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Targeted Gene Replacement To Mimic Familial Hypertrophic Cardiomyopathy In Caenorhabditis Elegans, Craig Alan Almeida

Doctoral Dissertations

The identification in invertebrates of genes homologous to human disease genes provides the possibility to broaden the spectrum of model organisms to include experimental invertebrates. The goal of this study is to test the possibility of using Caenorhabditis elegans as an animal model for the inherited human heart disorder familial hypertrophic cardiomyopathy. In 10-30% of the affected families that have been studied, this disease is caused by mutations in the $\beta$-cardiac myosin heavy chain (MHC) gene. The most common of these mutations results in the replacement of an evolutionarily conserved arginine residue (R403) with glutamine (R403Q). While this information has …

The Purification And Characterization Of An Extrapallial Fluid Protein From The Mollusc, Mytilus Edulis, Stephen Joseph Hattan

Doctoral Dissertations

Shell deposition within the bivalve mollusc Mytilus edulis is regulated by the macromolecules of the extrapallial fluid (EP fluid). These mineralization regulating macromolecules are thought to be responsible for the nucleation, growth regulation and growth cessation of the CaCO$\sb3$ mineral crystals that will inevitable constitute $\geq$95% of the mature shell.

This dissertation presents the results of the purification and characterization of the major EP fluid protein of the bivalve Mytilus edulis. The major EP fluid protein was determined to comprise 56% of the total fluid protein and to consist of 7.25% by weight carbohydrate. The protein was purified though a …

Investigation Of Pd(A)(20)Pd(T)(20) In The Analytical Electrophoresis Apparatus, John Oliver Wooll

Doctoral Dissertations

The analytical electrophoresis apparatus (AEA) is capable of generating and monitoring the electrophoretic migration of macroions. The oligonucleotide pd(A)$\sb{20}\cdot$pd(T)$\sb{20}$ was use as a model to compound to evaluate the range and validity of AEA measurements under a variety of electric fields and solvent conditions. A broad range of electric fields yield consistent, reproducible values. The charge determination from different procedures, steady state electrophoresis (SSE) and electrophoretic mobility, have not been consolidated into a consistent theory but advancements in the scope and understanding of the AEA's potential have been made. The apparent charge from the AEA measured electrophoretic mobility, $\mu$, of …