Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 5 of 5
Full-Text Articles in Entire DC Network
The Influence Of Fluorescent Light On The Development Of In Vitro Fertilized Bovine Oocytes, Jared Bunch
The Influence Of Fluorescent Light On The Development Of In Vitro Fertilized Bovine Oocytes, Jared Bunch
Undergraduate Honors Capstone Projects
Advances in in vitro fertilization and in vitro culture techniques have allowed considerable progress in identifying physiological requirements of mammalian embryos. Parrish et al. (11) reported a major breakthrough on in vitro fertilization when his group identified heparin as an important factor for the capacitation of spermatozoa. Capacitation is necessary for fertilization of matured oocytes. During the precoculture era of embryos (prior to 1980), the development of early preirnplantation embryos was vary limited regardless of medium, medium supplement, gas atmosphere, osmolarity or pH used (7). Coculture techniques of fertilized oocytes using somatic cells during in vitro production, particularly of bovine …
The Effect Of Thyroid Hormone On The Expression Of A7 Myosin Heavy Chain Mrna In Xenopus Laevis, Bradley Thomas Butkovich
The Effect Of Thyroid Hormone On The Expression Of A7 Myosin Heavy Chain Mrna In Xenopus Laevis, Bradley Thomas Butkovich
Master's Theses
It has long been known that thyroid hormone (TH) is responsible for metamorphosis in tadpoles. It is also known that A7 MHC, an adult muscle isoform, is expressed in adult Xenopus laevis frogs, but not in larvae. Furthermore, the appearance of A7 corresponds with the onset of metamorphosis. The present study investigated the relationship between TH and the expression of A7 MHC mRNA in Xenopus laevis. Isolated tails in culture and 6-n-propyl 2-thiouracil (PTU) treated tails were treated with TH and the presence of A7 mRNA analyzed with in-situ hybridization, and RT-PCR. It was found that TH caused substantial …
Development Of An Ovine Genome Map With Emphasis On In Situ Hybridization, Melanie R. Heaton
Development Of An Ovine Genome Map With Emphasis On In Situ Hybridization, Melanie R. Heaton
All Graduate Theses and Dissertations, Spring 1920 to Summer 2023
Livestock genome maps are used to identify economic trait loci and loci proximal to genes affecting economically important traits. This research contributes to the development of the ovine genome map by establishing techniques to physically map large DNA inserts to ovine chromosomes using fluorescent in situ hybridization. Fluorescent in situ hybridization techniques were established using yeast artificial chromosomes as DNA probes. Probes were as large as 1,000,000 kilobases and hybridized to fixed ovine metaphase chromosomes from a callipyge ewe. Three yeast artificial chromosome probes contained a genetic marker for the ovine callipyge gene. Probes were assigned to ovine chromosome pair …
Development Of An Enzyme Linked Immunosorbent Assay Capable Of Detecting Antibodies Specific To The Cytomegalovirus, Rachel Bird
Development Of An Enzyme Linked Immunosorbent Assay Capable Of Detecting Antibodies Specific To The Cytomegalovirus, Rachel Bird
Undergraduate Honors Capstone Projects
The cytomegalovirus (CMV) is capable of causing serious illness and death in immunocompromised individuals. The objective of this research is to develop a method of detecting antibodies against CMV. This has lead to the development of an Enzyme linked Immunosorbent Assay (ELISA) that is capable of detecting CMV or anti-CMV antibodies. The process in the development of such an assay involves; 1) the purification of Hyperimmune Rabbit Serum containing anti-CMV antibodies, 2) determination of the appropriate concentrations necessary to provide a clear and concise response to the test, and 3) testing of monoclonal antibodies to determine their specificity towards the …
The Effects Of Thyroid Hormone On Myosin Heavy Chain A-7 Production In Xenopus Laevis Tail And Leg Muscles, Andrew R. Noble
The Effects Of Thyroid Hormone On Myosin Heavy Chain A-7 Production In Xenopus Laevis Tail And Leg Muscles, Andrew R. Noble
Honors Theses
The present research examines the expression of myosin heavy chain genes in muscle fibers in Xenopus laevis. Characteristically different muscle fibers are expressed before and after metamorphosis. The embryonic myosin proteins that are synthesized during the tadpole stages are replaced with adult myosin heavy chains at metamorphosis. At least one of the adult myosin genes, called A-7, is expressed only in the adult animals, not in tadpoles. The controlling factor or trigger for the expression of this adult gene still remains unknown. There are a number of possible explanations for the A-7 regulation at metamorphosis, including changes in particular hormone …