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Gut Microbiota Of Migratory Passerines At Stopover, William Bagley Lewis Dec 2015

Gut Microbiota Of Migratory Passerines At Stopover, William Bagley Lewis

Master's Theses

Although the gut microbiota provides many beneficial functions to animal hosts, relatively little is known about the gut microbiota of passerines. It is likely that gut microbes are especially important during the migratory phase; however gut atrophy experienced during prolonged migratory flight may cause disruptions of the stable microbiota. Fecal samples were collected from several species of passerine after crossing the Gulf of Mexico during spring migration and before crossing during fall, and microbiota communities were analyzed using next-generation sequencing. Despite showing large inter-individual variation, a core microbiota composed largely of Firmicutes and Proteobacteria was identified in all birds. Microbiota …


The Vascular Flora Of Wayne County, Mississippi, Daniel M. Mcnair Dec 2015

The Vascular Flora Of Wayne County, Mississippi, Daniel M. Mcnair

Master's Theses

The vascular flora of Wayne County, Mississippi, was surveyed from 2013–2015, and the species of native and naturalized plants are listed. In total, 1,135 species were identified from field collections, previously collected herbarium specimens, and field observations. These belong to 556 genera and 165 families. Four of these species represent first recorded occurrences in the state of Mississippi: Blyxa aubertii Rich., Gomphrena serrata Pav. ex Moq., Isoetes valida (Engelm.) Clute, and Polypogon interruptus Kunth. GPS coordinates are given for important collection sites representing the diversity of plant communities in the county. All voucher specimens from the survey are deposited in …


Maternal Effects And Offspring Behavior: Potential Contributors To The Lack Of Recruitment In Mississippi Gopher Tortoises (Gopherus Polyphemus), Aaron L. Holbrook Dec 2015

Maternal Effects And Offspring Behavior: Potential Contributors To The Lack Of Recruitment In Mississippi Gopher Tortoises (Gopherus Polyphemus), Aaron L. Holbrook

Master's Theses

Federally threatened in Mississippi, gopher tortoise (Gopherus polyphemus) populations within the state have strongly variable recruitment and are generally in decline. Hatching success is significantly lower in Mississippi than in any other part of the species’ range , and most hatchlings die within the first year. There are few refuges where survival and hatching success is high. Here I compare two populations that differ in recruitment and offspring survival for differences in corticosterone. Corticosterone is a hormone that influences both energy availability and is released in elevated levels during stressful events, like living in poor quality habitat. Prolonged corticosterone elevation …


High Resolution Melt Analysis Of Samples With Differential Dna Methylation To Identify Tissue Source Of Origin, Stephanie M. Ledgerwood Dec 2015

High Resolution Melt Analysis Of Samples With Differential Dna Methylation To Identify Tissue Source Of Origin, Stephanie M. Ledgerwood

Master's Theses

In Forensic Science casework, identifying the source of a tissue can assist in crime scene reconstruction. Currently, presumptive testing methods to identify tissue type are utilized in crime scene laboratories, but there is a need for a more reliable confirmatory test for tissue type identification. High Resolution Melt (HRM) analysis is an innovative technology that has the potential to determine tissue types through variations in DNA methylation patterns. Recently, DNA methylation patterns have been found to correspond with specific tissue types in particular regions of DNA. Two markers, B_SPTB_03 and DDX4 have been effective in differentiating sperm from other tissue …


Latitudinal Variation In Thermal Tolerance And Preference Of Fundulus Notatus And F. Olivaceus: Implications For Temperature Mediated Reproductive Isolation, Laura Kristin Stewart Aug 2015

Latitudinal Variation In Thermal Tolerance And Preference Of Fundulus Notatus And F. Olivaceus: Implications For Temperature Mediated Reproductive Isolation, Laura Kristin Stewart

Master's Theses

Temperature is one of the most pervasive and influential abiotic factors affecting the life histories of fish, regulating a host of physiological processes and influencing behavioral thermoregulation. Fundulus notatus, the blackstripe topminnow, and F. olivaceus, the blackspotted topminnow, are widely distributed species located in drainages along the northern part of Gulf of Mexico (30°N latitude) and as far north as southern Illinois (39°N latitude). However, F. notatus has a much broader range reaching up into the Great Lakes (43°N latitude). Due to their broad geographical range, these populations inhabit a wide range of thermal regimes, thus …


Naturalists’ Perspectives On The Use Of Mobile Technology During A Nature Hike, Aubin Marishka Radzewicz St. Clair Aug 2015

