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Articles 1 - 24 of 24
Full-Text Articles in Entire DC Network
Treatment Of Solid Organ Transplant Recipients With Autologous Epstein Barr Virus-Specific Cytotoxic T Lymphocytes (Ctls)., Barbara Savoldo, John A. Goss, Markus M. Hammer, Lan Zhang, Teresita Lopez, Adrian P. Gee, Yu-Feng Lin, Ruben E. Quiros-Tejeira, Petra Reinke, Stephan Schubert, Stephen Gottschalk, Milton J. Finegold, Malcolm K. Brenner, Cliona M. Rooney, Helen E. Heslop
Treatment Of Solid Organ Transplant Recipients With Autologous Epstein Barr Virus-Specific Cytotoxic T Lymphocytes (Ctls)., Barbara Savoldo, John A. Goss, Markus M. Hammer, Lan Zhang, Teresita Lopez, Adrian P. Gee, Yu-Feng Lin, Ruben E. Quiros-Tejeira, Petra Reinke, Stephan Schubert, Stephen Gottschalk, Milton J. Finegold, Malcolm K. Brenner, Cliona M. Rooney, Helen E. Heslop
Faculty Publications
We have investigated the in vivo safety, efficacy, and persistence of autologous Epstein Barr virus (EBV)-specific cytotoxic T lymphocytes (CTLs) for the treatment of solid organ transplant (SOT) recipients at high risk for EBV-associated posttransplantation lymphoproliferative disease (PTLD). EBV-CTLs generated from 35 patients expanded with normal kinetics contained both CD8 and CD4 lymphocytes and produced significant specific killing of autologous EBV-transformed B lymphoblastoid cell lines (LCLs). Twelve SOT recipients at high risk for PTLD, or with active disease, received autologous CTL infusions without toxicity. Real-time polymerase chain reaction (PCR) monitoring of EBV-DNA showed a transient increase in plasma EBV-DNA suggestive …
Construction Of A Col11a1 Transgene Vector, Cameron Mckell Beck
Construction Of A Col11a1 Transgene Vector, Cameron Mckell Beck
Theses and Dissertations
Background: Cartilage disorders affect millions of people in the United States alone, with effects ranging from poor skeletal development and joint pain to shortened lifespan and perinatal lethality. Many of these disorders have their root in defects of collagen, type XI collagen being among the most important. A mouse model of such a type XI collagen defect is the chondrodysplasia (cho) mutant. Mice homozygous for this null mutation in the Col11a1 gene do not express the α1 chain of type XI collagen. This results in a functional knockout of type XI collagen, leading to insufficient skeletal development and perinatal lethality. …
Arsenic Exposure Is Associated With Decreased Dna Repair In Vitro And In Individuals Exposed To Drinking Water Arsenic, Angeline S. Andrew, Jefferey L. Burgess, Maria M. Meza, Eugene Demidenko, Mary G. Waugh, Joshua W. Hamilton, Margaret R. Karagas
Arsenic Exposure Is Associated With Decreased Dna Repair In Vitro And In Individuals Exposed To Drinking Water Arsenic, Angeline S. Andrew, Jefferey L. Burgess, Maria M. Meza, Eugene Demidenko, Mary G. Waugh, Joshua W. Hamilton, Margaret R. Karagas
Dartmouth Scholarship
The mechanism(s) by which arsenic exposure contributes to human cancer risk is unknown; however, several indirect cocarcinogenesis mechanisms have been proposed. Many studies support the role of As in altering one or more DNA repair processes. In the present study we used individual-level exposure data and biologic samples to investigate the effects of As exposure on nucleotide excision repair in two study populations, focusing on the excision repair cross-complement 1 (ERCC1) component. We measured drinking water, urinary, or toenail As levels and obtained cryopreserved lymphocytes of a subset of individuals enrolled in epidemiologic studies in New Hampshire (USA) and Sonora …
Recently Integrated Alu Retrotransposons Are Essentially Neutral Residents Of The Human Genome, Richard Cordaux, Jungnam Lee, Liv Dinoso, Mark A. Batzer
Recently Integrated Alu Retrotransposons Are Essentially Neutral Residents Of The Human Genome, Richard Cordaux, Jungnam Lee, Liv Dinoso, Mark A. Batzer
Faculty Publications
