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Woody Vegetation Of A Disjunct Oak Woodland In East-Central Nebraska., Debra Ann Beightol

Student Work

The woody vegetative composition of the Oak Glen Wildlife Management Area, a disjunct oak woodland in Seward County, Nebraska, was assessed using the Point-Quarter method. Based on data from three size categories, the presettlement composition of the woodland appears to have been a bur oak (Quercus macrocarpa) dominated community which, based on spacing and growth form, may have been savanna-like. Under present management, this community is succeeding to one consisting primarily of elm (Ulmus spp.), and hackberry (Celtis occidentalis)

An Investigation Of The Chlorophylls Of Selected Prasinophyte Algae., Leslie Carlat Kwasnieski

Student Work

Introduction: Chlorophyll a is the prevalent light-collecting pigment in eukaryotic, photosynthetic organisms. Several different chlorophylls exist in nature, but all autotrophic plants contain chlorophyll a (Bogorad, 1976). The majority of these chlorophyll a molecules function to absorb light and channel the excitation energy to photochemical sites in reaction centers. Chlorophylls b, c, d, and e, carotenoid pigments and phycobiliproteins are organized into antenna systems which absorb light maximally at different wavelengths. This design permits the visible light spectrum to be exploited (Foyer, 1984) for energy by different photosynthetic organisms. Chlorophyll b is present in higher plants, Chlorophyta, Prasinophyceae, and Euglenophyta; …

Gene Expression During The Latent And Replicative States Of The Epstein-Barr Virus, Ronald John Weigel

Yale Medicine Thesis Digital Library

Gene expression of the Epstein-Barr virus (EBV) was examined during the latent and lytic viral cycles. During latency the virus expresses two small RNAs (EBERs) and the EB nuclear antigen (EBNA). A gene encoding one peptide of the EBNA complex was mapped using S1 nuclease analysis. EBNA peptide is encoded by a 2028 base exon contained within the EBV Bam HI K fragment; the 3' end of this exon occurs 30 bp after the polyadenylation signal AATAAA and the 5' end occurs within a splice acceptor site. The latent state was disrupted experimentally by treatment with phorbol ester or superinfection …

Recent Ponderosa Pine (Pinus Ponderosa) Occurrence In Nebraska Sandhills Prairie., Ernest M. Steinauer

Student Work

Four stands of ponderosa pine (Pinus ponderosa), situated within sandhills prairie sites in north central Nebraska, were evaluated for age distribution, reproductive status and spatial distribution. Trees were generally young; of the 563 individuals sampled, 11 had establishment dates prior to 1900 with the oldest dated 1868. All trees greater than 55 years old bore cones. Initial tree establishment appears to have occurred on north facing slopes where both the oldest trees and 92% of all trees observed were located. Age structure data show pine populations are increasing in all four prairie sites. This study shows that, under present management, …

Facilitative And Inhibitory Interactions Among Estuarine Meiobenthic Harpacticoid Copepods (Commensalism, Benthos, Louisiana)., George Thomas Chandler Ii

LSU Historical Dissertations and Theses

In Louisiana, USA, marshes, the harpacticoid copepods Scottolana canadensis and Pseudostenhelia wellsi are community co-dominants with similar lifestyles. Scottolana canadensis is a semi-sessile burrow-dweller capable of subsurface suspension and deposit feeding. Pseudostenhelia wellsi is a semi-sessile tube-building copepod that continuously corkscrews back and forth within its tube and appears to graze on the inner tube walls. Monospecific patches of both species (250 (.) 5cm('-2)) were generated in laboratory microcosms to determine their effects on colonization by two completely errant, burrowing harpacticoids, Nitocra lacustris and Cletocamptus deitersi, that have similar foraging and burrowing behaviors and similar effects on sediment structure. Pseudostenhelia …

Studies On The Molecular Cloning Of A Gene For Beta-Glucosidase From A Bacillus Cereus/Cellulomonas Sp. Hybrid Bacterium Derived Through Protoplast Fusion., Eun Soo Han

LSU Historical Dissertations and Theses

A (beta)-glucosidase gene was cloned from a cellulolytic bacterium, which was obtained by hybrid-formation between Bacillus cereus and a Cellulomonas sp. through protoplast fusion, into Escherichia coli plasmid pBR322 using recombinant DNA techniques. E. coli strain transformants of JM83 harboring this cloned plasmid were able to utilize cellobiose as a carbon source. (beta)-glucosidase activity was assayed in these cells by P-nitrophenyl (beta)-D-glucopyranoside (PNPG) hydrolysis. Hybridization of the cloned DNA fragment with DNA from the hybrid organism was performed by Southern transfer (92) and DNA/DNA dot blot procedures. The hybridization data strongly suggested that the DNA cloned into pBR322 was from …

