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Body Condition: Seasonal Effects And Influence On Aggressive Behavior Of Ozark Zigzag Salamanders (Plethodon Angusticlavius), Ayesha Farooq
Body Condition: Seasonal Effects And Influence On Aggressive Behavior Of Ozark Zigzag Salamanders (Plethodon Angusticlavius), Ayesha Farooq
MSU Graduate Theses
Body condition is a measure of the size of energy reserves such as fat and protein, which can influence health and the ability to obtain resources in aggressive encounters. Individuals with better body condition ultimately experience a fitness advantage. Physiological factors such as sex, reproductive condition, and diet can influence body condition of individuals. Body condition could be affected positively or negatively after an animal is kept in captivity for extended periods of time. In the first chapter, I explore whether sex, season (fall and spring), and time in containment at the laboratory influences body condition. In the second chapter, …
Examining The Immune Regulation Of Nlrp12 Through Novel Protein Interactions, Catherine Rippe
Examining The Immune Regulation Of Nlrp12 Through Novel Protein Interactions, Catherine Rippe
MSU Graduate Theses
NOD-like receptors (NLRs) are intracellular proteins that play an important role in the regulation of the innate immune response to pathogens. Since being identified, various functions for NOD-like receptor pyrin domain containing 12 (NLRP12) have been suggested. It has been shown to negatively regulate the inflammatory response through canonical and noncanonical NF-kB signaling pathways, control tumorigenesis and gut homeostasis and exacerbate inflammation through the formation of a multi-protein complex called an inflammasome. Due to the varying roles established for NLRP12, the mechanisms by which it functions remain poorly understood. In this study, I sought to confirm a novel protein-protein interaction …
Propagation, Physiology And Biomass Of Giant Cane (Arundinaria Gigantea) For Conservation And Restoration, Sanjeev Sharm
Propagation, Physiology And Biomass Of Giant Cane (Arundinaria Gigantea) For Conservation And Restoration, Sanjeev Sharm
MSU Graduate Theses
Giant cane (Arundinaria gigantea) is a native species to 22 states in the U.S. The species and its ecosystem are considered critically endangered, and the species has been reduced to 2% of its original extent. The species has a long cultural and conservation history. Large canebrakes were commonly found in Missouri in bottomland forests, stream and riverbanks, and margins of lakes. My research goals were to: 1) examine methods for propagation success from field to greenhouse to field; 2) examine the physiology of cane at one of the few current canebrakes, for greenhouse propagated plants, and field planted …
Tissue And Sex-Dependent Regulation Of Innate Immunity And Rna Editing In Mice, Kelsey R. Kendrick
Tissue And Sex-Dependent Regulation Of Innate Immunity And Rna Editing In Mice, Kelsey R. Kendrick
MSU Graduate Theses
Inflammation occurs as a result of insult or infection within the body. Individual cells respond to inflammation by upregulating genes that help mediate the immune response, such as ADAR1. ADAR1 helps regulate the immune response but also catalyzes a process called RNA editing. RNA editing alters the sequence of select mRNAs to alter the encoded proteins. The result is altered function of the encoded protein, which is often beneficial for the cell. Our goal was to determine how inflammation affects the function of ADAR1. Since we know that the effects of inflammation vary between different organs and sexes, we examined …
Cul3 Negatively Regulates Nlrp12-Mediated Inhibition Of The Canonical Nf-Κb Signaling Pathway, Inyeong Lee
Cul3 Negatively Regulates Nlrp12-Mediated Inhibition Of The Canonical Nf-Κb Signaling Pathway, Inyeong Lee
MSU Graduate Theses
Nod-like receptor family pyrin domain-containing protein 12 (NLRP12) is mainly known for its inhibitory function on NF-κB signaling in innate immune cells, and more recently, for its ability to regulate chemokine signaling and ubiquitination of the immune receptor RIG-I. Through a yeast 2-hybrid screen, the Lupfer lab previously discovered that NLRP12 interacts with other ubiquitin-associated proteins including Cullin 3 (CUL3) and RING finger protein 2 (RNF2). This research was conducted to mainly investigate the interaction between NLRP12 and CUL3 in human cells and examine the role in regulating NF-κB signaling. Previously, co-immunoprecipitation, followed by western blot analysis, and confocal microscopy …
