Open Access. Powered by Scholars. Published by Universities.®
- Keyword
-
- Department of Biological Sciences (15)
- Department of Pharmacology and Toxicology (10)
- Aquaporin (2)
- Department of Biochemistry and Molecular Biology (2)
- Department of Neuroscience, Cell Biology, and Physiology (2)
-
- Sarin (2)
- Skin (2)
- Trophic Cascades (2)
- 2D-difference in gel electrophoresis (1)
- 3'IgHRR (1)
- 8-OH-DPAT (1)
- ACE (1)
- ACE2 (1)
- AT1 Receptor (1)
- ATP (1)
- Acetylcholineserase (1)
- Activated Carbon (1)
- Allelopathy (1)
- Allergen (1)
- Allergens (1)
- Anion Channel (1)
- Antennal Gland (1)
- Apoptosis (1)
- Aquaglyceroporin (1)
- Aquaporin 1 (1)
- Aryl Hydrocarbon Receptor (1)
- Astrocyte (1)
- Biomarker (1)
- Biotic Homogenization (1)
- Brain Slices (1)
Articles 1 - 30 of 30
Full-Text Articles in Entire DC Network
Effects Of Chlorpyrifos-Oxon On Prohormone Convertase Enzyme Activity, Sean William Harshman
Effects Of Chlorpyrifos-Oxon On Prohormone Convertase Enzyme Activity, Sean William Harshman
Browse all Theses and Dissertations
Organophosphate (OP) compounds have been synthesized for various applications including medicine, pest control and weapons of terror. OPs irreversibly phosphorylate the active site of acetylcholinesterase, the primary target of toxicity, rendering it inactive causing a hypercholinergic state in the post synaptic cleft. However, very little is known about the effects of OPs on secondary targets of toxicity i.e. other serine hydrolases. The prohormone convertase (PC) enzymes, specifically PC1/3 and PC2, are serine hydrolases which are responsible for the processing of pro-opiomelanocortin in the anterior and intermediate lobes of the pituitary. To test the direct interaction between OPs and the PC …
The Development Of Preservice Elementary Teachers' Pedagogical Content Knowledge For Scientific Modeling, Michelle Elizabeth Cotterman
The Development Of Preservice Elementary Teachers' Pedagogical Content Knowledge For Scientific Modeling, Michelle Elizabeth Cotterman
Browse all Theses and Dissertations
For teachers to effectively engage students in scientific modeling, they require pedagogical content knowledge (PCK) for this practice. This study examines how preservice elementary teachers develop PCK for scientific modeling when given modeling centered instruction in a science methods course. Our findings indicate preservice teachers made significant pedagogical gains in this practice, shifting from using models as static products to using them as thinking tools for students to develop science content knowledge. However, few viewed learning the practice of scientific modeling as an important learning goal. Preservice teachers also had difficulty translating the depth of their understandings into lesson designs, …
Distribution And Host Specificity Of Erysiphe Cruciferarum (Powdery Mildew) Attacking Alliaria Petiolata (Garlic Mustard), In Southwestern Ohio, Victoria Lyn Ciola
Distribution And Host Specificity Of Erysiphe Cruciferarum (Powdery Mildew) Attacking Alliaria Petiolata (Garlic Mustard), In Southwestern Ohio, Victoria Lyn Ciola
Browse all Theses and Dissertations
Garlic mustard is an invasive Eurasian biennial that has rapidly spread throughout the United States. In Southwestern Ohio, many garlic mustard populations are infected with the powdery mildew Erysiphe cruciferarum that can reduce the growth and fitness of garlic mustard. E. cruciferarum was assessed using a Decision Tree in regards to its potential use as a biological control agent. I determined the distribution of E. cruciferarum on garlic mustard and I determined the potential risk of E. cruciferarum to native Brassicaceous species and selected crops. I surveyed 19 parks in Southwestern Ohio and recorded the number of diseased garlic mustard …
Clamping Of Intracellular Ph In Neurons From Neonatal Rat Brainstem During Hypercapnia, Vivian C. Nanagas
Clamping Of Intracellular Ph In Neurons From Neonatal Rat Brainstem During Hypercapnia, Vivian C. Nanagas
Browse all Theses and Dissertations
