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Genetic Analysis Of White Marlin (Tetrapturus Albidus) Stock Structure, John Graves, Jan Mcdowell Nov 2006

Genetic Analysis Of White Marlin (Tetrapturus Albidus) Stock Structure, John Graves, Jan Mcdowell

VIMS Articles

The genetic basis of stock structure of white marlin (Tetrapturus albidus Poey, 1860) was inferred from analyses of five tetranucleotide repeat microsatellite loci (n = 214) and the mitochondrial (mt)DNA control region (n = 99) of white marlin from four geographic regions in the Atlantic Ocean. Considerable genetic variation was present in all collections for all molecular markers. Analysis of replicate collections taken in different years from three regions revealed no significant differences in the distribution of allele frequencies among years within regions. The value of global F-statistics for both multilocus microsatellite data and mtDNA control region sequences approached significance …


Phylogeny Of Recent Billfishes (Xiphioidei), Bb Collette, Jan Mcdowell, John Graves Oct 2006

Phylogeny Of Recent Billfishes (Xiphioidei), Bb Collette, Jan Mcdowell, John Graves

VIMS Articles

Billfishes are genetically and morphologically distinct enough from scombroids to merit placement in a separate suborder, Xiphioidei. Two extant families are usually recognized: Xiphiidae (swordfish, Xiphias) and Istiophoridae, currently containing three genera, Istiophorus (sailfishes), Makaira (marlins), and Tetrapturus (spearfishes, white, and striped marlins). Phylogenetic analyses of molecular data from mitochondrial and nuclear gene sequences (mitochondrial control region, ND2, 12S, and nuclear MN 32 regions) show a different picture of relationships. Makaira is not monophyletic: blue marlin cluster with sailfish and placement of black marlin is unstable. Accepting the molecular phylogeny gives two possible classifications: (1) two genera: blue marlin + …


Detection Of Panulirus Argus Virus 1 (Pav1) In The Caribbean Spiny Lobster Using Fluorescence In Situ Hybridization (Fish), Cw Li, Jeffrey D. Shields, Hamish J. Small, Kimberly S. Reece, Et Al Sep 2006

Detection Of Panulirus Argus Virus 1 (Pav1) In The Caribbean Spiny Lobster Using Fluorescence In Situ Hybridization (Fish), Cw Li, Jeffrey D. Shields, Hamish J. Small, Kimberly S. Reece, Et Al

VIMS Articles

Panulirus argus Virus 1 (PaV1) is the first virus known to be pathogenic to a wild lobster. It infects the Caribbean spiny lobster P. argus from the Florida Keys, and has a predilection for juveniles. The monitoring of the virus in wild populations and study of its behavior in the laboratory require the development of reliable diagnostic tools. A sensitive and specific fluorescence in situ hybridization (FISH) assay was developed for detection of PaV1. The lower detection limit using a 110 bp DNA probe in a dot-blot hybridization for PaV1 DNA was 10 pg of cloned template PaV1 DNA and …


Genetic Heterogeneity Of Atlantic Bluefin Tuna Caught In The Eastern North Atlantic Ocean South Of Iceland, J Carlsson, Jr Mcdowell, Et Al Jul 2006

Genetic Heterogeneity Of Atlantic Bluefin Tuna Caught In The Eastern North Atlantic Ocean South Of Iceland, J Carlsson, Jr Mcdowell, Et Al

VIMS Articles

Atlantic bluefin tuna (Thunnus thynnus) are currently managed by the member nations of the International Commission for the Conservation of Atlantic Tunas (ICCAT) as distinct western and eastern stocks, separated by the 45 degrees W meridian. Previous studies of Atlantic bluefin tuna caught in the northeast Atlantic south of Nor-way suggested mixing of putative stocks in the region, based on abrupt shifts in the size and condition of fish during the fishing season. By contrast, more recent studies south of Iceland reported only small differences in size of tuna caught at different times of the season in that area. To …


Incentive-Based Approaches To Sustainable Fisheries, Rq Grafton, R Arnason, Et Al, Je Kirkley, Et Al Mar 2006

Incentive-Based Approaches To Sustainable Fisheries, Rq Grafton, R Arnason, Et Al, Je Kirkley, Et Al