Largemouth Bass In The Upper Mississippi River: An Evaluation Of Management Strategies And Understanding Potential Factors Influencing Dynamic Rate Functions, Kylie Beth Sterling
Largemouth Bass In The Upper Mississippi River: An Evaluation Of Management Strategies And Understanding Potential Factors Influencing Dynamic Rate Functions, Kylie Beth Sterling
MSU Graduate Theses
The Upper Mississippi River (UMR) supports ecologically and economically important commercial and recreational fisheries. One recreational fishery in the UMR is the Largemouth Bass fishery. Recreational fisheries can be effectively managed using information on population dynamics, though little is known about Largemouth Bass population dynamics in large river ecosystems. Therefore, the objectives of this study were to 1) evaluate recruitment, growth, and mortality of three Largemouth Bass populations in the UMR, specifically within Pools 4, 8, and 13, and 2) to use those estimates of recruitment, growth and mortality to inform exploitation models to evaluate best management practices for each …
Identification Of A Novel Protein Interaction That Elucidates The Mechanism Of Idiopathic Recurrent Miscarriages In Women With Nlrp2 Mutations, Nayeon Son
MSU Graduate Theses
The protein NOD-Like receptor pyrin domain containing 2 (NLRP2) is one member of a larger family of protein receptors that plays an important role in our innate immune system. In humans, the NLR family consists of 22 proteins. However, only about a half of NLRs’ functions are known, but many are pro-inflammatory, causing inflammation. NLRP2 has been identified to be a maternal effect gene regulating early embryo development in idiopathic recurrent miscarriages. In previous studies, mutations in the NLRP2 gene resulted in genetic maternal imprinting disorders due to NLRP2 regulating DNA methylation. However, the exact mechanisms involved in recurrent miscarriages …
Investigation Of The Neuroprotective Effects Of Grape Seed Extract On Trigeminal Ganglion Primary Cultures, Sophia Rose Antonopoulos
Investigation Of The Neuroprotective Effects Of Grape Seed Extract On Trigeminal Ganglion Primary Cultures, Sophia Rose Antonopoulos
MSU Graduate Theses
Migraine and temporomandibular disorders (TMD) are prevalent, debilitating orofacial pain conditions involving peripheral and central sensitization of the trigeminal system. The pro-inflammatory neuropeptide calcitonin gene-related peptide (CGRP), which is synthesized and secreted from trigeminal ganglion neurons, is implicated in the underlying pathology of migraine and TMD. Secreted CGRP modulates the excitability state of neurons and glial cells that express CGRP receptors. Recent studies from our lab in preclinical models of migraine and TMD have provided evidence that dietary supplementation with a proanthocyanin-enriched grape seed extract (GSE) inhibits trigeminal pain signaling. The effect of GSE was blocked by an antagonist of …
Decomposition And Macroinvertebrate Shredder Colonization Of Autumnal Shed Sycamore Leaves In Mining-Contaminated Streams, Leslie Marie Hatch
Decomposition And Macroinvertebrate Shredder Colonization Of Autumnal Shed Sycamore Leaves In Mining-Contaminated Streams, Leslie Marie Hatch
MSU Graduate Theses
Leaf decomposition in streams is an important ecological function facilitated by bacteria, fungi, and macroinvertebrates. Metal contamination can decrease leaf decomposition rates and reduce macroinvertebrate abundance and diversity. In the current study, I focused on mining-contamination in well-buffered streams in two different Missouri mining districts with varying extents of mining contamination, Big River and Pierson Creek. I measured decomposition rates of sycamore leaves, and abundance and diversity of macroinvertebrate shredders in leaf pack experiments with a full-factorial design to determine the effects of metal contamination of leaves and stream substrates. Comparisons were made between leaf packs upstream and downstream of …
Corbicula Fluminea Effects On Survival, Growth, And Drift Of Juvenile Lampsilis Siliquoidea In Laboratory Exposures, Allison Nicole Sieja
Corbicula Fluminea Effects On Survival, Growth, And Drift Of Juvenile Lampsilis Siliquoidea In Laboratory Exposures, Allison Nicole Sieja
MSU Graduate Theses
The Asian clam, Corbicula fluminea, is an invasive species that is abundant and often co-occurs with native freshwater mussels. Corbicula is widely suspected of having negative effects on native mussels, but few studies have empirically tested this hypothesis. I used laboratory experiments to evaluate how adult Corbicula affected the survivorship, growth, and drift of juvenile Lampsilis siliquoidea. Survival and growth of newly metamorphosed mussels were tested in downwelling flow-through chambers with glass-bead substrate. Treatments were control (no clams), small adult clams, or large adult clams. After 28 days, large clams slightly but significantly reduced the number of juveniles …
