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Articles 1 - 9 of 9
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Investigation Of Age Related Differences In The Rewiring Of P2-Olfactory Receptor Neurons, Daniel Joseph Galante
Investigation Of Age Related Differences In The Rewiring Of P2-Olfactory Receptor Neurons, Daniel Joseph Galante
Theses and Dissertations
Olfactory receptor neurons (ORNs) maintain the ability to regenerate. These neurons reside in the olfactory epithelium and project axons that connect to the olfactory bulbs. Despite the diffuse distribution of ORNs in the olfactory epithelium, they converge at discrete glomeruli in the olfactory bulb. In the P2 IRES tau-lacZ mouse, the P2 ORN subtype has been previously mapped to two glomeruli, using X-gal staining. To determine if age affects ORN regeneration, left olfactory nerve transections were performed on P2 mice from immature (five-weeks old) and mature (1 6-weeks old) groups. Following recovery, the olfactory bulbs were processed to observe ORN …
Signaling By Protease-Activated Receptors In Gastrointestinal Smooth Muscle, Wimolpak Sriwai
Signaling By Protease-Activated Receptors In Gastrointestinal Smooth Muscle, Wimolpak Sriwai
Theses and Dissertations
In the present study, we have examined the expression of protease-activated receptors (PARS) and characterized their signaling pathways in rabbit gastric muscle cells. Immunoblot analysis revealed expression of PARl and PAR2 but not PAR3 or PAR4 in smooth muscle. The PARl agonist TFLLR activated Gq, G12, and Gi3, but not Gil, Gi2, G13, Gs or Gz, whereas the PAR2 agonist SLIGRL activated Gq, G13, Gil, and Gi2, but not Gi3, G12, Gs, or Gz. Both PARl and PAR2 agonists stimulated PI hydrolysis and Rho kinase activity and inhibited cAMP formation. PAR1-stimulated PI hydrolysis was abolished in cells expressing Gαq minigene, …
Role Of Rok And Pkc In Permeabilized Rabbit Femoral Artery, Lyndsay Jacquelyn Clelland
Role Of Rok And Pkc In Permeabilized Rabbit Femoral Artery, Lyndsay Jacquelyn Clelland
Theses and Dissertations
Discoveries made with KCl-induced contractions have elucidated the more complex reactions involved in GPCRs signaling; once the mechanisms of smooth muscle Ca2+ sensitization and desensitization are fully understood, then the development of advanced treatments for vascular disorders such as hypertension, cerebral and coronary vasospasm, and vascular hyporeactivity following hemorrhagic shock may be possible. Studies have shown that KCl-induced contractions induce Ca2+-sensitization. Therefore, we tested the hypothesis that KCl induced Ca2+-sensitization is due to ROK activation by the increase in [Ca2+]i. To test this hypothesis, rabbit femoral arteries were permeabilized with 20µg/ml α-toxin and 1% Triton X-100 and subjected to different …
Effects Of Cxcl8 Overexpression On Tumor Cell Proliferation And Migration In An Hnscc Cell Model, Emil Paul Christofakis
Effects Of Cxcl8 Overexpression On Tumor Cell Proliferation And Migration In An Hnscc Cell Model, Emil Paul Christofakis
Theses and Dissertations
Head and neck squamous cell carcinoma is the 6th most common malignancyworldwide. Recently, a link between cancer and inflammation has been found. Mediatingthis relationship are the chemotactic cytokines known as chemokines. CXCL8 (Interleukin-8), a CXC ELR+ Chemokine mainly responsible for neutrophil chemoattraction, has beenimplicated in increased tumor proliferation, migration and angiogenesis. The current studytests the effects of CXCL8 on the tumor proliferation and metastasis. By genetically modifying cells to knockdown or overexpress the CXCL8 gene we tested its biological rolein head and neck cancer progression. Overexpression of CXCL8 in HN4 tumor cells withlow endogenous CXCL8 levels was found to increase …
Induction Of Heme Oxygenase By A Carbon Monoxide-Releasing Molecule, Robert Andrew Kulina
Induction Of Heme Oxygenase By A Carbon Monoxide-Releasing Molecule, Robert Andrew Kulina
Theses and Dissertations