In this work, I have made attempts to clamp intracellular pH in the presence of hypercapnic acidosis (HA) in neurons from the locus coeruleus (LC) and nucleus of the solitary tract (NTS) in neonatal rat (ages P3 to P17) brainstem slices. Two approaches were used to minimize hypercapnia-induced ΔpHi: 1) an increase in intracellular buffering power with a high HEPES concentration using whole cell patching techniques in individual neurons, and 2) a weak acid diffusion technique that relies on an efflux of weak acid to counterbalance HA influx thereby clamping pHi in multiple neurons at once. pH …
Characterization Of Microtubule Depolymerization By The Hiv Protein Rev, Shimpi Bedi
Characterization Of Microtubule Depolymerization By The Hiv Protein Rev, Shimpi Bedi
Browse all Theses and Dissertations
The HIV-1 Rev protein enables the nucleocytoplasmic export of unspliced or partially spliced mRNAs that is required for the synthesis of structural proteins. By doing so, it regulates the switch to the late phase of the viral replication cycle (Cullen, 1992). This regulatory control over viral replication makes Rev an attractive target for anti-viral intervention. The development of anti-viral remedies is hindered because the three-dimensional structure of Rev has not yet been solved by X-ray crystallography and NMR. Rev, which polymerizes into regular hollow filaments at high concentrations, forms side-to-side and end-to-side interactions making it prone to aggregation and precipitation …
The Role Of Nucleotide Signaling In The Regulation Of Icl,Swell In Human 1321n1 Astrocytoma Cells, Ian C. Wenker
The Role Of Nucleotide Signaling In The Regulation Of Icl,Swell In Human 1321n1 Astrocytoma Cells, Ian C. Wenker
Browse all Theses and Dissertations
Swollen cells expel osmolytes to decrease volume in a process referred to as Regulatory Volume Decrease, or RVD. The plasma membrane current ICl,swell is ubiquitously expressed in mammalian cells and contributes significantly to RVD. In a variety of cell types, activation of ICl,swell is enhanced by nucleotide signaling. In this study, we used human astrocytoma cells to examine whether nucleotide signaling is necessary or sufficient for ICl,swell activation. Three clones of the 1321N1 human astrocytoma cell line were used for voltage clamp recordings. One clone was transfected with P2Y1 receptors, another with P2Y2 receptors and a third was devoid of …
Immunolocalization And Changes In Expression Levels Of Glyceroporin Hc-3 In Several Tissues Of Gray Tree Frogs, Hyla Chrysoscelis Under Different Physiological Conditions, Sushmita Yaganti
Browse all Theses and Dissertations
Previous studies show that aquaporin HC-3 in gray tree frogs, Hyla chrysoscelis, a homolog of mammalian AQP3 is an aqua glyceroporin also responsible for freeze tolerance. Our study tried to localize and compare the protein expression levels of aquaporin HC-3 in seven different organs of gray tree frogs under five different physiological conditions using immuno histofluorescence and western blot. HC-3 was immunolocalized in the epidermis and dermis of the skin, basolateral portion of the collecting ducts in the kidney, red blood cells in the liver and other tissues, muscle Schwann cells and the basolateral portion of the large intestine epithelial …
Characterization Of Infectivity And Pathogenesis Of Partially Reconstructed 1918 And Highly Pathogenic Avian Influenza Viruses In The Balb/C Mouse Model, John Allen Pyles
Characterization Of Infectivity And Pathogenesis Of Partially Reconstructed 1918 And Highly Pathogenic Avian Influenza Viruses In The Balb/C Mouse Model, John Allen Pyles
Browse all Theses and Dissertations
Influenza viruses are consistently responsible for an average of 20,000 deaths and 114,000 hospitalizations per year. To a great extent, these viruses always stay one step ahead of the available vaccines and people's immunity year after year because they have the ability to either mutate part of their genetic material, or to be transmitted from one species to another. That same genetic variability explains why highly pathogenic influenza viruses emerge that cause great mortality over several countries resulting in pandemics. Highly pathogenic strains of influenza A virus have emerged occasionally in recent history, producing pandemics such as the one in …
