dhundy Raj Bastola
Genetic Engineering Of The Polyamine Biosynthetic Pathway And Somatic Embryogenesis In Carrot (Daucus Carota L), Dhundy Raj Bastola
Doctoral Dissertations
Ornithine decarboxylase (ODC), arginine decarboxylase (ADC), and S-adenosylmethionine decarboxylase (SAMDC) are three of the key regulatory enzymes involved in the biosynthesis of polyamines (putrescine, spermidine and spermine). To modulate the biosynthesis of putrescine, carrot (Daucus carota L.) cells were transformed with Agrobacterium tumefaciens strains containing 3$\sp\prime$-truncated mouse ornithine decarboxylase (ODC) cDNA under the control of a CaMV 35S promoter. Neomycin phosphotransferase gene linked with nopaline synthase promoter was used to select transformed cell lines on kanamycin. While the non-transformed cells contained no ODC, high levels of ODC activity were observed in the transformed cells. A significant increase in the cellular …
Steady-State Natural Fluorescence, Arnold Francis Theisen
Steady-State Natural Fluorescence, Arnold Francis Theisen
Doctoral Dissertations
This study employed a manifold analytical approach to the application of multiple excitation and emission wavelengths of steady-state natural fluorescence for the detection of stressed and damaged vegetation. This investigator has attempted to answer questions put forth in terms of the following null hypothesis: Natural fluorescence emission, measured in the steady-state from living, attached or freshly excised needles of eastern white pine (Pinus strobus L.) or leaves of common beans (Phaseolus vulgaris L.), cannot be used in the form of complete spectra or in ratios of discrete wavelengths as indicators of stress from abiotic sources such as ozone or cadmium. …
Ecological Aspects Of Iron Acquisition In Synechococcus Sp (Cyanophyceae), Steven Wray Wilhelm
Ecological Aspects Of Iron Acquisition In Synechococcus Sp (Cyanophyceae), Steven Wray Wilhelm
Digitized Theses
As primary producers, marine cyanobacteria regulate the biological and biogeochemical cycles of aquatic ecosystems and influence ocean-atmosphere gas exchange. The purposes of this project were to determine if a Synechococcus sp. was capable of utilizing a high-affinity iron transport system during periods of iron-limited growth and if physiological changes due to iron limitation could be identified. Resolution of these factors was achieved by maintaining the cyanobacterium Synechococcus sp. PCC 7002 in continuous culture chemostats over a range of iron availabilities.;Changes in physiology were detected over a range of iron concentrations. Reductions in the levels of photosynthetic pigments were accompanied by …