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Analysis Of Function Of The Son1-Interacting Protein, Lnk2 In Arabidopsis Thaliana, Prince Kudjoe Zogli

Graduate College Dissertations and Theses

The Arabidopsis SON1 F-box protein was implicated in regulating a pathogen defense pathway, but its exact function in wild-type plants is unknown. As an F-box protein it was predicted that SON1 would assembles into a SON1-SCF ubiquitin ligase complex that recruits specific plant defense-related proteins for proteolysis. The yeast 2-hybrid assay was used to screen for potential substrates for a putative SON1-SCF ligase, leading to the identification of Arabidopsis LNK2 as a SON1-binding factor.

Comprehensive protein-protein interaction analysis has shown that the binding of SON1 to LNK2 protein is specific, because closely related, full-length Arabidopsis F-box proteins do not interact …

Abscisic Acid And Nitrate Transporter Mtlatd/Nip Signaling In Root And Nodule Development In Medicago Truncatula, Chang Zhang

Graduate College Dissertations and Theses

Abscisic acid (ABA) is a plant hormone that regulates various developmental processes and environmental stress responses. ABA modulates growth of both primary roots and lateral roots, helping to shape root architecture. The lateral root organ defective (latd) mutants, disrupted in the MtLATD/NIP gene, encoding a nitrate transporter, have severe root growth defects that can be rescued by applying ABA. However, the way in which ABA stimulates latd root growth is unclear, and the downstream components of MtLATD/NIP and ABA signaling are completely unknown. To answer these questions, this dissertation focuses on two major potential downstream regulators: Reactive Oxygen Species (ROS) …

Top-Down And Bottom-Up Tools For Integrated Pest Management In Northeastern Hop Production, Lily Calderwood

Graduate College Dissertations and Theses

The demand for locally sourced hops from Northeastern microbreweries began the recent resurgence in local hop production. The farming community has increased acreage and improved the quality of hops grown and processed in the Northeast region over the past five years. There was a sharp increase in the number of Northeast hop producers from six in 2009 to over 175 in 2014. Hop growers in the Northeast are new to the crop and have limited experience with pest identification and management. This dissertation encompasses three research projects that were conducted over the 2012-2014 growing seasons. These projects were the first …

Comprehensive Assessment Of Organic Apple Production In Vermont: Experience From Two Orchard Systems, 2006-2013, Terence L. Bradshaw

Graduate College Dissertations and Theses

Despite substantial consumer demand and willingness to pay premium prices for organically grown fruit, apple growers in Vermont and other New England states have been slow to adopt certified organic practices. Barriers cited in the past to increased adoption of organic apple production in the region include susceptibility of traditionally grown cultivars to apple scab, lack of effective insect pest management materials, and few available effective options for fruit thinning. Recent changes in apple cultivar plantings in the region, introduction of new insect pest management materials, and advances in crop thinning justified an evaluation of organic apple production systems containing …

The Efficacy And Non-Target Impacts Of An Organic Disease Management System Containing Biostimulants Compared With Two Sulfur-Based Systems On Four Apple Cultivars In Vermont, Ann L. Hazelrigg

Graduate College Dissertations and Theses

Disease management in organic apple orchards in Vermont is focused on controlling diseases with sulfur fungicides. The objective of this two year study was to evaluate the target and non-target effects of an organic disease management system containing agricultural biostimulants compared to two sulfur-based systems on foliar and fruit diseases, pest and beneficial arthropods, tree growth, yield and fruit quality on four cultivars, `Ginger Gold', `Honeycrisp' and `Liberty' and `Zestar!'. Trees were arranged in a complete randomized design of five three-tree replications in a certified organic orchard. The two sulfur-based systems differed in the number of applications; in the third …

Biological Indicators Of Compost-Mediated Disease Suppression Against The Soilborne Plant Pathogen Rhizoctonia Solani, Lynn Fang

Graduate College Dissertations and Theses

Compost can suppress soilborne plant pathogens that cause significant damage on globally important food crops. However, reports of plant pathogen suppression are inconsistent likely because there are no established standards for feedstock material, application rate, and maturity age upon application. Excellent results can be achieved in greenhouse trials, but field applications are much less reliable. Disease suppression occurs through the activity of biocontrol organisms (direct antagonism), and general microbial competition. Biocontrol species are hypothesized to colonize the pile during the curing phase, but single species may not be as important as microbial consortia. Substrate composition during maturation may give rise …