Physiological Effects On The Expression Of Aquaporin 1-Like Hc-1 In Cope's Gray Tree Frog, Hyla Chrysoscelis, Ram Naresh Pandey
Physiological Effects On The Expression Of Aquaporin 1-Like Hc-1 In Cope's Gray Tree Frog, Hyla Chrysoscelis, Ram Naresh Pandey
Browse all Theses and Dissertations
Aquaporin 1 is a cell membrane integral protein, which functions for water transport through the hydrophobic cell membrane to the cytoplasm. HC-1 is an aquaporin 1-like protein expressed in Hyla chrysoscelis and amphibian that survives at sub-freezing temperatures in its natural habitat. The goal of the current study was to investigate the expression of HC-1 during changing physiological conditions. The expression level of HC-1 was determined in skin, muscle, liver, kidney, and intestines of warm, cold, frozen, and dehydrated frogs using Western blot and immunohistofluorescence. Although HC-1 was widely distributed, the expression level varied among tissues and physiological conditions. In …
Probing A Role For Trap150 In Gene Regulation, Divya Potabathula
Probing A Role For Trap150 In Gene Regulation, Divya Potabathula
Browse all Theses and Dissertations
The mammalian cell nucleus is a highly organized cellular compartment. The nucleus is surrounded by the nuclear envelope and contains distinct domains such as nucleoli, nuclear speckles and Cajal bodies that may reflect nuclear functions such as transcription and RNA processing. Nuclear speckles are regions in the nucleus that are enriched in splicing factors and other RNA processing factors, as well as some transcription factors and the large subunit of RNA polymerase II. Mass spectrometry analysis of purified nuclear speckles revealed 146 nuclear speckle proteins of which 33 are novel nuclear speckle components. One such novel nuclear speckle protein is …
Inflammatory Cytokines In Jet Propulsion Fuel-8 Induced Irritant Contact Dermatitis In Male Fischer Rats, Thomas Joseph Kannanayakal
Inflammatory Cytokines In Jet Propulsion Fuel-8 Induced Irritant Contact Dermatitis In Male Fischer Rats, Thomas Joseph Kannanayakal
Browse all Theses and Dissertations
Contact dermatitis is one of the most common occupational diseases. Absenteeism of the affected workers and associated health care costs makes this disease an economically important issue. Irritant contact dermatitis (ICD) is a common contact dermatitis observed among workers. As each category of irritants triggers the inflammatory process by different method, studying the mechanism of ICD is difficult. Information on the mechanism of action of an irritant is indispensable for the development of effective prophylactic and therapeutic measures. In this study, we have investigated the early changes in rat skin induced by jet fuel-8 (JP-8). Although previous studies have described …
Host Defense Mechanisms Against Mycobacterium Tuberculosis, Laurin Nicole Holloway
Host Defense Mechanisms Against Mycobacterium Tuberculosis, Laurin Nicole Holloway
Browse all Theses and Dissertations
Mycobacterium tuberculosis is one of the world's most infectious diseases. Approximately 2 million people die each year from Mycobacterium tuberculosis infection and one-third of the world's population remains infected. For decades research has focused on uncovering the tactics used by Mycobacterium tuberculosis to evade host immune responses and defense mechanisms used to fight tuberculosis infection. The following review focuses on the host defense mechanisms used to combat Mycobacterium tuberculosis with an emphasis placed on the roles that Toll-like receptors (TLRs), T cells (gamma delta, CD4, CD8), and macrophages play in mounting the innate and adaptive immune responses necessary to eradicate …
Identification Of Potential Protein Biomarkers Of Low Level Kidney Degradation, Christopher Lee Woolard
Identification Of Potential Protein Biomarkers Of Low Level Kidney Degradation, Christopher Lee Woolard
Browse all Theses and Dissertations