An Assessment Of Inp/Zns As Potential Anti-Cancer Therapy: Quantum Dot Treatment Induces Stress On Hela Cells, Victoria Grace Davenport
An Assessment Of Inp/Zns As Potential Anti-Cancer Therapy: Quantum Dot Treatment Induces Stress On Hela Cells, Victoria Grace Davenport
MSU Graduate Theses
Indium Phosphide/Zinc Sulfide (InP/ZnS) quantum dots (QDs) are an emerging option in QD technologies for uses of fluorescent imaging as well as targeted drug and anti-cancer therapies based on their customizable properties. In this study we explored effects of InP/ZnS when treated with HeLa cervical cancer cells. We employed XTT viability assays, reactive oxygen species (ROS) analysis, and apoptosis analysis to better understand cytotoxicity extents at different concentrations of InP/ZnS. In addition, we compared the transcriptome profile from the QDtreated HeLa cells with that of untreated HeLa cells to identify changes to the transcriptome in response to the QD. Intracellular …
Ecological Correlates Of Alligator Snapping Turtle Bite Performance, Ashley Herrin Gagnon
Ecological Correlates Of Alligator Snapping Turtle Bite Performance, Ashley Herrin Gagnon
MSU Graduate Theses
The Alligator Snapping Turtle (Macrochelys temminckii) is one of many turtle species facing conservation challenges. Nearly extirpated in the 1980s, it is currently the subject of a head-start initiative, of which, any lasting behavioral or physiological effects were—until now—unknown! To evaluate the ability of captive-reared individuals to excel in natural habitats, and to foresee any future research or conservation challenges regarding this animal, I explored a suite of variables that influence bite performance and behaviors including captive or free-ranging status, and environmental conditions including body temperature and season. My results indicated that free-ranging M. temminckii outperform those residing …
On The Rocks: Changes In Substrate Alter Antipredator Behavior In Benthic Stream Fish, Sarah White
On The Rocks: Changes In Substrate Alter Antipredator Behavior In Benthic Stream Fish, Sarah White
MSU Graduate Theses
Natural selection should strongly favor characteristics that make prey difficult for predators to distinguish from the background, including both morphological and behavioral crypsis. The Rainbow Darter (Etheostoma caeruleum) is a small, cryptic, benthic fish that inhabits gravel-bottomed streams and is preyed upon by predators such as the benthic Knobfin Sculpin (Cottus immaculatus) and the pelagic Longear Sunfish (Lepomis megalotis). In three experiments, I tested whether the behavior of darters was influenced by the opportunity for crypticity offered by their substrate and by the presence of cues from benthic and pelagic predators. First, darters in …
Effects Of Heavy Metal Pollution On The Antipredator Behavior Of Orangethroat Darters (Etheostoma Spectabile), Caleb S. O'Neal
Effects Of Heavy Metal Pollution On The Antipredator Behavior Of Orangethroat Darters (Etheostoma Spectabile), Caleb S. O'Neal
MSU Graduate Theses
Heavy metal pollution can have numerous negative impacts on stream fishes, including both lethal and sublethal effects. Because of the sensitivity of fishes to toxins, they are excellent environmental indicators of stream and watershed health. The Tri-State Mining District is a Superfund site located in parts of Missouri, Kansas and Oklahoma that offers a good opportunity to study sublethal effects of heavy metal pollutants on fish behavior. I observed the antipredator behavior of Orangethroat Darters (Etheostoma spectabile) from 3 streams that varied in the abundance of heavy metal pollutants. In the lab, darters from the most polluted site …
Spatial Use By Mammals Within Two State Parks In The Ozarks National Scenic Riverways, Benjamin Aaron Smith
Spatial Use By Mammals Within Two State Parks In The Ozarks National Scenic Riverways, Benjamin Aaron Smith
MSU Graduate Theses
A mammalian species inventory with comparisons between sampled sites was conducted via multiple methodologies to document presence of mammals at two Missouri state parks within the Ozark National Scenic Riverways. Camera traps, small mammal traps, acoustic detectors, and mist nets were used to detect species at the parks, and species similarity indices and occupancy analyses were used to discern use of space. A mammalian inventory was compiled for each area of inquiry. Greater diversity was found at the park with more variable habitat types. Bat activity was more in the park with a known hibernaculum, though species specific activity differed …