VIMS Articles

The failures of traditional target-species management have led many to propose an ecosystem approach to fisheries to promote sustainability. The ecosystem approach is necessary, especially to account for fishery-ecosystem interactions, but by itself is not sufficient to address two important factors contributing to unsustainable fisheries: inappropriate incentives bearing on fishers and the ineffective governance that frequently exists in commercial, developed fisheries managed primarily by total-harvest limits and input controls. We contend that much greater emphasis must be placed on fisher motivation when managing fisheries. Using evidence from more than a dozen natural experiments in commercial fisheries, we argue that incentive-based …


Fish Assemblages Found In Tidal-Creek And Seagrass Habitats In The Suwannee River Estuary, Troy D. Tuckey, Mark Dehaven Jan 2006

Fish Assemblages Found In Tidal-Creek And Seagrass Habitats In The Suwannee River Estuary, Troy D. Tuckey, Mark Dehaven

VIMS Articles

Fish assemblages were investigated in tidal-creek and seagrass habitats in the Suwannee River estuary, Florida. A total of 91,571 fish representing 43 families were collected in monthly seine samples from January 1997 to December 1999. Tidal creeks supported greater densities of fish (3.89 fish/m(2); 83% of total) than did seagrass habitats (0.93 fish/m(2)). We identified three distinct fish assemblages in each habitat: winter-spring, summer, and fall. Pinfish (Lagodon rhomboides), pigfish (Orthopristis chrysoptera), and syngnathids characterized seagrass assemblages, whereas spot (Leiostomus xanthurus), bay anchovy (Anchoa mitchilli), silversides (Menidia spp.), mojarras (Eucinostomus spp.), and fundulids characterized tidal-creek habitats. Important recreational and commercial …


Liver Carcinogenesis In A Non-Migratory Fish: The Association With Polycyclic Aromatic Hydrocarbon Exposure, Wolfgang K. Vogelbein, M. A. Unger Jan 2006

Liver Carcinogenesis In A Non-Migratory Fish: The Association With Polycyclic Aromatic Hydrocarbon Exposure, Wolfgang K. Vogelbein, M. A. Unger

VIMS Articles

Field and laboratory studies indicate a strong positive association between exposure to chemical pollutants in aquatic environments and development of neoplasia in fishes. This brief communication reviews some of the more important North American and European studies that have been conducted on this relationship. We then review work conducted on a small nonmigratory estuarine cyprinodontid teleost fish, the mummichog (Fundulus heteroclitus) in the industrialized Elizabeth River, Virginia USA. Histopathological surveys of mummichogs from variously degraded habitats indicate an association between PAH exposure and development of neoplasia. We have observed non-neoplastic lesions, preneoplasms and hepatic, biliary, exocrine pancreatic and vascular neoplasms …


Molecular Detection Of Hematodinium Spp. In Norway Lobster Nephrops Norvegicus And Other Crustaceans, Hamish J. Small, Dm Neil, Ac Taylor, Et Al Jan 2006

Molecular Detection Of Hematodinium Spp. In Norway Lobster Nephrops Norvegicus And Other Crustaceans, Hamish J. Small, Dm Neil, Ac Taylor, Et Al

VIMS Articles

The Norway lobster Nephrops norvegicus (L.) from the coastal waters of Scotland is seasonally infected by a parasitic dinoflagellate of the genus Hematodinium. Methods used to detect infection include a morphological index (pleopod diagnosis) and several immunoassays. The present study describes the development and application of a set of Hematodinium-specific polymerase chain reaction (PCR) primers and DNA probes based on Hematodinium ribosomal DNA (rDNA). In the PCR assay, a diagnostic band of 380 bp was consistently amplified from total genomic DNA isolated from Hematodinium-infected N. norvegicus. The sensitivity of the assay was 1 ng DNA, which is equivalent to 0.6 …


Standard And Routine Metabolic Rates Of Juvenile Sandbar Sharks (Carcharhinus Plumbeus), Including The Effects Of Body Mass And Acute Temperature Change, W Wesley Dowd, Richard Brill, Peter Bushnell, John A. Musick Jan 2006