The purpose of this research was the identification of potential dose-dependent and time-dependent serum protein biomarkers of low level kidney degradation in a rat model. Potential biomarkers were evaluated based on differential protein expression between control and dosed samples in rat serum. Proteins of interest demonstrated upregulation at a minimum 1.5 fold increase in protein concentration control versus dosed sample. In order to identify common biomarkers of kidney decrement, three nephrotoxins were chosen to target specific locations of the kidney: 1) D-Serine, which causes necrosis of the proximal straight tubules, 2) Puromycin, an antibiotic that degrades the Glomerular Basement Membrane …
Tcdd-Induced Modulation Of The Hs1,2 Enhancer Within The 3'Immunoglobulin Heavy Chain Regulatory Region, Tharu M. Fernando
Tcdd-Induced Modulation Of The Hs1,2 Enhancer Within The 3'Immunoglobulin Heavy Chain Regulatory Region, Tharu M. Fernando
Browse all Theses and Dissertations
2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) is a potent environmental toxin that inhibits immunoglobulin (Ig) gene expression in mice. Transcriptional regulation of the Ig heavy chain (IgH) involves the 3'IgH regulatory region (3'IgH RR). The murine 3'IgH RR consists of four enhancers (hs3A, hs1,2, hs3B, and hs4), which are homologous to the human 3'IgH RR enhancers (hs3, hs1,2, and hs4). In humans, a polymorphism of the hs1,2 enhancer, resulting in a varying number of tandem repeats of a 53 bp sequence, has been correlated with autoimmune diseases like IgA nephropathy and Celiac disease. The repeated sequence contains a kB and DRE binding site. Previous …
Cloning And Expression Of Aquaporin In The Antennal Gland Of Crayfish, Procambarus Clarkii, Yang Gao
Cloning And Expression Of Aquaporin In The Antennal Gland Of Crayfish, Procambarus Clarkii, Yang Gao
Browse all Theses and Dissertations
Aquaporins (AQPs) are responsible for transferring water and other small molecules across the cell membrane. Relatively little research has been conducted on AQPs in crustaceans. To study the AQPs in crayfish, I hypothesis that 1) AQP is expressed in the antennal gland (kidney analogue) of crayfish; 2) crayfish AQP will show greater expression in tissues (gill and antennal gland) associated with volume regulation; 3) AQP mRNA expression in antennal gland and gill will increase in premolt and decrease in postmolt compared with intermolt level. RT-PCR and Real-time PCR assay were applied to test the hypothesis. A partial AQP gene was …
Effects Of Direct Mechanical Ventricular Actuation On The Apoptotic Signaling Of A Failing Heart, Scott Kerns
Effects Of Direct Mechanical Ventricular Actuation On The Apoptotic Signaling Of A Failing Heart, Scott Kerns
Browse all Theses and Dissertations
Cardiovascular disease accounts for more than 40% of all deaths in the United States (AHA-2004 report). A non blood contacting ventricular assist device (VAD) can be used to treat heart failure without the complications that arise from blood contacting VADs. This study used cellular markers of heart failure as indicators of heart function in an attempt to assess if direct mechanical ventricular actuation (DMVA) support lessened the impact of heart failure in rabbits. Cell signaling proteins were monitored using enzyme activity measurements and quantitative immunoblotting with antibodies against intrinsic and extrinsic apoptotic pathways during heart failure with and without DMVA …
Novel Neuroprotectants For Sarin Plus Cbdp Induced Convulsions, Kaushal V. Joshi
Novel Neuroprotectants For Sarin Plus Cbdp Induced Convulsions, Kaushal V. Joshi
Browse all Theses and Dissertations
Sarin, also known as Sarin (German agent B) is classified as a weapon of mass destruction. Sarin (O-isopropyl methyl phosphonofluoridate) is a highly toxic nerve agent originally produced for chemical warfare and has been used in terrorist activities. Sarin is an extremely potent acetylcholinesterase inhibitor with high specificity and affinity for the enzyme. High sarin doses causes death due to anoxia resulting from airway obstruction, weakness of the muscles of respiration, respiratory failure and convulsions. Current treatments are still not effective at protecting against long term effects following exposure. A current approach aims to counteract the increased glutamatergic and cholinergic …
