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Evaluation Of Microbial Diversity In Wetland Through Polymerase Chain Reaction (Pcr) And Restriction Fragment Length Polymorphism (Rflp), Gregory K. Joseph Jun 2006

Evaluation Of Microbial Diversity In Wetland Through Polymerase Chain Reaction (Pcr) And Restriction Fragment Length Polymorphism (Rflp), Gregory K. Joseph

Theses and Dissertations

The diversity of microbial communities in wetlands has not been fully measured. These communities may offer tools to naturally remediate sites polluted with chlorinated compounds. Polmerase Chain Reaction (PCR) and Restriction Fragment Length Polymorphism (RFLP) are genomic techniques that are capable of rapidly characterizing bacteria communities and can provide valuable information about the diversity of microbial communities.


Molecular Characterization Of Wetland Soil Bacterial Community In Constructed Mesocosms, Ethan C. Bishop Jun 2006

Molecular Characterization Of Wetland Soil Bacterial Community In Constructed Mesocosms, Ethan C. Bishop

Theses and Dissertations

The use of wetlands to effectively remediate aquifers contaminated with chlorinated solvents is an emerging technique, which shows high promise. In order to better understand this process and test its legitimacy, a treatment wetland was constructed at Wright-Patterson AFB, Dayton, Ohio and, in a joint effort with Wright State University (WSU), has previously shown the effective removal of PCE. The purpose of this research was to characterize the soil bacterial community, pre-PCE injection, among three wetland plant species from the sedge family (Cyperaceae) within constructed wetland mesocosms and to identify any bacterial dominance. Carex comosa, Scirpus atrovirens, and Eleocharis erythropoda …


Investigation Of Copper Contamination And Corrosion Scale Mineralogy In Aging Drink Water Distributions Systems, Nadja F. Turek Mar 2006

Investigation Of Copper Contamination And Corrosion Scale Mineralogy In Aging Drink Water Distributions Systems, Nadja F. Turek

Theses and Dissertations

Research has shown higher levels of copper appear in drinking water conveyed through relatively new copper piping systems; older piping systems typically deliver lower copper levels in their drinking water. This research contributes field data from a real drinking water distribution system, providing a better understanding of this phenomenon, as it relates to treatment considerations and compliance with the Lead and Copper Rule. Copper pipes and copper levels were sampled from drinking water taps of 16 buildings with pipes ranging in age from less than 1 to 48 years. Water samples from each building were collected before and following a …


Modeling Study To Quantify The Benefits Of Groundwater Contaminant Source Remediation, David E. Wagner Mar 2006

Modeling Study To Quantify The Benefits Of Groundwater Contaminant Source Remediation, David E. Wagner

Theses and Dissertations

The purpose of this research was to quantify the benefits of groundwater contaminant source remediation by using a model to simulate how reduction of source mass was related to reduction of contaminant concentration at a receptor. This thesis sought to answer three research questions: (1) how are contaminant source mass reduction and reduction of mass flux leaving the source-zone related; (2) how can we quantify the effect of the important natural attenuation processes that act upon the dissolved contaminant as it travels from the source zone to downgradient receptors, and (3) under specified site conditions, what is the maximum contaminant …


Comparative Analysis Of Biosurveillance Methodologies, David M. Kempisty Mar 2006

Comparative Analysis Of Biosurveillance Methodologies, David M. Kempisty

Theses and Dissertations

The purpose of this research is to compare two different biosurveillance methodologies: BioWatch and "A Hot Idea". BioWatch is fielded and operating in major US cities today. Air samples are collected on filter paper and analyzed for the presence of harmful biological agents. "A Hot Idea" is an evolving methodology using the human body's immune response to identify the onset of infection from a harmful pathogen. Detecting a temperature increase, using infrared thermographers, in a statistically significant portion of population would allow earlier identification of a biological release, accelerating initiation of response actions. A selected population including policemen, firemen, and …


Evaluation Of Chlorinated Solvent Removal Efficiency Among Three Wetland Plant Species: A Mesocom Study, Jun Yan Mar 2006

Evaluation Of Chlorinated Solvent Removal Efficiency Among Three Wetland Plant Species: A Mesocom Study, Jun Yan

Theses and Dissertations

Different species of plants need to be studied individually to compare the remediation efficiency of each species. This research will study three different wetland plants species and an unplanted control, under a laboratory setting. Each plant has a different characteristic favorable for chlorinated solvent degradation. Eleocharis erythropoda (Spike Rush) are plants with thin tube like leaves and large root mass. Carex comosa (Bearded Sedge) has broad leaves and Scirpus atrovirens (Green Bulrush) are broad leafed wetland plants with a long flowering stem during reproduction. PCE will be injected into the plant mesocosm and any possible PCE degradation will be observed. …


