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Expression Of The Murine Ornithine Decarboxylase Gene In Transgenic Nicotiana Tabacum Var Xanthi, Richard Anthony Descenzo
Expression Of The Murine Ornithine Decarboxylase Gene In Transgenic Nicotiana Tabacum Var Xanthi, Richard Anthony Descenzo
Doctoral Dissertations
Ornithine decarboxylase (ODC), arginine decarboxylase (ADC), and S-adenosyl-methionine decarboxylase (SAMDC) are the three key regulatory enzymes for polyamine (putrescine, spermidine, and spermine) biosynthesis. In order to gain more insight into the relationship between polyamine metabolism and other physiological processes, research was undertaken to obtain increased putrescine biosynthesis in tobacco by overexpression of a murine ODC cDNA.
Both a full-length and a truncated murine ODC cDNA were cloned into a binary expression vector containing the Cauliflower Mosaic Virus (CaMV) 35S promoter. Using standard leaf-disc transformation procedures, transgenic tobacco plants containing either the full length or the truncated ODC cDNA were obtained. …
Genetic, Physiological And Anatomical Analyses Of A Wilty Mutant In Tomato (Lycopersicon Esculentum Mill, Cv Large Plum), Karen Elizabeth Rasmussen
Genetic, Physiological And Anatomical Analyses Of A Wilty Mutant In Tomato (Lycopersicon Esculentum Mill, Cv Large Plum), Karen Elizabeth Rasmussen
Doctoral Dissertations
Large Plum', a tomato cultivar carrying a wilty mutation (LP-wilty), was characterized genetically, physiologically, and anatomically. Inheritance, complementation, and mapping studies of LP-wilty were performed. The mutation was found to be inherited as a single dominant gene, distinct from other wilty alleles, and was mapped to the long arm of chromosome six.
Measurements were taken of stomatal conductance, root conductance, shoot water potential, and leaf water potential for 'Large Plum' and normal plants. Results suggest that both stomatal conductance and root conductance rates are reduced in the wilty mutants. In addition, shoot and leaf water potentials are more negative in …