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Microbial Growth And Biofilm Formation In Geologic Media Is Detected With Complex Conductivity Measurements, Caroline A. Davis, Estella A. Atekwana, Eliot A. Atekwana, Lee D. Slater, Silvia Rossbach, Melanie R. Mormile

Geosciences and Geological and Petroleum Engineering Faculty Research & Creative Works

Complex conductivity measurements (0.1-1000 Hz) were obtained from biostimulated sand-packed columns to investigate the effect of microbial growth and biofilm formation on the electrical properties of porous media. Microbial growth was verified by direct microbial counts, pH measurements, and environmental scanning electron microscope imaging. Peaks in imaginary (interfacial) conductivity in the biostimulated columns were coincident with peaks in the microbial cell concentrations extracted from sands. However, the real conductivity component showed no discernible relationship to microbial cell concentration. We suggest that the observed dynamic changes in the imaginary conductivity (σ″) arise from the growth and attachment of microbial cells and …