A Mouse Model Of Bovine Pneumonic Pasteurellosis: Dose Response, Temporal Evolution Of Lesions And Effects Of Arachidonic Acid Inhibitors (Indomethacin, Nordihydroguaiaretic Acid, Hydroxyurea)., Lanny Wade Pace

LSU Historical Dissertations and Theses

Outbred Swiss Webster mice were used as an experimental model for bovine pneumonic pasteurellosis. Using light microscopy and transmission electron microscopy to evaluate lesions, it was determined that intrabronchial inoculation of Pasteurella haemolytica serotype 1 induces dose dependent pulmonary lesions in the mouse. Five x 10('7) colony forming units was the optimum dose which induced lesions and allowed survival for at least 24 hours. Using the optimum dose of inoculum, lesions were evaluated at different time points to determine when neutrophil influx into the lung first occurred. Four hours post inoculation was the minimum time required for neutrophil influx to …

Benthic Microfloral Production On The West And Gulf Coasts Of The United States: Techniques For Analyzing Dynamic Data (Entropy Data Analysis)., Gary Paul Shaffer

LSU Historical Dissertations and Theses

The objective of this study was to compare benthic microfloral production on the west and gulf coasts of the U.S.A., and to determine what factors govern that production. Intensive, month long field studies were conducted at two sites in a salt marsh lagoon (Mugu Lagoon) in southern California and at two sites in a shallow water estuary (Barataria Estuary) in southwest Louisiana. For both studies, fluctuations in production during a single month approximated those measured previously over an entire year: daily production varied from 0 to 1500 mg C (.) m('-2). A comparison was made between the sources of error …

Equine Herpesviruses Type 1 And 2: Growth And Cytopathic Expression In Organ Cultures Of Equine Respiratory Epithelium And In Cell Cultures (Electron Microscopy, Morphogenesis)., Emmanuel Eddie Brako

LSU Historical Dissertations and Theses

The pathogenesis of equine herpesvirus type 1 (EHV-1) and type 2 (EHV-2) respiratory infection was studied in cell and organ cultures by infectivity assays following experimental infections (growth kinetics), and by light, scanning and transmission electron microscopy. The cell cultures included fetal donkey and pony alveolar macrophages (FDAM/FPAM), fetal equine kidney (FEK), rabbit kidney (LLC-RK(,1)), and african green monkey kidney (VERO) cells. Organ cultures were derived from respiratory tissues (nasal, tracheal and pharyngeal mucosae) of seronegative donkey (F1D1) and pony (F1M1) fetuses, and of a seropositive adult pony (A1). In the cell cultures, EHV-1 multiplied and produced cytopathic effects (CPE) …

Circadian Lipogenic Responses To Insulin And Prolactin., Anthony Henry Cincotta

LSU Historical Dissertations and Theses

Lipogenic responsiveness to insulin was investigated at different times of day in freshly isolated hepatocytes and in primary cultured hepatocytes of Syrian hamsters, Mesocricetus auratus held on 14 hour daily photoperiods (lights on: 08.00) by following (('14)C)acetate incorporation into total cell lipid. The influence of prolactin on lipogenesis was tested by adding the hormone in vivo and directly to primary cultured hepatocytes. The findings of these studies are unique in that they demonstrate important roles of temporal interaction between prolactin and insulin in regulation of lipogenesis in cultured hepatocytes. They are summarized as follows: (1) A circadian variation of lipogenesis …

The Physiological Ecology Of A Migratory Noctuid Moth, The Velvetbean Caterpillar (Anticarsia Gemmatalis Hubner) (Density, Plant Age, Juvenile Hormone)., Howard William Fescemyer

LSU Historical Dissertations and Theses

Velvetbean caterpillars, Anticarsia gemmatalis Hubner, were reared at different densities on soybean. The degree of dark pigmen- tation was density-dependent under field and laboratory conditions. The highest degree of dark pigmentation was expressed in the last instar when larvae were crowded throughout development. Soybean plant age had no effect on the degree of dark pigmentation. Larval development was delayed and one-day-old adult weight was decreased as the larval density and plant age increased. Size and biochemical composition of one-day-old A. gemmatalis adults was investigated in relation to larval pigmentation, larval den- sity and plant age. Wet, dry and protein weights …

