Digital Commons Network^™

Characterization And Functional Rescue Of Congenital Muscular Dystrophy With Megaconial Myopathy In A Mouse Model Of The Disease, Ambreen A. Sayed

Electronic Theses and Dissertations

Congenital muscular dystrophy with megaconial myopathy (MDCMC) is an autosomal recessive disorder characterized by progressive muscle weakness and wasting. Megamitochondria in skeletal muscle biopsies and cognitive impairments in MDCMC patients are observations exclusive to this type of muscular dystrophy. The disease is caused by loss of function mutations in the choline kinase beta (CHKB) gene which results in dysfunction of the Kennedy pathway for the synthesis of phosphatidylcholine (PC). A rostro-caudal muscular dystrophy (rmd) mouse with a deletion in the Chkb gene resulting in MDCMC-like symptoms has been reported by our lab. In order to test if the rmd mice …

Modeling Human Cancer Therapy Response In Patient Derived Xenografts, Joan Malcolm

Electronic Theses and Dissertations

Patient‐derived xenografts (PDXs) generated by implanting human tumor tissue into a transplant compliant mouse host have been of increasingly importance to preclinical development and have been demonstrated to have advantages compared to cancer cell lines and cell‐line xenografts (CLX) for modeling therapeutic responses in cancer. Nevertheless, many open questions remain regarding the relationship between study design factors and classification of treatment response and the molecular fidelity of tumors passaged in PDXs relative to the original patient tumor(s). The research described in this dissertation addresses both of these significant issues related to the use of PDXs as a tool for modeling …

Host Mediated Mechanisms Of Fungal Cell Spread In A Transparent Zebrafish Infection Model, Allison Scherer

Electronic Theses and Dissertations

Innate immunity has developed elegant processes for the detection and clearance of invasive fungal pathogens. Disseminated candidiasis is of significant concern for those with suppressed immune systems or indwelling medical equipment, and mortality in these groups approaches 70%. Poor patient outcomes have spurred the need to understand how this non-motile pathogen spreads in the host. Technical limitations have previously hindered our ability to visualize the role of innate immunity and host tissue barriers in the spread of C. albicans in vivo. Using the zebrafish model to overcome these limitations, we have examined three potential host-mediated mechanisms of dissemination: movement …

Intergenerational Adult Day Services Needs Assessment Project Final Report, University Of Maine School Of Social Work, Eastern Area Agency On Aging, University Of Maine Center On Aging

Maine Center on Aging Research and Evaluation

Focus group research conducted in the Greater Bangor, Maine area in 2016 identified respite services and intergenerational programming as important factors for supporting a more livable community.

This report outlines findings from a 2018 needs assessment of adult day services (ADS), funded by Maine Health Access Foundation. The needs assessment utilized a survey of local caregivers (N=84) and key informant interviews (N=10) with staff at Maine adult day service programs or service providers that could utilize adult day services for their clients.

Key survey findings indicate that lack of financial resources (identified by 20% of the survey sample), and lack …

The Effect Of Wild Blueberry Bioactives On Endothelial Cell Migration And Angiogenesis: An In Vitro Mechanistic, Genomic And Proteomic Approach, Panagiotis Tsakiroglou

Electronic Theses and Dissertations

The goal of this study is to investigate the effects of wild blueberry fractions (Anthocyanins and Phenolic acids) on vascular function and physiology. More specifically the potential effects of the above fractions and their combination in physiological concentrations on endothelial cell migration, angiogenesis, gene expression and proteins synthesis of markers related to the above processes. The objectives are to study whether anthocyanins, phenolic acids and their combinations (ACNs:PAs) affect: a) cell proliferation, b) speed of endothelial cell migration, c) angiogenesis, d) gene expression of genes critical for cell migration and angiogenesis such as RAC1, RHOA, AKT1, eNOS and VEGF and …

Genetic Modification Of Inherited Retinopathy In Mice, Yang Kong

Electronic Theses and Dissertations

The retina, as a critical component of the sensory system, consists of multiple cell types, of which, photoreceptors play a key role in receiving, integrating and transmitting light signals. The biofunctions of photoreceptors rely on their proper growth and development, which is predominantly governed by a cluster of molecules that comprise the transcriptional regulation for photoreceptor development. Any disruption of these molecules potentially incurs retinal pathologies.

It is known that deficiencies of nuclear receptor subfamily 2 group E member 3 (NR2E3) or neural retina leucine-zipper (NRL), two molecules in regulating photoreceptor cell development, cause photoreceptor dysplasia. In a sensitized chemical …

The Efficacy Of Equine Assisted Therapy In The Treatment Of Autism Spectrum Disorders, Amy Hofmann

Electronic Theses and Dissertations

This study examines the effects of equine-assisted psychotherapies in children with an autism spectrum disorder. The CARS-2 and Children’s Sleep Habits Questionnaire parent-report questionnaires were used for evaluation, as well open-ended questions. A single researcher contacted and visited many PATH-certified centers in the United States. Facilities that participated were all located in the Mid-Atlantic to Northeast region. There were 16 participants, from 11 different farms, that completed both the initial and follow-up questionnaires which were given 7 weeks apart. A $25 Amazon gift card was used as an incentive to increase participation. Participating facilities also completed a questionnaire.

