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A Comparison Of The Relative Magnitude Of Combinations Of Relapse Types In Rats, Mary Bridges

Theses

Applied behavior analysis uses scientifically derived methods to create treatments for socially significant behaviors. A threat to these successful treatments is the recurrence of previously reduced behavior (i.e., relapse). Relapse can be categorized into several types depending on the variables that induce relapse. Three types prevalent in clinical settings are reinstatement (i.e., induced by re-exposure to reinforcers or stimuli paired with the target behavior), renewal (i.e., induced by changes in context), and resurgence (i.e., induced by worsening of alternative reinforcement). Because relapse is harmful to long-term treatment maintenance, prior research has developed and tested mitigation strategies for these relapse types, …

Evaluating The Effects Of Ketamine On Cognitive Flexibility In Rats Using A Probabilistic Reversal Learning Task, Anthony N. Nist

All Graduate Theses and Dissertations, Fall 2023 to Present

Depression is one of the most debilitating and widespread mental health conditions in the world today. Drugs that are traditionally prescribed to combat depression are flawed in several ways, and because of this, new treatments are needed. One drug that seems capable of overcoming the limitations of traditional antidepressants is ketamine. In clinical research, a single dose of ketamine can significantly reduce symptoms of depression quickly, its effects may last for weeks to months, and its side effects appear to be limited and relatively harmless. However, clinical research is ongoing, and more research is needed to fully understand ketamine's beneficial …

Control Of Pausing In Rich-Lean Transitions By Spatially Localized And Spatially Diffuse Stimuli, Carson S. Yahrmarkt

All NMU Master's Theses

Signaled transitions from rich (favorable) to lean (less favorable) schedules of reinforcement (i.e., rich-lean transitions) can disrupt operant behavior. Previous literature suggests that disruptions in these transitions could occur because individuals engage in other behavior that reduces contact with the schedule-correlated stimuli. My thesis project evaluated this possibility by using different stimuli to manipulate the ease by which rats could reduce contact with schedule-correlated stimuli during signaled rich-lean transitions. We arranged a two-component multiple fixed ratio-fixed ratio (FR-FR) schedule to evaluate effects of different types of schedule-correlated stimuli on pausing in rich-lean transitions using rat subjects. Components had equivalent FR …

Sexual Dimorphism Of Glomerular Capillary Morphology In Rats, Zackarias Coker

Undergraduate Honors Theses

Chronic kidney disease (CKD) progresses faster in males than females; however, the underlying mechanisms remain poorly understood. Sex differences in glomerular capillary morphology has been hypothesized to contribute, in part, to the increased susceptibility to hypertension-induced renal injury and CKD progression in males, but this has not been investigated. The goal of the present study was to assess glomerular capillary morphology in male vs. female rats with intact kidneys and after uninephrectomy (UNX). We hypothesized that glomerular capillary radii (R_CAP) and length (L_CAP) would be greater in male rats.

Male (n=4) and female (n=4) with intact …

Trial Spacing And The Conditioned Motivational Effects Of A Food-Predictive Cue, Gabrielle M. Sutton

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Stimuli in the environment can come to influence motivation and behavior through a process known as Pavlovian conditioning. During Pavlovian conditioning, stimuli in the environment come to predict the availability of a reward. Two different procedures are used to investigate how stimuli can modify ongoing behavior and reward consumption, known as Pavlovian-instrumental transfer and potentiated feeding, respectively. In other procedures that investigate how stimuli modify behavior, certain time intervals during Pavlovian training can influence how much a stimulus can modify behavior. One of those intervals is the time between the presentation of a stimulus and the associated reward. This interval …

Effects Of Oxycodone And Methylphenidate On Self-Control With Aversive Outcomes, Jeremy Saul Langford

Graduate Theses, Dissertations, and Problem Reports

In the context of choice, one is said to show self-control under numerous conditions in which consideration is given to the delayed outcomes of each option. This can be difficult: both reinforcing and aversive outcomes become less effective as they are increasingly delayed. Several socially significant issues arise from a failure of delayed, aversive outcomes to impact choice, especially when immediate, reinforcing outcomes are available. Identifying the conditions under which choice is sensitive to delayed outcomes is critical to shifting choices toward alternatives in which contact with delayed, aversive outcomes is minimized. Two experiments were conducted with the aim of …

