Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 1 of 1
Full-Text Articles in Entire DC Network
Regulation Of Na,K-Atpase Activity By Tyrosine Phosphorylation In Lens Cells., Larry Darko Bozulic 1974-
Regulation Of Na,K-Atpase Activity By Tyrosine Phosphorylation In Lens Cells., Larry Darko Bozulic 1974-
Electronic Theses and Dissertations
Na,K-ATPase is essential for the regulation of cytoplasmic Na+ and K+ levels in lens cells. Insufficient Na,K-ATPase activity is associated with cataract formation. Based on earlier studies in which Src-tyrosine kinase inhibitors were found to suppress Na,K-ATPase activity changes that occur in response to either thrombin, endothelin, or dopamine, I hypothesize that regulation of Na,K-ATPase activity might occur through phosphorylation of the Na,K-ATPase a1 (catalytic) subunit by Src-family tyrosine kinases. Here, I tested the influence of Src-kinase family members on tyrosine phosphorylation and Na,K-ATPase activity in membrane material isolated from porcine lens epithelium. Western blot studies indicated the expression of …