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The Potential For Dickeya Dianthicola To Be Vectored By Two Common Insect Pests Of Potatoes, Jonas K. Insinga

Electronic Theses and Dissertations

Dickeya dianthicola (Samson) causing blackleg and soft rot was first detected in potatoes grown in Maine in 2014. Previous work has suggested that insects, particularly aphids, may be able to vector bacteria in this genus between plants, but no conclusive work has been done to confirm this theory. In order to determine whether insect-mediated transmission is likely to occur in potato fields, two model potato pests common in Maine were used: the Colorado potato beetle (Leptinotarsa decimlineata Say) and the green peach aphids (Myzus persicae Sulzer). Olfactometry and recruitment experiments evaluated if either insect discriminates between infected and …

Landscape Scale: Inter- And Intraspecific Variation In Plant Interactions Along A Stress Gradient In The Sheep Range Of Nevada, Jordan Dowell

UNLV Theses, Dissertations, Professional Papers, and Capstones

Impending threats to shrubland ecosystems, posed by climate change, necessitate niche modeling efforts to project vegetation range shifts. However, efforts often remain unguided by individual-scale interspecific plant interactions. The stress gradient hypothesis posits that facilitation should increase in areas of high abiotic stress, only if the individuals are able to ameliorate the surrounding area via functional traits. The Sheep Range of Nevada was used to assess the role of functional traits as predictors of plant association. Larrea tridentata, Coleogyne ramosissima, and Artemisia nova were selected as shrubs with variable life history strategies and ranges in order to identify general patterns …

Phylogenetic And Genomic Characterization Of The Host-Pathogen Arms Race Between Bacterial Pathogens And Gossypium Hirsutum, Anne Zimmerman Phillips

Arts & Sciences Electronic Theses and Dissertations

Hosts and pathogens are eternally intertwined in an evolutionary arms race. When a pathogen causes a disease outbreak, scientists must identify resistance strategies that can durably tilt the arms race in favor of the host. This requires a deep understanding of both the genetic and environmental contexts in which the outbreak occurs. In this thesis I investigate the bacterial pathogens Xanthomonas citri pv. malvacearum (Xcm) and Pseudomonas syringae that caused disease outbreaks on Gossypium hirsutum from 2011-2017. I use pathogen genomics and host transcriptomics to develop hypotheses for how these pathogens emerged and how they cause disease. Phylogenetics and virulence …

Do Elevation And Aspect Predict Functional Trait Values In Alpine Plant Populations?, Karen Analiesa Bach

Biological Sciences

Alpine regions are among the hardest hit by global climate change, experiencing rising temperatures at a faster rate than lowland areas. Such fast rates of warming are predicted to cause rapid changes in alpine plant communities, including shifts in plant functional traits along elevation gradients. To investigate these effects within species, we examined the relationship between elevation, aspect, and functional traits of three common alpine plants in California; the shrub Holodiscus discolor var. microphyllus, the forb Calyptridium umbellatum, and the grass Trisetum spicatum. Functional traits examined included plant height, plant width, and Specific Leaf Area (SLA). These …

Understanding How The Effectors Hopd1 And Hopg1 From The Bacterial Pathogen Pseudomonas Syringae Pv. Tomato Dc3000 Target The Arabidopsis Thaliana Protein Atnhr2b To Cause Disease In Plants, Catalina Maria Rodriguez Puerto

Graduate Theses and Dissertations

The pathogenicity of Pseudomonas syringae is associated with the type III secretion system (T3SS), a complex of proteins assembled in the inner and outer bacterial membranes that traverses the plant cell wall to deliver bacterial proteins into the cytoplasm of plant cells. The effector proteins translocated into the plant cells are called Hops (Hypersensitive response and pathogenicity outer proteins). Bacterial effectors target plant immune proteins to suppress defense responses and enhance bacterial parasitism. The Arabidopsis thaliana nonhost resistance 2B (AtNHR2B), a recently identified immune protein, is degraded after inoculation with the adapted pathogen of Arabidopsis, P. syringae pv tomato DC3000 …

The Effects Of Seed-Applied Fluopyram On Root Penetration And Development Of Meloidogyne Incognita On Cotton And Soybean, Tracy Hawk

Graduate Theses and Dissertations

Plant-parasitic nematodes are major pests of cotton and soybean in Arkansas, and across the Southern United States. These nematodes cause more than $3 billion worth of crop losses each year, in part due to lack of available control tactics, such as nematicides. Fluopyram has recently been registered as a seed-treatment nematicide in agronomic crops. The toxicity of fluopyram against Meloidogyne incognita infection has been reported, however, information on root protection provided by fluopyram against Meloidogyne incognita is lacking. The first objective of this research was to evaluate the effect seed-applied fluopyram had on nematode development, root galling, and reproduction on …

