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Accelerating Micro-Scale Pcr (Polymerase Chain Reactor) For Modular Lab-On-A-Chip System, Pin-Chuan Chen

LSU Master's Theses

The polymerase chain reaction (PCR) is a powerful technique used to exponentially amplify specific DNA sequences of interest through repetitive temperature cycling. The temperatures used in PCR are typically 90°C - 94°C for denaturation, 50°C - 70°C for renaturation, and 70°C - 75°C for extension. The double-stranded helix of nucleotides, carrying the genetic information, is separated during denaturation, reacts with chemical primers during renaturation, and becomes a complete, replica, double-stranded DNA helix structure during extension. The primary drawback of current commercial benchtop PCR machine is its cycle time due to its large thermal capacitance, and micro PCR is under developing …