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2006

Iowa State University

Agricultural Science

Series

Dengue virus

Articles 1 - 1 of 1

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Oscillating Kissing Stem-Loop Interactions Mediate 5′ Scanning-Dependent Translation By A Viral 3′-Cap-Independent Translation Element, Aurélie Mamisoa Rakotondrafara, Charlotta Polacek, Eva Harris, W. Allen Miller Jan 2006

Oscillating Kissing Stem-Loop Interactions Mediate 5′ Scanning-Dependent Translation By A Viral 3′-Cap-Independent Translation Element, Aurélie Mamisoa Rakotondrafara, Charlotta Polacek, Eva Harris, W. Allen Miller

Plant Pathology and Microbiology Publications

The 3′-untranslated regions (UTRs) of a group of novel uncapped viral RNAs allow efficient translation initiation at the 5′-proximal AUG. A well-characterized model is the Barley yellow dwarf virus class of cap-independent translation elements (BTE). It facilitates translation by forming kissing stem-loops between the BTE in the 3′-UTR and a BTE-complementary loop in the 5′-UTR. Here we investigate the mechanisms of the long-distance interaction and ribosome entry on the RNA. Upstream AUGs or 5′-extensions of the 5′-UTR inhibit translation, indicating that, unlike internal ribosome entry sites in many viral RNAs, the BTE relies on ...