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Development Of Scaffold Architectures And Heterotypic Cell Systems For Hepatocyte Transplantation, Dalia Alzebdeh
Development Of Scaffold Architectures And Heterotypic Cell Systems For Hepatocyte Transplantation, Dalia Alzebdeh
Wayne State University Dissertations
In vitro assembly of functional liver tissue is needed to enable the transplantation of tissue-engineered livers. In addition, there is an increasing demand for in vitro models that replicate complex events occurring in the liver. However, tissue engineering of sizable implantable liver systems is currently limited by the difficulty of assembling three dimensional hepatocyte cultures of a useful size, while maintaining full cell viability, an issue which is closely related to the high metabolic rate of hepatocytes. In this study, we first compared two designs of highly porous chitosan-heparin scaffolds seeded with hepatocytes in dynamic perfusion bioreactor systems. The aim …
Reinforced Chitosan-Based Heart Valve Scaffold And Utility Of Bone Marrow-Derived Mesenchymal Stem Cells For Cardiovascular Tissue Engineering, Mohammad Z. Albanna
Reinforced Chitosan-Based Heart Valve Scaffold And Utility Of Bone Marrow-Derived Mesenchymal Stem Cells For Cardiovascular Tissue Engineering, Mohammad Z. Albanna
Wayne State University Dissertations
Recent research has demonstrated a strong correlation between the differentiation profile of mesenchymal stem cells (MSCs) and scaffold stiffness. Chitosan is being widely studied for tissue engineering applications due to its biocompatibility and biodegradability. However, its use in load-bearing applications is limited due to moderate to low mechanical properties. In this study, we investigated the effectiveness of a fiber reinforcement method for enhancing the mechanical properties of chitosan scaffolds. Chitosan fibers were fabricated using a solution extrusion and neutralization method and incorporated into porous chitosan scaffolds. The effects of different fiber/scaffold mass ratios, fiber mechanical properties and fiber lengths on …