Digital Commons Network^™

P5l Mutation In Ank Results In An Increase In Extracellular Inorganic Pyrophosphate During Proliferation And Nonmineralizing Hypertrophy In Stably Transduced Atdc5 Cells, Raihana Zaka, David Stokes, Arnold S. Dion, Anna Kusnierz, Fei Han, Charlene J. Williams

Charlene Williams

Ank is a multipass transmembrane protein that regulates the cellular transport of inorganic pyrophosphate. In the progressive ankylosis (ank) mouse, a premature termination mutation at glutamic acid 440 results in a phenotype characterized by inappropriate deposition of basic calcium phosphate crystals in skeletal tissues. Mutations in the amino terminus of ANKH, the human homolog of Ank, result in familial calcium pyrophosphate dihydrate deposition disease. It has been hypothesized that these mutations result in a gain-of-function with respect to the elaboration of extracellular inorganic pyrophosphate. To explore this issue in a mineralization-competent system, we stably transduced ATDC5 cells with wild-type Ank …

Serine-Dependent Sphingolipid Synthesis Is A Metabolic Liability Of Aneuploid Cells, Sunyoung Hwang, H. Tobias Gustafsson, Ciara O’Sullivan, Gianna Bisceglia, Xinhe Huang, Christian Klose, Andrej Schevchenko, Robert C. Dickson, Paola Cavaliere, Noah Dephoure, Eduardo M. Torres

Molecular and Cellular Biochemistry Faculty Publications

Aneuploidy disrupts cellular homeostasis. However, the molecular mechanisms underlying the physiological responses and adaptation to aneuploidy are not well understood. Deciphering these mechanisms is important because aneuploidy is associated with diseases, including intellectual disability and cancer. Although tumors and mammalian aneuploid cells, including several cancer cell lines, show altered levels of sphingolipids, the role of sphingolipids in aneuploidy remains unknown. Here, we show that ceramides and long-chain bases, sphingolipid molecules that slow proliferation and promote survival, are increased by aneuploidy. Sphingolipid levels are tightly linked to serine synthesis, and inhibiting either serine or sphingolipid synthesis can specifically impair the fitness …

In-Vitro And In-Vivo Models Of Bile Acid Metabolism And Transport, Rhishikesh Thakare

Theses & Dissertations

All biomedical research is conducted in animal models first. In addition, the Food and Drug Administration requires extrapolation from animal data to predict human responses. There are ongoing scientific and regulatory challenges translating interspecies comparisons and predictions. Metabolic pathways are a cornerstone to understanding drug metabolism and toxicities and the liver is a key organ in this process. Bile acids (BAs) play a central role in the hepatobiliary toxicities of chemicals, toxins, and biological reagents. BAs have many physiological functions including regulation of genes involved in cholesterol and glucose metabolism and BA homeostasis. However, BAs also have several pathological effects …

Regulation Of Liver Mitochondrial Metabolism During Hibernation By Post-Translational Modification, Katherine E. Mathers

Electronic Thesis and Dissertation Repository

Hibernation, characterized by a seasonal reduction in metabolism and body temperature, allows animals to conserve energy when environmental conditions (e.g. temperature, food availability) are unfavourable. During hibernation, small mammals such as the 13-lined ground squirrel (Ictidomys tridecemlineatus) cycle between two distinct metabolic states: torpor, where metabolic rate is suppressed by >95% and body temperature falls to ~5 °C, and interbout euthermia (IBE), where metabolic rate and body temperature rapidly increase and are maintained at euthermic levels several hours. Suppression of metabolism during entrance into torpor is paralleled by rapid suppression of liver mitochondrial metabolism. In my thesis, I …

Lipid Utilization Of Pseudogymnoascus Destructans And Accompanying Physiological Factors That Influence White-Nose Syndrome, John Neville

Biology Dissertations

Pseudogymnoascus destructans is the causative agent of white-nose syndrome in bats and since its discovery in 2006 it is known to have caused the death of over 5.5 million bats in North America. As with all diseases there is a cycle that perpetuates the disease at the cost of the host’s health. Currently, the majority of the research into this disease has been focused on the host organism. Therefore, in this research with the rational to further elucidate the complete disease cycle we concentrate on the pathogen with specific focus on its ability to utilize lipids found in bat wing …

