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Evaluation Of The Virulence Potential Of Avian Pathogenic Escherichia Coli Isolated From Broiler Breeders With Colibacillosis In Mississippi, Jiddu Joseph Aug 2023

Evaluation Of The Virulence Potential Of Avian Pathogenic Escherichia Coli Isolated From Broiler Breeders With Colibacillosis In Mississippi, Jiddu Joseph

Theses and Dissertations

Avian pathogenic Escherichia coli (APEC) is a bacterium that is responsible for colibacillosis in birds. However, information about broiler breeder APEC isolates is limited, but the data is critical due to the transfer of this bacteria down the production pyramid to progenies resulting in high mortality. Therefore, we evaluated the phenotypic virulence characteristics of 28 isolates using embryo lethality and day-old chick challenge assays. Also, the in vitro adhesion and invasion potential of selected nine isolates were identified. Results showed more than 1/3rd of the isolates were highly virulent and the virulence increased as the number of virulence-associated genes …


Prediction Of Chicken Embryo Lethality With The Avian Escherichia Coli Traits Complement Resistance, Colicin V Production, And Presence Of The Increased Serum Survival Gene Cluster (Iss), Penelope S. Gibbs, John J. Maurer, Lisa K. Nolan, Richard E. Wooley Apr 2003

Prediction Of Chicken Embryo Lethality With The Avian Escherichia Coli Traits Complement Resistance, Colicin V Production, And Presence Of The Increased Serum Survival Gene Cluster (Iss), Penelope S. Gibbs, John J. Maurer, Lisa K. Nolan, Richard E. Wooley

Lisa K. Nolan

Differentiating between virulent and avirulent avian Escherichia coli isolates continues to be a problem for poultry diagnostic laboratories and the study of colibacillosis in poultry. The ability of a laboratory to conduct one simple test that correlates with virulence would simplify studies in these areas; however, previous studies have not enabled researchers to establish such a test. In this study, the occurrence of certain phenotypic and genotypic traits purported to contribute to avian E. coli virulence in 20 avian E. coli isolates was correlated with the results of embryo challenge studies. This analysis was undertaken in an effort to determine …