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Screening The Cultivable Cave Microbial Mats For The Production Of Antimicrobial Compounds And Antibiotic Resistance, Jerneja Ambrožič Avguštin, Patricia Petrič, Lejla Pašić Dec 2019

Screening The Cultivable Cave Microbial Mats For The Production Of Antimicrobial Compounds And Antibiotic Resistance, Jerneja Ambrožič Avguštin, Patricia Petrič, Lejla Pašić

International Journal of Speleology

The current work extends the phenotypic characterization of a bacterial culture collection obtained from white, yellow, grey or pink microbial cave wall colonies that are common in the caves of Slovenian Karst. We have determined antibiotic resistance to 22 natural and synthetic antibiotics in 69 isolates from the microbial mats. Thirty-eight isolates (52%) were resistant to 1-5 antibiotics; another 27 isolates (37%) were resistant to 6-10 antibiotics; and 7 isolates (0.1%) were resistant to 11-17 antibiotics. We screened for production of antimicrobial compounds by growing cave isolates on five different media and overlaying individual cultures with ten Gram-positive and Gram …


J. Michael Queen (1948 – 2019): A Memorial, Arthur N. Palmer Nov 2019

J. Michael Queen (1948 – 2019): A Memorial, Arthur N. Palmer

International Journal of Speleology

No abstract provided.


Drivers Of Ant Composition, Richness, And Trophic Guilds In Neotropical Iron Ore Cavities, Rodrigo A. Castro-Souza, Thais G. Pellegrini, Marconi Souza-Silva, Rodrigo L. Ferreira Nov 2019

Drivers Of Ant Composition, Richness, And Trophic Guilds In Neotropical Iron Ore Cavities, Rodrigo A. Castro-Souza, Thais G. Pellegrini, Marconi Souza-Silva, Rodrigo L. Ferreira

International Journal of Speleology

Subterranean habitats may be considered limiting for animal colonization, especially for ants, due to permanent darkness and mainly because of oligotrophic conditions. While not as deep as limestone caves, iron ore caves and other subterranean habitats may be more available for colonization because of their shallower depth. We use the richness and composition of ants to assess how differences in habitat structure affect the biodiversity and ecosystem function between cavities and surrounding epigean landscapes. We predicted that the distribution of ants would be different because of the variation in habitat structure and cavity conditions may act as a filter for …


Marcian D. Bleahu (1924-2019), Bogdan P. Onac Nov 2019

Marcian D. Bleahu (1924-2019), Bogdan P. Onac

International Journal of Speleology

No abstract provided.


Mattes J., 2019. Wissenskulturen Des Subterranen. Vermittler Im Spannungsfeld Zwischen Wissenschaft Und Öffentlichkeit. Ein Biographisches Lexikon. [The Culture Of Subterranean Knowledge. Mediators In The Field Of Tension Between Science And Public. A Biographical Lexicon], Monika Schöner Oct 2019

Mattes J., 2019. Wissenskulturen Des Subterranen. Vermittler Im Spannungsfeld Zwischen Wissenschaft Und Öffentlichkeit. Ein Biographisches Lexikon. [The Culture Of Subterranean Knowledge. Mediators In The Field Of Tension Between Science And Public. A Biographical Lexicon], Monika Schöner

International Journal of Speleology

No abstract provided.


The Lampenflora In Show Caves And Its Treatment: An Emerging Ecological Problem, Carlos Baquedano Estévez, Luis Moreno Merino, Almudena De La Losa Román, Juan J. Durán Valsero Oct 2019

The Lampenflora In Show Caves And Its Treatment: An Emerging Ecological Problem, Carlos Baquedano Estévez, Luis Moreno Merino, Almudena De La Losa Román, Juan J. Durán Valsero

International Journal of Speleology

The artificial lighting of caves adapted for touristic visits, leads to the appearance and propagation of a complex community of phototrophic organisms known as “lampenflora”. Formed mainly by algae and cyanobacteria, they produce the degradation of the colonized substrates and decrease the show value of the caves. This phenomenon became famous worldwide in the 1960s due to the damage caused to the paintings in the Lascaux Cave (France). Since then it has become an issue of serious concern to both managers of show caves and to the international scientific community. Over time, the problem has been approached following two complementary …


