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Investigation To Examine The Profile Of Auditory Brainstem And Hearing Thresholds Using Tone Burst Audiometry Brainstem Response In A Preclinical Migraine Model, Megan Huelsing Jan 2023

Investigation To Examine The Profile Of Auditory Brainstem And Hearing Thresholds Using Tone Burst Audiometry Brainstem Response In A Preclinical Migraine Model, Megan Huelsing

MSU Graduate Theses

Migraine is a disabling neurological disease that is characterized by prominent auditory symptoms, including hyperacusis, which is defined as extreme sensitivity to sounds. The goal of this study was to investigate changes in hearing sensitivity and neural changes in the brainstem by measuring neural activity of the auditory brainstem in an established preclinical model of chronic migraine. To induce a chronic migraine state within the trigeminal system, male and female Sprague Dawley rats were subjected to three known human risk factors including neck muscle tension and REM sleep deprivation that promote latent sensitization, and exposure to a pungent odor, which …


Body Condition: Seasonal Effects And Influence On Aggressive Behavior Of Ozark Zigzag Salamanders (Plethodon Angusticlavius), Ayesha Farooq Jan 2023

Body Condition: Seasonal Effects And Influence On Aggressive Behavior Of Ozark Zigzag Salamanders (Plethodon Angusticlavius), Ayesha Farooq

MSU Graduate Theses

Body condition is a measure of the size of energy reserves such as fat and protein, which can influence health and the ability to obtain resources in aggressive encounters. Individuals with better body condition ultimately experience a fitness advantage. Physiological factors such as sex, reproductive condition, and diet can influence body condition of individuals. Body condition could be affected positively or negatively after an animal is kept in captivity for extended periods of time. In the first chapter, I explore whether sex, season (fall and spring), and time in containment at the laboratory influences body condition. In the second chapter, …


Centrifugation Cushion Vs. Spermfilter™: Effects On % Recovery, Holly S. Hopkins Jan 2023

Centrifugation Cushion Vs. Spermfilter™: Effects On % Recovery, Holly S. Hopkins

MSU Graduate Theses

It has been demonstrated that removing seminal plasma and resuspension of the sperm pellet in various diluents will improve the post-storage motility of spermatozoa from some stallions. This typically involves centrifugation. However, a sperm filtering device marketed by Botupharma (Phoenix, AZ) allows seminal plasma removal without a centrifuge. This study compared spermatozoa recovery rates using this device vs. centrifugation with and without a cushion. Thirteen ejaculates were collected from three quarter horse stallions. Prior to treatment, aliquots were diluted with Dulbecco's modified PBS at a dilution rate of 1:1. For the two centrifugation treatments, 12 ml of the diluted semen …