Digital Commons Network^™

The Effects Of Age And Heterochromatin On Frequencies Of Acquired Chromosomal Aneuploidy In Uncultured Human Leukocytes, Noran Aboalela

Theses and Dissertations

While age-related sex chromosomal aneuploidy is a well-characterized phenomenon, the relationship between autosomal loss and age remains unclear. The emergence of the specific and highly sensitive fluorescence in situ hybridization (FISH) technology has enabled investigators to study interphase cells, thereby overcoming problems inherent with the study of metaphase spreads for acquired aneuploidy assessment. Despite all the advantages of this technique, there are some limitations that could be misleading when scoring interphase autosomal aneuploidy. In this study we show that sex chromosomal hypoploidy is correlated with age. By using a twin study design, we evaluated Y chromosome hypoploidy frequencies and found …

Complex Decision Support For Older Adults: Effects Of Information Visualization On Decision Performance, Margaux Price

All Theses

Older adults are faced with complex decision tasks that impose high working memory demands. A representative task is choosing a prescription drug plan from a multitude of options that must be evaluated along many factors. The combined effect of the quantity of complex information, and reduced working memory capacity puts older adults at a disadvantage. However, research with younger adults suggests that the working memory burden of decision tasks can be reduced using well-designed, graphical decision aids (i.e., environmental supports). The current study examined the use of environmental supports to support complex decision-making for older adults. Two experiments were conducted; …

Anomalous Loss Of Toughness Of Work Toughened Polycarbonate, Shawn E. Meagher

Department of Engineering Mechanics: Dissertations, Theses, and Student Research

Glassy polymers such as polycarbonate (PC) can be toughened through compressive plastic deformation. The increase in toughness is substantial, showing as much as a fifteen fold increase in the amount of dissipated energy during failure for samples compressed to 50% plastic strain. This toughness increase can be reversed through thermal aging at temperatures below the glass transition temperature (T_g = 147°C).

The combined effect of plastic compression and thermal aging has been studied using Charpy, Single Edge Notch Bending (SENB), and Compact Tension (CT) tests. The tests mapped the response of samples cut along different orientations relative to the …

Borders And Barriers: Perspectives On Aging And Alternative Medicine Among Transnational North Indian Immigrants, Kanan B. Mehta

Anthropology Theses

This study explores the practice of alternative medicine among a group of senior, transnational Indian immigrants. I analyze how cross-cultural ideologies influence aging and immigrant experiences in healthcare. I explore the ways in which transnational networks nurture social relations and aid in acquiring healthcare resources. This study also examines the developments that alternative medicine underwent during the colonial rule and how those developments affected the trajectory of biomedicine. I focus on the practice of alternative medicine as a significant contributor to immigrant health. Finally, I argue that we need to strive for a symbiosis between alternative medicine and Western biomedicine …

Changes In The Proliferation Of The Subventricular Zone Neural Stem Cell Pool Throughout Aging In The Murine Brain, Olayinka Edwards

Honors Scholar Theses

Previous studies regarding subventricular zone (SVZ) neural stem cells (NSCs) have either observed global changes in the SVZ niche containing NSCs or an impure NSC population. By imaging wholemount preparations of mouse brains and staining with molecular markers specific for proliferating NSC nuclei, the study was able to give a more accurate depiction of the SVZ NSC population than its predecessors. This study will quantify the percentage of proliferating NSCs at various ages of adult murine brain development in order to elucidate a possible correlation between NSC division kinetics and the declining neurogenic output witnessed with aging.

Impact Of Aging On Morphine Analgesia And Associated Changes In Μ-Opioid Receptor Binding And Expression In The Ventrolateral Periaqueductal Gray, Richard L. Hanberry Iv

Biology Theses

Chronic pain in the aged is a widespread phenomenon, and morphine is the most commonly used narcotic analgesic for treatment. Despite that fact, there are relatively few published studies examining the impact of advanced age on morphine analgesia. We hypothesized that aged rats would be less sensitive to morphine than adults, and that aged animals would have reduced mu-opioid receptor (MOR) binding and expression in the ventrolateral periaqueductal gray, a brain region responsible for morphine analgesia. Using a model of persistent inflammatory pain, we found that morphine was significantly less effective in aged males compared to adult males, and that …

