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Molecular Mechanisms Of Pressure-Stimulated Cancer Cell Signaling, Christina Downey
Molecular Mechanisms Of Pressure-Stimulated Cancer Cell Signaling, Christina Downey
Wayne State University Dissertations
ABSTRACT
MOLECULAR MECHANISMS OF PRESSURE-STIMULATED CANCER CELL SIGNALING
by
CHRISTINA DOWNEY
June 2010
Advisor: Dr. Marc Basson, MD, PhD
Major: Cancer Biology
Degree: Doctor of Philosophy
Increased extracellular pressure stimulates cancer cell adhesion by a mechanism that is dependent upon beta-1-integrin activation, an intact cytoskeleton, and FAK and Src activation. By a different mechanism, increased extracellular pressure modulates cancer cell proliferation in a manner that is regulated by protein kinase C, but not Src or an intact cytoskeleton. Previous studies from our laboratory have shown that paxillin is a necessary mediator in the pathway by which pressure stimulates adhesion; however, …
Quantification Of Vascular Parametric Indices Using Dynamic Contrast-Enhanced Magnetic Resonance Imaging, Areen Khaled Al.Bashir
Quantification Of Vascular Parametric Indices Using Dynamic Contrast-Enhanced Magnetic Resonance Imaging, Areen Khaled Al.Bashir
Wayne State University Dissertations
Dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) is a non-invasive method used to evaluate the biological activity in early clinical trials of novel drugs targeting the tumor vasculature using gadolinium-DTPA (Gd) as a contrast agent. However, it has some limitations, such as reproducibility, data acquisition times, the presence of noise, extracting contrast concentration, estimating T1 relaxation and estimating pharmacokinetic parameters.
In this work, a new approach to used fixed T1(0) which provides more reproducible DCE results has been introduced. Using this new algorithm to quantify the vascular changes in DCE-MRI, a pre-clinical renal cell carcinoma (RCC) tumor model was used to …
Regulatory And Functional Aspects Of Foxo3a Transcription Factor And Their Implications In Prostate Cancer, Melissa Elise Dobson
Regulatory And Functional Aspects Of Foxo3a Transcription Factor And Their Implications In Prostate Cancer, Melissa Elise Dobson
Wayne State University Dissertations
The P13K/Akt pathway is a critical mediator of growth factor signaling involving many cellular functions. The deregulation of this pathway has been shown to be involved in the development of various cancers. One of the main targets of this pathway is FoxO3a, a transcription factor whose target genes are involved in important cellular processes such as apoptosis, cell cycle control, and glucose metabolism. FoxO3a is regulated by various post translational modifications including acetylation, ubiquitination and phosphorylation. The transcription factor is directly phosphorylated by Akt on 3 residues: Threonine 32, Serine 253 and Serine 315. Phosphorylation by Akt generates binding sites …