Female Middle Aged Rats Showed Increased Recovery From A Focal Demyelination With Drug Combination : Changes In Dcx And Nfkb Suggest Increased Oligodendrocytes Production, Andrew James Smith

Browse all Theses and Dissertations

Neurodegenerative diseases such as multiple sclerosis affect millions of people around the globe, and promising treatments to counter its progress have been studied extensively. In our study, we used Dawley rats and tested them using the Montoya Staircase test to evaluate their ability to use their forelimbs. After training, surgery was performed using lysolecithin to induce focal demyelination on the rats. After the surgery a drug combination of fluoxetine, ibuprofen, and ascorbic acid was used on injured rats and they were then retested on the Montoya Staircase test. They were then sacrificed and were stained for doublecortin (DCX) and nuclear …

Reducing Placental Oxidative Stress In A Rat Model Preeclampsia Using Vegfr2 Nanoparticles, Sophia Ganios

Williams Honors College, Honors Research Projects

Preeclampsia is a complication of pregnancy, where the placenta lacks sufficient blood flow due to abnormal formation of the vascular endothelial cells. This results in hypertension and increased reactive oxygen species, causing oxidative stress. The VEGF (vascular endothelial growth factor) helps develop new growth, so by increasing the receptors VEGFR2 by injection of LTP nanoparticles into the uterine wall of RUPP rats, one can see if there would be improvement in the otherwise reduced uterine pressure pregnant rat. This would be confirmed by the 8-isoprostane test, which measures oxidative stress.

Mechanistic Insight Into Traumatic Brain Injury Induced Neuronal Membrane Disruption: Cathepsin B Relocalization And A Neun Negative Cortical Neuron Subpopulation, Martina L. Hernandez

Theses and Dissertations

Traumatic brain injury (TBI) is a mechanical insult to the head that leads to brain damage and, in turn, causes long-term sensory, motor, cognitive, and affective dysfunction. Diffuse pathologies seen following such injury are associated with these life-altering outcomes that impact the daily lives of TBI survivors. The diffuse pathology that this body of work focuses on is neuronal membrane disruption; it is characterized by increased permeability of the neuron’s plasma membrane. Moreover, our lab had previously found that membrane disruption is exacerbated with intracranial pressure (ICP) elevation. We set out to measure the duration of membrane disruption following injury …

The Effects Of Raspberry Consumption On Angiotensin Ii-Induced Oxidative Stress And Fibrosis In The Kidney Of Sprague-Dawley Rats, A Secondary Analysis, Lena Lear

Nutrition Theses

Kidney disease currently affects one in seven American adults, with over 130,000 newly diagnosed adults in 2019 alone. Although there are several risk factors attributed to the development of chronic kidney disease, high blood pressure is considered the second most common risk factor behind diabetes, causing 29% of cases. The elevated levels of angiotensin II associated with high blood pressure can lead to increased levels of oxidative stress, causing damage to the kidneys.The progression of kidney disease can be slowed by following doctors’ orders of prescribed medications to manage the comorbidities of CKD and consuming foods that assist in prolonging …

Modulating Ketamine’S Locomotor Activating And Reinforcing Effects Through Drug Combinations, Tegshjargal Baasansukh

Theses and Dissertations

Major Depressive Disorder (MDD) affects approximately 280 million people worldwide. The standard of care for pharmacological treatment of MDD has been monoaminergic antidepressants (MAA). However, MAAs have serious clinical limitations including a relatively slow onset of action and up to 40% of patients failing to respond and being diagnosed as “treatment resistant”. Ketamine, an N-methyl-D-aspartate antagonist, was originally approved for use as an injectable anesthetic. In addition, ketamine has been shown to rapidly improve depressive symptoms in patients with treatment resistant depression. In 2019, (S)-ketamine was approved as a nasal spray (Spravato®) for patients with MDD and treatment-resistant depression in …

Black Gold, Natalie Peart

Capstones

Jae Lee is an urban farmer and keeper of New York City soil. Lee spends her time working across gardens throughout New York City. At Phoenix Community Garden in Ocean Hill, Brooklyn, she devises a community composting plan and picks up food scraps from local cafes. She then takes those food scraps to Phoenix Community Garden where she and some of the garden members process those food scraps and turn it into "black gold" or compost. Lee believes that a large part of her role on earth is nourishing the soil. However, New York City gardens are not only inhabited …