Fish And Macroinvertebrate Response To Restoration And Conservation Efforts, Madison C. Cogar
Fish And Macroinvertebrate Response To Restoration And Conservation Efforts, Madison C. Cogar
MSU Graduate Theses
Fish and macroinvertebrate response to restoration and conservation efforts varies in regards to the size and structure of the system (e.g. headwater streams in WV versus large rivers such as the Mississippi River). This project reviews fish and macroinvertebrate rebound in treated acid mine drainage (AMD) streams in WV as well as macroinvertebrate drift patterns in the Mississippi and Missouri Rivers. AMD is a product of a chemical reaction resulting in an acidic water outflow from mining sites, which may harm aquatic life. As a response, passive AMD treatment systems have been installed. I tested the effectiveness of remediation by …
Population Structure And Habitat Use Of Bluegill In The Upper Mississippi River, Ethan Allyn Rutledge
Population Structure And Habitat Use Of Bluegill In The Upper Mississippi River, Ethan Allyn Rutledge
MSU Graduate Theses
Fish populations are driven by the dynamic rate functions (i.e., recruitment, growth, and mortality). Knowledge of these vital rates can provide critical information to determine spatiotemporal population-level changes in the system. Therefore, understanding these vital rates are important in the proper management of any fishery. Anthropogenic modifications to the environment have had damaging effects on the organisms within these ecosystems. Specific to Upper Mississippi River fishes, channelization, dams, and loss of floodplain connectivity have all been purported as deleterious. In the face of these modifications, understanding habitat use and vital rates of individual species is needed to help guide management …
The Effects Of Titanium Dioxide Nanoparticles On The Growth And Development Of Sorghum Bicolor (L.) Moenech, Adam Gregory Shoemaker
The Effects Of Titanium Dioxide Nanoparticles On The Growth And Development Of Sorghum Bicolor (L.) Moenech, Adam Gregory Shoemaker
MSU Graduate Theses
Engineered nanoparticles (ENPs) have seen a drastic increase in their use over the past decade in various consumer products. ENPs will therefore enter terrestrial ecosystems and soils with increasing frequencies, yet research into the effects of ENPs on living organisms and crops is greatly lacking. Currently, there is only one major study reported on the effects of a single ENP, silver quantum dots, on Sorghum bicolor, the 5th largest crop in the world. I examined the effects of a commonly used metal oxide nanoparticle, titanium dioxide (TiO2), on the growth and development of sorghum grown in …
Dragonflies And Damselflies (Insecta: Odonata) As Indicators For Riparian Condition In Ozark Spring Streams, Cameron Riley Cheri
Dragonflies And Damselflies (Insecta: Odonata) As Indicators For Riparian Condition In Ozark Spring Streams, Cameron Riley Cheri
MSU Graduate Theses
The Odonata (dragonflies and damselflies) are often ineffectively sampled during standard stream bioassessments in North America. Subsequently, odonates are not frequently regarded as informative taxa for stream assessment, particularly when monitoring the ecological impacts of organic pollution. I hypothesized that stream-dwelling odonates should be more useful bioindicators for the assessment of riparian conditions surrounding streams because vegetation associated with streams is used for oviposition, roosting and to establish breeding territories. I selected twelve Ozark spring streams that satisfied a broad array of riparian conditions for study. I sampled each stream’s odonate and total benthic community along with both instream and …
Effect Of Silver And Cadmium Nanoparticles On Endocytosis And Protein Recycling In Yeast, Lakshmi Sravya Rallabandi
Effect Of Silver And Cadmium Nanoparticles On Endocytosis And Protein Recycling In Yeast, Lakshmi Sravya Rallabandi
MSU Graduate Theses
Endocytosis is a highly regulated process crucial for recycling of plasma membrane proteins and lipids. Derailments in this trafficking pathway pose threat to normal functioning of somatic cells. Engineered nanoparticles are used extensively in industry due to unique physicochemical properties. However, these nanoparticles, at high concentrations are known to create toxic effects on biological tissues. With this recent information, I investigated the potential toxicity of silver nanoparticles (AgNP) and cadmium selenium/zinc sulfide (CdSe/ZnS) quantum dots (QDs) on liquid phase endocytosis pathway in Saccharomyces cerevisiae. My data provided evidence that treatment of yeast cells with AgNP and CdSe/ZnS QDs resulted …