Functional Organization Of The Gustatory System In The Brains Of Ictalurid Catfish: A Combined Electrophysiological And Neuroanatomical Study (Taste, Viscerotopic, Sensory Maps, Forebrain)., Jagmeet Singh Kanwal

LSU Historical Dissertations and Theses

The present study utilizes electrophysiological and neuroanatomical techniques to investigate the functional organization of the gustatory system in the brainstem and the forebrain of the channel catfish, Ictalurus punctatus. Neuroanatomical studies indicate an overlapping, segmental pattern of projection of glossopharyngeal-vagal branches in the vagal lobe. The vagal nerve complex is divisible into an interoceptive input (consisting of general visceral fibers) from abdominal viscera and an exteroceptive-branchial component (consisting of special and general visceral fibers) innervating the oro-pharyngeal region. The interoceptive-visceral input converges onto the exteroceptive, oro-pharyngeal input in the nucleus intermedius of the vagal lobe (nIV). In addition, extra-oral and …

Molecular Studies Of T-Lymphocytes From Bovine Leukemia Virus-Infected Cattle., Diana Lynn Williams

LSU Historical Dissertations and Theses

Highly specific monoclonal antibodies and microfluorimetry were used to determine the absolute number of B and T lymphocytes in the peripheral blood of bovine leukemia virus (BLV) infected cattle. The peripheral blood lymphocyte populations from BLV-infected cattle were elevated when compared to BLV-negative animals. The increase in the lymphocyte population in the aleukemic (AL) animals was due to an increase in T lymphocytes. In the PL animals the increase was due mainly to an increase of B lymphocytes but also to an increase of T lymphocytes. The PL animals showed a significantly higher number of surface immunoglobulin positive cells (sIg('+)) …

Studies On The Molecular Cloning Of "Aryletherase" From A Ligninolytic Erwinia Sp.: Enhancement Of Gene Expression., Jeffrey William Cary

LSU Historical Dissertations and Theses

Recombinant DNA techniques were used to enhance the expression of a gene encoding "aryletherase" activity. This gene, apparently lacking regulatory elements, was present on a 792bp BamHI DNA fragment of Erwinia sp. genomic DNA, that was cloned into Escherichia coli using the plasmid vector pBR322. To obtain a larger fragment of Erwinia genomic DNA carrying the "aryletherase" gene and its regulatory regions, an Erwinia sp. genomic DNA library was constructed in the lambda phage EMBL3. Screening the library with nick translated 792bp BamHI fragment identified several recombinant phage which demonstrated homology with the probe. Restriction digestion of DNA from one …

Genomic Organization And Sequence Analysis Of Rabbit Muscle Phosphofructokinase Gene., Chan Ping Lee

LSU Historical Dissertations and Theses

A rabbit genomic clone containing the muscle phosphofructokinase (PFK) gene of approximately 17 kilobase pairs encoding 779 amino acids was isolated and sequenced. This gene contains 22 exons, ranging from 45 to 190 base pairs, and is split by 21 introns of 73-3500 base pairs. Eighty six percent of this gene is occupied by introns. An additional incomplete intron is found in the 5' flanking region, while an Alu-like sequence is identified in the 3' flanking region. The amino acid sequence translated from the coding sequence of this gene reveals 29 residues (amino acid numbers 479-507) which complete a previously …

Tolerance, Bioenergetics And Biochemical Composition Of The Blue Crab, Callinectes Sapidus Rathbun, Exposed To The Water-Soluble Fraction Of South Louisiana Crude Oil., Shiao Yu Wang

LSU Historical Dissertations and Theses

The tolerance, bioenergetics, growth, molting frequency and biochemical composition of juvenile blue crabs, Callinectes sapidus, exposed to the water-soluble fraction of South Louisiana crude oil were determined during a 21-d exposure period. Blue crabs were highly tolerant to petroleum hydrocarbons; the LC50 was 3927 ppb on day 21. Rates of energy intake decreased with increasing hydrocarbon concentration. Although energy expenditure rates of crabs exposed to 2504 ppb were not significantly higher than that of control crabs, they were significantly higher than those of crabs exposed to 820 and 1476 ppb. The resulting decrease in scope for growth was thus due …

Anti-Mosquito Behavior Of Adult And Juvenile White-Footed Mice (Peromyscus Leucopus) (Treehole Mosquito, Aedes Triseriatus, Epidemiology), Connie Isaacs

Doctoral Dissertations

Rodents seldom are identified as hosts for mosquitoes, based on serological analysis of bloodmeals. However, due to limited survey and lack of sensitivity in most bloodmeal analyses, host species can be misrepresented or undetected. Knowledge of behavior of a potential host species can complement results of bloodmeal analysis, because active vertebrates may prevent mosquito feeding and thus rarely serve as hosts. The objectives of this study were to characterize anti-mosquito behavior in the white-footed mouse (Peromyscus leucopus noveboracensis) and to examine effectiveness of such behavior in mice based on age, prior exposure to mosquitoes, and immediate environment.