Due-B, A New Human Dna Replication Protein, Is The Functional Homolog Of S. Cerevisiae Sld3, Jianhong Yao
Due-B, A New Human Dna Replication Protein, Is The Functional Homolog Of S. Cerevisiae Sld3, Jianhong Yao
Browse all Theses and Dissertations
DNA unwinding elements (DUEs) are commonly found at DNA replication origins. The DUE binding protein (DUE-B) is crucial for the initiation of DNA replication in eukaryotes. The unique 59 amino acid C-terminal part of DUE-B shares nearly 50% similarity with yeast the C-terminus of Sld3. DUE-B plays a key role in eukaryotic DNA replication because it is required for the loading of Cdc45, the MCM helicase activator, on chromatin. Here we show that DUE-B, just like yeast Sld3, binds to Cdc45 and TopBP1 through its C-terminus in Sf9 cells and in vitro. We also show that DUE-B, Cdc45 and TopBP1 …
The Effects Of Coaching Strategies For Primary Prevention Of Coronary Heart Disease Involving Asymptomatic Hospital Employees, Gabriel Mario Moreno
The Effects Of Coaching Strategies For Primary Prevention Of Coronary Heart Disease Involving Asymptomatic Hospital Employees, Gabriel Mario Moreno
Browse all Theses and Dissertations
Introduction: An effective coronary heart disease (CHD) prevention program is needed. Currently, prevention of CHD is being sought through pre-CHD risk stratification of patients using office-based screening tools such as the Framingham risk model (Greenland, Smith, and Grundy, 2001). However, patients who are given recommendations for lifestyle behavior modification find difficulty employing and sustaining the changes. The recent popularity of coaching for health behavior change has prompted the question of whether coaching is an effective method to produce a higher success rate for lowering cardiovascular disease following risk stratification.
The main purpose of this study was to investigate the effectiveness …
Dose-Dependent Effects Of Oxygen On Metabolism In Rat Cortico-Hippocampal Brain Tissue Slices, Jennifer Lynne Hollyfield
Dose-Dependent Effects Of Oxygen On Metabolism In Rat Cortico-Hippocampal Brain Tissue Slices, Jennifer Lynne Hollyfield
Browse all Theses and Dissertations
Studies have shown that 95% oxygen increases neuronal excitability and ROS production. We wanted to investigate the dose-dependent effects of oxygen on brain slice metabolism. We exposed rat brain cortico-hippocampal tissue slices to 0.40, 0.95, and 4.50 ATA O2 for 60 minutes, made dual-phase tissue extracts, and used multi-nuclear NMR experiments to elucidate the slice metabolism. We found that low doses of oxygen may shift metabolism toward anaerobic glycolysis. Elevated lactate suggests this shift, along with elevated ratios of NAD+/NADH which may drive the reactions toward the production of lactate. The results also suggest that high doses of oxygen may …
In-Vitro Biocompatibility Of Silver Nanoparticles Anchored On Multi-Walled Carbon Nanotubes, Alicia Brooks Castle
In-Vitro Biocompatibility Of Silver Nanoparticles Anchored On Multi-Walled Carbon Nanotubes, Alicia Brooks Castle
Browse all Theses and Dissertations
Silver (Ag) nanoparticles were anchored on the surface of three different multi-walled carbon nanotubes (MWNTs): Ag-MWNTs (pure carbon), Ag-MWNT-COx (carbonyl and carboxyl doped), and Ag-MWNT-CNx (Nitrogen-doped). The chemical reactivity of the nanotubes was MWNTs< COx-MWNTs< CNx-MWNT. Ag-NP anchored on the MWNTs had an average size corresponding to MWNTs 6.64nm +/- 2.25nm; CNx-MWNTs, 13.24nm +/- 3.94nm, and COx-MWNT/Ag 11.75nm +/- 4.65nm. Cellular function and immune response were evaluated to determine biocompatibility of the synthesized nanomaterials (NM) on the human keratinocyte cell line (HaCaT). Cellular assays revealed toxicity after 24h however, full cellular recovery was observed at 48h. These results suggest Ag nanoparticles anchored on MWNTs have the ability to interact with cells without disrupting cellular function.