Overall, results …

The Effect Of Wild Blueberry Anthocyanins And Phenolic Acids On Innate Immunity: The Compliment System, Vasiliki Papakotsi

Electronic Theses and Dissertations

Ingestion of berries containing polyphenols is associated with lower risk of inflammatory, metabolic, cardiovascular and degenerative diseases. Diet has been shown to modulate the activation of the complement system, a set of over 50 proteins present in the circulation and tissues that reacts in response to damage or microbial encounter and is critical for the maintenance of homeostasis. Imbalanced activation is tightly correlated with inflammation and various pathologies. Wild blueberries are a rich source of anthocyanins and phenolic acids, which can be found in plasma shortly after consumption. Given the involvement of both complement and polyphenols in the modulation of …

The Efficiency Of Melatonin In Inhibiting Haemonchus Contortus Development, Stephanie M. Mcavoy

Honors College

Haemonchus contortus (HC) is a pathogenic nematode that causes sheep morbidity through anemia, edema, and weight loss. It’s the most economically devastating parasite to sheep industries, with increasing global temperatures and anthelmintic resistance increasing HC residing areas and life spans. HC enter hypobiosis, a state comparable to hibernation during the fall when day length decreases. In this state of arrested development, the sheep are not taxed. Hypobiosis occurs in late fall and early winter. Melatonin, with its release having a direct relationship to length of night, is highest during this period. Combining melatonin’s relationship to the seasons, along with research …

Context Effects On Ambiguous Idiom Comprehension In Older And Younger Adults, Amy De Silva

Honors College

The focus of the current study was on idiom comprehension in younger and older adults. Due to inconsistent results in previous studies, it is unclear whether older adults may have problems understanding idioms. For the current study, I used a sentence-to-word matching task presented on an iPad with software that recorded participants’ response time and accuracy. Participants also completed a familiarity task where they rated idioms on how frequently these phrases were encountered. I predicted that older adults would have more difficulty comprehending idioms because of the context in which the idioms were embedded and the timed nature of the …

Characterizing The Role Of Fungal Shape In A Zebrafish Model Of Invasive Candidiasis, Brittany Seman

Electronic Theses and Dissertations

Candida albicans is a common hospital-acquired fungal infection, and disseminated disease claims up to one-half of those afflicted. C. albicans has a unique ability to differentiate its shape during infection, and this differentiation is thought to be a major virulence factor during invasive infections. Each shape is proposed to have a specialized role: filaments drive tissue invasion and yeast mediate dissemination to the bloodstream. However, it has been difficult to test these hypotheses for two reasons. First, rigorous testing of shape-specific roles requires diverse strategies of shape modulation that restrict the possibility of manipulation-specific artifacts. Second, although connecting shape to …

Examining The Relationship Between The Use Of Simulation In Nursing Education Andsafety With Medication Adminstration In The Clinical Setting, Deborah A. Eremita

Electronic Theses and Dissertations

EXAMINING THE RELATIONSHIP BETWEEN THE USE OF SIMULATION IN

NURSING EDUCATION AND SAFETY WITH

MEDICATION ADMINISTRATION

IN THE CLINICAL SETTING

Deborah A. Eremita

Thesis Advisors: Dr. Ann Sossong and Dr. Patricia Poirier

An Abstract of the Dissertation Presented

in Partial Fulfillment of the Requirements for the

Degree Interdisciplinary Doctor of Philosophy

In Nursing and Education

May 2018

Medication errors represent a significant threat to patient safety. Administration of medications is a primary role of nursing practice and a critical component of nursing education curricula. Safe medication is a challenging process to teach nursing students. Simulation may provide students with a …

Developing A Risk Assessment Protocol To Quantify Distribution And Uptake Of Persistent Organic Pollutants In Glacial Outflows, Kimberley Rain Miner

Electronic Theses and Dissertations

Pollutants released by industrialized nations between 1960 and 2004 have been transported northward through atmospheric processes and deposited into glaciated alpine ecosystems. Many of these chemicals retain their original structure and are absorbed into the biota thousands of miles away from where they were originally utilized. With a warming climate increasing the melt of alpine glaciers, these glaciers may be introducing growing amounts of toxins into the watershed. While studies have demonstrated the existence of resident pollutants within glaciated ecosystems, no one has developed a risk assessment to identify sources and quantity of risk posed by these compounds when released …

Extraction And Purification Of (E)-Resveratrol From The Bark Of Maine's Native Spruces, Panduka S. Piyaratne

Electronic Theses and Dissertations

Resveratrol is a plant secondary metabolite which protects plants from pathogenic invasions and acts as a remedial agent against injury, stress, and tissue damage by UV radiation. With its characteristic stilbene structure, resveratrol adopts (E) and (Z) isomeric forms. However, the (E) isomer is the most biologically active and abundant form of resveratrol in nature.

