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Development Of An Ovine Genome Map With Emphasis On In Situ Hybridization, Melanie R. Heaton
Development Of An Ovine Genome Map With Emphasis On In Situ Hybridization, Melanie R. Heaton
All Graduate Theses and Dissertations, Spring 1920 to Summer 2023
Livestock genome maps are used to identify economic trait loci and loci proximal to genes affecting economically important traits. This research contributes to the development of the ovine genome map by establishing techniques to physically map large DNA inserts to ovine chromosomes using fluorescent in situ hybridization. Fluorescent in situ hybridization techniques were established using yeast artificial chromosomes as DNA probes. Probes were as large as 1,000,000 kilobases and hybridized to fixed ovine metaphase chromosomes from a callipyge ewe. Three yeast artificial chromosome probes contained a genetic marker for the ovine callipyge gene. Probes were assigned to ovine chromosome pair …