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Analysis Of Drosophila Insulator Protein Function In Replication Timing And The Osmotic Stress Response, Emily Stow
Analysis Of Drosophila Insulator Protein Function In Replication Timing And The Osmotic Stress Response, Emily Stow
Doctoral Dissertations
Chromatin insulators contribute to the three-dimensional organization of the eukaryotic genome. Insulators and their associated proteins form boundaries between differing chromatin environments, regulate enhancer-promoter interactions, and contribute to the formation of distal genomic contacts. Growing evidence suggests chromatin insulators play roles in cellular processes that extend beyond genome organization alone. The collection of work presented here investigates the involvement of insulators in the timing of DNA replication, the nuclear response to osmotic stress, and the maintenance of genome stability. Chapter 1 characterizes a novel component of Su(Hw) insulator protein complex in Drosophila melanogaster and its contributions to insulator function, genome …
Proteomic And Genomic Evolution Of Phicbkviruses, Kiesha Wilson
Proteomic And Genomic Evolution Of Phicbkviruses, Kiesha Wilson
Theses and Dissertations
It is estimated that there are 1031 phages in the global population. They continue to maintain their populations by undergoing 1024 infections per second. Every time these phages replicate, there is potential for genetic exchange and mutations. Because of this potential, it has been assumed that phage evolution is heavily influenced by mosaicism. However, it is now clear that phage evolution varies by host type and phage type. Here, we use genomic and proteomic analyses to study the evolution of phages that infect Caulobacter crescentus. The study began by comparing six bacteriophage genomes that were larger than …
Novel Insights Into The Multifaceted Roles Of Blm In The Maintenance Of Genome Stability, Vivek M. Shastri
Novel Insights Into The Multifaceted Roles Of Blm In The Maintenance Of Genome Stability, Vivek M. Shastri
USF Tampa Graduate Theses and Dissertations
Genomic instability is a hallmark of disorders in which DNA replication and repair genes are dysfunctional. The tumor suppressor RECQ helicase gene BLM encodes the 3’-5’ DNA Bloom syndrome helicase BLM, which plays important roles during DNA replication, recombination and repair to maintain genome stability. Mutations within BLM cause Bloom syndrome, an autosomal recessive disorder characterized by growth defects, immunodeficiency, >10-fold higher sister chromatid exchange compared to normal cells, and an increased predisposition to a wide range of cancers from an early age. Single nucleotide polymorphisms or SNPs in BLM have been reported to be associated with susceptibility to a …
Interactions Between The Organellar Pol1a, Pol1b, And Twinkle Dna Replication Proteins And Their Role In Plant Organelle Dna Replication, Stewart Anthony Morley
Interactions Between The Organellar Pol1a, Pol1b, And Twinkle Dna Replication Proteins And Their Role In Plant Organelle Dna Replication, Stewart Anthony Morley
Theses and Dissertations
Plants maintain organelle genomes that are descended from ancient microbes. Ages ago, these ancient microbes were engulfed by larger cells, beginning a process of co-evolution we now call the endo-symbiotic theory. Over time, DNA from the engulfed microbe was transferred to the genome of the larger engulfing cell, eventually losing the ability to be free-living, and establishing a permanent residency in the larger cell. Similarly, the larger cell came to rely so much on the microbe it had engulfed, that it too lost its ability to survive without it. Thus, mitochondria and plastids were born. Nearly all multicellular eukaryotes possess …
The Vaccinia Virus (Vacv) B1 And Cellular Vrk2 Kinases Promote Vacv Replication Factory Formation Through Phosphorylation-Dependent Inhibition Of Vacv B12, Annabel T. Olson, Zhigang Wang, Annabel Olson, Alexandria C. Linville, Brianna L. Bullard, Eric A. Weaver, Clinton Jones, Matthew S. Wiebe
The Vaccinia Virus (Vacv) B1 And Cellular Vrk2 Kinases Promote Vacv Replication Factory Formation Through Phosphorylation-Dependent Inhibition Of Vacv B12, Annabel T. Olson, Zhigang Wang, Annabel Olson, Alexandria C. Linville, Brianna L. Bullard, Eric A. Weaver, Clinton Jones, Matthew S. Wiebe
Nebraska Center for Virology: Faculty Publications
Comparative examination of viral and host protein homologs reveals novel mechanisms governing downstream signaling effectors of both cellular and vi- ral origin. The vaccinia virus B1 protein kinase is involved in promoting multiple facets of the virus life cycle and is a homolog of three conserved cellular enzymes called vaccinia virus-related kinases (VRKs). Recent evidence indicates that B1 and VRK2 mediate a com- mon pathway that is largely uncharacterized but appears independent of previous VRK substrates. Interestingly, separate studies described a novel role for B1 in inhibiting vac- cinia virus protein B12, which otherwise impedes an early event in the …
Functional Analysis Of The Replication Fork Proteome Identifies Bet Proteins As Pcna Regulators, Sarah R. Wessel, Kareem N. Mohni, Jessica W. Luzwick, Huzefa Dungrawala, David Cortez
Functional Analysis Of The Replication Fork Proteome Identifies Bet Proteins As Pcna Regulators, Sarah R. Wessel, Kareem N. Mohni, Jessica W. Luzwick, Huzefa Dungrawala, David Cortez
Molecular Biosciences Faculty Publications
Identifying proteins that function at replication forks is essential to understanding DNA replication, chromatin assembly, and replication-coupled DNA repair mechanisms. Combining quantitative mass spectrometry in multiple cell types with stringent statistical cutoffs, we generated a high-confidence catalog of 593 proteins that are enriched at replication forks and nascent chromatin. Loss-of-function genetic analyses indicate that 85% yield phenotypes that are consistent with activities in DNA and chromatin replication or already have described functions in these processes. We illustrate the value of this resource by identifying activities of the BET family proteins BRD2, BRD3, and BRD4 in controlling DNA replication. These proteins …
Dynamics Of The Bacterial Replisome: Biochemical And Single-Molecule Studies Of The Replicative Helicase In Escherichia Coli, Enrico Monachino
Dynamics Of The Bacterial Replisome: Biochemical And Single-Molecule Studies Of The Replicative Helicase In Escherichia Coli, Enrico Monachino
University of Wollongong Thesis Collection 2017+
The replisome is a complex, multi-protein machinery that copies genomic DNA before cell division and allows the faithful transmission of genetic information to the next generation. Error-free duplication is achieved through a fine-tuned coordination among the various components of the replisome. Biochemical and biophysical techniques have contributed tremendously to exposing the role of each component and interactions within the replisome. The recent introduction of single-molecule approaches has revolutionized our understanding of complex protein systems by allowing access to molecular dynamics without the need for population averaging. Advances in improving the throughput of single-molecule techniques allow researchers to reliably sample subpopulations …