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Detection And Evaluation Of Temperature Effects On Cell Proliferation In Somatic Tissues Of The Eastern Oyster, Crassostrea Virginica, By Flow Cytometry, Fernando Jimenez
Detection And Evaluation Of Temperature Effects On Cell Proliferation In Somatic Tissues Of The Eastern Oyster, Crassostrea Virginica, By Flow Cytometry, Fernando Jimenez
LSU Master's Theses
The goal of this thesis was to evaluate temperature effects on cell proliferation of eastern oyster somatic tissues for the development of an oyster cell line. Understanding the in vivo cell proliferation of an organism is essential for the development of cell culture. Cell proliferation can be measured by identifying nuclear cellular proteins involved in growth regulation and cellular transformation. The primary objectives of this study were to: 1) develop an assay to evaluate cell proliferation, 2) develop an assay to analyze nuclear RNA content, and 3) evaluate temperature effects on cell proliferation in somatic tissues. Proliferating cell nuclear antigen …
Effect Of Estradiol-17b On The Gonadal Developemnt Of Diploid And Triploid Female Eastern Oysters, Roberto Quintana
Effect Of Estradiol-17b On The Gonadal Developemnt Of Diploid And Triploid Female Eastern Oysters, Roberto Quintana
LSU Master's Theses
Declines in annual oyster landings and problems associated with seasonal reduction of oyster meat yields have increased interest for development of techniques to produce oysters with enhanced growth characteristics. Research interest has been focused on developing improved lines by induction of polyploidy. The goal of the present study was to evaluate the enhancement of gonadal development of triploid oysters, by the use of the hormone estradiol 17-â (E2) to produce viable eggs for the development of tetraploid broodstocks. Previous studies with the Pacific oyster, Crassostrea gigas, have shown: (1) the need to use the larger eggs of triploid females to …