Type 1 Diabetes Alters Lipid Handling And Metabolism In Human Fibroblasts And Peripheral Blood Mononuclear Cells, Albert R. Jones Iv, Emily L. Coleman, Nicholas R. Husni, Jude T. Deeney, Forum Raval, Devin Steenkamp, Hans Dooms, Barbara S. Nikolajczyk, Barbara E. Corkey

Clinical and Translational Science Faculty Publications

Triggers of the autoimmune response that leads to type 1 diabetes (T1D) remain poorly understood. A possibility is that parallel changes in both T cells and target cells provoke autoimmune attack. We previously documented greater Ca²⁺ transients in fibroblasts from T1D subjects than non-T1D after exposure to fatty acids (FA) and tumor necrosis factor α (TNFα). These data indicate that metabolic and signal transduction defects present in T1D can be elicited ex vivo in isolated cells. Changes that precede T1D, including inflammation, may activate atypical responses in people that are genetically predisposed to T1D. To identify such cellular differences …

The Toxicity Of Nanoparticles Depends On Multiple Molecular And Physicochemical Mechanisms, Yue-Wern Huang, Melissa Cambre, Han-Jung Lee

Biological Sciences Faculty Research & Creative Works

Nanotechnology is an emerging discipline that studies matters at the nanoscale level. Eventually, the goal is to manipulate matters at the atomic level to serve mankind. One growing area in nanotechnology is biomedical applications, which involve disease management and the discovery of basic biological principles. In this review, we discuss characteristics of nanomaterials, with an emphasis on transition metal oxide nanoparticles that influence cytotoxicity. Identification of those properties may lead to the design of more efficient and safer nanosized products for various industrial purposes and provide guidance for assessment of human and environmental health risk. We then investigate biochemical and …

Comparison Of Ecosystem Processes In A Woodland And Prairie Pond With Different Hydroperiods, Daniel J. Hornbach, Mark C. Hove, Mira W. Ensley-Field, Matthew R. Glasenapp, Ian A. Goodbar, J. Douglas Harman, Benjamin D. Huber, Emily A. Kangas, Kira X. Liu, Molly Stark-Ragsdale, Long K. Tran

Faculty Publications

Shallow lakes and ponds constitute a significant number of water bodies worldwide. Many are heterotrophic, indicating that they are likely net contributors to global carbon cycling. Climate change is likely to have important impacts on these waterbodies. In this study, we examined two small Minnesota ponds; a permanent woodland pond and a temporary prairie pond. The woodland pond had lower levels of phosphorus and phytoplankton than the prairie pond. Using the open water oxygen method, we found the prairie pond typically had a higher level of gross primary production (GPP) and respiration (R) than the woodland pond, although the differences …

A License To Kill: Understanding Natural Killer Cell Licensing To Fight Cancer, Jolie Schafer

Dissertations & Theses (Open Access)

Natural killer (NK) cell education is an essential developmental process for NK cell effector function, that renders some NK cells “licensed” and others “unlicensed” (with heightened or lowered effector function potential, respectively) against tumor and targets lacking self-molecules. However, the underlying mechanisms responsible for the heightened effector responses of licensed cells remain unknown. Using NK cells derived from humans and expanded ex vivo we performed high-throughput protein expression analysis, and identified multiple proteins that are differentially regulated in licensed and unlicensed human NK cells before and after inhibition by killer-cell immunoglobulin-like receptors (KIRs) and activation by the NKp46 natural cytotoxicity …

Delayed Effects Of Sub-Lethal Lampricide, 3-Trifluoromethyl-4-Nitrophenol (Tfm), Exposure On The Metabolic Physiology Of Juvenile Lake Sturgeon (Acipenser Fulvescens), Matthew J. Symbal

All NMU Master's Theses

The lampricide 3-trifluoromethyl-4-nitrophenol (TFM) can be lethal to juvenile lake sturgeon (Acipenser fulvescens) at high concentrations used to control invasive sea lamprey (Petromyzon marinus). Little is known about how TFM would affect juvenile lake sturgeon (age-0) at the sub-lethal concentrations encountered during routine lampricide treatments; specifically, its effects on growth, stress response, and metabolism. Age-0 lake sturgeon were exposed to TFM concentrations at levels 0.0x, 0.5x, and 1.0x the minimum lethal concentration (mlc) of TFM required to kill 99.9% of sea lamprey larvae during a routine stream treatment. The mlc was estimated based on pH and alkalinity …

Effect Of Temperature And Wind On Metabolism Of Northern Bobwhite In Winter, L. Wes Burger Jr., Thomas V. Dailey, Mark R. Ryan, Eric Kurzejeski