Alu elements represent the largest family of human mobile elements in copy number. A controversial issue with implications for both Alu biology and human genome evolution is whether selective pressures are affecting Alu elements on a large scale. To address this issue, we analyzed the genomic distribution of the three youngest known human Alu subfamilies (Ya5a2, Ya8 and Yb9) in conjunction with their insertion polymorphism status in the human population, since selection can only act on polymorphic elements. Our results indicate that: (i) polymorphic and fixed recently integrated Alu elements are found in genomic regions whose GC contents are statistically …
Energy Balance And Breast Cancer Risk: A Prospective Cohort Study, Stephanie A. Navarro Silvera, Jain Meera, Geoffrey R. Howe, Anthony B. Miller, Thomas E. Rohan
Energy Balance And Breast Cancer Risk: A Prospective Cohort Study, Stephanie A. Navarro Silvera, Jain Meera, Geoffrey R. Howe, Anthony B. Miller, Thomas E. Rohan
Department of Public Health Scholarship and Creative Works
While there is evidence that breast cancer risk is positively associated with body mass index (in postmenopausal women) and energy intake and inversely associated with physical activity, few studies have examined breast cancer risk in association with energy balance, the balance between energy intake and expenditure. Therefore, in the cohort study reported here, we studied the independent and combined associations of vigorous physical activity, energy consumption, and body mass index (BMI), with breast cancer risk. The investigation was conducted in 49,613 Canadian women who were participants in the National Breast Screening Study (NBSS) and who completed self- administered lifestyle and …
Modulation Of Fast And Slow Inactivation In Two Cardiac Nav Channel Isoforms By Sdz 211-939, Tyce Jeffrey Kearl
Modulation Of Fast And Slow Inactivation In Two Cardiac Nav Channel Isoforms By Sdz 211-939, Tyce Jeffrey Kearl
Undergraduate Honors Capstone Projects
Here we report a hitherto unknown effect of a synthetic inactivation inhibitor on inactivation in cardiac sodium channels (Nav1.5) from two different species: human and bovine. SDZ 211-93 9 stabilized the slow inactivated-state in both channels as seen by an increased steady-state probability of slow inactivation. SDZ also destabilized the fast-inactivated state and increased the amplitude of persistent currents. SDZ modulated conductance parameters, open-state fast inactivation time constants, and activation kinetics of hNav1.5, but not bNav1.5. These findings will aid future studies designed to elucidate the binding site and molecular mechanisms of inactivation inhibitors …
Paleoepidemiology Of Bacterial Infections Among Prehistoric Human Populations In Northern Chile: An Ancient Dna Approach, Luz-Andrea Pfister
Paleoepidemiology Of Bacterial Infections Among Prehistoric Human Populations In Northern Chile: An Ancient Dna Approach, Luz-Andrea Pfister
UNLV Retrospective Theses & Dissertations
Bacterial pathogens not primarily affecting the skeleton but causing sepsis and death, have not been systematically studied in prehistoric human populations, although increasing evidence support our species long co-evolution with many of them. With molecular methods we can identify bacteria at the species level and distinguish pathogenic from environmental and soil bacteria. Bone marrow of ancient people may provide valuable information about ancient pathogens causing sepsis and death. To test the hypothesis that PCR amplification using universal bacterial primers will identify prehistoric bacterial pathogens in bone marrow, 30 samples were aseptically obtained from partially mummified remains of three archaeological sites …
Effect Of Low Level Laser Therapy On Migration Of Human Skin Cells Across A Standardized Wound, Kathleen Orzechowski
Effect Of Low Level Laser Therapy On Migration Of Human Skin Cells Across A Standardized Wound, Kathleen Orzechowski
UNLV Retrospective Theses & Dissertations
The purpose of this study was to examine the effects that different dosages of low level laser therapy have on human skin cell migration. Normal newborn foreskin fibroblasts were seeded into a four well chamber microscope slide. Cellular monolayers were inflicted with three standardized wounds per well. Following the wounding of cells, each well received low level laser therapy once from a 635 nm laser at one of four treatment dosages (0.0 J/cm 2, 1.0 J/cm2, 1.5 J/cm2, 2.0 J/cm 2) with a power output of 11.5 mW. Digital images were recorded immediately following wounding, and every hour thereafter for …
