Digital Commons Network^™

Phosphorylation Of Cdk2 At Threonine 39 By Akt Facilitates Cyclin-Cdk2 Activation, Thiago Bezerra Gaspar Carvalho Da Silva

Open Access Dissertations

Cdk2 importantly regulates G1 progression. Cdk2 activation requires cyclin binding and phosphorylation at T160 by CAK. Here we describe a novel Cdk2 site whose Akt dependent phosphorylation appears to facilitate cyclin-Cdk2 assembly Cdk2 bears a Akt consensus motif containing threonine 39 (T39) immediately preceding the PSTAIRE helix. Cellular Cdk2 co-precipitated with Akt and Akt phosphorylated Cdk2 in vitro. Treatment of quiescent cells with serum leads to activation of the Akt pathway, followed by phosphorylation of Cdk2T39. This phosphorylation preceded the formation of cyclin-Cdk2 complexes and the phosphorylation at Cdk2T160. PI3K inhibition caused cyclin E dissociation from Cdk2, loss of CAK …

In The Middle, Nicole Pugh

University of New Orleans Theses and Dissertations

A woman just getting settled in New Orleans with her fiancé is uprooted by Hurricane Katrina. She spends the two months after the hurricane in various parts of Louisiana trying to pick up the pieces of her uprooted reality. Along the way, she encounters ordinary people who act as inspirations and is also reminded of her deceased Chinese grandmother, whom she was care-giver to before she died and whose stories about life in China and the US parallel the woman´s own life during the post-Katrina months of vulnerability and change.

Does Presentation Make A Difference To Risk Perception: Testing Different Formats For Communication Of Cancer Risks, Sandra C. Jones

Sandra Jones

Evidence suggests that the presentation format of risk information can affect people’s perceptions of risk and influence health-related decisions. In these studies we investigated the impact of four different risk presentation formats: standard presentation, risk ladder, different base rates and visual representations on women’s perceptions of developing breast cancer of lymphoma. We found that the different presentations had virtually no impact on the participant’s risk estimates. Only in the second study relating to risk perceptions for lymphoma was there a significant difference between conditions for estimated 10-year-risk, with those in the ladder present condition reporting a lower estimated risk. The …

Structural Insights Into Dna Replication And Lesion Bypass By Y Family Dna Polymerases, Kevin N. Kirouac

Electronic Thesis and Dissertation Repository

Y family DNA polymerases are specialized enzymes for replication through sites of DNA damage in the genome. Although the DNA damage bypass activity of these enzymes is important for genome maintenance and integrity, it is also responsible for DNA mutagenesis due to the error-prone nature of the Y family. Understanding how these enzymes select incoming nucleotides during DNA replication will give insight into their role in cancer formation, aging, and evolution. This work attempts to mechanistically explain, primarily through X-ray crystallography and enzymatic activity assays, how Y family polymerases select incoming nucleotides in various DNA replication contexts. Initially, we sought …

Mean Cancer Mortality Rates In Low Versus High Elevation Counties In Texas, John Hart

Dose-Response: An International Journal

There is controversy as to whether low levels of radiation (i.e., < 5 rem) pose a health risk. This brief inquiry compares archived cancer mortality data in counties having relatively low (0-250 feet above sea level), medium (500-1000 feet above sea level), and high (3000+ feet above sea level) elevations also having corresponding greater natural back- ground levels of radiation respectively. Cancer mortality was found to be lowest in the high elevation counties (mean = 58.2) followed by low elevation counties (67.5) and then medium elevation counties (70.4). Statistically significant differences were found between low –high elevations (p = 0.003), and medium – high elevations (p = 0.010), but not between low and medium elevations (p = 0.5). More rigorous research, with an accounting of con- founding variables, is indicated.

Obesity And Acute Lymphocytic Leukemia, Jacob Michael Taylor

Electronic Theses and Dissertations

Obesity rates for pediatric acute lymphocytic leukemia (ALL) survivors vary from 11%-57%. The purpose of this study was to evaluate the relationship between caloric and macronutrient intake on the incidence of obesity in survivors of pediatric ALL. A retrospective study of 137 participants using existing data collected from the Bone II study was evaluated. Participants were grouped into categories based on BMI for adults and CDC growth charts for children. Data was collected from 24 hour food recalls at time of enrollment. No statistical significance was found between BMI groups. Forty percent of participants were overweight or obese, but 69% …

Poeta Power: The Poetic Journey Of La Erika: Poems, Erika Marie Garza-Johnson

Theses and Dissertations - UTB/UTPA

This is a collection of poetry set in the borderlands of deep South Texas. The poems take as their subject Chican@ identity, family, the Rio Grande Valley of South Texas, Edcouch-Elsa, Texas, cancer, sexuality, Chicana feminism, childbirth and children, marriage, education, folklore, epithets, among others. As a cycle, they represent the poet‘s development through key stages in her life, including childbirth, marriage, and death of a parent. Many poems in this collection also reflect the linguistic diversity of the U.S.-Mexico border through the poet’s use of code-switching and Tex Mex.