National Quail Symposium Proceedings

Northern bobwhite (Colinus virginianus) are widely distributed across more than half of the United States, and extending into Canada and Mexico. Within this distribution they tolerate a wide range of climatic conditions and thermal stress. Annual variation in weather can produce dramatic short-term population fluctuations, particularly in the northern portion of the distribution. To better understand effects of thermal stress on energy requirements of bobwhite, we measured roosting metabolic response to cold stress and wind speed using open respirometry in a closed-circuit wind tunnel. Oxygen consumption was measured for 8 winter-acclimated captive bobwhites at each of 8 temperatures …

Mitochondrial Reactive Oxygen Species In Lipotoxic Hearts Induces Post-Translational Modifications Of Akap121, Drp1 And Opa1 That Promote Mitochondrial Fission, Kensuke Tsushima, Heiko Bugger, Adam R. Wende, Jamie Soto, Gregory A. Jenson, Austin R. Tor, Rose Mcglauflin, Helena C. Kenny, Yuan Zhang, Rhonda Souvenir, Xiao X. Hu, Crystal L. Sloan, Renata O. Pereira, Vitor A. Lira, Kenneth W. Spitzer, Terry L. Sharp, Kooresh I. Shoghi, Genevieve C. Sparagna, Eva A. Rog-Zielinska, Peter Kohl, Oleh Khalimonchuk, Jean E. Schaffer, E. Dale Abel

Department of Biochemistry: Faculty Publications

Rationale: Cardiac lipotoxicity, characterized by increased uptake, oxidation and accumulation of lipid intermediates, contributes to cardiac dysfunction in obesity and diabetes. However, mechanisms linking lipid overload and mitochondrial dysfunction are incompletely understood.

Objective: To elucidate the mechanisms for mitochondrial adaptations to lipid overload in postnatal hearts in vivo.

Methods and Results: Using a transgenic mouse model of cardiac lipotoxicity overexpressing long-chain acyl-CoA synthetase 1 in cardiomyocytes, we show that modestly increased myocardial fatty acid uptake leads to mitochondrial structural remodeling with significant reduction in minimum diameter. This is associated with increased palmitoyl-carnitine oxidation and increased reactive oxygen species (ROS) generation …

Reach-Scale River Metabolism Across Contrasting Sub-Catchment Geologies: Effect Of Light And Hydrology, Lorenzo Rovelli, Karl M. Attard, Andrew Binley, Catherine M. Heppell, Henrik Stahl, Mark Trimmer, Ronnie N. Glud

All Works

© 2017 The Authors Limnology and Oceanography published by Wiley Periodicals, Inc. on behalf of Association for the Sciences of Limnology and Oceanography We investigated the seasonal dynamics of in-stream metabolism at the reach scale (∼ 150 m) of headwaters across contrasting geological sub-catchments: clay, Greensand, and Chalk of the upper River Avon (UK). Benthic metabolic activity was quantified by aquatic eddy co-variance while water column activity was assessed by bottle incubations. Seasonal dynamics across reaches were specific for the three types of geologies. During the spring, all reaches were net autotrophic, with rates of up to 290 mmol C …

Physiological Ecology Of Four Endemic Alabama Species And The Exotic Asiatic Weatherfish, Misgurnus Anguillicaudatus (Cantor, 1842), Lindsay M. White, Mark E. Meade, Benjamin A. Staton

Southeastern Fishes Council Proceedings

The occurrence of Asiatic Weatherfish, Misgurnus anguillicaudatus, in Alabama, a state known for its rich biodiversity, has generated concern among conservation managers. The current study used respirometry techniques to investigate the effects of increasing temperature on four native southeastern fishes (one cyprinid, two percids, and one elassomid) and the non-native M. anguillicaudatus. A minimum of five individuals of each species were used, and three experimental temperatures were chosen to represent spring and summer averages of northeast Alabama streams (15, 20, and 25°C). Overall, mean standard metabolic rates (SMRs) for M. anguillicaudatus were low (97.01, 127.75, and 158.50 mg …

Loss Of Fructose-1,6-Bisphosphatase Induces Glycolysis And Promotes Apoptosis Resistance Of Cancer Stem-Like Cells: An Important Role In Hexavalent Chromium-Induced Carcinogenesis, Jin Dai, Yanli Ji, Wei Wang, Donghern Kim, Leonard Yenwong Fai, Lei Wang, Jia Luo, Zhuo Zhang

