Open Access. Powered by Scholars. Published by Universities.®
- Discipline
Articles 1 - 8 of 8
Full-Text Articles in Entire DC Network
Development Of A Stereochemistry Concept Inventory, Alexey Leontyev
Development Of A Stereochemistry Concept Inventory, Alexey Leontyev
Dissertations
No abstract provided.
New Methods For Measuring Transient Interactions Between Proteins And Curved Membranes, Philip P. Cheney
New Methods For Measuring Transient Interactions Between Proteins And Curved Membranes, Philip P. Cheney
Electronic Theses and Dissertations
Variations in the physical deformation of the plasma membrane play a significant role in the sorting and behavior of the proteins that occupy it. Determining the interplay between membrane curvature and protein behavior required the development and thorough characterization of a model plasma membrane with well defined and localized regions of curvature. This model system consists of a fluid lipid bilayer that is supported by a dye-loaded polystyrene nanoparticle patterned glass substrate. As the physical deformation of the supported lipid bilayer is essential to our understanding of the behavior of the protein occupying the bilayer, extensive characterization of the structure …
Photogenerated O-Azaxylylenes: Mechanistic Studies And Synthetic Applications, William Cole Cronk
Photogenerated O-Azaxylylenes: Mechanistic Studies And Synthetic Applications, William Cole Cronk
Electronic Theses and Dissertations
This research sets out to build upon excited state o-azaxylylene cycloaddition. The mechanism behind the excitation and cycloaddition process of photogenerated o-azaxylylenes was determined experimentally. Time-correlated single-photon counting, steady-state spectroscopy, triplet quenching experiments, and quantum yield studies provided evidence suggesting that excited state intramolecular proton transfer is followed by intersystem crossing and stepwise addition to the tethered unsaturated pendant.
In keeping with the principles of diversity oriented synthesis, a modular approach was taken to gain access to a diverse array of N,O,S-Polyheterocycles which were modified postphotochemically via Suzuki coupling to yield fused biaryls. Cycloaddition products, outfitted with halogens …
Species Preference Of Viral Deubiquitinating Proteases Toward Isg15 Through Structural And Enzymatic Characterization, Michelle Kay Deaton
Species Preference Of Viral Deubiquitinating Proteases Toward Isg15 Through Structural And Enzymatic Characterization, Michelle Kay Deaton
Electronic Theses and Dissertations
Proteases from the Ovarian Tumor domain (OTU) superfamily of deubiquitinating enzymes (DUBs) are expressed by a range of RNA viruses. Viral OTUs (vOTUs) are found in nairoviruses such as Crimean Congo Hemorrhagic Fever virus (CCHFV), Nairobi sheep disease virus (NSDV), the Erve virus (ERVEV), and the Dugbe virus (DUGV), as well as in the areterivirus Porcine Reproductive and Respiratory Syndrome virus (PRRSV), among others. vOTUs, which interfere with host innate immune response through editing of host Ub and Ub-like molecules such as interferon stimulated gene 15 (ISG15), have been identified as a potential virulence factor through their role in evading …
The Effects Of Molecular Chaperones On Tau Fibril Assembly, Ahmed Omran
The Effects Of Molecular Chaperones On Tau Fibril Assembly, Ahmed Omran
Electronic Theses and Dissertations
The accumulation of microtubule-associated protein tau into fibrillar aggregates is the hallmark of Alzheimer’s disease and other neurodegenerative disorders, collectively referred to as tauopathies. Fibrils can propagate from one cell to the next and spread throughout the brain. However, a study shows that only small aggregates can be taken up by cultured neuronal cells. The mechanisms that lead to the breakage of fibrils into smaller fragments remain unknown. In yeast, the AAA+ chaperone HSP104 processes the reactivation of protein aggregates and is responsible for fragmentation of fibrils. This study focused on investigating the effects of molecular chaperones on tau fibrils …
Measurement Of Particulate Transition Metals And Atmospheric Processing Of Iron, Benton T. Cartledge
Measurement Of Particulate Transition Metals And Atmospheric Processing Of Iron, Benton T. Cartledge
Electronic Theses and Dissertations
The work presented herein details the measurement of particle-bound metals in environmental samples with specific interests in iron (Fe) in atmospheric particulate matter. Metals were measured in ambient PM2.5 to study the effects and contributions of a light rail system on the concentrations of metals in atmospheric particles. Particulate matter samples were collected on board trains, near the tracks, and at an urban background location in Denver, CO. Metals were found to be enriched in particles collected on board the trains more so than at the other locations. Fe speciation was also measured in the soluble fraction of the …
Rapid Scan Electron Paramagnetic Resonance (Epr) And Digital Epr Development, Zhelin Yu
Rapid Scan Electron Paramagnetic Resonance (Epr) And Digital Epr Development, Zhelin Yu
Electronic Theses and Dissertations
Rapid scan electron paramagnetic resonance (EPR) was developed in the Eaton laboratory at the University of Denver. Applications of rapid scan to wider spectra, such as for immobilized nitroxides, spin-labeled proteins, irradiated tooth and fingernail samples were demonstrated in this dissertation. The scan width has been increased from 55 G to 160 G. The signal to noise (S/N) improvement for slowly tumbling spin-labeled protein samples that is provided by rapid scan EPR will be highly advantageous for biophysical studies. With substantial improvement in S/N by rapid scan, the dose estimation for irradiated tooth enamels became more reliable than …
Fluorescent Biological Aerosol Particles In Coastal Canada And The Link To Atmospheric Ice Nuclei, Jixiao Li
Fluorescent Biological Aerosol Particles In Coastal Canada And The Link To Atmospheric Ice Nuclei, Jixiao Li
Electronic Theses and Dissertations
Bioaerosols are a subgroup of atmospheric aerosols and are often linked to the spread of human, animal and plant diseases. Bioaerosols also may play an indirect effect on environmental processes, including the formation of precipitation and alteration of the global climate through their role as nuclei for cloud droplet formation. Several types of biological organisms (e.g., fungi and bacteria) have been shown to be effective ice nuclei (IN) and cloud condensation nuclei (CCN).
During 21 days in August 2013 we participated in a collaborative international campaign at a rural, coastal site near the village of Ucluelet on the west coast …