Antimicrobial And Anticancer Activity Of Essential Oils From Guatemalan Medicinal Plants, Andrew B. Miller

Theses and Dissertations

Guatemalan medicinal plants were collected and screened for the presence of essential oils using steam distillation. Oil was found in 63 species from 24 families and was tested in tube dilution assays for activity against Escherichia coli, Staphylococcus aureus, Streptococcus mutans, Lactobacillus acidophilus and Candida albicans. Several essential oils were highly active with 20 instances of oils inhibiting the microbes at an MIC of 0.31 µl/ml. Oils were also tested against cancerous and established cell lines using a 15% (v/v) agar-media which was developed to improve essential oil solubility. Assays were performed against three cancer lines: …

The Heroes' Journey: A Young Couple's Experience With Choriocarcinoma, Dan Marlowe, Jennifer Hodgson, Angela Lamson

The Qualitative Report

A 20 year retrospective qualitative case study was conducted to investigate the relational impact of choriocarcinoma (a type of gestational cancer) on a couple of child-bearing age. A unique feature to the study was that the primary investigator was the couple's biological son, initiating the first known auto-case study design. Using holistic content analysis, investigators learned that anticipatory grief played a central part in the couple's ultimate detachment and divorce. While the wife began to refocus her emotional energies on her children, in psychological preparation for her possible death, the husband focused on ways to keep the family finances together …

Knowledge, Attitudes, And Practice Behaviors Of Oncology Advanced Practice Nurses Regarding Advanced Care Planning For Patients With Cancer., Guiyun Zhou, Dnp, Crnp, Aocns, Jill C Stoltzfus, Phd, Arlene D Houldin, Phd, Pmhcns-Bc, Susan M Parks, Md, Beth Ann Swan, Phd, Crnp, Faan

College of Nursing Faculty Papers & Presentations

Purpose/Objectives: To establish initial reliability and validity of a Web-based survey focused on oncology advanced practice nurses' (APNs') knowledge, attitudes, and practice behaviors regarding advanced care planning, and to obtain preliminary understanding of APNs' knowledge, attitudes, and practice behaviors and perceived barriers to advanced care planning.

Design: Descriptive, cross-sectional, pilot survey study.

Setting: The eastern United States.Sample: 300 oncology APNs.Methods: Guided by the Theory of Planned Behavior, a knowledge, attitudes, and practice behaviors survey was developed and reviewed for content validity. The survey was distributed to 300 APNs via e-mail and sent again to the 89 APNs who responded to …

What Are The Factors That Predict Outcome At Relapse After Previous Esophagectomy And Adjuvant Therapy In High-Risk Esophageal Cancer?, Edward Yu, P. Tai, R. Malthaner, L. Stitt, G. Rodrigues, R. Dar, B. Yaremko, J. Younus, M. Sanatani, M. Vincent, B. Dingle, D. Fortin, R. Inculet

Edward Yu

OBJECTIVES: The present study investigated factors affecting outcome at relapse after previous surgery and adjuvant chemoradiation (crt) in high-risk esophageal cancer patients. PATIENTS AND METHODS: From 1989 to 1999, we followed high-risk resected esophageal cancer patients who had completed postoperative crt therapy. Patients who relapsed with a disease-free interval of less than 3 months were treated with palliative crt when appropriate. Patients with a disease-free interval of 3 months or more were treated with best supportive care. Post-recurrence survival was estimated using the Kaplan-Meier technique, and statistical comparisons were made using log-rank chi-square tests and Cox regression. RESULTS: Of the …

Taylor: A Magazine For Taylor University Alumni, Parents And Friends (Fall 2010), Taylor University

The Taylor Magazine (1963-Present)

The Fall 2010 edition of Taylor Magazine, published by Taylor University in Upland, Indiana.

