Open Access. Powered by Scholars. Published by Universities.®
- Keyword
-
- 2 (1)
- 3-Butanedione monoxime (1)
- ATPase; actin filaments; intracellular trafficking; Myosin; Snc1; time-lapse images; Vps10 (1)
- Amlodipine (1)
- Biopolymer cytotoxicity (1)
-
- Chitosan nanoparticles (1)
- Degree of quaternization (1)
- Gene therapy (1)
- N+/P- ratio (1)
- Nucleotide excision repair (NER) (1)
- P+/P- ratio (1)
- PDNA transfection (1)
- Post-translational modification (1)
- Rad23 (1)
- Teratogen (1)
- Tetrahymena thermophila (1)
- Triethylammonium-butanamide chloride (1)
- Triethylphosphonium-butanamide chloride (1)
- UV Survivability (1)
- UV resistance (1)
- Ubiquitin (1)
- Ubiquitin proteasome system (UPS) (1)
- Verapamil (1)
- Zebrafish (1)
Articles 1 - 4 of 4
Full-Text Articles in Entire DC Network
Investigating Chitosan Modified With Triethylammonium Butanamide And Triethylphosphonium Butanamide As Non-Viral Gene Delivery Vectors By Examining Cytotoxicity And Transfection Efficiency, Deborah C. Ehie
MSU Graduate Theses
Gene therapy is a very challenging field, especially with new emerging genetic disorders. Chitosan (CS), due to chitosan’s flexibility, biocompatibility, and biodegradability, has been of interest in the world of gene therapy especially as researchers are gravitating towards non-viral vectors due to the problems caused by viral vectors. Nevertheless, there are still issues regarding solubility, cellular uptake of cargos being transported in vitro or in vivo, increased cytotoxicity levels, as well as many other things that prevent chitosan from being an efficient gene delivery agent. Here I present five derivatives of chitosan, which were all modified with either triethylphosphonium …
Elucidating The Developmental Defects In Zebrafish Associated With The Cardiac Drug Verapamil, Blake Stephan Justis
Elucidating The Developmental Defects In Zebrafish Associated With The Cardiac Drug Verapamil, Blake Stephan Justis
MSU Graduate Theses
Birth defects are abnormalities in a developing organism that lead to a malformation in structure or function. Over half of birth defects have no determined cause; however, known causes occur by genetic anomalies, exposure to environmental agents (a.k.a. teratogens), or multifactorial reasons. To explain the unknown causes of birth defects, an area of focus in this study is to identify potential teratogens. Identifying these teratogens, is key to preventing future birth defects. An obvious source of teratogens in pregnant women would be that of pharmaceuticals. Thus, a main goal of this study is to identify drugs that cause birth defects. …
Myosin V-Mediated Cargo Traffic Toward The Trans-Golgi Network And Myosin V Implications In Snc1 Exocytosis, Vy Ngoc Khanh Nguyen
Myosin V-Mediated Cargo Traffic Toward The Trans-Golgi Network And Myosin V Implications In Snc1 Exocytosis, Vy Ngoc Khanh Nguyen
MSU Graduate Theses
Retrieval of cargo proteins from the endosome towards the trans-Golgi network (TGN) is a crucial intracellular process for cellular homeostasis. Its dysfunction is associated with pathogenesis of Alzheimer and Parkinson's diseases. Myosin family proteins are cellular motors walking along actin filaments by utilizing the chemical energy from ATP hydrolysis, known to involve in pleiotropic cellular trafficking pathways. However, the question of whether myosins play a role in the trafficking of Snc1 and Vps10 has not been addressed yet. The present study assesses the potential roles of all five yeast myosins in the recycling of two membrane cargo, Snc1 and …
A Novel Uv Resistance In Rad23-Depleted Tetrahymena Thermophila, Emily M. Schmoll
A Novel Uv Resistance In Rad23-Depleted Tetrahymena Thermophila, Emily M. Schmoll
MSU Graduate Theses
Rad23 is a highly conserved cellular scaffold protein which participates in the nucleotide excision repair pathway and ubiquitin proteasome system. It is hypothesized that the contradictory roles of Rad23 within these two systems, acting to enhance stability or facilitate degradation respectively, could be regulated via post-translational modification of the ubiquitin-like domain of the protein. To this end, a Rad23 somatic knockout cell line was established in Tetrahymena thermophila, with the eventual goal of knocking in a mutant Rad23 protein lacking potential for UbL ubiquitylation. In contrast to the UV-sensitive phenotype observed in similar models, Rad23-depleted Tetrahymena cell lines displayed significantly …