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Cloning And Characterization Of Two Floral-Specific Genes Of Sunflower (Helianthus Annuus L.)., Joggari T. Reddy
Cloning And Characterization Of Two Floral-Specific Genes Of Sunflower (Helianthus Annuus L.)., Joggari T. Reddy
Electronic Theses and Dissertations
Sunflower (Helianthus annuus) being an important oil seed crop was chosen for this study, because genes isolated and characterized from sunflower will shed more light on plant development, and can also be useful in biotechnological applications. The study involved the isolation and characterization of two sunflower floral-specific genes: SF15 (an anther-specific gene) and SF17 (a pollen-specific gene). SF15: Northern blot analysis revealed that the probe hybridized to 1.0 kb mRNA transcripts present in the young unopened disc florets, whose development was delineated into seven stages. SF15 transcripts begin to accumulate in stage 1 and reach higher levels in stages 2, …
The Influence Of Hydrological Variability On The Spatial Distribution And Orientation Of Freshwater Mussels (Bivalvia: Unionidae)., Joanne. Di Maio
The Influence Of Hydrological Variability On The Spatial Distribution And Orientation Of Freshwater Mussels (Bivalvia: Unionidae)., Joanne. Di Maio
Electronic Theses and Dissertations
An a priori selection of drainage basins in southwestern Ontario and southeastern Michigan was made using records of flow variability. The distribution and relative abundance of mussels (Bivalvia: Unionidae) was determined for three event (i.e., hydrologically flashy) and three stable (i.e., hydrologically stable) drainage basins. Multivariate analyses revealed that distinct mussel communities exist based on the hydrological variability of the river. Amblema plicata, Pyganodon grandis, and Fusconaia flava characterized event sites and Elliptio dilatata, Lampsilis radiata, and Lasmigona costata characterized stable sites. Apparently, some unionids can be associated with specific hydrological river types and their distribution can be predicted using …
Phosphotyrosine-Containing Proteins During Spore Germination Of Dictyostelium Discoideum., Mona Louise. Gauthier
Phosphotyrosine-Containing Proteins During Spore Germination Of Dictyostelium Discoideum., Mona Louise. Gauthier
Electronic Theses and Dissertations
This present study contributes to the growing hypothesis of signal transduction processes to be the underlining mechanisms which induce Dictyostelium discoideum spores to autoactivate and release nascent amoebae. This work focuses on the role of tyrosine phosphorylation during germination and the extracellular signals which govern the phosphorylation/dephosphorylation of specific protein(s) on their tyrosyl residues. Probing for phosphotyrosine-containing proteins during the germination program of this organism by Western blot analysis using anti-phosphotyrosine antibodies revealed at least ten proteins with relative mobilities between 207kDa and 32kDa. The pattern observed was shared by all strains examined, V12, NC4, SG2 and SG1, independent of …
Histochemical And Immunocytochemical Studies Of Nitric Oxide Synthase In The Olfactory System Of The Larval Stage Of The Sea Lamprey (Petromyzon Marinus L.)., Seyed Mansour. Hosseini
Histochemical And Immunocytochemical Studies Of Nitric Oxide Synthase In The Olfactory System Of The Larval Stage Of The Sea Lamprey (Petromyzon Marinus L.)., Seyed Mansour. Hosseini
Electronic Theses and Dissertations
In this investigation, I have attempted to localize nitric oxide synthase NOS in the olfactory system of one of the most primitive vertebrates, sea lamprey (Petromyzon marinus, L.), to determine if olfactory NOS is an evolutionarily conserved trait. Initially, I used NADPH diaphorase histochemistry as a specific histochemical marker for NOS activity. To show that diaphorase staining was due to NOS activity, exogenous substances expected to increase or diminish NOS enzymatic activity were added to the diaphorase assay. The following treatment with L-arginine, the diaphorase staining intensity at the supranuclear region of the olfactory epithelium was enhanced indicating that the …
Effects Of Nutrient Manipulation On Communities Of Forested And Agricultural Streams., Rebecca Gail. Boyko
Effects Of Nutrient Manipulation On Communities Of Forested And Agricultural Streams., Rebecca Gail. Boyko
Electronic Theses and Dissertations
Effects of nitrate and phosphate enrichment on the periphyton and macroinvertebrate communities were examined at six rivers flowing through both forested and agricultural land use areas in spring and autumn. Nutrient diffusing substrates (NDS) containing four nutrient treatments (C, N, P, N+P) were placed in the rivers for a 9 d incubation period. Upon retrieval, each NDS was analyzed for remaining nutrient content of the agar, periphyton accumulation (determined by chlorophyll a extraction), organic content (AFDM) of adhering materials, and macroinvertebrate colonization. NDS that were incubated in streams for 9 d were analyzed for residual nutrient content in spring and …
Habitat Use And Movement Patterns Of The Eastern Fox Snake (Elaphe Vulpina Gloydi) At Point Pelee National Park, Ontario., Catherine M. (Barrett). Watson
Habitat Use And Movement Patterns Of The Eastern Fox Snake (Elaphe Vulpina Gloydi) At Point Pelee National Park, Ontario., Catherine M. (Barrett). Watson
Electronic Theses and Dissertations
Radio telemetry was used to investigate and describe the habitat use and movement patterns of the eastern fox snake (Elaphe vulpina gloydi) at Point Pelee National Park, Ontario. Ten snakes were taken from a sample of habitats and monitored for a two year time period in 1992/1993 disclosing detailed information about habitat utilisation, home ranges and hibernaculum locations. Snakes that were tracked in both years showed significant differences in habitat use for the first three components. Each snake appeared to be significantly different in at least one of the four components of microhabitat and therefore differed in the pattern of …
Organic Contaminants In Lower Great Lakes' Waterfowl In Relation To Diet, With Particular Reference To Dreissena Polymorpha., Edward John. Mazak
Organic Contaminants In Lower Great Lakes' Waterfowl In Relation To Diet, With Particular Reference To Dreissena Polymorpha., Edward John. Mazak
Electronic Theses and Dissertations
Six species of waterfowl including greater scaup (Aythya marila), lesser scaup (Aythya affinis), bufflehead (Bucephala albeola), canvasback (Aythya valisineria), mallard (Anas platyrhynchos), and redhead (Aythya americana) were collected and analyzed for diet content, organic contaminant patterns (pesticides and polychlorinated biphenyls (PCBs)), and stable isotope ($\sp{14}$C and $\sp{15}$N) signatures from 3 sites in the lower Great Lakes (Fighting Island, western Lake Erie, and Big Creek). Lesser and greater scaup from Fighting Island were classified into groups according to the percentage dry mass of zebra mussel (Dreissena polymorpha) in the diet. Lesser and greater scaup classified as Dreissena-consumers had, on average, 85 …