Proteoglycans In Health And Disease: Novel Regulatory Signaling Mechanisms Evoked By The Small Leucine-Rich Proteoglycans., Renato V. Iozzo, Liliana Schaefer

Department of Pathology, Anatomy, and Cell Biology Faculty Papers

The small leucine-rich proteoglycans (SLRPs) are involved in many aspects of mammalian biology, both in health and disease. They are now being recognized as key signaling molecules with an expanding repertoire of molecular interactions affecting not only growth factors, but also various receptors involved in controlling cell growth, morphogenesis and immunity. The complexity of SLRP signaling and the multitude of affected signaling pathways can be reconciled with a hierarchical affinity-based interaction of various SLRPs in a cell- and tissue-specific context. Here, we review this interacting network, describe new relationships of the SLRPs with tyrosine kinase and Toll-like receptors and critically …

Orally Active Mmp-1 Sparing Α-Tetrahydropyranyl And Α-Piperidinyl Sulfone Matrix Metalloproteinase (Mmp) Inhibitors With Efficacy In Cancer, Arthritis, And Cardiovascular Disease, Daniel P. Becker, Thomas E. Barta, Louis J. Bedell, Terri L. Boehm

Chemistry: Faculty Publications and Other Works

α-Sulfone-α-piperidine and α-tetrahydropyranyl hydroxamates were explored that are potent inhibitors of MMP’s-2, -9, and -13 that spare MMP-1, with oral efficacy in inhibiting tumor growth in mice and left-ventricular hypertrophy in rats and in the bovine cartilage degradation ex vivo explant system. α-Piperidine 19v (SC-78080/SD-2590) was selected for development toward the initial indication of cancer, while α-piperidine and α-tetrahydropyranyl hydroxamates 19w (SC-77964) and 9i (SC-77774), respectively, were identified as backup compounds.

Antifolate Modulators Of Amp-Activated Protein Kinase Signaling As Cancer Therapeutics, Scott Rothbart

Theses and Dissertations

Since its discovery, it was appreciated that the antifolate pemetrexed had multiple targets within folate metabolism. This laboratory was instrumental in showing that pemetrexed elicited its primary action as a thymidylate synthase inhibitor. Unusual for an antifolate, pemetrexed showed significant clinical activity against malignant pleural mesothelioma and non-small cell lung cancer. Accordingly, the FDA recently issued first-line approvals for pemetrexed in these diseases, leading us to question whether the effects of pemetrexed on other folate-dependent targets could explain this atypical clinical activity of the drug. Studies in this dissertation showed that in addition to thymidylate synthase inhibition, pemetrexed was also …

The Benefits Of Contaminated Site Cleanup Revisited: The Case Of Naples And Caserta, Italy, Anna Alberini, Milan Ščasný, Dennis Guignet, Stefania Tonin

Fondazione Eni Enrico Mattei Working Papers

Guerriero and Cairns (2009) recently estimate that contaminated sites and improper waste management result in 848 excess deaths per year in the provinces of Naples and Caserta in Southern Italy, 403 of which are fatal cancers. In the absence of estimates of the Value of a Prevented Fatality (VPF) in Italy or specific to the hazardous waste context, they use figures recommended by DG-Environment. Contrary to their claims, estimates of the VPF are available for Italy that are specific to the hazardous waste context, and for causes of death that have been linked to contaminated site exposures. We review them …

Association Between Local External Gamma Rays And Frequency Of Cancer In Babol-Iran, Ali Shabestani Monfared, Karimollah Hajian, Reza Hosseini, Akbar Nasir

Dose-Response: An International Journal

Introduction: The effect of natural background radiation on Cancer is still challenging. The investigation of association between external gamma rays and Cancer was the main goal of study. Materials & Methods: External Gamma rays were measured using a radiation survey meter in 184 urban and rural health centers to estimate the exposure to the population in residential areas of Babol. The dose distribution map was compared to the 5 years radiation induced cancer incidence data from cancer registry center in north part of Iran. Results: Results showed that although the external gamma ray level in Babol is nearly equal to …

Kinetics Of Dna And Rna Hybridization In Serum And Serum-Sds, Elton Graugnard, Amber Cox, Jeunghoon Lee, Cheryl Jorcyk, Bernard Yurke, William L. Hughes

Materials Science and Engineering Faculty Publications and Presentations

Cancer is recognized as a serious health challenge both in the United States and throughout the world. While early detection and diagnosis of cancer leads to decreased mortality rates, current screening methods require significant time and costly equipment. Recently, increased levels of certain micro-ribonucleic acids (miRNAs) in the blood have been linked to the presence of cancer. While blood-based biomarkers have been used for years in cancer detection, studies analyzing trace amounts of miRNAs in blood and serum samples are just the beginning. Recent developments in deoxyribonucleic acid (DNA) nanotechnology and DNA computing have shown that it is possible to …

Wow! What A Ride! A Journey With Cancer (Book Review), Pam Adams

Pro Rege

Reviewed Title: Bosma, Cella. Wow! What a Ride! A Journey with Cancer. Sioux Center, IA: Dordt College Press, 2010. 274 pages. ISBN: 978-0-932914-84-2.

