Digital Commons Network^™

Applications Of Electronic Structure Theory To Problems In Strong-Field Chemistry, Inorganic Chemistry, And Nanomaterial Systems, Jason Anthony Sonk

Wayne State University Dissertations

This dissertation covers research performed on applications of electronic structure theory to various fields of chemistry and is divided into eight chapters. Chapters 2 through 4 describe a series of related works which explore applications of excited state electronic structure methods to problems in strong field chemistry. Chapters 5, 6, and 7 discuss the application of electronic structure theory methods to solving problems in inorganic chemistry. Finally, Chapter 8 looks at an application of electronic structure theory to nanomaterials.

Chapter 2 covers the modeling of electron dynamics of butadiene interacting with a short, intense laser pulse in the absence of …

Metal Complexes Of Non-Heme Ligands: Biological Applications, Jai Prakash

Wayne State University Dissertations

Oxidative inactivation of proteins (carbonic anhydrase–I, trypsin, chymotrypsin and 20S proteasome) by non–heme iron complexes, and glutathionylation of non–heme cobalt complexes mimicking the N5 coordination environment like that of biologically important cofactor cobalamin or B₁₂ (Cbl) are reported. Different non–heme ligand sets or inhibitors were used to inactivate different proteins. Carbonic anhydrase–I (CA–I) and 20S proteasome were inactivated by iron complexes in the presence of O₂ and a reductant (DTT), consistent with a pathway involving the reductive activation of O₂, whereas serine proteases trypsin and chymotrypsin were inactivated by ferryls (single turnover), and by an iron …

Perioperative Experience Of Adolescents, Janean Carter Monahan

Wayne State University Dissertations

Research has shown that preoperative stress is associated with poorer health outcomes in adults and young children, but there is little in the literature about the stress experienced by adolescents. Clinical experience, however, has shown that adolescents behave differently throughout the perioperative experience than either adults or children. For example, common behaviors of adolescent's emerging from anesthesia include combativeness, thrashing, and crying. To promote adolescent health and to provide adolescents with appropriate interventions that will support a positive surgical outcome, research is needed to discover the perceptions and meanings adolescents attribute to the perioperative experience.

The purpose of this hermeneutic …

"Fine-Tuning" Of Ribosomal Structure And Functions By Pseudouridylation And Rna-Protein Interactions, Jun Jiang

Wayne State University Dissertations

ABSTRACT

"Fine-tuning" of ribosomal structure and functions by pseudouridylation and RNA-protein interactions

by

JUN JIANG

AUGUST 2012

Advisor: Prof. John SantaLucia Jr.

Major: Chemistry (Biochemistry)

Degree: Doctor of Philosophy

Ribosomal structure and functions appear to be "fine-tuned" by pseudouridylation and RNA-protein interactions. Pseudouridylation may promote base stacking interactions by mediating the base stacking between residues on both sides. In the RNA duplex region, this enhanced stacking interaction contributes to stabilization of duplex folding. In the loop region, enhanced stacking in one structural motif may destabilize the conformation of adjacent structural residues. This hypothesis is supported by both UV-melting experiments, where …

Patients' And Caregivers' Inside Perspectives: Living With A Left-Ventricular Assist Device As Destination Therapy, Linda Marcuccilli

Wayne State University Dissertations

Left-ventricular assist devices (LVADs) have improved the quality of life for many patients with advanced heart failure. Past research focused on technology issues and survival rates, but patients' and caregivers' perspectives of living with an LVAD as a destination therapy (e.g., permanent alternative to transplant) was not explored. Roy's adaptation model provided a framework to guide an understanding of how participants adjusted and accepted living with destination therapy. A hermeneutic-phenomenology as described by van Manen was used to explore and describe the essence of destination therapy from patients' and caregivers' perspectives in order to understand the meaning of this experience. …

State-Resolved Slice Imaging Of Photochemical Dynamics, Herath Mudiyans Nuradhika Herath

