Digital Commons Network^™

Mathematical Simulation Of Proteins Separation In A Packed Bed, Jing James Huang

Dissertations

The generalized adsorption models are developed to simulate the unsteady state mass transfer behavior in a packed column. Based on the nature of adsorbent particles, the adsorption models may be classified into two categories: (1) the surface adsorption model, (2) the pore diffusion model. In the first model, it is assumed that the internal diffusion is negligible, and the adsorption rate is determined by external diffusion and surface adsorption. In the second model, the effect of internal diffusion is considered as significant as that of external diffusion. For both models, the effect of axial dispersion in fluid phase is emphasized. …

Characterization Of Trihalomethane Formation From Proteins In Chlorinated Water, Robert Aaron Kravitz

Chemistry & Biochemistry Theses & Dissertations

Traditionally humates have been considered the most significant precursor in the formation of trihalomethanes in finished drinking water. This study investigates another potential precursor, namely proteins. The chlorination of aqueous protein solutions yields significant levels of trihalomethanes in terms of the maximum contaminant level (MCL) of 0.100 mg/L imposed by the USEPA. Molar yields for several proteins were determined. The average yield per unit of organic carbon was 0.5% as compared to 0.8% for a humic acid studied. A model protein, bovine serum albumin, was studied extensively to ascertain the effects of total organic carbon, chlorine dose, and time on …

Two-Dimensional Electrophoretic Studies Of Normal Spematozoal Proteins, Ying Lin

Chemistry & Biochemistry Theses & Dissertations

Protein profiles of human spermatozoa have been mapped by using high resolution two-dimensional electrophoresis which combines isoelectric focusing as the first dimension and polyacrylamide gradient gels electrophoresis in SDS as the second dimension. The profiles of normal spermatozoa! extraction, acrosomal extraction and seminal plasma showed high resolution in its separation capability. No qualitative or quantitative differences were observed by using Coomassie Brilliant Blue R-250 staining in a much higher concentration of proteins. However, coomassie blue lacks the sensitivity to detect many minor components. Therefore, a silver staining procedure was used, which resulted in greatly increased sensitivity.

Samples from normal fertile …