Building A Biomechanical Model Of A Rat Forelimb, Joshua Nathaniel Eric Mak

Dissertations and Theses

This paper presents a biomechanical model of the rat forelimb to test theories of determining viscoelastic muscle parameters. Several biomechanical models of rat hindlimbs have been developed and have explored the effects of multi-muscle control during locomotion. The forelimb model uses two ball-and-socket joints to model clavicle and scapula movement. A third ball-and-socket joint is used at the shoulder and two hinge joints are used at the elbow and wrist. Scapula motion is further constrained by muscle and spring elements. Each forelimb has 11 degrees of freedom, and 23 Hill-type muscles. The model has been created in Animatlab, which includes …

Pain And Inflammation Due To Whole-Body Vibration In A Rat Model, Folly Martha Dzan Patterson

Theses and Dissertations

Low back pain is a leading cause of disability and is associated with whole-body vibration exposure in industrial workers and military personnel. The pathophysiological mechanisms by which whole-body vibration causes low back pain have been studied in vivo, but there is little data that improve diagnosis of low back pain. The overall objective of this research was to elucidate diagnostic biomarkers associated with whole-body vibration. Hence, a rat model for vibration-induced inflammatory responses was developed. Von Frey filaments were used to determine the withdrawal threshold of the hind paw as a surrogate behavioral marker for pain. The concentration of nerve …

Long-Term Impacts Of Acute Stressor Exposure On Locus Coeruleus Function And Anxiety-Like Behavior In Rats, Olga Borodovitsyna

Graduate School of Biomedical Sciences Theses and Dissertations

Stress is a physiological state characterized by behavioral arousal that occurs during exposure to harmful or threatening stimuli, and usually facilitates an adaptive behavioral response. The persistence of stress sometimes causes it to become maladaptive, potentially contributing to disease development, including physiological complications with altered neuroendocrine signaling and impaired function of organ systems, and psychological conditions including depression and anxiety. Anxiety disorders in particular are associated with a history of stress and are the most common class of mental disorders, with a lifetime prevalence of 33.7% in the general population. The locus coeruleus (LC) is a major node in the …

An Experimental Analysis Of The Classic Overjustification Effect In Rats: Does The Removal Of “Expected” Rewards Alter Behavior In Rats As It Does In Humans?, Eleah Sunde

Master's Theses and Doctoral Dissertations

The overjustification effect posits that rewards undermine intrinsic motivation, such that an individual’s engagement in previously intrinsically motivating tasks will decrease when a previously reliable reward is withdrawn. This study aimed to determine if this overjustification effect can be demonstrated in rats, with typical confounds related to social and verbal aspects of reward delivery controlled for. Baseline “intrinsic” wheel-running rates of seven SpragueDawley rats were compared to rates that followed administration and withdrawal of a contingent sucrose reward, and to rates of rats in control conditions (n = 7). Consistent with the author’s hypothesis, the reward group’s wheel-running did not …

Pharmacological Manipulation Of Nmda Receptor Activation And Synaptic Norepinephrine Levels: Effects On Sustained Attention In Male Rats, Zach V. Redding

Electronic Theses and Dissertations

Impaired attention is common in many neurological disorders. Normal attention promotes the selective processing of important sensory information. This selective processing relies on neurotransmitters, like glutamate, and neuromodulators, like norepinephrine, acting in frontal, parietal, and visual cortices. We tested treatments targeting the glutamatergic and noradrenergic systems using a rat model of attentional lapses.Rats were trained to respond quickly to stimuli in a two-choice reaction time task (2CRTT). Response times were split into initiation time (IT) and movement time (MT). Performance measures were derived from IT and MT distributions. IT mode represents sensorimotor processing speed when rats are attentive. IT deviation …

A Translational Examination Of Alternative-Response Discrimination Training And Resurgence, Kaitlyn O. Browning

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Individuals with neurodevelopmental disorders such as autism spectrum disorders often engage in severe forms of problem behavior. Reward-based behavioral interventions are highly effective at reducing levels of problem behavior and teaching more appropriate and adaptive alternative behaviors. Despite successful reduction in problem behavior during treatment, problem behaviors are susceptible to reoccurrence or relapse. Resurgence is a type of behavioral relapse that is particularly relevant to the treatment of problem behavior and may occur following the worsening of conditions of a more recently learned alternative behavior. That is, if the rewards that were used to teach the alternative behavior are removed …

