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A New Species Of Heterorhabditis From The Hawaiian Islands, Scott Lyell Gardner, S. Patricia Stock, Harry K. Kaya Feb 1994

A New Species Of Heterorhabditis From The Hawaiian Islands, Scott Lyell Gardner, S. Patricia Stock, Harry K. Kaya

Harold W. Manter Laboratory of Parasitology: Faculty and Staff Publications

A new species of nematode of the genus Heterorhabditis (Nemata: Heterorhabditidae) was found during a survey of the soil entomopathogenic nematode fauna of the Hawaiian Islands. Heterorhabditis hawaiiensis sp. n. can be separated from all other species of Heterorhabditis by the length of the infective juvenile and the morphological characters of the spicules, gubemaculum, and bursa. Random amplified polymorphic DNA (RAPD) fragment analysis showed that this species also has a distinct genetic pattern in RAPD bands relative to the other 6 species or isolates of Heterorhabditis that were compared.


Characterization Of Heterorhabditis Isolates By Pcr Amplification Of Segments Of Mtdna And Rdna Genes, Susan A. Joyce, Ann M. Burnell, Thomas O. Powers Jan 1994

Characterization Of Heterorhabditis Isolates By Pcr Amplification Of Segments Of Mtdna And Rdna Genes, Susan A. Joyce, Ann M. Burnell, Thomas O. Powers

Department of Plant Pathology: Faculty Publications

Restriction digests of amplified DNA from the mitochondrial genome and the nuclear ribosomal internally transcribed spacer region have been evaluated as genetic markers for species groups in Heterorhabditis. Six RFLP profiles have been identified. These profiles supported groupings determined by cross-breeding studies and were in agreement with less definitive groupings based on other biochemical and molecular methods. Digestion patterns of both amplification products provided strong evidence for the recognition of species groups, which include Irish, NW European, tropical, and a H. bacteriophora complex. The H. bacteriophora complex could be further resolved into three genotypes represented by H. zealandica, …