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Flexibility Revealed By The 1.85 Å Crystal Structure Of The Β Sliding-Clamp Subunit Of Escherichia Coli Dna Polymerase Iii, Aaron J. Oakley, Pavel Prosselkov, Gene Wijffels, Jennifer L. Beck, Matthew Cj Wilce, Nicholas E. Dixon
Flexibility Revealed By The 1.85 Å Crystal Structure Of The Β Sliding-Clamp Subunit Of Escherichia Coli Dna Polymerase Iii, Aaron J. Oakley, Pavel Prosselkov, Gene Wijffels, Jennifer L. Beck, Matthew Cj Wilce, Nicholas E. Dixon
Faculty of Science, Medicine and Health - Papers: part A
The subunit of the Escherichia coli replicative DNA polymerase III holoenzyme is the sliding clamp that interacts with the (polymerase) subunit to maintain the high processivity of the enzyme. The protein is a ring-shaped dimer of 40.6 kDa subunits whose structure has previously been determined at a resolution of 2.5 Å [Kong et al. (1992), Cell, 69, 425-437]. Here, the construction of a new plasmid that directs overproduction of to very high levels and a simple procedure for large-scale purification of the protein are described. Crystals grown under slightly modified conditions diffracted to beyond 1.9 Å at 100 …
Social Support As A Mediator Of Attachment Style And Depression In Adolescents., Amy Silverman
Social Support As A Mediator Of Attachment Style And Depression In Adolescents., Amy Silverman
Electronic Theses and Dissertations
No abstract provided.
Development Of A New Durable Aluminum-Silicon Alloy For The Next Generation Of Engine Block Casting., Robert Ian Mackay
Development Of A New Durable Aluminum-Silicon Alloy For The Next Generation Of Engine Block Casting., Robert Ian Mackay
Electronic Theses and Dissertations
No abstract provided.