Toxicology and Cancer Biology Faculty Publications

Hexavalent chromium (Cr(VI)) compounds are confirmed human carcinogens for lung cancer. Our previous studies has demonstrated that chronic exposure of human bronchial epithelial BEAS-2B cells to low dose of Cr(VI) causes malignant cell transformation. The acquisition of cancer stem cell-like properties is involved in the initiation of cancers. The present study has observed that a small population of cancer stem-like cells (BEAS-2B-Cr-CSC) exists in the Cr(VI)-transformed cells (BEAS-2B-Cr). Those BEAS-2B-Cr-CSC exhibit extremely reduced capability of generating reactive oxygen species (ROS) and apoptosis resistance. BEAS-2B-Cr-CSC are metabolic inactive as evidenced by reductions in oxygen consumption, glucose uptake, ATP production, and lactate …

Regulation Of Cardiac Hypertrophy And Metabolism By Regulator Of G Protein Signalling 2 (Rgs2), Katherine N. Lee

Electronic Thesis and Dissertation Repository

Pathological left ventricular hypertrophy is a maladaptive cardiomyocyte growth response to various cardiovascular conditions such as hypertension, and is a major risk factor for heart failure and stroke. The majority of drugs used to treat cardiovascular diseases target G protein coupled receptors (GPCRs), which are regulated by regulator of G protein signalling (RGS) proteins. RGS2 is a GTPase activating protein which limits G_q- and G_s-mediated signalling, which are known to play major roles in the development of pathological cardiac hypertrophy. In addition to its G protein effects, we have previously shown that RGS2 can also inhibit …

Investigating The Protective Effects Of Telomerase Reverse Transcriptase On Neuronal Metabolism And Resistance To Amyloid-Beta, Olivia Singh

Electronic Thesis and Dissertation Repository

Maintenance of telomere length during cell division is dependent on the catalytic subunit telomerase reverse transcriptase (TERT), which adds TTAGGG repeats to the ends of chromosomes to prevent telomere shortening during DNA replication. However, non-telomeric roles of TERT have emerged under oxidative stress whereby TERT translocates from the nucleus to the mitochondria and protects against mitochondrial dysfunction through a poorly defined mechanism. A major pathological feature of Alzheimer’s Disease (AD) is the progressive accumulation of amyloid-beta (Aβ) peptide within the cortex and hippocampus. Aβ can directly interfere with mitochondrial respiration and promote mitochondrial dysfunction, ROS production, and neuronal cell death. …

Another Route For Amino Acid Production?: Reverse Genetic Probing For A Functional Cytosolic Shikimate Pathway In Plants, Gabrielle C. Buck, Joseph Lynch, Natalia Dudareva

The Summer Undergraduate Research Fellowship (SURF) Symposium

The shikimate pathway is a metabolic pathway that produces the three aromatic amino acids—phenylalanine, tryptophan, and tyrosine—which are essential to human diets and necessary for many plant functions. Consequently, the shikimate pathway is commonly targeted for antibiotic and herbicide strategies as well as genetic engineering in several fields. This pathway is known to be localized in the plastids, or double membrane-bound organelles, of plant cells; however, there is enzymatic evidence of another shikimate pathway in the cell fluid, or cytosol. To determine whether a complete cytosolic shikimate pathway exists, we used a modified gene for the first enzyme of the …

Acetate Metabolism: The Physiological Role Of Adp-Forming Acetyl-Coa Synthetase And Acetate Kinase In Entamoeba Histolytica, Thanh Dang

All Dissertations

Entamoeba histolytica is a protozoan parasite that causes amoebic colitis and liver abscess in approximately 90 million people each year, resulting in 50,000-100,000 fatalities. Even though Entamoeba poses a significant public health problem worldwide, research dedicated to understanding the biology of this unique protozoan has been limited. This amitochondriate parasite lacks many essential biosynthesis pathways including the tricarboxylic acid (TCA) cycle and oxidative phosphorylation. As a result, substrate level phosphorylation plays a necessary role in ATP production. Unlike the standard glycolytic pathway, E. histolytica glycolysis requires pyrophosphate (PPi) by replacing ATP - dependent phosphofructokinase and pyruvate kinase with PPi - …

Metabolic Biomarkers Following A Short And Long Bout Of High-Intensity Functional Training In Recreationally Trained Men, Brian Kliszczewicz, Robert Buresh, Emily E. Bechke, Cassie M. Williamson