Barite Replacement Boxwork In The Frasassi Caves (Italy), Sandro Galdenzi Sep 2019

Barite Replacement Boxwork In The Frasassi Caves (Italy), Sandro Galdenzi

International Journal of Speleology

The Frasassi caves, located in the Sentino River Gorge in Ancona Province (Marche, Italy), contains boxwork in a small zone in the inner part of the cave system where it is closely associated with subaqueous corrosion produced by sulfuric acid speleogenesis. The boxwork consists of barite that replaces calcite spar-filled veins and limestone porosity and projects from the corroded cave walls. The replacement involved only the calcite that protrudes from the rock surfaces, indicating that the process took place in the cave environment, and therefore the boxwork is not simply a product of differential corrosion. I hypothesize that the boxwork …


Petrography And Provenance Of Floor Sediments From The Loutra Almopias Cave (Pella, Macedonia, Greece), Ioannis Georgiadis, Katerina Chatzopoulou, Nikolaos Kantiranis, Ioakeim Ioakeimidis, Ananias Tsirambides Sep 2019

Petrography And Provenance Of Floor Sediments From The Loutra Almopias Cave (Pella, Macedonia, Greece), Ioannis Georgiadis, Katerina Chatzopoulou, Nikolaos Kantiranis, Ioakeim Ioakeimidis, Ananias Tsirambides

International Journal of Speleology

Thirty seven floor sediment samples of Upper Pleistocene age from the Loutra Almopias Cave were collected from different beds and stratigraphic columns on the basis of their induration grade, grain distribution, and paleontological findings. Channel facies make up the bulk of the clastic sediments found in the cave passages. Slackwater facies compose the final layer of all the stratigraphic sections of the examined cave. The floor sediments are mineralogically immature, since they contain many ferromagnesian minerals, feldspars (especially plagioclase) and quartz. The extensive presence of silicate minerals means that the phyllites, gneisses, schists, ophiolitic rocks and the clastic Mariam Formation …


Genesis Of Iron And Manganese Sediments In Zoloushka Cave (Ukraine/Moldova) As Revealed By Δ13C Organic Carbon, Piotr Kotula, Viacheslav Andreychouk, Jacek Pawlyta, Leszek Marynowski, Izabela Jendrzejewska Sep 2019

Genesis Of Iron And Manganese Sediments In Zoloushka Cave (Ukraine/Moldova) As Revealed By Δ13C Organic Carbon, Piotr Kotula, Viacheslav Andreychouk, Jacek Pawlyta, Leszek Marynowski, Izabela Jendrzejewska

International Journal of Speleology

Zoloushka Cave is one of the largest maze gypsum caves in the world. Mining of the gypsum bedrock and lowering of the water level due to the pumping of groundwater led to exposure of the cave passages to vadose conditions and changed the hydrochemistry of the karst water. As a result, large quantities of Fe and Mn hydroxides were deposited in the passages. It was found that at least two groups of various organisms were involved in depositing ferrous and manganese sediments. In order to establish the mechanism of deposition, we conducted chemical analyses of the sediments and isotopic analyses …


Full Issue 48(2) Sep 2019

Full Issue 48(2)

International Journal of Speleology

No abstract provided.


Table Of Contents Sep 2019

Table Of Contents

International Journal of Speleology

No abstract provided.