Standard And Routine Metabolic Rates Of Juvenile Sandbar Sharks (Carcharhinus Plumbeus), Including The Effects Of Body Mass And Acute Temperature Change, W Wesley Dowd, Richard Brill, Peter Bushnell, John A. Musick

VIMS Articles

Standard and routine metabolic rates (SMRs and RMRs, respectively) of juvenile sandbar sharks (Carcharhinus plumbeus) were measured over a range of body sizes (n=34) and temperatures normally associated with western Atlantic coastal nursery areas. The mean SMR Q(10) (increase in metabolic rate with temperature) was 2.9 +/- 0.2. Heart rate decreased with increasing body mass but increased with temperature at a Q(10) of 1.8-2.2. Self-paired measures of SMR and RMR were obtained for 15 individuals. Routine metabolic rate averaged 1.8 +/- 0.1 times the SMR and was not correlated with body mass. Assuming the maximum metabolic rate of sandbar sharks …


Survival Of White Marlin (Tetrapturus Albidus) Released From Commercial Pelagic Longline Gear In The Western North Atlantic, David Kerstetter, John E. Graves Jan 2006

Survival Of White Marlin (Tetrapturus Albidus) Released From Commercial Pelagic Longline Gear In The Western North Atlantic, David Kerstetter, John E. Graves

VIMS Articles

To estimate postrelease survival of white marlin (Tetrapturus albidus) caught incidentally in regular commercial pelagic longline fishing operations targeting swordfish and tunas, short-duration popup satellite archival tags (PSATs) were deployed on captured animals for periods of 5-43 days. Twenty (71.4%) of 28 tags transmitted data at the preprogrammed time, including one tag that separated from the fish shortly after release and was omitted from subsequent analyses. Transmitted data from 17 of 19 tags were consistent with survival of those animals for the duration of the tag deployment. Postrelease survival estimates ranged from 63.0% (assuming all nontransmitting tags were evidence of …


Growth And Maturity Of Salmon Sharks (Lamna Ditropis) In The Eastern And Western North Pacific, And Comments On Back-Calculation Methods, Kenneth J. Goldman, John A. Musick Jan 2006

Growth And Maturity Of Salmon Sharks (Lamna Ditropis) In The Eastern And Western North Pacific, And Comments On Back-Calculation Methods, Kenneth J. Goldman, John A. Musick

VIMS Articles

Age and growth estimates for salmon sharks (Lamna ditropis) in the eastern North Pacific were derived from 182 vertebral centra collected from sharks ranging in length from 62.2 to 213.4 cm precaudal length (PCL) and compared to previously published age and growth data for salmon sharks in the western North Pacific. Eastern North Pacific female and male salmon sharks were aged up to 20 and 17 years, respectively. Relative marginal increment (RMI) analysis showed that postnatal rings form annually between January and March. Von Bertalanffy growth parameters derived from vertebral length-at-age data are L-proportional to=207.4 cm PCL, k=0.17/yr, and t(0)=-2.3 …


Estimating Consumption Rates Of Juvenile Sandbar Sharks (Carcharhinus Plumbeus) In Chesapeake Bay, Virginia, Using A Bioenergetics Model, W Wesley Dowd, Richard Brill, Peter Bushnell, John A. Musick Jan 2006

Estimating Consumption Rates Of Juvenile Sandbar Sharks (Carcharhinus Plumbeus) In Chesapeake Bay, Virginia, Using A Bioenergetics Model, W Wesley Dowd, Richard Brill, Peter Bushnell, John A. Musick

VIMS Articles

Using a bioenergetics model, we estimated daily ration and seasonal prey consumption rates for six age classes of juvenile sandbar sharks (Carcharhinus plumbeus) in the lower Chesapeake Bay summer nursery area. The model, incorporating habitat and species-specific data on growth rates, metabolic rate, diet composition, water temperature (range 16.8-27.9 degrees C), and population structure, predicted mean daily rations between 2.17 +/- 0.03 (age-0) and 1.30 +/- 0.02 (age-5) % body mass/day. These daily rations are higher than earlier predictions for sandbar sharks but are comparable to those for ecologically similar shark species. The total nursery population of sandbar sharks was …