Implications Of Lifecourse Epidemiology For Research On Determinants Of Adult Disease, Sze Yan Liu, R N. Jones, M Maria Glymour

Department of Public Health Scholarship and Creative Works

Many diseases commonly associated with aging are now thought to have social and physiologic antecedents in early life. Understanding how the timing of exposure to early life risk factors influences later-life health may illuminate mechanisms driving adult health inequalities and identify possible points for effective interventions. Recognizing chronic diseases as developing across the lifecourse also has implications for the conduct of research on adult risk factors for disease. We review alternative conceptual models that describe how the timing of risk factor exposure relates to the development of disease. We propose some expansions of lifecourse models to improve their relevance for …

Implications Of Lifecourse Epidemiology For Research On Determinants Of Adult Disease, Sze Yan Liu, Richard N. Jones, M. Maria Glamour

Department of Public Health Scholarship and Creative Works

Many diseases commonly associated with aging are now thought to have social and physiologic antecedents in early life. Understanding how the timing of exposure to early life risk factors influences later-life health may illuminate mechanisms driving adult health inequalities and identify possible points for effective interventions. Recognizing chronic diseases as developing across the life course also has implications for the conduct of research on adult risk factors for disease. We review alternative conceptual models that describe how the timing of risk factor exposure relates to the development of disease. We propose some expansions of lifecourse models to improve their relevance …

Workplace Harassment: Conceptualizations Of Older Workers, Amy Blackstone

University of Maine Office of Research Administration: Grant Reports

Drawing from theories and empirical work on gender in the workplace, aging, and sociolegal studies, this study of workplace harassment will assess how the power that older workers hold across a variety of domains including work, family, and community life shapes their harassment experiences and responses to those experiences. The study involves collecting and analyzing survey data on the workplace harassment experiences of 800 Maine workers aged 62 and above. These results will then be used to create a generalized theoretical model which outlines how age and other dimensions of power operate together to shape victimization and mobilization experiences. Four …

Morning Rain, Jason Sturner

The Prairie Light Review

No abstract provided.

Prenatal Undernutrition And Cognitive Function In Late Adulthood, Susanne R. De Rooij, Hans Wouters, Julie E. Yonker, Rebecca C. Painter

University Faculty Publications and Creative Works

At the end of World War II, a severe 5-mo famine struck the cities in the western part of The Netherlands. At its peak, the rations dropped to as low as 400 calories per day. In 1972, cognitive performance in 19-y-old male conscripts was reported not to have been affected by exposure to the famine before birth. In the present study, we show that cognitive function in later life does seem affected by prenatal undernutrition. We found that at age 56 to 59, men and women exposed to famine during the early stage of gestation performed worse on a selective …

Prenatal Undernutrition And Cognitive Function In Late Adulthood, Susanne R. De Rooij, Hans Wouters, Julie E. Yonker, Rebecca C. Painter

University Faculty Publications and Creative Works

At the end of World War II, a severe 5-mo famine struck the cities in the western part of The Netherlands. At its peak, the rations dropped to as low as 400 calories per day. In 1972, cognitive performance in 19-y-old male conscripts was reported not to have been affected by exposure to the famine before birth. In the present study, we show that cognitive function in later life does seem affected by prenatal undernutrition. We found that at age 56 to 59, men and women exposed to famine during the early stage of gestation performed worse on a selective …

Legal Needs Assessment Of Older Adults In Maine, Lenard Kaye, Melissa Adle, Jennifer Crittenden

Maine Center on Aging Research and Evaluation

A clear understanding of the most pertinent legal issues for older adults is necessary to maximize the available resources and increase the capacity of the legal service delivery system most effectively. This legal needs assessment was conducted by analyzing recent research in six other states over a one year time period. The assessment analyzes more than 7,300 older adults in these six states. It was concluded that up to half of all older adults will need legal assistance in the next five to ten years, but LSE can serve less than five thousand people per year. The greatest need was …

Mip/Mtmr14 And Muscle Aging, Scott K. Powers, Michael B. Reid

Physiology Faculty Publications

No abstract.