Multi-Phased Hypogene Speleogenesis In A Marginal Horst Structure Of The Malé Karpaty Mountains, Slovakia, Pavel Bella, Pavel Bosák, Petr Mikysek, Juraj Littva, Helena Hercman, Jacek Pawlak Aug 2019

Multi-Phased Hypogene Speleogenesis In A Marginal Horst Structure Of The Malé Karpaty Mountains, Slovakia, Pavel Bella, Pavel Bosák, Petr Mikysek, Juraj Littva, Helena Hercman, Jacek Pawlak

International Journal of Speleology

The Plavecká jaskyňa Cave on the western fault edge of the Malé Karpaty Mountains (western Slovakia) is a result of multi-phased hypogene speleogenesis. It formed in fractured Triassic carbonates by waters ascending along the Vienna Basin Transform Fault between Malé Karpaty Mountains and Záhorská nížina Lowland (the north-eastern part of the Vienna Basin) and/or the N–S-trending faults that intersect it in the cave vicinity. Morphologically, the cave is featured by (1) phreatic chimneys, cupolas, ceiling pockets, enlarged fissures with spongework cavities, upward wall channels and upward oriented large scallops, (2) epiphreatic flat corrosion bedrock floors, feeding fissures and wall water-table …


Are Microclimate Conditions In El Malpais National Monument Caves In New Mexico, Usa Suitable For Pseudogymnoascus Growth?, Terry J. Torres-Cruz, Andrea Porras-Alfaro, Nicole A. Caimi, Ogochukwu Nwabologu, Edward W. Strach, Kaitlyn J.H. Read, Jesse M. Young, Debbie C. Buecher, Diana E. Northup Aug 2019

Are Microclimate Conditions In El Malpais National Monument Caves In New Mexico, Usa Suitable For Pseudogymnoascus Growth?, Terry J. Torres-Cruz, Andrea Porras-Alfaro, Nicole A. Caimi, Ogochukwu Nwabologu, Edward W. Strach, Kaitlyn J.H. Read, Jesse M. Young, Debbie C. Buecher, Diana E. Northup

International Journal of Speleology

White-nose syndrome (WNS) is a bat disease caused by the fungal pathogen Pseudogymnoascus destructans, which thrives in cold and very humid environments where bats frequently hibernate. Conidia of Pseudogymnoascus species are often documented on bats prior to the onset of WNS, but characterization of high-risk areas defined by microclimate cave conditions have been lacking. Investigating the occurrence of this fungal genus and appropriate environmental conditions to support P. destructans in southwestern U.S. caves is key to understanding the sites most likely to be impacted by WNS. Microclimate conditions in ten caves at El Malpais (ELMA) National Monument in New …


Breakdown Mechanisms In Iron Caves. An Example From Brazil, Marcelo R. Barbosa, Allan D.F. Da Silva, Rafael G. De Paula, Georgete M. Dutra, Airton Barata, Iuri V. Brandi, Chrystophe R.P. Da Silva, Robert A. Osborne Jul 2019

Breakdown Mechanisms In Iron Caves. An Example From Brazil, Marcelo R. Barbosa, Allan D.F. Da Silva, Rafael G. De Paula, Georgete M. Dutra, Airton Barata, Iuri V. Brandi, Chrystophe R.P. Da Silva, Robert A. Osborne

International Journal of Speleology

An iron cave in the vicinity of a mine in Carajás, Brazil, was selected to be mined within an assisted elimination project, planned to control all mine advancement operations towards the cave along with a strict speleological physical monitoring. It allowed, in a pioneering way, the recording of events in the cave from the first signs of damage until to the total collapse of the cave. The project lasted four years and it was possible to identify and describe four breakdown mechanisms in iron caves: Fragment downfall, Block downfall, Controlling structure reactivation, and Open discontinuity movement. The mechanisms occurred independently …


The Multifaceted Effects Induced By Floods On The Macroinvertebrate Communities Inhabiting A Sinking Cave Stream, Octavian Pacioglu, Nicoleta Ianovici, Mărioara N. Filimon, Adrian Sinitean, Gabriel Iacob, Henrietta Barabas, Alexandru Pahomi, Andrei Acs, Hanelore Muntean, Lucian Pârvulescu Jul 2019

The Multifaceted Effects Induced By Floods On The Macroinvertebrate Communities Inhabiting A Sinking Cave Stream, Octavian Pacioglu, Nicoleta Ianovici, Mărioara N. Filimon, Adrian Sinitean, Gabriel Iacob, Henrietta Barabas, Alexandru Pahomi, Andrei Acs, Hanelore Muntean, Lucian Pârvulescu