Wayne State University Dissertations

This dissertation focuses on the understanding the unimolecular photochemistry and dynamics

utilizing state-resolved slice imaging approach combined with the quantum-state selective

spectroscopy technique called resonance enhanced multi photon ionization (REMPI)

method. This powerful technique allows selecting the initial quantum states of the reactants

and determining the nal quantum states, energy, the orientation and alignments of

the products. In the investigations of photodissociation dynamics of acetone at 230 nm,

a bimodal distribution for the resulting CO photoproduct is identied. This observation

indicated the presence of unimolecular dissociation mechanism analogues to the roaming

dynamics reported in formaldehyde photodissociation. Moreover, another type of …

Measurement Of Branching Factions Of D Meson With Final State Including Kk, Peng Zhou

Wayne State University Dissertations

Using 818pb−1 of CLEO-c data at √s =3770 MeV, we measure precisely the branching ratios for four D+ → K+K−X hadronic channels. Our measurement improves the errors of B(D+ → K+K−π+π0) and B(D+ → φπ+π0) by factors of 10 respectively. We report first observation of the decay D+ → φρ+, as well as branching ratio measurements for two rare decays.

Nodal Geometry Of Eigenfunctions On Smooth Manifolds And Hardy-Littlewood-Sobolev Inequalities On The Heisenberg Group, Xiaolong Han

Wayne State University Dissertations

Part I: Let (M,g) be a n dimensional smooth, compact, and connected Riemannian manifold without boundary, consider the partial differential equation on M:

-Δu=Λu,

in which Δ is the Laplace-Beltrami operator. That is, u is an eigenfunction with eigenvalue Λ. We analyze the asymptotic behavior of eigenfunctions as Λ go to ∞ (i.e., limit of high energy states) in terms of the following aspects.

(1) Local and global properties of eigenfunctions, including several crucial estimates for further investigation.

(2) Write the nodal set of u as N={u=0}, estimate the size of N using Hausdorff measure. Particularly, surrounding the conjecture that …

An Examination Of Midwest Community College Presidents' Leadership Styles, Marvin Jones Jacobs

Wayne State University Dissertations

This study examined the leadership styles of community college presidents and chancellors in the Midwestern part of the United States on leadership styles they embraced based on their personal characteristics, age, gender, education and experience in higher education, location, and other prevailing personal characteristics. The three leadership styles measured in the study were transformational, transactional and passive avoidant. Three research questions were posed for the study. A total of 176 presidents in five states were sent surveys. Of this number, 82 (46.6%) completed and returned the MLQ questionnaire and the investigator-developed demographic survey. Survey findings suggested participating presidents appeared to …

Use Of Methionine Sulfoximine To Dissect The Role Of Glutamine Synthetase And Glutamine In Progression Of Acute Liver Failure, Amruta Anil Jambekar

Wayne State University Dissertations

Methionine sulfoximine (MSO) is a modified amino acid and a well characterized irreversible inhibitor of glutamine synthetase (GS) enzyme. Glutamine is synthesized by GS enzyme and it is the most abundant amino acid in the body. Glutamine is required by immune cells for generation, propagation and maintenance of an immune response.

To induce acute liver failure (ALF) in mice, animals were given intraperitoneal injections of E. coli lipopolysaccharides (LPS) and sugar D-galactosamine (D-GalN). When these animals were pretreated with MSO, 80% of the animals were completely rescued from liver failure. Moreover, when we characterized the immune response generated during ALF …

The Methuselah Family Of G Protein Coupled Receptors, Meghna V. Patel

Wayne State University Dissertations

G protein-coupled receptors (GPCRs) are one of the largest class of transmembrane signaling proteins that regulate essential developmental and physiological processes in a cell. GPCR success is illustrated by their abundance across both invertebrate and vertebrate genomes. Phylogenetic analyses show that GPCR families have undergone a lot of gene gain and loss during insect evolution. In Drosophila melanogaster, the fifteen Methuselah/Methuselah-like (Mth/Mthl) genes are in fact an insect specific family of GPCRs. In our study, we conducted a phylogenetic analysis using receptor sequences from five Drosophila species and two related insects, including Tribolium and Anopheles to examine the evolution of …

Bankruptcy And Politics: A Framework For Bankruptcy Policymaking In The United States Congress And Courts, Kevin Mandell Ball