Use Of Aerial Imagery And Novel Experimental Design To Determine The Distribution Of Foliar Diseases On Soybean And Improve Efficiency Of Product Testing, Mckayla Patterson

Graduate Theses and Dissertations

Soybeans are grown on approximately 1.3 million ha in Arkansas generating an estimated $1.7 billion annually. Foliar diseases on soybean can result in economic losses. Growers spend significant time and money on disease scouting via crop consultants and often (subsequent) fungicide applications. Fungicide trials are often arranged in small plots designs. In these scenarios, spatial variability of foliar disease is minimized. While it is advantageous to minimize variance outside of treatment differences, another limitation with many small plot trials is ample disease pressure or having only lower severity. Within a commercial production field, soil types and disease severities vary. Logically, …

Quantifying The Variability In Heavy Metal Concentrations In Produce Grown In Metals-Rich Soil, Harris Lowell Byers

Theses and Dissertations

Childhood Pb exposure is associated with a multitude of poor health outcomes. In food-insecure areas, growing fresh produce in residential backyard gardens is one option for parents; however, commonly grown crops are known to accumulate Pb in consumable tissues when grown in metals-rich soils. A variety of produce representing a continuum of consumable tissues were grown in soils collected from two residential vegetable gardens, a former metal foundry, and commercial topsoil purchased from a local hardware store. The concentrations of heavy metals in crop tissues were measured with custom wavelength dispersive X-ray Fluorescence (WD-XRF) spectroscopy and portable energy dispersive X-ray …

Determining Water Requirements In Turfgrass Systems, Tyler Quinn Carr

Graduate Theses and Dissertations

Turfgrass lawns are commonly utilized for home and commercial applications because of the aesthetic, environmental, and recreational benefits grasses provide, but many people perceive turfgrass as solely an aesthetic benefit that requires significant water inputs. It is important to identify and use turfgrass cultivars that have known drought tolerance, and also fine-tune and throughout development. A series of field experiments were conducted to determine the water requirements of turfgrass lawn systems with the following objectives: 1) investigate differences in chronic drought resistance and irrigation recommendations among cultivars of Kentucky bluegrass (Poa pratensis L.) and tall fescue (Festuca arundinacea Schreb.) 2) …

Informing Field Management Decisions To Enhance Alfalfa Seed Production Using Remote Sensing, Thomas V. Van Der Weide

Boise State University Theses and Dissertations

The development rate of alfalfa seed crop depends on both environmental conditions and management decisions. Crop management decisions, such as determining when to release pollinators to optimize pollination, can be informed by the identification of plant development stages from remote sensing data. I first identify what electromagnetic wavelengths are sensitive to alfalfa plant development stages using hyperspectral data. A Random Forest regression is used to determine the best Vegetation Index (VI) to monitor how much of the plant is covered in flower. The results indicate that Blue, Green, and Near-Infrared are the important electromagnetic wavelengths for the VI. Imagery collected …

Observable Persistent Effects Of Habitat Management Efforts In The Ozark Highlands After 10 Years, Maxwell Carnes-Mason

Graduate Theses and Dissertations

I investigated the lasting impacts of a management plan designed to improve oak regeneration and benefit wildlife in the Ozark Highlands in Madison, Co., AR. To assess the efficacy of the management plan, I used variables relevant to the success and establishment of oak trees. Controlled burns and selective logging were used to thin the canopy, increase ground level productivity, and increase the abundance of small mammals. I used measurements of overstory and understory densities, light availability, and the density of mice in the genus Peromyscus across time to look at the lasting impacts of management. Different treatment plots were …

The Influence Of Adjacent Weed Populations On Thrips And Iysv In Onion, S. Andrew Swain

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Onion thrips (Thrips tabaci) and Iris Yellow Spot Virus (IYSV) constitute a pest complex of global concern for allium growers. Yield losses due to onion thrips alone can be heavy, and in tandem with IYSV, these losses can be complete.

This study was one phase of a multi-phase research project intent on exploring the potential drivers of thrips and virus infestation in onion. Preliminary observations in participating growers’ fields in northern Utah pointed towards a potential link between weedy field borders and thrips/IYSV infestation in onion. Field work was carried out at the Kaysville experiment station examining the …

Herbage Characteristics Affecting Intake By Dairy Heifers Grazing Grass-Monoculture And Grass-Birdsfoot Trefoil Pastures, Marcus F. Rose

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Pasture-based dairies have become more prevalent in recent years due to a higher proportion of organic milk demand and production. Organic certification requires that animals must graze at least 120 days in each growing season. However, dry matter intake is often limited when dairy animals receive most of their herbage from pasture, resulting in lower animal performance and milk production. The purpose of this study was to analyze the complimentary effect of high energy grasses with birdsfoot trefoil (BFT) tannins to improve intake of dairy heifers. Jersey heifers were rotationally grazed for 105 days in 2017 and 2018 on eight …