International Journal of Speleology

First-order sinking cave streams experience considerable hydrological variability, including spates and periods of base-flow during dry seasons. Early-summer flooding on a first-order stream sinking in Ciur-Ponor Cave (Romania) represented a suitable opportunity to test the response of the macroinvertebrate community and of basal food resources quantity and diversity to such a disturbance event. The invertebrate community and basal resources (i.e., woody debris, leaves, fine particulate organic matter and epilithon) were collected from three sampling sites, before and after the flood. The sampling strategy followed an up-downstream gradient of both species diversity and quantity of allochtonous organic matter decrease as the …


Species-Area Model Predicting Diversity Loss In An Artificially Flooded Cave In Brazil, Rodrigo L. Ferreira, Thais G. Pellegrini Jul 2019

Species-Area Model Predicting Diversity Loss In An Artificially Flooded Cave In Brazil, Rodrigo L. Ferreira, Thais G. Pellegrini

International Journal of Speleology

Subterranean environments are poorly known regarding many ecological aspects, such as community structure and its response to different disturbances. To estimate the effects of ground area lost in a limestone cave community in Southeastern Brazil, the invertebrate fauna was sampled before 76% of the cave floor was submerged by the filling of a hydroeletric power plant reservoir. Then, a 2-year monitoring was conducted. A species-area curve based on empiric data was constructed and the z-value of the species-area equation was calculated, what allowed estimating the expected cave richness after flooding comparing with data obtained during the monitoring. The results support …


Culture-Based Analysis Of ‘Cave Silver’ Biofilms On Rocks In The Former Homestake Mine In South Dakota, Usa, Amanpreet K. Brar, David Bergmann Jun 2019

Culture-Based Analysis Of ‘Cave Silver’ Biofilms On Rocks In The Former Homestake Mine In South Dakota, Usa, Amanpreet K. Brar, David Bergmann

International Journal of Speleology

Tunnels in a warm, humid area of the 1478 m level of the Sanford Underground Research Facility (SURF), located in a former gold mine in South Dakota, USA, host irregular, thin whitish, iridescent biofilms, which appear superficially similar to ‘cave silver’ biofilms described from limestone and lava tube caves, despite the higher rock temperature (32°C) and differing rock surface (phyllite) present at SURF. In this study, we investigated the diversity of cultivable bacteria constituting the cave silver by using several media: CN agar, CN gellan gum and 0.1X R2A agar. The highest colony count (CFU/g of sample) was observed on …


Comparison Of Some Epigean And Troglobiotic Animals Regarding Their Metabolism Intensity. Examination Of A Classical Assertion, Tatjana Simčič, Boris Sket May 2019

Comparison Of Some Epigean And Troglobiotic Animals Regarding Their Metabolism Intensity. Examination Of A Classical Assertion, Tatjana Simčič, Boris Sket

International Journal of Speleology

This study determines oxygen consumption (R), electron transport system (ETS) activity and R/ETS ratio in two pairs of epigean and hypogean crustacean species or subspecies. To date, metabolic characteristics among the phylogenetic distant epigean and hypogean species (i.e., species of different genera) or the epigean and hypogean populations of the same species have been studied due to little opportunity to compare closely related epigean and hypogean species. To fill this gap, we studied the epigean Niphargus zagrebensis and its troglobiotic relative Niphargus stygius, and the epigean subspecies Asellus aquaticus carniolicus in comparison to the troglobiotic subspecies Asellus aquaticus cavernicolus …


Morphological And Mineralogical Evidence For Ancient Bat Presence In Cova Des Pas De Vallgornera (Llucmajor, Mallorca, Western Mediterranean), Antoni Merino, Joan J. Fornós, Antoni Mulet, Joaquín Ginés May 2019

Morphological And Mineralogical Evidence For Ancient Bat Presence In Cova Des Pas De Vallgornera (Llucmajor, Mallorca, Western Mediterranean), Antoni Merino, Joan J. Fornós, Antoni Mulet, Joaquín Ginés

International Journal of Speleology

Cova des Pas de Vallgornera is a unique karst cave located at the Llucmajor coastal platform that stands out not only because of its length, more than 78 km, but also for its particular morphological suite, richness and variety of speleothems and mineral infillings. Although the mineralogy of speleothems and minerals related to hypogene morphologies has been studied and described, the existence of minerals derived from guano deposits was still poorly investigated. The cave hosted bat colonies until the collapse of its natural entrances, circa 2.4 My ago, since then until its discovery in 1968, the cave remained sealed. These …


Full Issue 48(1) Mar 2019

Full Issue 48(1)

International Journal of Speleology

No abstract provided.