Synthesis Of A Water Soluble Resveratrol Derivative As A Potential Anti-Cancer Drug., Augustine Essel

Electronic Theses and Dissertations

Research on development of water soluble anti-cancer drugs is one of the great challenges of modern medicinal chemistry. Resveratrol (Res) is one of the many phytoalexins producing stilbenoids present in several medicinal plants, grape skin, peanuts, and red wine. It has been found to exhibit anti-cancer, anti-inflammatory, and anti-oxidant properties. Water solubility and bioavailability are some of the setbacks of this interesting compound. In view of this, effort has been made to synthesize amino acid derivative of resveratrol to improve its bioavailability and solubility in water. Methyl 4-{-[(1E)-2-(3, 5-dihydroxyphenyl)-ethenyl] - phenoxy} butyrate (7), a novel ester intermediate, has been synthesized …

Fueling The Coal War--The Courts, The Feds, And The Epa: Who Is In A Better Position To Curb Coal-Related Pollution?, Corwyn Davis

Corwyn M Davis

ABSTRACT: With the United States’ continued and growing dependence on the use of coal for energy production, it is vital that the country examines ways to eliminate coal wastes more efficiently. The courts have varying opinions on who should ultimately bear responsibility for environmental torts connected with carbon pollution. With greenhouse gases and global warming stealing the environmental spotlight, the equally hazardous nature of coal combustion waste disposal has taken a back door to national policy reform. This paper introduces the problems associated with the disposal of this hazardous by-product. By analyzing the status quo of environmental regulation, it becomes …

Characterizing The Role Of Dna Repair Proteins In Telomere Length Regulation And Maintenance: Fanconi Anemia Complementation Group C Protein And 8-Oxoguanine Dna Glycosylase, David Beomjin Rhee

Doctoral Dissertations

Telomeres are the chromosome end structures consisting of telomere-associated proteins and short tandem repeat sequences, TTAGGG, in humans and mice. Telomeres prevent chromosome termini from being recognized as broken DNA ends. The structural integrity of DNA including telomeres is constantly threatened by a variety of DNA damaging agents on a daily basis. To counteract the constant threats from DNA damage, organisms have developed a number of DNA repair pathways to ensure that the integrity of genome remains intact. A number of DNA repair proteins localize to telomeres and contribute to telomere maintenance; however, it is still unclear as to what …

A Mobile Threat To Genome Stability: The Impact Of Non-Ltr Retrotransposons Upon The Human Genome, Miriam K. Konkel, Mark A. Batzer

Faculty Publications

It is now commonly agreed that the human genome is not the stable entity originally presumed. Deletions, duplications, inversions, and insertions are common, and contribute significantly to genomic structural variations (SVs). Their collective impact generates much of the inter-individual genomic diversity observed among humans. Not only do these variations change the structure of the genome; they may also have functional implications, e.g. altered gene expression. Some SVs have been identified as the cause of genetic disorders, including cancer predisposition. Cancer cells are notorious for their genomic instability, and often show genomic rearrangements at the microscopic and submicroscopic level to which …

Understanding Acquired Resistance To Lapatinib In Breast Cancer Cells, Jen-Te Tseng

Dissertations & Theses (Open Access)

Signaling through epidermal growth factor receptor (EGFR/ErbB) family members plays a very important role in regulating proliferation, development, and malignant transformation of mammary epithelial cells. ErbB family members are often over-expressed in human breast carcinomas. Lapatinib is an ErbB1 and ErbB2 tyrosine kinase inhibitor that has been shown to have anti-proliferative effects in breast and lung cancer cells. Cells treated with Lapatinib undergo G1 phase arrest, followed by apoptosis. Lapatinib has been approved for clinical use, though patients have developed resistance to the drug, as seen previously with other EGFR inhibitors. Moreover, the therapeutic efficacy varies significantly within the patient …

