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Role Of Red Gsu In Stress Response And Fe(Iii) Reduction In Geobacter Sulfurreducens, Laurie N. Didonato, Sara A. Sullivan, Barbara A. Methѐ, Kelly P. Nevin, Reg England, Derek Lovley
Role Of Red Gsu In Stress Response And Fe(Iii) Reduction In Geobacter Sulfurreducens, Laurie N. Didonato, Sara A. Sullivan, Barbara A. Methѐ, Kelly P. Nevin, Reg England, Derek Lovley
Kelly Nevin
Geobacter species are key members of the microbial community in many subsurface environments in which dissimilatory metal reduction is an important process. The genome of Geobacter sulfurreducens contains a gene designated relGsu, which encodes a RelA homolog predicted to catalyze both the synthesis and the degradation of guanosine 3′,5′-bispyrophosphate (ppGpp), a regulatory molecule that signals slow growth in response to nutrient limitation in bacteria. To evaluate the physiological role of RelGsu in G. sulfurreducens, a relGsu mutant was constructed and characterized, and ppGpp levels were monitored under various conditions in both the wild-type and relGsu mutant strains. In the wild-type …
Biofilm And Nanowire Production Leads To Increased Current In Geobacter Sulfurreducens Fuel Cells, Gemma Reguera, Kelly P. Nevin, Julie S. Nicoll, Sean F. Covalla, Trevor L. Woodard, Derek Lovley
Biofilm And Nanowire Production Leads To Increased Current In Geobacter Sulfurreducens Fuel Cells, Gemma Reguera, Kelly P. Nevin, Julie S. Nicoll, Sean F. Covalla, Trevor L. Woodard, Derek Lovley
Kelly Nevin
Geobacter sulfurreducens developed highly structured, multilayer biofilms on the anode surface of a microbial fuel cell converting acetate to electricity. Cells at a distance from the anode remained viable, and there was no decrease in the efficiency of current production as the thickness of the biofilm increased. Genetic studies demonstrated that efficient electron transfer through the biofilm required the presence of electrically conductive pili. These pili may represent an electronic network permeating the biofilm that can promote long-range electrical transfer in an energy-efficient manner, increasing electricity production more than 10-fold.