Table Of Contents Mar 2019

Table Of Contents

International Journal of Speleology

No abstract provided.


Guano-Related Phosphate-Rich Minerals In European Caves, Philippe Audra, Jo De Waele, Ilham Bentaleb, Alica Chroňáková, Václav Krištůfek, Ilenia M. D'Angeli, Cristina Carbone, Giuliana Madonia, Marco Vattano, Giovanna Scopelliti, Didier Cailhol, Nathalie Vanara, Marjan Temovski, Jean-Yves Bigot, Jean-Claude Nobécourt, Ermanno Galli, Fernando Rull, Aurelio Sanz-Arranz Mar 2019

Guano-Related Phosphate-Rich Minerals In European Caves, Philippe Audra, Jo De Waele, Ilham Bentaleb, Alica Chroňáková, Václav Krištůfek, Ilenia M. D'Angeli, Cristina Carbone, Giuliana Madonia, Marco Vattano, Giovanna Scopelliti, Didier Cailhol, Nathalie Vanara, Marjan Temovski, Jean-Yves Bigot, Jean-Claude Nobécourt, Ermanno Galli, Fernando Rull, Aurelio Sanz-Arranz

International Journal of Speleology

Guano is a typical deposit found in caves derived from the excretions of bats and in minor cases of birds. These organic deposits decompose and form a series of acid fluids and gases that can interact with the minerals, sediments, and rocks present in the cave. Over sixty phosphates are known and described from caves, but guano decay also often leads to the formation of nitrates and sulfates. In this study twenty-two European caves were investigated for their guano-related secondary minerals. Using various analytical techniques, seventeen phosphates, along with one sulfate (gypsum), were recognized as secondary products of guano decay. …


Cave Dripwater Isotopic Signals Related To The Altitudinal Gradient Of Mount-Lebanon: Implication For Speleothem Studies, Carole Nehme, Sophie Verheyden, Fadi H. Nader, Jocelyne Adjizian-Gerard, Dominique Genty, Kevin De Bont, Benedicte Minster, Ghada Salem, David Verstraten, Philippe Clayes Mar 2019

Cave Dripwater Isotopic Signals Related To The Altitudinal Gradient Of Mount-Lebanon: Implication For Speleothem Studies, Carole Nehme, Sophie Verheyden, Fadi H. Nader, Jocelyne Adjizian-Gerard, Dominique Genty, Kevin De Bont, Benedicte Minster, Ghada Salem, David Verstraten, Philippe Clayes

International Journal of Speleology

An important step in paleoclimate reconstructions based on vadose cave carbonate deposits or speleothems is to evaluate the sensitivity of the cave environment and speleothems to regional climate. Accordingly, we studied four caves, located at different altitudes along the western flank of Mount-Lebanon (Eastern Mediterranean). The objectives of this study are to identify the present-day variability in temperature, pCO2, and water isotopic composition and to assess the possible influence of the altitudinal gradient on cave drip waters and cave streams. We present here an overview of the spatial variability of rainwater based on local and regional data, and …


A Reply To The Comment On “Assessing Preservation Priorities Of Caves And Karst Areas Using The Frequency Of Endemic Cave-Dwelling Species” By Nitzu Et Al. (2018), Int. J. Speleol., 47 (1): 43-52, Eugen I. Nitzu, Ioana N. Meleg, Andrei Giurginca Feb 2019

A Reply To The Comment On “Assessing Preservation Priorities Of Caves And Karst Areas Using The Frequency Of Endemic Cave-Dwelling Species” By Nitzu Et Al. (2018), Int. J. Speleol., 47 (1): 43-52, Eugen I. Nitzu, Ioana N. Meleg, Andrei Giurginca

International Journal of Speleology

No abstract provided.


