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Assessment Of Interactions Amoung Viruses Infecting Sweetpotato, Charalambos D. Kokkinos Jan 2006

Assessment Of Interactions Amoung Viruses Infecting Sweetpotato, Charalambos D. Kokkinos

LSU Doctoral Dissertations

Viral diseases, especially those caused by mixed infections, are among the economically most important diseases of sweetpotato. Real-time PCR assays were developed for the detection and quantification of the potyviruses Sweet potato feathery mottle virus (SPFMV), Sweet potato virus G (SPVG), Ipomoea vein mosaic virus (IVMV); the crinivirus Sweet potato chlorotic stunt virus (SPCSV), and the begomovirus Sweet potato leaf curl virus (SPLCV) directly from infected sweetpotato plants. Titers of SPFMV, IVMV, and SPVG were lower in singly-infected sweetpotato plants compared to singly-infected plants of the standard indicator host Brazilian morning-glory (Ipomoea setosa) and the standard propagation host I. nil ...


Agrobacterium Tumefaciens-Mediated Transformation Of Tobacco (Nicotiana Tabacum L.) Leaf Disks: Evaluation Of The Co-Cultivation Conditions To Increase Beta-Glucuronidase Gene Activity, Sunjung Park Jan 2006

Agrobacterium Tumefaciens-Mediated Transformation Of Tobacco (Nicotiana Tabacum L.) Leaf Disks: Evaluation Of The Co-Cultivation Conditions To Increase Beta-Glucuronidase Gene Activity, Sunjung Park

LSU Master's Theses

Agrobacterium tumefaciens-mediated transformation is generally used for genetic transformation of higher plants. Several experimental factors important for the increase of beta-glucuronidase (GUS) reporter gene activity were evaluated in this study using leaf disks of tobacco (Nicotiana tabacum L. cv. Xanthi). We found that co-cultivation temperature at 20C is the most critical factor to obtain the reproducible enhancement of GUS activity. pCAMBIA 1305.01 resulted in higher GUS activity than the other two pCAMBIA vectors 1301 and 1305.02. The highest GUS activity and transformation efficiency were achieved under the following experimental conditions: Agrobacterium tumefaciens strain LBA4404 containing pCAMBIA1305.01 was ...