Wayne State University Dissertations

The Bankruptcy Abuse Prevention and Consumer Protection Act of 2005 was enacted amid much controversy and was considered by many observers to mark a major change in the direction of U.S. bankruptcy policy. The dissertation uses the law's passage as a vehicle to develop an explicit model of the integrated role of Congress, issue networks, and the courts in making policy. Following Baumgartner and Jones (1993), Sabatier (1988), and others, the dissertation tracks bankruptcy policymaking and implementation over a seventy-five year period to demonstrate that policy is made in three distinct venues: Congress; a policy community made up of lawyers, …

The Role Of Cad,Flash And Fam129b In Cancer Cell Survial And Apoptosis, Song Chen

Wayne State University Dissertations

Apoptosis is a normal process in the human body. However, apoptosis is desregulated in cancer cells. Most cancer cells gain resistance to apoptosis, leading to uncontrolled proliferation. In this dissertation, we identified three proteins, associated with apoptosis pathway. 1) CAD, a large multifunctional complex that is invariably elevated in tumor cells, 2) FLASH, a large protein with multiple growth related functions and 3) FAM129B. We demonstrate that CAD could interact with FLASH by using yeast two hybrid, co-immunopreciptation and fluorescence microscopy. In addition, functional analysis using siRNA technology further indicated that CAD could co-operate with FLASH and play roles in …

A Role For Reactive Oxygen Species In Photodynamic Therapy, Michael Price

Wayne State University Dissertations

Photodynamic therapy (PDT) is based on the ability of certain photosensitizing agents to selectively localize in neoplastic cells and their vasculature. Subsequent irradiation at a wavelength corresponding to a photosensitizer absorbance band excites the photosensitizer molecules, leading to energy transfer reactions and fluorescence. It was initially concluded that the phototoxic effect occurred when energy from the excited state of the photosensitizer was transferred to dissolved oxygen to form singlet oxygen. This product has a very brief half-life and will cause cellular damage only in the immediate vicinity of its formation. But an excited-state photosensitizer can also interact with oxygen to …

The Effect Of Grief And Loss Training For Student Counselors On Grief Counseling Comfort Level In Two Educational Settings, Selin Sertgoz

Wayne State University Dissertations

Loss, whether death or non-death related, is a life-changing experience. When people grieve over their significant loss, they go through a multitude of emotional, behavioral, physiological, and cognitive changes that can be disruptive at times. Counselors report encountering various loss-related issues with their clients, yet novice counselors in particular do not feel comfortable dealing with these issues. Therefore, the purpose of this study was to develop a grief and loss training program from a Rational Emotive Behavioral Theory perspective for counselors to improve their comfort level in grief counseling.

The literature highlights the increasing trend of online training in higher …

Location Privacy In Emerging Network-Based Applications, Yong Xi

Wayne State University Dissertations

With the wide spread of computer systems and networks, privacy has become an issue that increasingly attracts attention. In wireless sensor networks, the location of an event source may be subject to unintentional disclosure through traffic analysis by the attacker. In vehicular networks, authentication leaves a trail to tie a driver to a sequence of time and space coordinates. In a cloud-based navigation system, the location information of a sensitive itinerary is disclosed. Those scenarios have shown that privacy protection is a far-reaching problem that could span many different aspects of a computer/network system, especially on a diversified landscape of …

Effective Semantic-Based Keyword Search Over Relational Databases For Knowledge Discovery, Sina Fakhraee

Wayne State University Dissertations

Keyword-based search has been popularized by Internet web search engines such as Google which is the most commonly used search engine to locate the information on the web. On the other hand while traditional database management systems offer powerful query languages such as SQL, they do not provide keyword-based search similar to the one provided by web search engines. The current amount of text data in relational databases is massive and is growing fast. This increases the importance and need for non-technical users to be able to search for such information using simple keyword search just as how they would …

An Examination Of The Impact Of Early Retirement Incentives On School District Financial Health, Michael Jon Dean

Wayne State University Dissertations

The combination of state controlled school funding in Michigan, rising employee costs and shrinking school enrollments have caused school districts to seek a variety of cost control measures. One of the measures frequently supported by both school districts and employee unions is the use of Early Retirement Incentives (ERI) to incentivize teachers to separate from the school district via a cash payment.