Comment On “Assessing Preservation Priorities Of Caves And Karst Areas Using The Frequency Of Endemic Cave-Dwelling Species” By Nitzu Et Al. (2018), Int. J. Speleol., 47 (1): 43-52, Oana T. Moldovan, Traian Brad Feb 2019

Comment On “Assessing Preservation Priorities Of Caves And Karst Areas Using The Frequency Of Endemic Cave-Dwelling Species” By Nitzu Et Al. (2018), Int. J. Speleol., 47 (1): 43-52, Oana T. Moldovan, Traian Brad

International Journal of Speleology

No abstract provided.


Cyanobacterial And Algal Abundance And Biomass In Cave Biofilms And Relation To Environmental And Biofilm Parameters, Slađana Popović, Nataša Nikolić, Jelena Jovanović, Dragana Predojević, Ivana Trbojević, Ljiljana Manić, Gordana Subakov Simić Feb 2019

Cyanobacterial And Algal Abundance And Biomass In Cave Biofilms And Relation To Environmental And Biofilm Parameters, Slađana Popović, Nataša Nikolić, Jelena Jovanović, Dragana Predojević, Ivana Trbojević, Ljiljana Manić, Gordana Subakov Simić

International Journal of Speleology

Due to life in extreme environments, cyanobacteria and algae from cave biofilms that form at the entrances or deep inside the cave around artificial lights are of increasing interest to many scientists. It is well-known that many phototrophic microorganisms are first to colonize exposed substrata and produce the organic matter on which other biofilm constituents relly. Many studies dealing with phototrophic microorganisms from biofilms focus on the diversity and community composition of cyanobacteria and algae, while quantitative assessments are rarely implemented. Biofilm sampling was conducted in Degurić and Vernjikica Cave located in Western and Eastern Serbia, respectively. Ecological parameters (temperature, …


Fifty Years Of Cave Arthropod Sampling: Techniques And Best Practices, J. Judson Wynne, Francis G. Howarth, Stefan Sommer, Brett G. Dickson Feb 2019

Fifty Years Of Cave Arthropod Sampling: Techniques And Best Practices, J. Judson Wynne, Francis G. Howarth, Stefan Sommer, Brett G. Dickson

International Journal of Speleology

Ever-increasing human pressures on cave biodiversity have amplified the need for systematic, repeatable, and intensive surveys of cave-dwelling arthropods to formulate evidence-based management decisions. We examined 110 papers (from 1967 to 2018) to: (i) understand how cave-dwelling invertebrates have been sampled; (ii) provide a summary of techniques most commonly applied and appropriateness of these techniques, and; (iii) make recommendations for sampling design improvement. Of the studies reviewed, over half (56) were biological inventories, 43 ecologically focused, seven were techniques papers, and four were conservation studies. Nearly one-half (48) of the papers applied systematic techniques. Few papers (24) provided enough information …


Isolution 1.0: An Isotope Evolution Model Describing The Stable Oxygen (Δ18O) And Carbon (Δ13C) Isotope Values Of Speleothems, Michael Deininger, Denis Scholz Jan 2019

Isolution 1.0: An Isotope Evolution Model Describing The Stable Oxygen (Δ18O) And Carbon (Δ13C) Isotope Values Of Speleothems, Michael Deininger, Denis Scholz

International Journal of Speleology

Stable oxygen and carbon isotope ratios (δ13C and δ18O) are the most applied climate and environmental proxies in speleothems allowing to infer past changes in cave drip water δ13C and δ18O related to climate and environmental variations from above the cave. However, disequilibrium isotope fractionation processes can modify δ13C and δ18O values in speleothems, which is in most cases difficult to estimate due to inter-dependencies on various cave specific parameter. To better understand the effect of these disequilibrium isotope fractionation processes proxy system models were developed in recent …