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2006

Iowa State University

Agricultural Science

Series

Agronomy Publications

Articles 1 - 4 of 4

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Determining The Extent Of Frost Damage In Maize Seed Using The Tetrazolium Test, Mindy Devries, A. Susana Goggi Dec 2006

Determining The Extent Of Frost Damage In Maize Seed Using The Tetrazolium Test, Mindy Devries, A. Susana Goggi

Agronomy Publications

Frost damage in seed corn causes a significant decrease in seed quality. The tetrazolium (TZ) test has been used to identify types of physiological damage in several plant species, including frost damage in corn. Quantifying the amount of seed damaged by frost and the consequent loss of seed vigor are important for making management decisions. The current tetrazolium test procedures provide seed viability information but do not estimate seed vigor. In our project, the tetrazolium staining procedure was modified to allow separation of viable seeds into vigor categories. Vigor categories were defined based on Association of Official Seed Analysts (AOSA ...


Light Is Essential For Degradation Of Ribulose-1,5-Bisphosphate Carboxylase-Oxygenase Large Subunit During Sudden Death Syndrome Development In Soybean, J. Ji, M. P. Scott, M. K. Bhattacharyya Jan 2006

Light Is Essential For Degradation Of Ribulose-1,5-Bisphosphate Carboxylase-Oxygenase Large Subunit During Sudden Death Syndrome Development In Soybean, J. Ji, M. P. Scott, M. K. Bhattacharyya

Agronomy Publications

Fusarium solani f. sp. glycines (Fsg) has been reported to produce at least two phytotoxins. Cell-free Fsg culture filtrates containing phytotoxins have been shown to develop foliar sudden death syndrome (SDS) in soybean. We have investigated the changes in protein profiles of diseased leaves caused by cell-free Fsg culture filtrates prepared from Fsg isolates. Two-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis (PAGE) was conducted to investigate the protein profiles of diseased and healthy leaves. An approximately 55 kDa protein was found to be absent in diseased leaves. Matrix-assisted laser desorption-ionization time-of-flight mass spectrometric analyses and a database search revealed that the ...


Dehydrin-Like Proteins In Soybean Seeds In Response To Drought Stress During Seed Filling, N. H. Samarah, R. E. Mullen, S. R. Cianzio, P. Scott Jan 2006

Dehydrin-Like Proteins In Soybean Seeds In Response To Drought Stress During Seed Filling, N. H. Samarah, R. E. Mullen, S. R. Cianzio, P. Scott

Agronomy Publications

There is no information on accumulation of dehydrin proteins during seed development and maturation of soybean [Glycine max (L.) Merr.] in response to drought stress. Our objective was to study accumulation of dehydrin-like proteins in developing soybean seeds in response to drought stress. A greenhouse experiment and a field experiment were conducted. In the greenhouse experiment, three treatments were imposed on soybean plants after beginning of linear seed filling (R5): well-watered (WW), gradual stress (GS) imposed before severe stress, and sudden severe stress (SS). In the field treatments were irrigation (I) and nonirrigation (NI) (rainfed) conditions imposed from R ...


Breeding For Grain Amino Acid Composition In Maize, Audrey Darrigues, Kendall R. Lamkey, M. Paul Scott Jan 2006

Breeding For Grain Amino Acid Composition In Maize, Audrey Darrigues, Kendall R. Lamkey, M. Paul Scott

Agronomy Publications

Improving the amino acid balance of grain has been a long-standing objective of plant-breeding research. In this chapter, we review the history of maize breeding for improved amino acid balance. Following this, we present results of our experiments involving divergent selection for the levels of the amino acids tryptophan and methionine in random-mated populations.