A Case Study On The Use Of Athletic Performance Strategies In An Elite Athlete’S Management Of Pancreatic Cancer, Brooke Elisabeth Harris

Boise State University Theses and Dissertations

In 2007, 11.7 million people in the United States were living with a cancer diagnosis. Specifically, 12 in every 100,000 Americans are diagnosed with pancreatic cancer each year. The five year survival rate of pancreatic cancer in the United States is only 5.6% (National Cancer Institute, 2007). In addition to traditional pharmacological treatments, physical activity has been increasingly used to help individuals successfully cope with cancer. One area that has not been studied extensively is the use of sport psychology techniques to help athletes cope with cancer. It is possible that these techniques, learned by elite athletes during their careers, …

Metabolic Syndrome And Risk Of Death From Cancers Of The Digestive System, Charles E. Matthews, Xuemei Sui, Michael J. Lamonte, Swann A. Adams, James R. Hébert, Steven N. Blair

Faculty Publications

We tested the hypothesis that risk of early mortality from cancers of the digestive system will be greater in men with, compared to men without, the metabolic syndrome (MetS). Participants were 33,230 men who were seen at the Cooper Clinic in Dallas, Texas and followed for 14.4 (SD=7.0) yrs. MetS was defined as having at least three of the following risk factors: abdominal obesity, fasting hypertriglyceridemia, low high-density lipoprotein cholesterol, high blood pressure, or high fasting glucose level or diabetes. MetS was associated with higher mortality (HR=1.90 [95% Confidence Interval=1.42-2.55]), and there was a graded positive association for the addition …

Sa-4-1bbl As A Modulator Of T Effector To T Regulatory Cell Conversion., Shravan Madireddi 1980-

Electronic Theses and Dissertations

The immune system is of profound importance for host survival due to its ability to clear many acquired diseases. In case of infections and cancer, disease arises due to under-performance of immune system. Whereas in autoimmunity and transplantation, deleterious immunity to normal self-antigens and allogeneic antigens arises due to enhanced immune responses. Regulatory T (Treg) cells play a critical role in immune homeostasis in health and disease. Treg cells are important in the development and maintenance of immune tolerance towards self or transplantation antigens. However, Treg cells also play an obstructive role in cancer-related immunity by suppressing various effector cells. …

Cell Death And Sustained Senescence Arrest In Colon Carcinoma And Melanoma Tumor Cells In Response To The Novel Microtubule Poison, Jg-03-14, Jonathan Biggers

Theses and Dissertations

Previous studies from this and other laboratories have shown that the novel microtubule poison, JG-03-14, which binds to the colchicine binding site of tubulin, has the capacity to promote both autophagy and apoptosis in breast tumor cells, as well as interfering with endothelial cell function and potentially disrupting tumor vasculature. The current work was designed to investigate the interaction between JG-03-14 and cell culture models of colon carcinoma and melanoma, specifically HCT116 human colon carcinoma cells and B16F10 murine melanoma cells. In both cases, JG-03-14 promoted death in the bulk of the treated population. FACS analysis, DAPI and TUNEL staining …

A Comparative Study Of Knowledge Of Pain Management In Certified And Non-Certified Oncology Nurses, Sherrie A. Lalande

USF Tampa Graduate Theses and Dissertations

Over 1.4 million people are diagnosed with cancer annually. Of those people, 70-90% experience some form of pain. Numerous efforts have been made to educate nurses in the management of pain, yet 30-50% of cancer patients report that their pain is poorly managed. It is not clear whether nurses who obtain certification in Oncology are better equipped to manage this patient population regarding their pain issues. This study compared the knowledge of Oncology Certified Nurses (OCN) and Non-Certified Oncology Nurses (NCON) regarding pain management.

The sample of 41 oncology nurses included 19 who were certified in oncology and 22 who …

Effect Of Nanoparticle Surface Charge At The Plasma Membrane And Beyond, Rr Arvizo, Or Miranda, Ma Thompson, Cm Pabelick, R Bhattacharya, Jd Robertson, Vm Rotello, Ys Prakash, P Mukherjee

Vincent Rotello

Herein, we demonstrate that the surface charge of gold nanoparticles (AuNPs) plays a critical role in modulating membrane potential of different malignant and nonmalignant cell types and subsequent downstream intracellular events. The findings presented here describe a novel mechanism for cell-nanoparticle interactions and AuNP uptake: modulation of membrane potential and its effect on intracellular events. These studies will help understand the biology of cell-nanoparticle interactions and facilitate the engineering of nanoparticles for specific intracellular targets.