The purpose of this study was to analyze how offering or not-offering an ERI impacted on the financial health of a school districts in the State of Michigan. Selected school districts in Michigan were surveyed regarding any ERI's …

This Fact Which Is Not One: Differential Poetics In Transatlantic American Modernism, Sarah Ruddy

Wayne State University Dissertations

This dissertation proposes that the literary fact, first discussed by Jurij Tynajnov in his 1924 essay "The Literary Fact," and later in "On Literary Evolution" (1929), names an intersection of literary formalism and social representation central to experimental modernist texts in the twentieth century. The poetics of literary fact that I propose finds its basis in Russian Formalist and Frankfurt School theory and reflects several important twentieth century social moments to illustrate how historical and social facts seek poetic form. In my use of the term, "fact" is the materiality of history as it moves from the social world, carrying …

Molecular Mechanisms Of Strain-Stimulated Intestinal Epithelial Cell Differentiation And Migration, Lisi Yuan

Wayne State University Dissertations

The intestinal epithelium is subjected to repetitive deformation during normal gut function by peristalsis and villous motility. In vitro, cyclic strain promotes intestinal epithelial proliferation and induces an absorptive phenotype characterized by increased dipeptidyl dipeptidase (DPPIV) expression. Schlafen 3 is a novel gene recently associated with cellular differentiation. We sought to evaluate whether Schlafen 3 mediates the effects of strain on the differentiation of IEC-6 intestinal epithelial cells in the absence or presence of cyclic strain. Strain increased Schlafen 3 mRNA and protein. In cells transfected with a control non-targeting siRNA, strain increased DPPIV specific activity. However, Schlafen

3 reduction …

The Relationship Between Faculty's Pedagogical Content Knowledge And Students' Knowledge About Diversity In Online Courses, Mitali Chaudhery

Wayne State University Dissertations

The purpose of this proposed study will be to examine the relationship between faculty's pedagogical content knowledge and the design of online curriculum to teach students about diversity in a higher education environment. One hundred twenty-seven faculty teaching online courses at a Midwestern state will be selected on non-random sampling to participate in this study. Two main measures will be used to collected data: (1) Faculty Demographic and Background Survey, and (2) Diversity Teaching survey. The Diversity Teaching Survey was broken into 3 subscales that will examine the following: (1) Faculty's Pedagogical Knowledge and Skills, (2) Online Curriculum Design, and …

The Proton-Coupled Folate Transporter: Biology And Therapeutic Applications To Cancer, Sita Desmoulin

Wayne State University Dissertations

Folates are essential cofactors of tumor cell proliferation and survival required for nucleotide biosynthesis and amino acid metabolism. In cancer therapy, inhibition of folate-dependent metabolic pathways has been achieved through the use of antifolates. Unfortunately, the efficacy of many clinically approved antifolates is limited by a lack of tumor selectivity. Facilitative transport of folates into mammalian cells is achieved by the reduced folate carrier (RFC) and proton-coupled folate transporter (PCFT). As PCFT is a folate-proton symporter with an acidic pH optimum, PCFT may provide a mechanism for targeting cytotoxic antifolates to tumors, based on their acidic microenvironments. To establish the …

Epistemic Communities And Regional Governance: Policy Development In Municipal Finance Reform, Shanthi Karuppusamy

Wayne State University Dissertations

In recent times, the US has undergone significant changes in how regional governance is conceptualized and the focus has shifted from government to governance and from governmental consolidation to problem solving (Barnes and Foster, 2011). Policy makers' quest for interjurisdictional responses to the financial crisis and the recession has rekindled interest in the topic of regional governance. However, the economic, social and technical changes of the recent decades, which have now assimilated in US urban regions, fundamentally challenge existing dominant ways of thinking about regional governance and call for more useful analytic frameworks (Bollens, 1997; Barnes and Foster, 2011). This …

Estimating The Efficiency Of Michigan's Rural And Urban Public School Districts, Rita Ann Maranowski

Wayne State University Dissertations

This study examined student achievement in Michigan public school districts to determine if rural school districts are demonstrating greater financial efficiency by producing higher levels of student achievement than school districts in other geographic locations with similar socioeconomics. Three models were developed using multiple regression analysis of student achievement for high school graduation rates and student proficiency rates for eleventh grade students in mathematics and English language arts as reported from the Michigan Merit Examination results. These models compared student achievement by geographic location which included a selection of 10 independent variables and a sample size of 496 Michigan public …

