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1998

Agriculture

Clemson University

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A New Synthetic Amphidiploid In Cucumis From A C. Sativus X C. Hystrix F1 Interspecific Hybrid, Jeffrey Adelberg, Jinfeng Chen, Jack E. Staub, Halina Skorupska, Billy Rhodes Jan 1998

A New Synthetic Amphidiploid In Cucumis From A C. Sativus X C. Hystrix F1 Interspecific Hybrid, Jeffrey Adelberg, Jinfeng Chen, Jack E. Staub, Halina Skorupska, Billy Rhodes

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CT. A synthetic amphidiploid was developed through organogenesis in tissue culturefrom an interspecific F1hybrid betweenCucumis sativus L. andC. hystrix Chakr. The resultsfrom flow cytometry indicated that, on average, 7.3% of the regenerants with unique morphology were chromosome-doubled F1hybrids. The 2C DNA content of the original F1hybrid was1.17 pg, and the 4C DNA content was 2.35 pg. Frequency of chromosome doubling between thetwo genotypes used in this study varied considerably.