Faculty Articles

Glucose regulation is a fundamental process of metabolic function, and is acutely altered by physical activity. High-Intensity Functional Training (HIFT) is a form of exercise performed using combinations of various modalities and durations. It is unknown if the metabolic responses to HIFT are similar to more commonly studied modalities (e.g., cycling and treadmill exercise), or if exercise duration will influence glucose regulation. The purpose of this study was to determine the effect of a Short (< 5-min) and Long (15-min) bout of HIFT on plasma metabolic biomarkers. Ten apparently healthy males (28.11 ± 5.09yrs) participated in this study. Two HIFT sessions (SHORT and LONG) were performed in a crossover fashion. Blood plasma was collected at four time points: PRE, POST, 1HR, and 3HR in order to examine glucose (GLU), insulin (INS), epinephrine (E), and norepinephrine (NE) responses. No trial dependent difference between the SHORT and LONG bouts in GLU (p=0.054), INS (p=0.671), E (p=0.078), and NE (p=0.194). A time effect was observed in both bouts only at POST for GLU (p<0.001), INS (p=0.011), E (p<0.001), and NE (p<0.001). All times returned to baseline values (p>0.05), except for lowered 3HR E (p=0.007). This study demonstrated that both SHORT and LONG bouts of HIFT elicited GLU, INS, E, and NE …

Metabolic Regulation Of Cellular Signaling, Rashid John Darbandi

Theses and Dissertations (ETD)

Using the biochemically tractable Xenopus oocyte model system, we have previously characterized a novel metabolic regulation of cell death. We found that glucose-6-phosphate (G6P) via the pentose phosphate pathway leads to increased nicotinamide adenine dinucleotide phosphate (NADPH) levels, a subsequent increase in cytosolic acetyl-coenzyme A and activation of Ca2+/calmodulin-dependent protein kinase II (CaMKII). We recently identified coenzyme A (CoA), derived from the breakdown of acetyl-CoA, as the key metabolic signal that mediates a novel mechanism of calmodulindependent activation of CaMKII. CoA binds directly to the calmodulin (CaM) binding domain (CaMBD) of CaMKII resulting in its activation and downstream inhibitory phosphorylation …

Biotransformation Of Polymethoxyflavones And Its Implication On Biological Activities, Minqi Wang

Doctoral Dissertations

In this thesis, the tissue distribution and in vivo biotransformation of nobiletin in rodents were demonstrated on three types of rodents, i.e.A-J female mice, CD-1 male mice and male F344 male rats. The effects of colonic metabolites of CD-1 mice on colonic carcinogenesis and inflammation were determined. In order to mimic the long-term dietary consumption of PMFs from food or dietary supplements, we applied oral administration by mixing PMFs with standard diet to rodents who have free access to food. Moreover, nobiletin (NBT), one of the most abundant PMFs in nature, and its major metabolties were incubated with mice …

Exploring Cancer Metabolism Using Stable Isotope-Resolved Metabolomics (Sirm), Ronald C. Bruntz, Andrew N. Lane, Richard M. Higashi, Teresa W. -M. Fan

Center for Environmental and Systems Biochemistry Faculty Publications

Metabolic reprogramming is a hallmark of cancer. The changes in metabolism are adaptive to permit proliferation, survival, and eventually metastasis in a harsh environment. Stable isotope-resolved metabolomics (SIRM) is an approach that uses advanced approaches of NMR and mass spectrometry to analyze the fate of individual atoms from stable isotope-enriched precursors to products to deduce metabolic pathways and networks. The approach can be applied to a wide range of biological systems, including human subjects. This review focuses on the applications of SIRM to cancer metabolism and its use in understanding drug actions.

Editorial: Metabolic Control Of Brain Homeostasis, Detlev Boison, Jochen C. Meier, Susan A. Masino

Faculty Scholarship

No abstract provided.