Characterization Of Intracellular Interactions Between Dengue Virus And Host Proteins, Dumrong Mairiang

Wayne State University Dissertations

Dengue virus is the causative agent of dengue fever, dengue hemorrhagic fever and dengue shock syndrome. About two-fifths of world population live in areas where dengue is prevalent, leading to high levels of morbidity and mortality in many areas. Currently there are no vaccines or effective treatments. The virus is transmitted from one person to another by the yellow fever mosquito, Aedes aegypti. The genome of dengue virus encodes only ten proteins implying that the virus needs to interact with and utilize several host proteins for replication. In this project, I used high-throughput yeast two-hybrid screening to identify mosquito and …

Identification Of Transcriptional Mechanisms Downstream Of Nf1 Gene Defeciency In Malignant Peripheral Nerve Sheath Tumors, Daochun Sun

Wayne State University Dissertations

Malignant peripheral nerve sheath tumor (MPNST) is a type of soft tissue sarcoma that occurs in carriers of mutations in the neurofibromatosis type I gene (Nf1) as well as sporadically. Plexiform neurofibromas in NF1 patients have a significant risk of developing into MPNSTs leading to increased morbidity and mortality from this syndrome. Surgery is the primary intervention but it is not always effective due to the tendency of MPNSTs to infiltrate the surrounding tissue or grow in an inoperable location. Neurofibromin, the protein coded by the Nf1 gene, functions as a GTPase activating protein (GAP) whose mutation leads to constitutive …

Examining Autoimmune Sequelae During Cancer Immunotherapy In A Combined Autoimmune Thyroiditis And Tumor Mouse Model, Suresh Kari

Wayne State University Dissertations

Some clinical trials of immunotherapeutic agents against cancers have resulted in the development of autoimmune sequelae, including autoimmune thyroiditis. It has been suggested that the immunotherapy resulted in an alteration in the immunoregulatory mechanism(s). Using experimental autoimmune thyroiditis (EAT), a mouse model of Hashimoto's thyroiditis (HT), the importance of regulatory T cells (Tregs) has been well established; inhibition of Tregs leads to enhanced thyroiditis with repeated doses of mouse thyroglobulin (mTg) without adjuvant. To simulate cancer patients, we developed a combined EAT and tumor model in our EAT-susceptible mice (CBA/J). In first establishing the tumor model, we found Treg depletion …

Longitudinal Outcomes Of Distortion Product Otoacoustic Emissions And Wideband Reflectance In Infants, Virginia Ramachandran

Wayne State University Dissertations

Clinical practice has shown that some infants are born with, or develop a temporary conductive hearing loss characterized by the absence of measurable otoacoustic emissions (OAEs) but normal sensorineural hearing. This transient situation interferes with the process of universal newborn hearing screening and identification.

The purpose of this prospective, longitudinal study was to describe outcomes of distortion product OAE (DPOAE) screening in infants at birth, and one, four, eight, and twelve weeks of age. In addition, wideband reflectance (WBR) measures, which have the potential to help characterize outer-ear canal and middle-ear function, were examined to determine their potential utility in …

Robustness Of The Achievable Benchmark Of Care Method, Jeff Arthur Capobianco

Wayne State University Dissertations

The Achievable Benchmark of Care Method is a process of care performance improvement measurement approach for identifying top performing healthcare providers. The purpose of this study was to investigate the robustness of the method. This was achieved by comparing the robustness of the standard ABC method, which uses the mean to calculate the benchmark, to versions of the ABC method where the mean was replaced with either a 5%, 10%, or 20% trimmed mean, a 15% Winsorized mean or the one-step Huber ø1.28 calculation. Monte Carlo simulations where conducted using publically available, Medicare process of care data. The mean was …

A New Semantic Similarity Join Method Using Diffusion Maps And Long String Table Attributes, Bilal Hani Hawashin

Wayne State University Dissertations

With the rapid increase of the distributed data sources, and in order to make information integration, there is a need to combine the information that refers to the same entity from different sources. However, there are no global conventions that control the format of the data, and it is impractical to impose such global conventions. Also, there could be some spelling errors in the data as it is entered manually in most of the cases. For such reasons, the need to find and join similar records instead of exact records is important in order to integrate the data. Most of …