We have recently demonstrated that heme oxygenase is expressed in both healing wounds and in pressure ulcers. Heme oxygenase has been shown to have important cytoprotective functions in myocardial ischemia-reperfusion injury and organ allograft survival. The cytoprotective effects of heme oxygenase are multifactorial. Besides reducing levels of pro-oxidant heme, heme oxygenase products (bilirubin, carbon monoxide, and iron) have been demonstrated to possess anti-oxidant, anti-inflammatory, anti-apoptotic, and anti-proliferative properties. These properties make heme oxygenase an attractive therapeutic target for the prevention and treatment of chronic wounds. The purpose of this study was two-fold: evaluate the effects of carbon monoxide (CO) on …
The Expression Of Neutrophil Products, Myeloperoxidase And Matrix Metalloproteinase 8, In Systemic Vasculature Of Obese And Preeclamptic Women, Juhi Shukla
Theses and Dissertations
Evidence shows the activation of neutrophils in the systemic vasculature of obese and preeclamptic women. In this study, I evaluated whether expression of neutrophil products, myeloperoxidase (MPO) and matrix metalloproteinase 8 (MMPS), was associated with neutrophil infiltration in systemic vascular tissue of obese and preeclamptic women. I tested my hypotheses by using immunohistochemical studies to look at the expression of MPO and MMP8 in the vasculature of obese and preeclamptic women. There was a significantly greater expression of MPO and MMP8 in the vasculature of preeclamptic women as compared to normal pregnant and normalnonpregnant women. The vasculature of obese women …
Electrospun Blends Of Polydioxanone And Fibrinogen For Urological Applications, Joshua Ford Grant
Electrospun Blends Of Polydioxanone And Fibrinogen For Urological Applications, Joshua Ford Grant
Theses and Dissertations
The need for tissue and organ replacements cannot be satisfied by autograft and allografts alone. The purpose of this study was to investigate the feasibility of electrospinning a blend of polydioxanone and fibrinogen to produce an engineered tissue scaffold. Fiber diameter and pore size of blends were characterized, as well as mechanical strength. Cell proliferation assays for 1 and 7 day cultures were preformed, and a histological evaluation was performed to determine how favorable the various blends were to cell infiltration and proliferation. Some ratios of blends were identified that contained both acceptable mechanical properties and properties that facilitated cell …
Genetic Variations In Interferon-Induced Genes And Hcv Recurrence After Liver Transplantation, Benjamin Cameron Whitehill
Genetic Variations In Interferon-Induced Genes And Hcv Recurrence After Liver Transplantation, Benjamin Cameron Whitehill
Theses and Dissertations
Hepatitis C Virus (HCV) infection represents a worldwide pandemic and is currently the leading cause of cirrhosis and liver transplantation. After transplantation recurrence is almost universal with 96% of patients testing positive for viral RNA and exhibiting histological evidence of infection within the first year. Type I interferons (IFN) and interferon inducible genes are responsible for the innate antiviral state and single nucleotide polymorphisms (SNPs) within these genes may affect the patients ability to respond post-transplantation. We hypothesize the elucidation of associations between SNPs in Type-I Interferon and Interferon inducible genes and HCV recurrence post-liver transplantation might help to identify …
Divergent Roles Of Pi3k And Akt In Rapamycin-Induced Cardioprotection Against Ischemia-Reperfusion Injury, Shivani Kirit Desai
Divergent Roles Of Pi3k And Akt In Rapamycin-Induced Cardioprotection Against Ischemia-Reperfusion Injury, Shivani Kirit Desai
Theses and Dissertations
Coronary heart disease (CHD) is one of the leading causes of death every year with nearly three-fourths of all deaths caused by the disease. The challenge scientists are facing today is discovering new drugs to protect the heart against cellular damage caused by ischemia-reperfusion injury (I-R injury). Rapamycin is one such drug that has been shown to protect the heart against ischemia-induced cellular injury. Rapamycin(sirolimus) inhibits protein synthesis through inhibition of the mammalian target ofrapamycin (mTOR). This property of rapamycin has led to its current clinical applications in drug-eluting stents and in immunosuppresive treatment to organ transplant patients. The mechanism …