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Development And In Vivo Evaluation Of A Pura Mutant Strain Of The Channel Catfish Pathogen Edwardsiella Ictaluri., Mark Lee Lawrence
Development And In Vivo Evaluation Of A Pura Mutant Strain Of The Channel Catfish Pathogen Edwardsiella Ictaluri., Mark Lee Lawrence
LSU Historical Dissertations and Theses
Edwardsiella ictaluri is the causative agent of enteric septicemia of catfish (ESC), which is the most serious disease affecting the channel catfish industry. Killed vaccines have been inconsistent in providing protection against ESC, and no live attenuated vaccines have been described. The purpose of this study was to develop a nutritionally attenuated mutant strain of E. ictaluri and evaluate its virulence and vaccine efficacy. An E. ictaluri genomic library was constructed, and a clone containing the purA gene was isolated and sequenced. The E. ictaluri purA gene was found to have several features in common with the E. coli purA …
Dna Wrapping And The Nature Of Interaction Between E. Coli Rna Polymerase And Promoter Dna., Parisa Jazbi
Dna Wrapping And The Nature Of Interaction Between E. Coli Rna Polymerase And Promoter Dna., Parisa Jazbi
LSU Historical Dissertations and Theses
Regulation of transcription most often occurs at the stage of initiation. RNA polymerase binding to the transcription start site, the promoter, is influenced by many nucleotide sequence elements. The predominant recognition sequences are those bound by the $\sigma$ subunit of RNA polymerase located at $-$10 and $-$35 relative to initiation site of most promoters. Another element involved in this regulation is intrinsic DNA curvature. In this study, the contribution of intrinsically curved DNA immediately upstream of the promoter to the interaction between Escherichia coli RNA polymerase and this DNA was examined. DNase I footprinting analysis confirmed that RNA polymerase wraps …
Characterization Of Bean Eukaryotic Translation Initiation Factor 5: Cloning, Sequencing, And Structure-Function Studies., Zelpha Elizabeth Floyd
Characterization Of Bean Eukaryotic Translation Initiation Factor 5: Cloning, Sequencing, And Structure-Function Studies., Zelpha Elizabeth Floyd
LSU Historical Dissertations and Theses
Translation initiation is emerging as one of the important mechanisms involved in regulation of gene expression and our understanding of this process in eukaryotes has greatly increased over the past twenty years. Research in translation initiation in plants has lagged behind efforts in the mammalian system and a number of the initiation factors have not been characterized. Eukaryotic initiation factor 5 (eIF5), the protein mediating hydrolysis of the eIF2-bound GTP and the subsequent formation of the 80S ribosome, is among the least characterized initiation factor in plants. This dissertation describes the cloning of the cDNA encoding eIF5 from a bean …
Identification Of The Physiological Role Of Carbonic Anhydrase Using The Antisense Technique, And, Investigation Of Its Transcriptional Regulation In Arabidopsis Thaliana (L.) Heynh., Hee Jin Kim
LSU Historical Dissertations and Theses
Carbonic anhydrase (CA, EC 4.2.1.1), catalyzes the interconversion of CO$\sb2$ and $\rm HCO\sb3\sp-$ in many organisms. The majority of CA activity in C3 leaves is in the chloroplast and expression of the chloroplast CA is regulated by light. To investigate the physiological role of the chloroplast CA, its expression was suppressed by insertion of a CA transgene in the antisense orientation in Arabidopsis thaliana (L.) Heynhold. Activity assays and immunoblots show that in five independent lines of antisense transgenic plants CA activity was strongly suppressed. All five independent lines died or grew poorly in the absence of sucrose whereas wild …
The Radioresistance Of Deinococcus Radiodurans., Valerie Mattimore
The Radioresistance Of Deinococcus Radiodurans., Valerie Mattimore
LSU Historical Dissertations and Theses
Deinococcus radiodurans is a remarkably ionizing radiation resistant bacterium. As a vegetative cell it is capable of withstanding 5,000 Gy ionizing radiation without evidence of death or mutation. D. radiodurans survives irradiation by repairing DNA damage via an extremely efficient DNA repair system. However, this DNA repair system has not been completely defined. In addition, the selective process allowing the evolution of high level radioresistance has remained a question. This dissertation bolsters the understanding of the DNA repair system of D. radiodurans by characterizing a collection of ionizing radiation sensitive (IRS) mutants. The level of radioresistance is quantified and transformation …