Naturalists’ Perspectives On The Use Of Mobile Technology During A Nature Hike, Aubin Marishka Radzewicz St. Clair

Master's Theses

Naturalists act as our link between scientific knowledge and the public’s understanding of natural history and conservation efforts. In order for them to succeed, they need access to reference materials as well as up-to-date information (Mankin, Warner, & Anderson, 1999). Incorporating mobile technology (i.e. tablets) into naturalists’ endeavors in natural history and environmental education can be used as supportive and educational tools. My project investigated how newly trained naturalists used tablet technology while leading groups of children on nature hikes. I investigated naturalists’ views on the use of mobile technology as a tool during the hikes. My research was guided …


Elucidating The Molecular Function Of Reprolysin Metalloproteases In Tick-Host-Pathogen Interaction, Jaclyn Bo Williams Aug 2015

Elucidating The Molecular Function Of Reprolysin Metalloproteases In Tick-Host-Pathogen Interaction, Jaclyn Bo Williams

Master's Theses

Ticks are ectoparasites that attach to their host for many days to weeks, feeding to engorgement and continuing their species life cycle. The mechanisms behind the successful feeding and manipulation of host immune responses have been associated with the secretion of their specialized saliva. Tick saliva includes a variety of modulatory molecules that must disrupt and counteract defense mechanisms, including a variety of proteases. Reprolysin Metalloproteases, in the Zn2+ dependent family, is secreted as a proenzyme and is shown to have proteolytic activity, degrading fibrinogen and gelatinase activity, as well as disturbing homeostasis12. Previous work with …


Seasonal Variation In Corticosterone, Stress Responses, And Physiology In The Northern Cardinal Cardinalis Cardinalis, Benjamin Matthew Duckworth May 2015

Seasonal Variation In Corticosterone, Stress Responses, And Physiology In The Northern Cardinal Cardinalis Cardinalis, Benjamin Matthew Duckworth

Master's Theses

Seasonal regulation of the adrenocortical response (e.g., ‘stress response’) appears to be ubiquitous in mid- to high-latitude vertebrates. Northern Cardinals (Cardinalis cardinalis) are a Temperate dwelling passerine of Tropical-descent with a wide species range (tropics to Northern Temperate zones). This species encounters a wide variety of environmental conditions and strong seasonality, however corticosterone (CORT, the main adrenal stress hormone) has not been studied with regards to changes in seasonal levels. I initially analyzed samples from cardinals collected between 2007-2011 from the Lake Thoreau Environmental Research and Educational Center (Hattiesburg, Mississippi, USA). The data suggested seasonal differences of CORT, with higher …


Stressed Induced Changes In Karenia Brevis Ribosomal Rna, David Scott Jayroe May 2015

Stressed Induced Changes In Karenia Brevis Ribosomal Rna, David Scott Jayroe

Master's Theses

Karenia brevis is a toxic marine dinoflagellate that causes harmful algal blooms (HABs), also known as red tides, in the Gulf of Mexico. These blooms are responsible for massive fish kills, shellfish bed contaminations, adverse human health effects, and vast economic loss. For these reasons, extensive research has gone into understanding the mechanisms and dynamics of bloom behavior by studying K. brevis in the field and in the lab. In order to understand higher order bloom behavior and dynamics it is imperative to understand K. brevis at the cellular level. In growing K. brevis in vitro under a variety of …


Impacts Of The Deepwater Horizon Oil Spill On Microbial-Mediated Cellulose Decomposition In Mississippi Gulf Coast Salt Marshes, Jerrid Shawn Boyette May 2015

Impacts Of The Deepwater Horizon Oil Spill On Microbial-Mediated Cellulose Decomposition In Mississippi Gulf Coast Salt Marshes, Jerrid Shawn Boyette

Master's Theses

Field studies were conducted to examine the effects of the Deepwater Horizon oil spill on rates of marsh organic matter decomposition. Decomposition in surface and subsurface marsh sediments was assessed in stands of Spartina alterniflora and Juncus roemerianus in 9 Mississippi Gulf Coast marshes exposed to differing oiling intensities. The cotton strip bioassay technique was used as a proxy for cellulose decomposition. In addition, rates of microbial respiration, fungal biomass (ergosterol) and nutrients (C:N, C:P) of surface sediment cotton strips were also quantified. Subsurface cotton strip decay, as determined by losses in tensile strength, were significantly different among marsh …