Prediction Of Cognitive Decline In Healthy Older Adults Using Fmri, John L. Woodard, Michael Seidenberg, Kristy A. Nielson, J Carson Smith, Piero Antuono, Sally Durgerian, Leslie Guidotti, Qi Zhang, Alissa Butts, Nathan Hantke, Melissa A. Lancaster, Stephen M. Rao

Psychology Faculty Research and Publications

Few studies have examined the extent to which structural and functional MRI, alone and in combination with genetic biomarkers, can predict future cognitive decline in asymptomatic elders. This prospective study evaluated individual and combined contributions of demographic information, genetic risk, hippocampal volume, and fMRI activation for predicting cognitive decline after an 18-month retest interval. Standardized neuropsychological testing, an fMRI semantic memory task (famous name discrimination), and structural MRI (sMRI) were performed on 78 healthy elders (73% female; mean age = 73 years, range = 65 to 88 years). Positive family history of dementia and presence of one or both apolipoprotein …

Plasticity Of Brain Networks In A Randomized Intervention Trial Of Exercise Training In Older Adults, Michelle W. Voss, Ruchika S. Prakash, Kirk I. Erickson, Chandramallika Basak, Laura Chaddock, Jennifer S. Kim, Heloisa Alves, Susie Heo, Amanda N. Szabo, Siobhan M. White, Thomas R. Wójcicki, Emily L. Mailey, Neha Gothe, Erin A. Olson, Edward Mcauley, Arthur F. Kramer

Kinesiology, Health and Sport Studies

Research has shown the human brain is organized into separable functional networks during rest and varied states of cognition, and that aging is associated with specific network dysfunctions. The present study used functional magnetic resonance imaging (fMRI) to examine low-frequency (0.008 < f < 0.08 Hz) coherence of cognitively relevant and sensory brain networks in older adults who participated in a 1-year intervention trial, comparing the effects of aerobic and nonaerobic fitness training on brain function and cognition. Results showed that aerobic training improved the aging brain’s resting functional efficiency in higher-level cognitive networks. One year of walking increased functional connectivity between aspects of the frontal, posterior, and temporal cortices within the Default Mode Network and a Frontal Executive Network, two brain networks central to brain dysfunction in aging. Length of training was also an important factor. Effects in favor of the walking group were observed only after 12 months of training, compared to non-significant trends after 6 months. A non-aerobic stretching and toning group also showed increased functional connectivity in the DMN after 6 months and in a Frontal Parietal Network after 12 months, possibly reflecting experience-dependent plasticity. Finally, we found that changes in functional connectivity were behaviorally relevant. Increased functional connectivity was associated with greater improvement in executive function. Therefore the study provides the first evidence for exercise-induced functional plasticity in large-scale brain systems in the aging brain, using functional connectivity techniques, and offers new insight into the role of aerobic fitness in attenuating age-related brain dysfunction.

Age-Related Deterioration Of Rod Vision In Mice, Alexander V. Kolesnikov, Jie Fan, Rosalie K. Crouch, Vladimir J. Kefalov

Open Access Publications

Even in healthy individuals, aging leads to deterioration in visual acuity, contrast sensitivity, visual field, and dark adaptation. Little is known about the neural mechanisms that drive the age-related changes of the retina and, more specifically, photoreceptors. According to one hypothesis, the age-related deterioration in rod function is due to the limited availability of 11-cis-retinal for rod pigment formation. To determine how aging affects rod photoreceptors and to test the retinoid-deficiency hypothesis, we compared the morphological and functional properties of rods of adult and aged B6D2F1/J mice. We found that the number of rods and the length of their outer …

Accuracy Of Estimating Age And Antler Size Of Photographed Deer, Jeremy J. Flinn

Theses and Dissertations

Objective and accurate techniques are needed to estimate age and antler size of live white-tailed deer (Odocoileus virginianus), because these parameters are essential to many white-tailed deer management strategies. I developed and evaluated accuracy of methods for estimating age and antler size from photographs of live, male white-tailed deer using Geographic Information Systems (GIS). I estimated size of photographed, known-score mounted antlers accurately using a fixed-scale object and photographed, live deer using anatomical features. I determined if a series of morphometric ratios could be used to predict age of deer from photographs using a dichotomous key procedure. Mean percentage error …

Biomarkers For Aging, Philip W. Landfield, Eric M. Blalock, Kuey-Chu Chen, Thomas S. Foster