Novel Cyanoximates As An Alternative In Cancer Chemotherapy, Kafayat Aderonke Yusuf
Novel Cyanoximates As An Alternative In Cancer Chemotherapy, Kafayat Aderonke Yusuf
MSU Graduate Theses
Chemotherapy is one of the most effective treatment plans for several cancer types. The recurrent side effects derived from chemotherapy agents have warranted the search for novel chemical compounds with better efficacy and minimal side effects. In line with this idea, I investigated effects of a group of newly synthesized metal based chemical compounds called cyanoximates on HeLa human cancer cells. Cyanoximates used were Pt(DECO)2, Pt(MCO)2, and Pd(DECO)2 along with the chemotherapy drug cisplatin as a positive control. I found that the metal cyanoximates reduced cell viability via apoptosis, and that Pt(DECO)2 was most …
The Effects Of An Applied Phyllosphere-Microbiome On Gas Exchange And Growth Of Soybean Infected With Pseudomonas Syringae: Harnessing The Power Of The Microbiome, Charles Agbavor
MSU Graduate Theses
The microbiome is an important determinant of plant health, growth and resistance to stress. This study was conducted to determine the efficacy of the phyllosphere-microbiome on managing Pseudomonas syringae pv. Glycinea (Psg), gas exchange and growth of Glycine max L. Merrill. A greenhouse study was conducted in the summer of 2019. A field isolated and cultured soybean phyllosphere-microbiome was applied to purposely infected and uninfected soybean. Gas exchange, Psg density, chlorophyll concentrations, and percent nitrogen and carbon in the leaves were measured during the V3-V6 stages of soybean. Not surprisingly, there was a two-fold reduction in copies of Pseudomonas …
Investigation Of The Individual Soybean Root Nodule Microbiome, Parris Mica Mayhood
Investigation Of The Individual Soybean Root Nodule Microbiome, Parris Mica Mayhood
MSU Graduate Theses
Soybean is a valuable crop that establishes a symbiotic association with nitrogen-fixing microorganisms. Previous research suggests members of the genus Bradyrhizobium are the major nitrogen-fixing inhabitants of soybean root nodules. However, results from recent studies identified the presence of other bacteria, so called non-rhizobial endophytes (NREs), inside root nodules. All previous research has pooled nodules of different plants for bacterial community analysis, but this study investigated the presence and relative abundance of rhizobia and NREs within multiple root nodules of a single plant. Three sites were selected on Kendrick Farm in Springfield, MO, where three plants and rhizosphere soil were …
Habitat Selection And Host Detection In The Salamander Mussel, Simpsonaias Ambigua, Eric A. Stegmann
Habitat Selection And Host Detection In The Salamander Mussel, Simpsonaias Ambigua, Eric A. Stegmann
MSU Graduate Theses
The native freshwater mussels, Order Unionida, have suffered many species extinctions and loss of abundance. Two important threats to native mussels are loss of habitat and loss of access to the vertebrate hosts of the parasitic mussel larvae. The Salamander mussel, Simpsonaias ambigua, is a habitat specialist, living under flat rocks. It is often found in direct association with its only known host, the common mudpuppy, Necturus maculosus. This association could result from movement and habitat selection by the mussels themselves. Alternatively, it might result from the deposition of juveniles by a resident host. Habitat selection and host …
Freshwater Turtle Community Composition In Mined Land Strip Pit Lakes And The Effects Of Learned Trap Avoidance On Capture Rates Of Sternotherus Odoratus And Trachemys Scripta, Ethan Craig Hollender
Freshwater Turtle Community Composition In Mined Land Strip Pit Lakes And The Effects Of Learned Trap Avoidance On Capture Rates Of Sternotherus Odoratus And Trachemys Scripta, Ethan Craig Hollender
MSU Graduate Theses
Turtles are among the most threatened groups of organisms on the planet and as such are in need of protected habitat where healthy communities can be maintained. The reclamation of land that was formerly the site of surface strip mining provides a matrix of reclaimed terrestrial landscapes rich with lakes and ponds that have the potential to function as such habitat. To determine the suitability of these habitats for turtle communities, I compared the turtle communities of strip pit lakes and natural lakes in southeastern Kansas. Of the seven species of aquatic turtle I encountered, Common Snapping Turtles (Chelydra …