Methods To Evaluate Ruminant Animal Production Responses, Jose Manuel Diaz Gomez

Graduate Theses and Dissertations

In experiment 1, 80 steers (197.0 kg initial body weight; BW for fall, 116.9 kg for spring), were stocked at 2.45 and 4.1 calves/ha in fall and spring, respectively in 16 tall fescue pastures [fall ergovaline (EV) = 1,475 ppb and spring EV = 1,173 ppb] under 2 treatments, mineral (MIN) (n = 8) and cumulative management (CM) (n = 8). Forage allowance did not differ (P = 0.76) between CM and MIN during fall but differed during spring (P ≤ 0.05, 2.55 vs. 3.22 kg DM/kg BW, for MIN and CM, respectively). For fall, average daily gain (ADG) resulted …

Evaluation Of Topramezone For Use In Rice (Oryza Sativa L.) Production, Matthew Moore

Graduate Theses and Dissertations

The rapid development of resistant weeds, particularly to postemergence-applied herbicides, is a growing concern to rice producers worldwide. Barnyardgrass, one of the most problematic weeds in rice cropping systems, alone has been found to be resistant to four herbicide sites of action (SOA) in certain populations, leaving growers no options for control. This unsettling fact has led to research on other SOAs that have not previously been used in US rice. One SOA that has garnered interest is the 4-hydroxyphenylpyruvate dioxygenase (HPPD)- inhibiting herbicides. The only HPPD-inhibitor that will soon be labeled for use in US rice is benzobicyclon. The …

Mitigating Herbicide Impacts To Soybean, Grant Lawson Priess

Graduate Theses and Dissertations

The rapidity in evolution of herbicide-resistant weeds and the resulting cost to U.S. farmers demonstrate the need to responsibly steward the limited number of herbicides available in agricultural systems. To reduce weed emergence and likewise added selection pressures placed on herbicides, early-season crop canopy formation has been promoted. However, impacts to soybean following a potentially injurious herbicide application have not been thoroughly evaluated. Therefore, field experiments were conducted to determine whether: 1) soybean injury from metribuzin or flumioxazin delayed canopy formation or changed the incidence of pathogen colonization; 2) residual herbicides applied preplant reduced the potential for soybean injury and …

Effects Of Water Table Depth And Edaphic Characteristics On Plant Diversity In A Southern Mississippi Pitcher Plant Bog, Patrick Kirby

Master's Theses

This study examined the effects that water table depth and soil characteristics have on plant species richness and species composition within pitcher plant bogs across seasons. Eight piezometers were installed at random distances to monitor long-term water table depth and pressure fluctuations along a ~710-meter line transect traversing upland and bog habitats. Vegetation sampling quadrats (n=128) were set up near each piezometer. Cover data and water table depths were collected in spring and late summer. Soil samples collected from each treatment group were used to obtain soil texture and nutrient data. The summer collection period yielded a total gdiversity of …

Analyzing Multigene Stacking And Genome Editing Strategies In Rice, Bhuvan Pathak

Graduate Theses and Dissertations

Crop improvement through biotechnology is an integrated effort, incorporating multiple approaches like integration of genes, editing of native genes, and removal of selection marker genes. Before streamlining the protocols, the efficiency and feasibility of the individual approach and their components must be tested. This study evaluated following approaches: 1) stacking an array of genes into a single locus by site-specific integration via Cre-lox recombination in rice, 2) determining the efficiency of I-SceI and the CCR5-ZFN in the targeted excisions of gene fragments in rice and Arabidopsis, and 3) determining the efficiency of CRISPR/Cas9 in generating targeted mutations for genome editing …

Qtl Mapping Of Panicle Architecture And Yield-Related Traits Between Two U.S. Rice Cultivars 'Lagrue' And 'Lemont', Adam D. Rice

Graduate Theses and Dissertations

Grain yield in rice consists of multiple yield components such as number of panicles/plant, number of tillers/plant, and number of seeds/panicle. Panicle architecture is an important yield trait yield as the number of seeds on a panicle greatly contributes to the overall panicle yield. A QTL analysis and mapping study on panicle architecture and yield-related traits was performed to identify major QTL and candidate genes associated with the traits. The first objective of the project was to do a yield study evaluating 15 agronomic traits between each of the four U.S. rice cultivars, ‘LaGrue’, ‘Lemont’, ‘Bengal’, and ‘Mars’, to determine …

Comparison Of Morphological Traits In Cowpea Pod Length, Seed Number Per Pod, Seed Weight, Seed Color And Seed Density In Usda Germplasm Accessions And Arkansas Breeding Lines, Sora Imamura

Crop, Soil and Environmental Sciences Undergraduate Honors Theses

The objective of the study was to compare and correlate the Arkansas cowpea breeding lines to the USDA cowpea germplasm accessions on the following morphological traits: seed color, pod length, number of seeds per pod, 100 seed weight, and seed density. The seed density is defined as the weight of seed in gram per centimeter of pod length. Three hundred seventeen cowpea genotypes were used in this experiment. Out of the 317, 285 germplasm accessions were from the USDA GRIN and 32 lines were from the Arkansas breeding program.