Regulation Of Mtorc1 By Homocysteine And Its Effects On Autophagy In Human And Mouse Neuronal Tissues, Khoosheh Khayati

Dissertations

The molecular mechanisms leading to and responsible for age-related, sporadic Alzheimer’s disease (AD) remain largely unknown. It is well documented that aging patients with elevated levels of the amino acid metabolite homocysteine (Hcy) are at high risk of developing AD. The impact of Hcy on molecular clearance pathways in mammalian cells, including in-vitro cultured induced pluripotent stem cell-derived forebrain neurons and in-vivo neurons in mouse brains is investigated in this research project. Exposure to high Hcy levels results in up-regulation of the mechanistic target of rapamycin complex 1 (mTORC1) activity, one of the major kinases in cells that is tightly …

Evolutionary Enhancement Of Zika Virus Infectivity In Aedes Aegypti Mosquitoes, Yang Liu, Jianying Liu, Senyan Du, Cheng-Feng Qin, Penghua Wang, Pei-Yong Shi, Gong Cheng

NYMC Faculty Publications

Zika virus (ZIKV) remained obscure until the recent explosive outbreaks in French Polynesia (2013-2014) and South America (2015-2016). Phylogenetic studies have shown that ZIKV has evolved into African and Asian lineages. The Asian lineage of ZIKV was responsible for the recent epidemics in the Americas. However, the underlying mechanisms through which ZIKV rapidly and explosively spread from Asia to the Americas are unclear. Non-structural protein 1 (NS1) facilitates flavivirus acquisition by mosquitoes from an infected mammalian host and subsequently enhances viral prevalence in mosquitoes. Here we show that NS1 antigenaemia determines ZIKV infectivity in its mosquito vector Aedes aegypti, which …

The Effect Of Betaine On Nitrate And Cardiovascular Response To Exercise, J. Luke Pryor, Stephen A. Wolf, Gary Sforzo, Tom Swensen

International Journal of Exercise Science

International Journal of Exercise Science 10(4): 550-559, 2017. Betaine (BT) supplementation improves selected markers of physical performance, however, the mechanism(s) by which this change occurs remains largely unknown. Some speculate that BT may increase circulating nitrate concentrations, improving physical performance by augmentation of endothelial nitric oxide production. The purpose of this study was to investigate the effects of acute BT supplementation and exercise on plasma nitrate levels and related cardiovascular response (CVR). Placebo and BT trials were administered in a cross-over, randomized, double-blind, and counterbalanced fashion. Ten healthy college-aged volunteers consumed either a 250 ml placebo (carbohydrate-electrolyte beverage, CHO) or …

A Genetic Analysis Of Nuclear Functions Of The Lipin Protein In Drosophila Melanogaster, Xeniya Rudolf

Graduate Theses and Dissertations

Lipins are a family of proteins that have critical functions in the control of fat storage and energy homeostasis. Biochemically, lipins have two functions. They provide an enzymatic activity (phosphatidate phosphatase or PAP activity) in the glycerol-3 phosphate pathway that leads to the production of storage fats (triacylglycerols). In addition, they play a role in the regulation of genes in the cell nucleus as transcriptional co-regulators. The PAP activity of lipins has been widely studied in a number of organisms. However, the transcriptional co-regulator function is not as well described in the literature. The transcriptional function of lipins depends on …

Processes On The Emergent Landscapes Of Biochemical Reaction Networks And Heterogeneous Cell Population Dynamics: Differentiation In Living Matters., Sui Huang, Fangting Li, Joseph X Zhou, Hong Qian

Articles, Abstracts, and Reports

The notion of an attractor has been widely employed in thinking about the nonlinear dynamics of organisms and biological phenomena as systems and as processes. The notion of a landscape with valleys and mountains encoding multiple attractors, however, has a rigorous foundation only for closed, thermodynamically non-driven, chemical systems, such as a protein. Recent advances in the theory of nonlinear stochastic dynamical systems and its applications to mesoscopic reaction networks, one reaction at a time, have provided a new basis for a landscape of open, driven biochemical reaction systems under sustained chemostat. The theory is equally applicable not only to …

Draft Nuclear Genome Sequence Of The Liquid Hydrocarbon–Accumulating Green Microalga Botryococcus Braunii Race B (Showa), Daniel R. Browne, Jerry Jenkins, Jeremy Schmutz, Shengqiang Shu, Kerrie Barry, Jane Grimwood, Jennifer Chiniquy, Aditi Sharma, Thomas Daniel Niehaus, Taylor L. Weiss, Andrew T. Koppisch, David T. Fox, Suraj Dhungana, Shigeru Okada, Joe Chappell, Timothy P. Devarenne

Plant and Soil Sciences Faculty Publications

Botryococcus braunii has long been known as a prodigious producer of liquid hydrocarbon oils that can be converted into combustion engine fuels. This draft genome for the B race of B. braunii will allow researchers to unravel important hydrocarbon biosynthetic pathways and identify possible regulatory networks controlling this unusual metabolism.