Ischemic Stroke In Type Ii Diabetic Mice: Deregulation Of Sdf-1a/Cxcr4 Axis, Avik Das
Ischemic Stroke In Type Ii Diabetic Mice: Deregulation Of Sdf-1a/Cxcr4 Axis, Avik Das
Browse all Theses and Dissertations
Type 2 diabetes mellitus is a major risk factor for ischemic stroke. Also diabetes is associated with poor outcome after stroke. Underlying mechanisms are however not fully understood. Alteration in the expression of the SDF-1a/CXCR4 axis, which is important for ischemic tissue repair, can be a probable cause. In this study, we have determined the expression of SDF-1a/CXCR4 in the brains of type II diabetic mice at basal and in response to ischemic stroke and have investigated a method for overexpression of SDF-1a in the brains of the diabetic mice. Adult male C57BLKS/J mice (db/db) of age 8 weeks were …
Risk-Sensitive Foraging Facilitates Species-Level Trophic Cascades Among Terrestrial Mammals: A Meta-Analysis, Bryan David Murray
Risk-Sensitive Foraging Facilitates Species-Level Trophic Cascades Among Terrestrial Mammals: A Meta-Analysis, Bryan David Murray
Browse all Theses and Dissertations
The widespread removal of large carnivores from terrestrial ecosystems may be contributing to plant species loss and biotic homogenization through trophic cascades. A few plants not preferred by ungulates are increasing in abundance while the majority of preferred plants are in decline. A meta-analysis of recruitment gaps in browse-sensitive tree species demonstrated that failed recruitment coincided in time with carnivore loss. In all studies recruitment continued in nearby browsing refugia. A second meta-analysis revealed that the mechanism of mammalian herbivore-mediated trophic cascades contains a behavioral component. Foraging mammals exhibited higher giving-up densities (GUDs) when under higher predation risk. GUDs were …
Effect Of Low-Dose Sarin Exposure On The Neurochemistry Of Different Brain Structures In Mice, Dhawal Pravin Oswal
Effect Of Low-Dose Sarin Exposure On The Neurochemistry Of Different Brain Structures In Mice, Dhawal Pravin Oswal
Browse all Theses and Dissertations
Sarin (GB) is a toxic organophosphate (OP) nerve agent that was released in the Gulf War and was used in terrorist attacks in Japan. People who survive such attacks exhibit various long-term effects including alterations in neuropsychological performances. It has also been hypothesized that the Gulf War Illness could be a result of low level exposure to OP's. In order to understand the effect of low dose exposure to GB on physiological and behavioral functions, we analyzed the levels of monoamines and their metabolites in different brain areas after exposure of mice to a sublethal dose of GB. Mice (male …
Impact Of Diabetes On Ace/Ace2 Balance And Angiotensin Ii Type 1 Receptor Expression In Db/Db Diabetic Mice, Malav Navinchandra Madhu
Impact Of Diabetes On Ace/Ace2 Balance And Angiotensin Ii Type 1 Receptor Expression In Db/Db Diabetic Mice, Malav Navinchandra Madhu
Browse all Theses and Dissertations
Alterations in the renin-angiotensin system (RAS) are considered to be crucial for the development of diabetic complications like hypertension and nephropathy. Our previous work demonstrated role of AT1 receptors (AT1R) in the development of hypertension in db/db diabetic mice. The aim of this study was to test the hypothesis that there is upregulation of renal AT1R and imbalance in renal ACE/ACE2 homeostasis in db/db mice. In addition, we hypothesize that treatment with an anti-hyperglycemic or an AT1R blocker will correct this imbalance. Five week old control and db/db mice were housed in metabolic cages for 24 hour collection of urine. …
A Potential Strategy To Maintain Hsv-1 In A Latent State: Use Of Immunoregulatory Peptide Mimetics, Nasrin Majidi
A Potential Strategy To Maintain Hsv-1 In A Latent State: Use Of Immunoregulatory Peptide Mimetics, Nasrin Majidi
Browse all Theses and Dissertations
