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Theses/Dissertations

Master's Theses

Biology

2016

Chloroplast DNA

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Determination Of Evolutionary History Of Big Bluestem Populations Through Chloroplast Dna Analysis, Tej Man Tamang Jul 2016

Determination Of Evolutionary History Of Big Bluestem Populations Through Chloroplast Dna Analysis, Tej Man Tamang

Master's Theses

Andropogon gerardii Vitman (big bluestem) is one of the most dominant and widely distributed grasses of the North American prairie. It is widely used in restoration projects for the recovery of grassland ecosystems. A. gerardii demonstrates genetic and adaptive variation among populations across the prairie. With the objective to understand the evolutionary relationship between the A. gerardii populations, two noncoding chloroplast DNA (cpDNA) spacers (rpl32-trnL(UAG) and trnQ(UUG)-rps16) were studied. Similarly, genetic differentiation among the populations was also calculated based on the spacers. The trnQ(UUG)-rps16 spacer had more polymorphic sites than the rpl32-trnL(UAG) spacer. A phylogenetic tree based on combined cpDNA …