Pharmacology and Nutritional Sciences Faculty Patents

A statistical and functional correlation strategy to identify changes in cellular pathways specifically linked to impaired cognitive function with aging. Analyses using the strategy identified multiple groups of genes expressed in the hippocampi of mammals, where the genes were expressed at different levels for several ages. The aging changes in expression began before mid-life. Many of the genes were involved in specific neuronal and glial pathways with previously unrecognized relationships to aging and/or cognitive decline. These identified genes and the proteins they encode can be used as novel biomarkers of brain aging and as targets for developing treatment methods against …

An Examination Of Caregiving Dyads: Community Dwelling Chronically Ill Older Adults And Their Caregivers, Jessica E. Haxton

Electronic Theses and Dissertations

Increases in life expectancy among older adults raise important concerns about the availability of resources for an aging population living with chronic and debilitating illnesses. Living longer is complicated by the fact that many elders prefer to reside in their homes until medical or other conditions require an alternative living arrangement. The strong desire to remain at home expressed by older persons in the United States has in turn created an increased demand on informal caregiving. Consequently, adult children often bear the burden of providing care to their aging parents. In view of this demand it is critical that research …

Scoping Study Of The Effects Of Aging On Landmines Phase 2, Cisr Jmu

CISR Studies and Reports

Most of the mines that currently threaten populations were manufactured more than 50 years ago and many have been in the ground for 30 years or more. Despite the inevitable and obvious deterioration, there has been very little research into the effects of aging on landmines. In 2008, James Madison University (JMU), the Center for International Stabilization and Recovery (CISR), and C King Associates Ltd (CKA) began a study designed to understand the aging process and the range of implications for the various components of mine action. The two-and-a-half year study was funded by grants from the US Department of …

Study Of The Effects Of Aging On Landmines, Daniele Ressler

CISR Studies and Reports

Most of the mines that currently threaten populations were manufactured more than 50 years ago and many have been in the ground for 30 years or more. Despite the inevitable and obvious deterioration, there has been very little research into the effects of aging on landmines.

In 2008, James Madison University (JMU), the Center for International Stabilization and Recovery (CISR), and C King Associates Ltd (CKA) began a study designed to understand the aging process and the range of implications for the various components of mine action. The two-and-a-half year study was funded by grants from the US Department of …

Promoting Older Adults' Health Through Policy, Pamela L. Buckmaster

Public Health Theses

The purpose of this capstone project was to develop the content for an online training module entitled Promoting Older Adults’ Health through Policy. The Centers for Disease Control and Prevention (CDC) Aging and Health Work Group was interested in complementing their workshop, Promoting Older Adults’ Health: Opportunities and Resources for CDC Professionals with an online training module on aging and policy.

This project highlights significant pieces of U.S. legislation that promotes older adults’ health and draws attention to emerging policy, systems, and environmental changes on the horizon. An anticipated short-term outcome is a demonstrated sensitivity to population aging in all …

Alterations In The Cellular Composition Of The Mouse Bladder Following Ovariectomy, Partial Bladder Outlet Obstruction, And Aging, Diane Holly Smith

Honors Scholar Theses

Detrusor underactivity (DU) increases susceptibility to urinary retention and accordingly further complicates the management of urinary incontinence. Bladder muscle stretch, a lack of estrogen, and aging are 3 notable DU risk factors. The aim of this research is to better characterize the changes in cellular composition of the bladder that result from these 3 risk factors to gain a better understanding of DU pathogenesis and pathobiology. This research focuses on the effects of a lack of estrogen while also providing an outline for determining the effects of bladder muscle stretch and aging on the cellular composition of the bladder.

Dopaminergic Innervation Of The Subventricular Zone In The Murine Brain, Linda Beth Drozdowicz

Honors Scholar Theses

The subventricular zone (SVZ) is one of two areas in the brain that, in a healthy mouse, continually generate neurons throughout adulthood. While it was previously thought that only the A9 neurons of the substantia nigra sent dopaminergic afferents to the SVZ, recent studies suggest that the A10 neurons of the ventral tegmental area may innervate this area. This project has aimed to discover which, if either, model is correct.