Application Of Computational Fluid Dynamics For High Energy Efficiency Design With Human Comfort Of Cad-Vav And Ufad Systems, Bhanu Rekha Bandhakavi
Application Of Computational Fluid Dynamics For High Energy Efficiency Design With Human Comfort Of Cad-Vav And Ufad Systems, Bhanu Rekha Bandhakavi
UNLV Retrospective Theses & Dissertations
This thesis deals with the numerical simulation of the ceiling air distribution (CAD) system and the Under Floor Air Distribution (UFAD) system based on the dimensions of BTLab at UNLV. Ceiling Air Distribution (CAD) with variable air volume (VAV) and Under Floor Air Distribution (UFAD) systems have been widely used in different countries. CAD-VAV and UFAD systems designs have been influenced by increasing emphasis on indoor air quality (IAQ), energy conservation, environmental effects, safety, and economics. So, 3-D Computational Fluid Dynamics (CFD) analysis technique was applied to design high energy efficiency and human comfort CAD-VAV and UFAD systems. The goal …
Am I Blue? Depressed Mood And The Consequences Of Self Focus For The Interpretation And Recall Of Ambiguous Words, Paula T. Hertel, L. El-Messidi
Am I Blue? Depressed Mood And The Consequences Of Self Focus For The Interpretation And Recall Of Ambiguous Words, Paula T. Hertel, L. El-Messidi
Psychology Faculty Research
In two experiments, dysphoric and nondysphoric students first concentrated on either self-focused or other-focused phrases and then performed an ostensibly unrelated task involving the interpretation of homographs with both personal and impersonal meanings. In Experiment 1, they constructed sentences for the homographs; dysphoric students' sentences were more emotionally negative (although not more personal) in the self-focused condition than in the other-focused condition. In Experiment 2, they freely associated to the homographs, and the percentage of personal meanings reflected by the associations revealed an effect of self versus other focus that depended on mood group. Following free associations, they attempted to …
A System For The 3d Reconstruction Of The Human Face Using The Structured Light Approach, Dean Mcguire, Prashan Premaratne
A System For The 3d Reconstruction Of The Human Face Using The Structured Light Approach, Dean Mcguire, Prashan Premaratne
Faculty of Informatics - Papers (Archive)
This paper will describe a computer vision system under development with the aim of use in robust facial recognition applications. The system employs the structured light approach to solve the correspondence problem. The system has been designed with ease of use in mind with calibration procedures designed to be simple enough to be carried out without the use of precision measuring equipment, but robust enough to provide good quality 3D reconstructions. The hardware in use is of consumer grade and is being used in an unmodified form. Early reconstructions of faces based on the projection of a striped pattern have …
Working Together For Mental Health: Evaluation Of A One-Day Mental Health Course For Human Service Providers, Pamela E. Grootemaat, Cathie Gillan, Gillian Holt, Wayne Forward, Narelle Heywood, Sue Willis
Working Together For Mental Health: Evaluation Of A One-Day Mental Health Course For Human Service Providers, Pamela E. Grootemaat, Cathie Gillan, Gillian Holt, Wayne Forward, Narelle Heywood, Sue Willis
Sydney Business School - Papers
Background: The Working Together For Mental Health course is an 8-hour course designed to demystify mental illness and mental health services. The main target group for the course is people working in human service organisations who provide services for people with mental illness. Methods: A questionnaire was administered to all participants attending the course during 2003 (n = 165). Participants completed the questionnaire before and immediately after the course, and at three month follow-up. Results: A response rate of 69% was achieved with 114 people completing the questionnaire on all three occasions. The responses showed a significant …
Human Visual Perception Of Region Warping Distortions With Different Display And Scene Characteristics, Yang-Wai Chow, Ronald Pose, Matthew Regan, James Phillips