Effects Of Repeated Intermittent Episodes Of Social Stress On The Acquisition And Extinction Of A Reward-Seeking Task, Nikki Sullivan

Honors Theses

Repeated exposure to stress is known to have a myriad of effects on the brain, contributing to the development of psychiatric disorders, such as anxiety, depression, and drug addiction. For example, rats undergoing repeated social stress develop increased cocaine self-administration. These effects of stress are not well-understood and are related to changes in the brain reward system. This study investigated the effects of repeated social stress on reward-seeking behavior via the acquisition and extinction of a discriminative stimulus (DS) task and on anxiety-like behavior in the elevated plus maze (EPM). Male rats underwent intermittent social defeat (4 sessions in 10 …

A Comparison Of Response-Contingent And Response-Independent Autoshaping Trials In Rats, Jenna E. Gaskins

Theses and Dissertations

Autoshaping is a procedure, combining both operant conditioning and classical conditioning, used in animal training to jump-start a target behavior. Lepper and Petursdottir (2017) found that response-contingent pairings (RCP) were more effective than responseindependent pairings (RIP) in producing vocalizations in children with Autism Spectrum Disorder (ASD). RIP procedures entail a time-based intertrial interval (ITI) followed by the beginning of a trial, whereas RCP procedures include a response initiation period between the ITI and the trial. The current study compared RCP and RIP procedures to determine which one was more effective for acquisition of nose poking in rats. Number of days …

A Temporal Information-Theoretic Model Of Suboptimal Choice, Paul J. Cunningham

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Humans and animals often make decisions not in their long-term best interest. In one example, called suboptimal choice, pigeons sacrifice food for food-predictive stimuli. The study of suboptimal choice can reveal insights into the role of reward-predictive stimuli in maladaptive decision-making that characterizes numerous behavioral disorders. However, there is currently little evidence that rats engage in suboptimal choice, thereby raising questions about the species-generality of suboptimal choice. According to the temporal information-theoretic model, developed in Chapter 2, suboptimal choice emerges when pigeons pay more attention to the bits of temporal information conveyed by food-predictive stimuli than the rate of food …

Influences Of Salvinorin A And Sex Differences On Depressive- And Anxiety-Like Behaviors In A Chronic Mild Stress Paradigm, Sarah Mitchell

Theses

Kappa opioid receptors are colocalized with dopamine receptors and are frequently associated with depression, anxiety, stress, and drug use and abuse. KOR antagonism has widely been considered anti-depressive and anxiolytic in animal models while agonism is pro-depressive and anxiogenic. However, recent findings suggest that SalvA, a natural kappa agonist derived from a plant in the mint family, can reduce depressive- and anxiety-like behavior in rats. The current study investigated the effects of chronic mild stress on behavior, attenuation by SalvA, and sex differences. 52 Long-Evans rats, 26 males and 26 females, were exposed to six weeks of CMS. Animals received …

Establishing A Myocardial Ischemia-Reperfusion Injury Model In Mice And Rats Using Left Anterior Descending Artery Ligation And Isolated Heart Experiments, Alexander Papa

PCOM Biomedical Studies Student Scholarship

Myocardial infarction (MI) is a leading cause of death globally, with over 730,000 cases each year in the United States alone. Factors involved in the prognosis of an MI include identification of the artery occluded, the time to reperfusion, the size of the infarct, and the degree of cardiomyocyte death. Thus, the treatment of MI typically involves targeting one or more of these factors. Timely opening of an occluded artery to reperfuse the ischemic tissue remains the mainstay treatment through either thrombolytic therapy, arterial stenting, or percutaneous coronary intervention. However, reperfusion itself may cause further damage through the generation of …

Age, Access, And Sweets-Motivation, Gehan Senthinathan

Theses and Dissertations (Comprehensive)

Availability can have profound influence on the consumption of foods and drinks. The 2-phase intermittent-continuous protocol (ICP) examines sucrose solution intake in two groups of rats and finds intermittent access significantly increases intake. In Phase I, rats receive intermittent or continuous access to a 4% sucrose solution, and with adults this results in a long-term elevation (a doubling) in the intermittent group. In Phase II, when rats are shifted to common sucrose schedule, this difference is maintained. Adult rats given 16% sucrose in Phase I do not differ in consumption, but in Phase II with 4% sucrose, an unexpressed elevation …