Validation Of Methods To Measure Mass Flux Of A Groundwater Contaminant, Hyouk Yoon Mar 2006

Validation Of Methods To Measure Mass Flux Of A Groundwater Contaminant, Hyouk Yoon

Theses and Dissertations

In this study, flux measurements obtained using two methods are compared with known mass fluxes in a meso-scale three-dimensional artificial aquifer. One method, the tandem recirculating well (TRW) method, is applied using two different techniques. One technique is simple and inexpensive, only requiring measurement of heads, while the second technique requires conducting a tracer test. The second method, the integrated pump test (IPT) method, requires use of one or more pumping and observation wells in various configurations. The results of the experiments in the artificial aquifer show that the most expensive technique, the TRW method using tracers, provides the most …


Selecting Energy Efficient Building Envelope Retrofits To Existing Department Of Defense Building Using Value Focused Thinking, David M. Pratt Mar 2006

Selecting Energy Efficient Building Envelope Retrofits To Existing Department Of Defense Building Using Value Focused Thinking, David M. Pratt

Theses and Dissertations

The Department of Defense (DoD) has hundreds of thousands of facilities in its inventory, which consume billions of BTUSs of energy per year. Much of that energy is used to heat and cool the facility, and a great deal of this energy is lost through the building envelope. While new military construction works towards energy efficiency, the majority of DoD facilities were built over forty years ago with little regard to energy efficiency, and it is these facilities that have the greatest potential for energy efficient building envelope retrofits. There are hundreds of various new building envelope technologies available to …


Analysis Of Bacterial Population And Distribution In The Developing Strata Of A Constructed Wetland Used For Chlorinated Ethene Bioremediation, Milton J. Clausen Jr. Mar 2006

Analysis Of Bacterial Population And Distribution In The Developing Strata Of A Constructed Wetland Used For Chlorinated Ethene Bioremediation, Milton J. Clausen Jr.

Theses and Dissertations

Chlorinated hydrocarbons and their degradation products are among of the most common organic groundwater contaminates in the United States. These compounds attack the central nervous system in animals and can affect the photosynthesis of plants. These compounds are also resistant to degradation in the environment and, because of this, pose a risk to any ecosystem in which they are present. This study identified the dominant microbial species in a constructed treatment wetland at Wright-Patterson AFB, Dayton, Ohio using 16S rRNA gene sequence analysis. Samples were taken from three different depths and during each of the four seasons. These samples were …


Isolation Of Legionella Pneumophila From Well-Maintained Emergency Showers And Eyewash Stations, Jessica Mae Myers Jan 2006

Isolation Of Legionella Pneumophila From Well-Maintained Emergency Showers And Eyewash Stations, Jessica Mae Myers

Theses and Dissertations

Legionella pneumophila is a gram-negative bacterium responsible for Legionnaire's disease, and is commonly transmitted via aerosolized water. Legionella colonization of emergency eyewash and shower stations may pose an exposure hazard to users of these stations. There is little information about the role of these stations as significant reservoirs for Legionella. Samples were collected from 67 stations in an industrial facility. At the time of this study, the stations within this facility were under a routine maintenance program that included at least monthly flushing. This study also included the analysis for other bacterial organisms to determine an association between the presence …


A Gis And Remote Sensing Based Analysis Of Impervious Surface Influences On Bald Eagle (Haliaeetus Leucocephalus) Nest Presence In The Virginia Portion Of The Chesapeake Bay, Jennifer M. Ciminelli Jan 2006

A Gis And Remote Sensing Based Analysis Of Impervious Surface Influences On Bald Eagle (Haliaeetus Leucocephalus) Nest Presence In The Virginia Portion Of The Chesapeake Bay, Jennifer M. Ciminelli

Theses and Dissertations

GIS (Geographic Information Systems) and remote sensing techniques were used to predict relationships between bald eagle nest presences and land type, distance to land type and impervious surface cover area. Data plots revealed bald eagle nest presence decreases in response to an increase in area of bareland; increases with an increase in area of forested land; decreases with an increase in distance (m) to shoreline, and decreases in response to an increase in area of impervious surfaces. Logistic regression models identified impervious surfaces as an indicator for bald eagle nest presence (P 24% as unsuitable. Unsuitable area covered 17.82% of …