I used wild-stock …

Strain Analysis In The Bullbreen Group (Early Silurian), Central Western Spitspbergen, Using Antitaxial Extension Veins., Matthew Edward. Dodt

Electronic Theses and Dissertations

Source: Masters Abstracts International, Volume: 40-07, page: . Thesis (M.Sc.)--University of Windsor (Canada), 1986.

The Geology And Geochemistry Of The Cartwright Lake Area Lynn Lake Greenstone Belt, Northwestern Manitoba., David C. Peck

Electronic Theses and Dissertations

Dept. of Geology and Geological Engineering. Paper copy at Leddy Library: Theses & Major Papers - Basement, West Bldg. / Call Number: Thesis1986 .P435. Source: Masters Abstracts International, Volume: 40-07, page: . Thesis (M.Sc.)--University of Windsor (Canada), 1986.

The Structural Geology Of Poly-Deformed Low-Grade Metasediments In A Portion Of Tudor Township, Southeastern Ontario., Barbara Marie. Gossiaux

Electronic Theses and Dissertations

Dept. of Geology and Geological Engineering. Paper copy at Leddy Library: Theses & Major Papers - Basement, West Bldg. / Call Number: Thesis1986 .G677. Source: Masters Abstracts International, Volume: 40-07, page: . Thesis (M.Sc.)--University of Windsor (Canada), 1986.

Lithostratigraphy And Sedimentology Of The Late Jurassic-Early Cretaceous Mic Mac And Lower Missisauga Formations, Offshore Nova Scotia, Canada., Philip Thomas. Nantais

Electronic Theses and Dissertations

Source: Masters Abstracts International, Volume: 40-07, page: . Thesis (M.Sc.)--University of Windsor (Canada), 1986.

Further Characterization Of The Receptor For The Alpha Toxin Of Staphylococcus Aureus., David Andrew. Simpson

Electronic Theses and Dissertations

Dept. of Biological Sciences. Paper copy at Leddy Library: Theses & Major Papers - Basement, West Bldg. / Call Number: Thesis1986 .S567. Source: Masters Abstracts International, Volume: 40-07, page: . Thesis (M.Sc.)--University of Windsor (Canada), 1986.

An Ultrastructural Examination Of The Myxobacteria Cystobacter Fuscus, Melittangium Lichenicola And Polyangium Cellulosum., Gloria Ann. Berlie

Electronic Theses and Dissertations

Source: Masters Abstracts International, Volume: 40-07, page: . Thesis (M.Sc.)--University of Windsor (Canada), 1986.

The Lysosomal Trehalase Of Saccharomyces Cerevisiae., Steven David. Harris

Electronic Theses and Dissertations

Dept. of Biological Sciences. Paper copy at Leddy Library: Theses & Major Papers - Basement, West Bldg. / Call Number: Thesis1986 .H375. Source: Masters Abstracts International, Volume: 40-07, page: . Thesis (M.Sc.)--University of Windsor (Canada), 1986.

The Hepatotoxicity And Urinary Metabolites Of 2,4- And 2,6-Dimethylaniline In The Rat And Dog (Aromatic Amines)., Marcia L. Hardy

LSU Historical Dissertations and Theses

This study was designed to investigate the hepatotoxicity and urinary metabolites of 2,4- and 2,6-DMA in the dog and rat and the covalent binding of the two isomers in the rat as differences in metabolism may be related to the divergent hepatic responses. Male Beagles were orally dosed for 1 or 10 days with 2,6- or 2,4-DMA at 25 mg/kg. Male Fischer 344 rats were gavaged with 2,6- or 2,4-DMA at 25% of their respective LD50's (262.5 mg/kg; 117/mg/kg) for the same time period. The effects of phenobarbital (PB), 3-methylcholanthrene (3MC) and SKF-525A on the hepatotoxicity and metabolites of 2,4- …

The Effects Of Heartworm Infection And Beta Blockade On Submaximal, Graded Exercise In Dogs., Patricia Louise Hopkins Price