Examination of the Aphakia (AK) mouse was used to determine the role of distinct midbrain regions in SVZ regulation. Additionally, intraperitoneal injections of the chemical MPTP were used to deduce …

Aging-Dependent Effects Of Repetitive Loading Exercise And Antioxidant Supplementation On Oxidative Stress In Skeletal Muscle, Michael J. Ryan

Graduate Theses, Dissertations, and Problem Reports

Aging is associated with a reduced ability to buffer oxidants along with an increase in oxidant production, resulting in chronic oxidative stress. Oxidative stress is a putative factor responsible for reducing function ability of skeletal muscle and increasing oxidative damage. The objective of this set of investigations was to evaluate the efficacy of reducing oxidative stress on improving muscle function the adaptive response of skeletal muscle to repetitive loading exercise in aging rodents. To achieve this objective, three methods of reducing oxidative stress were utilized; the antioxidant vitamins E&C were used to buffer oxidants, the nutraceutical resveratrol was used to …

The Effect Of Aging And Resveratrol Supplementation On Bone Mass And Strength In Hindlimb Suspended Male Rats, Stephanie Wright

Graduate Theses, Dissertations, and Problem Reports

Resveratrol has recently become a popular dietary supplement because of its potential for reducing the effects of aging and various age-related diseases. However, few studies have investigated the role of resveratrol in improving bone loss associated with aging. It has been widely reported that mechanical unloading increases bone loss. The elderly experience a prevalence of reduced mechanical loading due to declining activity levels and increased incidence of bed rest. In rats, hind-limb suspension (HLS) simulates mechanical unloading of bone. Therefore, the objective of this study was to examine the effects of aging and resveratrol supplementation on bone health in HLS …

Effects Of Long-Term Pioglitazone Treatment On Peripheral And Central Markers Of Aging, Eric M. Blalock, Jeremiah T. Phelps, Tristano Pancani, James L. Searcy, Katie L. Anderson, John C. Gant, Jelena Popovic, Margarita G. Avdiushko, Don A. Cohen, Kuey-Chu Chen, Nada M. Porter, Olivier Thibault

Pharmacology and Nutritional Sciences Faculty Publications

BACKGROUND: Thiazolidinediones (TZDs) activate peroxisome proliferator-activated receptor gamma (PPARgamma) and are used clinically to help restore peripheral insulin sensitivity in Type 2 diabetes (T2DM). Interestingly, long-term treatment of mouse models of Alzheimer's disease (AD) with TZDs also has been shown to reduce several well-established brain biomarkers of AD including inflammation, oxidative stress and Abeta accumulation. While TZD's actions in AD models help to elucidate the mechanisms underlying their potentially beneficial effects in AD patients, little is known about the functional consequences of TZDs in animal models of normal aging. Because aging is a common risk factor for both AD and …

Insulin-Like Growth Factor Binding Protein-3 (Igfbp-3) Plays An Essential Role In Cellular Senescence: Molecular And Clinical Implications., Amanda Garza

Theses and Dissertations

Normal somatic cells have a limited proliferative capacity in vivo and in vitro, termed senescence and later, thought to contribute to molecular and cellular organismal aging. There are several studies that demonstrate the importance of the GH/IGF axis in longevity, aging and cellular senescence. One primary component of the IGF signaling involves IGFBP-3. It is well documented that IGFBP-3 levels are significantly increased in senescent human diploid fibroblasts however IGFBP-3 function is not known in this system. Interestingly, Werner syndrome fibroblasts, commonly used as a model of cellular aging, have upregulated IGFBP-3 levels in young and late passage cells compared …

Mechanisms Associated With Aging And Age-Related Disease In Drosophila, Melanie Jones

Theses and Dissertations

Aging is an intrinsic process that is independent of obvious disease. In contrast to normal aging, age-related diseases are conditions that typically manifest at advanced ages, are associated with explicit pathology and cause disability and premature death. We used Drosophila as a model to investigate the molecular-genetic mechanisms associated with aging and age-related disease. Age-related locomotor impairment (ARLI) is a serious condition for the elderly and greatly impacts their quality of life. Toward identifying genes and mechanisms that influence ARLI, we performed a forward genetic screen using Drosophila mutants. This screen identified a loss of function mutant in PDK1, a …