Human Visual Perception Of Region Warping Distortions With Different Display And Scene Characteristics, Yang-Wai Chow, Ronald Pose, Matthew Regan, James Phillips
Faculty of Engineering and Information Sciences - Papers: Part A
This paper presents human visual perception experiment results for a computer graphics rendering technique introduced in ACSC'05. In order to achieve a good immersive virtual reality experience, it is necessary to have at least 60 frames per second to ensure smooth motion. It is also necessary to have low end-to-end latency so that user interaction does not suffer from perceptible delays in images presented to the eyes. The Address Recalculation Pipeline (ARP) architecture reduces end-to-end latency in immersive Head Mounted Display (HMD) virtual reality systems. By using the ARP in conjunction with priority rendering, different sections of the scene are …
Comparison Of Channelized Hotelling And Human Observers In Determining Optimum Os-Em Reconstruction Parameters For Myocardial Spect, Karen L. Gilland, Benjamin M. W Tsui, Yujin Qi, Grant T. Gullberg
Comparison Of Channelized Hotelling And Human Observers In Determining Optimum Os-Em Reconstruction Parameters For Myocardial Spect, Karen L. Gilland, Benjamin M. W Tsui, Yujin Qi, Grant T. Gullberg
Faculty of Engineering and Information Sciences - Papers: Part A
The performance of the Channelized Hotelling Observer (CHO) was compared to that of human observers for determining optimum parameters for the iterative OS-EM image reconstruction method for the task of defect detection in myocardial SPECT images. The optimum parameters were those that maximized defect detectability in the SPECT images. Low noise, parallel SPECT projection data, with and without an anterior, inferior or lateral LV wall defect, were simulated using the Monte Carlo method. Poisson noise was added to generate noisy realizations. Data were reconstructed using OS-EM at 1 & 4 subsets/iteration and at 1, 3, 5, 7 & 9 iterations. …
In Vitro Cytotoxicity Of Formaldehyde And Glutaraldehyde Mixtures In Human Cells, Shahnaz Bakand, Chris Winder, Christian Khalil, Amanda Hayes
In Vitro Cytotoxicity Of Formaldehyde And Glutaraldehyde Mixtures In Human Cells, Shahnaz Bakand, Chris Winder, Christian Khalil, Amanda Hayes
Faculty of Social Sciences - Papers (Archive)
The cytotoxicity of formaldehyde, glutaraldehyde and their binary mixtures was determined using the MTS (tetrazolium salt; Promega) in vitro assay. Cytotoxicity endpoints were investigated in human cells including; pulmonary type II-like epithelial cell lines (A549), hepatoma cell lines (HepG2) and skin fibroblasts. In order to study the cytotoxic effects of airborne formaldehyde, standard atmospheres at concentrations below 10 ppm (12.3 mg/m3) were generated using a dynamic diffusion method. Formaldehyde was bubbled through serum free culture media and cell viability was investigated after treating cells with formaldehyde air samples. HepG2 cells were found to be more sensitive (IC50 …
Human Visual Perception Of Region Warping Distortions, Yang-Wai Chow, Ronald Pose, Matthew Regan, James Phillips
Human Visual Perception Of Region Warping Distortions, Yang-Wai Chow, Ronald Pose, Matthew Regan, James Phillips
Faculty of Informatics - Papers (Archive)
Interactive virtual reality requires at least 60 frames per second in order to ensure smooth motion. For a good immersive experience, it is also necessary to have low end-to-end latency so that user interaction does not suffer from perceptible delays in images presented to the eyes. The Address Recalculation Pipeline (ARP) architecture reduces end-to-end latency in immersive Head Mounted Display (HMD) virtual reality systems. By using the ARP in conjunction with priority rendering, different sections of the scene are updated at different rates. This reduces the overall rendering load and allows for more complex and realistic scenes. Large object segmentation …
Human Behaviour Recognition With Segmented Inertial Data, Chao Sun, David A. Stirling, Fazel Naghdy
Human Behaviour Recognition With Segmented Inertial Data, Chao Sun, David A. Stirling, Fazel Naghdy
Faculty of Engineering - Papers (Archive)