This study reviews the role of interferon-gamma (IFN-gamma) in HSV-1 latency. CD8+ T cells inhibit the reactivation of HSV-1 in trigeminal ganglia (TG) by production of IFN-gamma. Although CD8+ T cells include all the cytotoxic apparatus for cytotoxicity, latently infected neuronal cells are not killed by CD8+ T cells. The CD94-NKG2a molecule on CD8+ T cells, binds to Qa-1b (a MHC class I like molecule) present on neuronal cell to inhibit CD8+ T cells cytotoxicity. Other studies have also determined that the IFN-gamma peptide mimetic 95-132 inhibits HSV-1 replication in the same way as IFN-gamma in infected cells. IFN-gamma mimetic …
Finding The Trophic Trickle: Using Herbaceous Indicator Species To Investigate Plant Recovery From Intense Browsing By White-Tailed Deer (Odocoileus Virginianus) After The Re-Colonization Of A Top Predator (Canis Lupus), Krystle A. Bouchard
Browse all Theses and Dissertations
High densities of white-tailed deer (Odocoileus virginianus) have been implicated in changing forest community structure and composition. Top predators, including gray wolf (Canis lupus), were extirpated from much of their range by the mid 1900s, but have since returned to Northern Wisconsin. To determine whether the re-colonization of wolves could initiate a trophic cascade resulting in the recovery of understory plants from deer browsing, I surveyed four herbaceous species in areas without wolves and areas with 4-6 year old wolf packs and 12-13 year old wolf packs. Plant size and reproduction were greater in areas where wolves had been for …
Influence Of Diet On Population Growth And Allergen Production In Cultured House Dust Mites - Dermatophagoides Farinae And Dermatophagoides Pteronyssinus, Swetha Avula Poola
Influence Of Diet On Population Growth And Allergen Production In Cultured House Dust Mites - Dermatophagoides Farinae And Dermatophagoides Pteronyssinus, Swetha Avula Poola
Browse all Theses and Dissertations
Dermatophagoides farinae and Dermatophagoides pteronyssinus culture extracts are used for skin testing, immunotherapy and research studies. There is a need to develop an effective culture method so that standardized extracts and allergens can be produced. The purpose of this study was to examine if different diets effect mite population growth and the amount of allergen produced in cultures. Dermatophagoides farinae and Dermatophagoides pteronyssinus were cultured on diets A and B. The density of mites, life stage composition and allergen concentration was determined at two week intervals. Enzyme-linked immune sorbent assays (ELISA) were used to assess group 1 and group 2 …
Competition And Allelopathic Effects Of Native And Invasive Populations Of Lonicera Maackii: A Comparative Analysis, Daniel M. Romanek
Competition And Allelopathic Effects Of Native And Invasive Populations Of Lonicera Maackii: A Comparative Analysis, Daniel M. Romanek
Browse all Theses and Dissertations
It is unknown if the novel weapons or evolution of increased competitive ability hypotheses explain the invasiveness of L. maackii in eastern United States woodlands. I tested if L. maackii's allelopathic properties have a significant impact on the fitness of native Pilea pumila in addition to below ground competition as well as if L. maackii populations vary in allelopathic and/ or competitive ability within the invasive range and between native and invasive ranges. Addition of activate carbon to potting soil increased the ability of L. maackii to inhibit the fitness of P. pumila in addition to competition. L. maackii …
The Influence Of Temperature On Population Growth And Allergen Production In Cultured House Dust Mites - Dermatophagoides Pteronyssinus And Dermatophagoides Farinae, Lakshmi Yella
Browse all Theses and Dissertations
Dermatophagoides pteronyssinus and Dermatophagoides farinae are commercially cultured to produce materials to make extracts for diagnostic tests and immunotherapy. There is some speculation as to how the culture conditions such as time of culture, temperature, relative humidity and food might influence the mite populations and subsequent production of allergens. The purpose of our investigation was to see how temperature influenced the population growth and allergen production of cultured house dust mites. D. pteronyssinus and D. farinae were cultured at 20°C and 25°C. Samples were collected at two week intervals for live mite count, life stage composition and to determine Group …