(E)- Resveratrol confers a variety of benefits to human health and well-being. Besides its antioxidant activity, the role of (E)-resveratrol as an anti-inflammatory, anti-aging, cardioprotection, and anticancer agent has been a widely explored research interest. …

Precision Gene Therapy For Charcot-Marie-Tooth Disease: From Identifying Genetic Modifiers To Developing Allele-Specific Therapies, Kathryn H. Morelli Ph.D.

Electronic Theses and Dissertations

Charcot-Marie-Tooth Disease (CMT) is a clinically and genetically heterogeneous collection of inherited peripheral neuropathies generally characterized by progressive muscle atrophy, weakness, and loss of sensation in the distal extremities. This inherited disorder, for which there is currently no curative treatment, is the most common inherited disease of the peripheral nervous system, affecting 1:2,500 individuals worldwide.

Clinically, CMT is broadly divided into demyelinating (type 1) and axonal (type 2) forms. Although the clinical presentation can vary greatly in severity and progression within individual patients. Genetically, over 1,000 mutations in over 80 loci in the human genome have been linked to specific …

Early Intervention Speech-Language Pathologists: A Systematic Review, Margaret M. Pierce

Electronic Theses and Dissertations

Purpose: The purpose of this Thesis is to describe the current supporting evidence base for speech-language pathologists working within early intervention with the birth through two-years-old population. Aims: The aims of this systematic review are to determine: (a) the current evidence base of early intervention by speech-language pathologists with infants and toddlers and (b) the quality of research available. Method: An exhaustive systematic review method is used. Search terms are based on subject or index headings related to the aims of this systematic review, i.e. early intervention and speech-language pathologists. A screening method is used to identify eligible publications for …

Improving Techniques To Study Equine Cervical Mucociliary Clearance, Melissa A. Hawkes

Electronic Theses and Dissertations

Bacterial uterine infections inflict major losses on the equine breeding industry. These infections usually arise from bacteria introduced at breeding. Micro-currents propelled by ciliated cells between the folds of the uterus and cervix have been proposed as a means by which contaminants are expelled. Previous data have shown possible ciliary micro-currents propelling carbon particles, occasionally rotating, through cervical folds. However, adherence to the epithelium may have interfered with movement of carbon in these studies. Therefore, we tested potentially non-adherent substances to reveal ciliary micro-currents on the equine cervix under high magnification video-endoscopy. We hypothesized that polyethylene green microspheres 1 - …

Designing A Stress Free Vet Visit: A Systematic Review, Naedia Clarke

Honors College

The purpose of this study was to create a systematic review of articles pertaining to stress measurement and management of dogs and cats in order to use the information to find a way to reduce stress associated with a visit to the veterinarian. The corpus for this review contains 42 articles with 33 primary research studies and 9 secondary research studies. The information deduced during the review showed that a majority of studies on animal stress take place outside of the USA. The review also showed that a visit to a vet practice begins with client-staff communication. This collected knowledge …

A Characterization Of Poultry Flocks And Poultry Producers In Maine, Alice Gluchanicz

Honors College

This study sought to characterize backyard poultry flocks and poultry producers’ knowledge of poultry husbandry in Maine. A survey on poultry flock characterization, management, and health was sent to poultry producers living in Maine. Based on the survey, most backyard poultry flocks contained 7-20 birds. Chickens were the predominant poultry species in Maine. Flocks were used primarily for egg production and companionship. Management practices varied greatly among producers, indicating a need for more flock management education. Mites and coccidiosis were the most commonly reported causes of illness by producers, but several diseases affected poultry in Maine. Producers cited the internet …

Language Modality For Deaf Or Hard Of Hearing Preschoolers: Maine Parents' Decision-Making, Sarah Basquez

Honors College

Both parental decision-making and the choice of language modality for Deaf and Hard of Hearing (DHH) children have been studied. In investigating the two subjects in conjunction with a bilingual bimodal (bi-bi) program, however, there is an apparent lack of research. A preschool program featuring bi-bi in Maine, named the Maine Educational Center for the Deaf and Hard of Hearing (MECDHH), prides itself on the fact that parents determine their child’s primary language upon enrollment. Although many bi-bi programs exist worldwide, the MECDHH program is unique in that the preschoolers are separated into two different classrooms: one featuring solely orally …

The Importance Of Medicaid For Children With Disabilities And Special Health Care Needs, 2017-2018 Cohort Of New Hampshire-Maine Leadership Education In Neurodevelopmental And Related Disabilities (Nh-Me Lend) Program Trainees.

Policy Analysis

Changes to Medicaid financing structure should be carefully considered because any change could have a potentially negative impact on children with disabilities and special health care needs (hereafter referred to as children with disabilities) and limit their access to critically needed health care services and community supports. Currently, Medicaid funding operates through a state and federal partnership, with the federal government providing 60% of funding on average. A cornerstone of Medicaid is the Early and Periodic Screening, Diagnostic, and Treatment (EPSDT) child health benefit. This program offers vital support services to children with disabilities, including early and periodic screenings, comprehensive …