The development and recent advancements of integrated inertial sensors has afforded substantive new possibilities for the acquisition and study of complex human motor skills and ultimately their imitation within robotic systems. This paper describes continuing work on kinetic models that are derived through unsupervised learning from a continuous stream of signals, including Euler angles and accelerations in three spatial dimensions, acquired from motions of a human arm. An intrinsic classification algorithm, MML (Minimum Message Length encoding) is used to segment the complex data, formulating a Gaussian Mixture Model of the dynamic modes it represents. Subsequent representation and analysis as FSM …
Analysis Of Epstein-Barr Virus Reservoirs In Paired Blood And Breast Cancer Primary Biopsy Specimens By Real Time Pcr., R Serene Perkins, Katherine Sahm, Cindy Marando, Diana Dickson-Witmer, Gregory R Pahnke, Mark Mitchell, Nicholas J Petrelli, Irving M Berkowitz, Patricia Soteropoulos, Virginie M Aris, Stephen P Dunn, Leslie J Krueger
Analysis Of Epstein-Barr Virus Reservoirs In Paired Blood And Breast Cancer Primary Biopsy Specimens By Real Time Pcr., R Serene Perkins, Katherine Sahm, Cindy Marando, Diana Dickson-Witmer, Gregory R Pahnke, Mark Mitchell, Nicholas J Petrelli, Irving M Berkowitz, Patricia Soteropoulos, Virginie M Aris, Stephen P Dunn, Leslie J Krueger
Department of Surgery Faculty Papers
INTRODUCTION: Epstein-Barr virus (EBV) is present in over 90% of the world's population. This infection is considered benign, even though in limited cases EBV is associated with infectious and neoplastic conditions. Over the past decade, the EBV association with breast cancer has been constantly debated. Adding to this clinical and biological uncertainty, different techniques gave contradictory results for the presence of EBV in breast carcinoma specimens. In this study, minor groove binding (MGB)-TaqMan real time PCR was used to detect the presence of EBV DNA in both peripheral blood and tumor samples of selected patients. METHODS: Peripheral blood and breast …
Motivating Rehabilitation By Distorting Reality, James L. Patton, Yejun Wei, Chris Scharver, Robert V. Kenyon, Robert A. Scheidt
Motivating Rehabilitation By Distorting Reality, James L. Patton, Yejun Wei, Chris Scharver, Robert V. Kenyon, Robert A. Scheidt
Biomedical Engineering Faculty Research and Publications
We have found, through a series of recent experiments, encouraging evidence that the neuro-motor system is motivated to change motor patterns when exposed to visuo-motor tasks. We have also shown that the learning of these tasks can be heightened with forces and/or visual distortions that appropriately manipulate the error. This process does not require intense concentration and it is often considered a game. We describe the next generation of robotic large-workspace, three dimensional haptics/graphics systems for rehabilitation
Mutant Neurogenin-3 In Congenital Malabsorptive Diarrhea, Jiafang Wang, Galen Cortina, S. Vincent Wu, Robert Tran, Jang-Hyeon Cho, Ming-Jer Tsai, Travis J. Bailey, Milan Jamrich, Marvin E. Ament, William R. Treem, Ivor D. Hill, Jorge H. Vargas, George Gershman, Douglas G. Farmer, Laurie Reyen, Martin G. Martín
Mutant Neurogenin-3 In Congenital Malabsorptive Diarrhea, Jiafang Wang, Galen Cortina, S. Vincent Wu, Robert Tran, Jang-Hyeon Cho, Ming-Jer Tsai, Travis J. Bailey, Milan Jamrich, Marvin E. Ament, William R. Treem, Ivor D. Hill, Jorge H. Vargas, George Gershman, Douglas G. Farmer, Laurie Reyen, Martin G. Martín
Biology
Background: Neurogenin-3 (NEUROG3) is expressed in endocrine progenitor cells and is required for endocrine-cell development in the pancreas and intestine. The NEUROG3 gene (NEUROG3) is therefore a candidate for the cause of a newly discovered autosomal recessive disorder characterized by generalized malabsorption and a paucity of enteroendocrine cells. Methods: We screened genomic DNA from three unrelated patients with sparse enteroendocrine cells for mutations of NEUROG3. We then tested the ability of the observed mutations to alter NEUROG3 function, using in vitro and in vivo assays. Results: The patients had few intestinal enteroendocrine cells positive for chromogranin A, but they had …