The Role Of Autophagy In Myocardial Ischemia/Reperfusion Injury In Isolated Rat Hearts, Aloysius C. Ibe Ii

PCOM Biomedical Studies Student Scholarship

Autophagy is a housekeeping process used to remove damaged cytoplasmic constituents and protein aggregates. However, a debate persists on whether autophagy is beneficial or detrimental when an ischemic/reperfusion (I/R) insult occurs in the heart. This study tested the effects of autophagy enhancers (rapamycin and trehalose) and an autophagy inhibitor (3-methyladenine) on cardiac function and infarct size after global ischemia (30 minutes) and reperfusion (45 minutes) when given prior to ischemia (pre-treatment) or at the beginning of reperfusion (post-treatment). Rapamycin (25nM) pre-treatment and post-treatment significantly restored final left ventricular developed pressure (LVDP) to 75.4±9.1% and 60±5% of initial baseline respectively (both …

Resurgence Of Cocaine-Seeking In Rats Following Long Access And Punishment, Rusty W. Nall

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Strategies that provide access to alternative non-drug rewards are among the most effective at reducing substance use in individuals with substance use disorders, but relapse often occurs when alternative rewards are removed. Relapse induced by the loss of alternative rewards is called resurgence, and represents a challenge to otherwise effective strategies for reducing drug use. An animal model has been useful for studying resurgence, but the extant model has two limitations. First, humans usually refer to the negative consequences of drug use as the reason they stop taking drugs, but the extant model uses drug unavailability to reduce drug seeking. …

An Examination Of Affective Blunting In Rodents, Sigrid Crowel

All NMU Master's Theses

Major Depressive Disorder (MDD) is the leading cause of disability worldwide. The prevalence of MDD is 12% in men and 20% in women. Antidepressant drugs are the first line of treatment in Major Depressive Disorder and other mood disorders, particularly selective-serotonin reuptake inhibitors. Affective blunting is a potential side effect of chronic SSRI treatment, which can be defined as a diminished response to pleasurable or unpleasant stimuli, and is indicated as a marked indifference towards engagement in activities. The present study looked to examine the effects of chornic fluoxetine (0.16g/L) administration on pre-test and post-test performance of a light aversion …

Effects Of Corticosterone In Drinking Water, Samantha A. Corey

Honors Capstones

Every day we experience some type of stress. High levels of stress are a common contributor to health problems. In our lab we have previously found that exposure to 10 days of unpredictable stress reduced motivation for a sucrose reward (Bond, Anderson, McWaters, & Matuszewich, 2015). When stress occurs, our bodies release the stress hormone cortisol (corticosterone in animals). The present study was designed to assess whether administering corticosterone through drinking water would elevate blood levels of corticosterone and therefore it can be used to accurately simulate chronic stress in rats. In this study rat’s plasma corticosterone levels were evaluated …

Quantification Of Myocardial Mechanics In Left Ventricles Under Inotropic Stimulation And In Healthy Right Ventricles Using 3d Dense Cmr, Zhan-Qiu Liu

Theses and Dissertations--Mechanical Engineering

Statistical data from clinical studies indicate that the death rate caused by heart disease has decreased due to an increased use of evidence-based medical therapies. This includes the use of magnetic resonance imaging (MRI), which is one of the most common non-invasive approaches in evidence-based health care research. In the current work, I present 3D Lagrangian strains and torsion in the left ventricle of healthy and isoproterenol-stimulated rats, which were investigated using Displacement ENcoding with Stimulated Echoes (DENSE) cardiac magnetic resonance (CMR) imaging. With the implementation of the 12-segment model, a detailed profile of regional cardiac mechanics was reconstructed for …

A High Fructose Diet Alters Affective-Like Behavior And Metrics Of Synaptic Mitochondrial Function Differentially In Male And Female Rats, Alix H. Kloster

Theses and Dissertations

Fructose consumption has become a normalized part of the standard American diet over the past 40 years. While fructose consumption is a known risk factor of metabolic syndrome, there is increasing evidence that fructose consumption influences brain and behavior. Recently, more interest has been focused on mitochondrial dysfunction as a potential link between metabolic stress and modifications of the central nervous system. Mitochondria are in the unique position of both regulating and being vulnerable to alterations in energy homeostasis. Sex-differences are well categorized in the presentation of metabolic symptoms associated with excessive fructose consumption. Thus, it is important to characterize …