LSU Historical Dissertations and Theses

Four dogs with canine heartworm disease (CHD) and four control dogs were tested during a 21 minute submaximal, incremental, graded treadmill test, under normal conditions and after 2 mg/kg propranolol, i.v. Arterial and venous blood gas and pH, heart rate, systemic blood pressure, hematocrit, rectal temperature, lactate, glucose, and electrolytes were measured. CHD dogs had lower sodium and P(,a)O(,2) levels. All dogs displayed progressive increments in heart rate, hematocrit, and potassium and small gains in rectal temperature during exercise. Mean arterial blood pressure (MABP) and arterial pH increased and remained elevated throughout exercise. P(,v)O(,2) and P(,a)CO(,2) decreased, but P(,a)O(,2) did …

Characterization Of Messenger Ribonucleoprotein Particles In Dormant Sporangiospores Of The Fungus Mucor Racemosus (Messenger-Rna)., Charles Patrick Chapman

LSU Historical Dissertations and Theses

Extracts of sporangiospores of Mucor racemosus contained RNA that readily hybridized with ('3)H polyuridylic acid. Prior to germination, this RNA was in a form sedimenting at <80S. Within 10 minutes after initiating germination, most of this RNA sedimented with polyribosomes and 80S monoribosomes. Particulate material from spore extracts bound to oligo dT -cellulose at high ionic strength and was assumed to contain messenger ribonucleoprotein particles (mRNP's). A portion of the mRNP's was released from the column by lowering the ionic strength. Other portions were eluted stepwise in buffer containing 50% and 90% formamide and in 0.1-N NaOH. Identical elution patterns were observed whether monitoring incorporated ('31)P-orthophosphate or L- ('32)S methionine, absorbance at 280 nm, or hybridization of ('3)H polyuridylic acid. mRNP's from the first two fractions were analyzed. A bimodal population of particles was detected in sedimentation velocity and sedimentation equilibrium centrifugation. Particles eluted at low ionic strength demonstrated a sedimentation coefficient distribution of 20S-to-80S, with a mean of 55S. Particles eluted in formamide demonstrated a sedimentation coefficient distribution of 20S-to-60S, with a mean of 40S. Particles eluted at low ionic strength displayed two peaks in CsCl centrifugation, with bouyant densities of 1.37 gm/cc and 1.59 gm/cc. Particles eluted in formamide displayed a single peak with a buoyant density of 1.61 gm/cc. Particles eluted at low ionic strength and centrifuged in metrizamide solution formed two bands having bouyant densities of 1.15 gm/cc and 1.30 gm/cc; formamide-eluted particles banded only at the higher density. Mucor 40S ribosomal subunits banded at 1.56 gm/cc and 1.28 gm/cc in CsCl and metrizamide solution respectively. Analysis of mRNP fractions by sodium dodecylsulfate-polyacrylamide gel electrophoresis characterized protein components of these particles which helped explain their biomodal sedimentation behaviour. Each mRNP fraction contained a unique spectrum of proteins not shared by ribosomes or soluble cell proteins. The formamide-eluted particles contained two proteins, whereas the particles eluted at low ionic strength possessed 12 proteins. A 24,000-dalton protein was the predominant form in both. The denser mRNP's were smaller and lacked proteins found in the larger less dense particles. The former had a protein/RNA ratio of 45/55; whereas the later possessed a value of 90/10. Messenger RNA extracted from the mRNP's sedimented between 8S-to-20S.

Matrix Derivatives With Application To Estimation Problems In Multivariate Statistics., Kankanam Gamage. Jinadasa

Electronic Theses and Dissertations

Dept. of Mathematics and Statistics. Paper copy at Leddy Library: Theses & Major Papers - Basement, West Bldg. / Call Number: Thesis1986 .J555. Source: Dissertation Abstracts International, Volume: 62-13, Section: A. Thesis (Ph.D.)--University of Windsor (Canada), 1986.

Studies Of Cu(Ii) Hemocyanin Models., Hilde Paulina. Berends

Electronic Theses and Dissertations

Source: Masters Abstracts International, Volume: 40-07, page: . Thesis (M.Sc.)--University of Windsor (Canada), 1986.

Secondary Sex Ratio In Daphnia Magna (Crustacea, Cladocera)., David Montgomery Barker

Electronic Theses and Dissertations

Source: Masters Abstracts International, Volume: 40-07, page: . Thesis (M.Sc.)--University of Windsor (Canada), 1986.