Modification Of Human Immunodeficiency Virus Type 1 Reverse Transcriptase To Target Cells With Elevated Cellular Dntp Concentrations, Varuni K. Jamburuthugoda, Pauline Chugh, Baek Kim
Modification Of Human Immunodeficiency Virus Type 1 Reverse Transcriptase To Target Cells With Elevated Cellular Dntp Concentrations, Varuni K. Jamburuthugoda, Pauline Chugh, Baek Kim
Biology
Retroviruses and DNA viruses utilize cellular dNTPs as substrates for their DNA polymerases during viral replication in infected cells. However, because of S phase-dependent dNTP biosynthesis, the availability of cellular dNTPs significantly varies among cell types (e.g. dividing versus nondividing cells and normal versus tumor cells). Here we tested whether alterations in the dNTP utilization efficiency and dNTP binding affinity of viral DNA polymerases can switch viral infection specificity to cell types with different dNTP concentrations. We employed an HIV-1 reverse transcriptase (RT) mutant (Q151N), which is catalytically active only at high dNTP concentrations because of its reduced dNTP binding …
Characterization Of Hard2, A Processed Hard1 Gene Duplicate, Encoding A Human Protein N-Alpha-Acetyltransferase., Thomas Arnesen, Matthew J Betts, Frédéric Pendino, David A Liberles, Dave Anderson, Jaime Caro, Xianguo Kong, Jan E Varhaug, Johan R Lillehaug
Characterization Of Hard2, A Processed Hard1 Gene Duplicate, Encoding A Human Protein N-Alpha-Acetyltransferase., Thomas Arnesen, Matthew J Betts, Frédéric Pendino, David A Liberles, Dave Anderson, Jaime Caro, Xianguo Kong, Jan E Varhaug, Johan R Lillehaug
Department of Medicine Faculty Papers
BACKGROUND: Protein acetylation is increasingly recognized as an important mechanism regulating a variety of cellular functions. Several human protein acetyltransferases have been characterized, most of them catalyzing epsilon-acetylation of histones and transcription factors. We recently described the human protein acetyltransferase hARD1 (human Arrest Defective 1). hARD1 interacts with NATH (N-Acetyl Transferase Human) forming a complex expressing protein N-terminal alpha-acetylation activity. RESULTS: We here describe a human protein, hARD2, with 81 % sequence identity to hARD1. The gene encoding hARD2 most likely originates from a eutherian mammal specific retrotransposition event. hARD2 mRNA and protein are expressed in several human cell lines. …
Utility Of Health Belief Model Constructs In Predicting Dietary Behaviors Among Female University Students: A Pilot Investigation, Vanessa Emily Anderson
Utility Of Health Belief Model Constructs In Predicting Dietary Behaviors Among Female University Students: A Pilot Investigation, Vanessa Emily Anderson
Electronic Theses and Dissertations
This study examined the relationship between Health Belief Model constructs as they related to dietary behaviors in a sample of university women 18-to-25 years of age (n=182). A cross-sectional, non-experimental design was utilized. Independent sample t-tests compared the mean of body mass indices (BMI) to dietary risk and race. Odds ratios amassed relationships between race and healthy food choices. Nearly one-third (32.4%) of participants were either overweight or obese. Black females had significantly higher BMI than white females; however, dietary risk from less-than-adequate fruit and vegetable consumption and consumption of high-fat foods were not related to race. Focus group participants …
Tooth-Size Discrepancy And Bolton's Ratios: A Literature Review, Othman S. A., Harradine N. W.
Tooth-Size Discrepancy And Bolton's Ratios: A Literature Review, Othman S. A., Harradine N. W.
Siti Adibah Othman
OBJECTIVES: To review the literature on Bolton's tooth-size discrepancies (TSD) with specific attention to the prevalence of TSD, and the possible influence of different classes of malocclusion, gender and racial group. Also examined were the validity of the standard deviations from Bolton's samples as an indicator of significant TSD, methods of measurement of TSD and their reproducibility. Based on the review, suggestions are made as to how future work could be improved. RESULTS AND CONCLUSIONS: Studies have reported from 20 to 30% of people with significant tooth-size anterior discrepancies and 5-14% for overall TSD. Bolton's original sample was appropriate for …