Pollinator Networks In Established Urban Prairies Compared To Rural Remnant Prairies, Amanda Lynn Coleman
Pollinator Networks In Established Urban Prairies Compared To Rural Remnant Prairies, Amanda Lynn Coleman
MSU Graduate Theses
Prairies support over 800 species of plants, insects, birds, fish, and mammals, even though only 1% of remnant prairies remain in the United States. Importantly, urban prairie “gardens/plots” are gaining popularity for their ecological services. However, it is not known to what extent these small urban prairies can sustain the plant-pollinator interactions that are vital to both the insects and the plants. The goal of my research was to examine plant/pollinator interactions in three urban prairies in southwest Missouri and compare them to rural prairies because rural prairies were predicted to have stronger plant/pollinator networks. Rural units were: Woods Prairie, …
Sequence Analysis Of The Ren1 Genomic Region From The Grapevine ‘Kishmish Vatkana’, Dani Joseph
Sequence Analysis Of The Ren1 Genomic Region From The Grapevine ‘Kishmish Vatkana’, Dani Joseph
MSU Graduate Theses
The REN1 region of the grapevine ‘Kishmish Vatkana’ was mapped as the locus that confers resistance to the economically important disease, grape powdery mildew. The purpose of this work was to extend the nucleotide sequence information of this region. By sequencing a heretofore unknown bacterial artificial chromosome clone, the sequence information of this region was extended by 46,890 nucleotides. Sequencing was performed using the third-generation sequencing method, named Oxford Nanopore Technology (ONT). In order to improve the accuracy of the sequence data, a modified ONT library preparation method was developed. ONT sequencing of a library prepared with the modified protocol …
The Effect Of Metal Composition And Particle Size On Nanostructure-Toxicity In Plants, Natalie Lynn Smith
The Effect Of Metal Composition And Particle Size On Nanostructure-Toxicity In Plants, Natalie Lynn Smith
MSU Graduate Theses
Silver nanoparticles (AgNPs) have consistently been shown to have a detrimental effect on bacteria, fungi, and plants. The interaction of AgNPs with plants has received considerable scientific attention, because it is potentially through plants that these structures can enter the food chain and bioaccumulate in humans and animals. To determine the effects of AgNPs on plants, Arabidopsis thaliana seedlings were chronically exposed to sublethal levels of AgNPs using a standardized method. To gain insight on mechanism of phytotoxicity, the seedlings were exposed to low concentrations of Ag+ (in the form of silver nitrate), AgNPs, or gold nanoparticles (AuNPs). To …
The Effects Of Electrofishing On Different Life Stages Of Ozark And Eastern Hellbenders, Stephanie Kay Morrison
The Effects Of Electrofishing On Different Life Stages Of Ozark And Eastern Hellbenders, Stephanie Kay Morrison
MSU Graduate Theses
Electrofishing, a common method of freshwater fish sampling, has been shown to negatively affect some fish species, but the effects on non-target species, such as hellbenders, have not been well studied. I tested effects of electrofishing on the behavior of several life stages of captivereared Ozark (Cryptobranchus alleganiensis bishopi) and eastern (C. a. alleganiensis) hellbenders. Ozark hellbender eggs were exposed to different voltages in the laboratory, and embryos in higher voltages had higher incidences of twitching during exposures and higher numbers of morphological deformities after exposures. For hatchling Ozark hellbenders, which typically are sedentary, individuals moved more during exposure to …
Mapping A New Disease Resistance Locus In An F1 Progeny Derived From Two Grape Wild Relatives, Gaurab Bhattarai
Mapping A New Disease Resistance Locus In An F1 Progeny Derived From Two Grape Wild Relatives, Gaurab Bhattarai
MSU Graduate Theses
Linkage maps and quantitative trait loci (QTL) analysis have become essential tools for the positional cloning of agronomically important genes and for marker-assisted breeding. In this study, two North American grape species, Vitis rupestris and Vitis riparia, and their 294 F1 progeny were used to construct parental linkage maps and to perform QTL analysis for downy mildew resistance. Single nucleotide polymorphism (SNP) discovery was accomplished using genotyping-by-sequencing (GBS) and resulted in 348,888 SNPs. Of these, 11,063 informative SNP markers (3.17% of the original SNP dataset) were derived after filtering for various quality parameters and missing data. A two-way …