T-test between Arkansas breeding lines and USDA germplasm accessions were done …

A Seascape Genetics Approach To Studying Genetic Differentiation In The Bull Kelp Nereocystis Luetkeana, Lily G. Gierke

Theses and Dissertations

The brown alga Nereocystis luetkeana is a foundation species found from Alaska to California. In the Salish Sea, N. luetkeana is declining, but little is known about its population structure. We explored N. luetkeana 1) allelic dissimilarity and richness using seven microsatellite markers, and 2) tested models of gene flow in the Salish Sea using a hydrodynamic transport model. Our results suggest that the N. luetkeana distribution is comprised of four genetic co-ancestry groups that are geographically coherent, apart from the separation of the Strait of Georgia/Puget Sound by the Strait of Juan de Fuca. Our model supported that environmental …

Genetic Resistance To The Downy Mildew Pathogen And Mapping The Rpf Resistance Loci In Spinach, Gehendra Bhattarai

Graduate Theses and Dissertations

Spinach (Spinacia oleracea) is an important cool-season leafy vegetable crop in the United States (US). Downy mildew, caused by the obligate oomycete Peronospora effusa, is the most economically important disease of spinach. A total of 17 races of P. effusa have been reported on spinach, and many of these races (>10) have emerged in the last three decades. The new races of the pathogen are continually overcoming the genetic resistances used in the newly released cultivars.

A detached leaf inoculation assay was evaluated, standardized, and validated as a new method to differentiate resistant and susceptible spinach genotypes. Disease response …

An Evaluation Of Hybrid Traits, Yield, And Major Qtl Effect On Heterosis In Hybrid Soft Red Winter Wheat, Zachary James Winn

Graduate Theses and Dissertations

Wheat (Triticum aestivum L.) is a self-pollinating species that is most often bred as a recombinant inbred line. When two inbred individuals are crossed, they produce progeny which experience heterosis; heterosis is the increased robustness experienced due to the reintroduction of heterozygosity. Hybrid wheat may be produced through the use of chemical hybridization agents, yet the structure of wheat flowers decreases the efficiency of outcrossing. The objectives of this study were to: assess the amount of heterosis experienced by a population of hybrid wheat and observe the effect of major gene loci on yield and yield components, phenotype anthers extruded …

Cover Crops As An Integrated Approach For Pest Suppression And Pollinator Promotion In Arkansas Watermelon Production Systems, Paige Laurel Hickman

Graduate Theses and Dissertations

Arkansas watermelon growers face a variety of insect pest and disease problems that have the potential to threaten yield. Integrated pest management tactics can provide control over these threats and are intended to cut down on pesticide use and its associated negative impacts like pesticide resistance, non-target effects, and runoff. Cover crops provide an array of benefits and can be useful in integrated pest management. Evidence has shown that certain cover crops can increase beneficial insects and suppress disease in the following cash crop. They can also provide important resources to pollinators. In order to build a better understanding of …

Nematode Populations As Affected By Residue And Water Management In A Long-Term Wheat-Soybean Double Crop In Eastern Arkansas, Lucia Emperatriz Escalante Ortiz

Graduate Theses and Dissertations

Soybeans (Glycine max) are one of the major row crops in the United States, particularly in Arkansas. Soybean cyst nematode (SCN, Heterodera glycines) and southern root-knot nematode (RKN, Meloidogyne incognita) are two of the most damaging pests that cause major economic losses in soybeans. Little is known concerning the effects of common and alternative agronomic practices on nematodes in fields with nematode population densities below threshold levels. Therefore, the objective of this study was to evaluate the effects of the combination of tillage (conventional tillage and no-tillage), irrigation (irrigated and non-irrigated), wheat (Triticum aestivum) residue burning (burned and no burned), …

Use Of Harvest Weed Seed Control Strategies In Arkansas Soybean, Jeremy Kyle Green

Graduate Theses and Dissertations

Today, most growers use chemical weed management programs; however, a sole reliance on herbicides will place more resistance selection pressure on the weeds to which the herbicide is being applied. As herbicide resistance continues to grow and rob growers of yield, alternative weed control options are being sought to create complex integrated weed management programs to prolong the use of effective herbicides. Harvest weed seed control (HWSC) is a non-chemical practice that has been widely adopted in Australia due to herbicide resistance problems. In most cases, herbicide-resistant weeds that survive applications of herbicides produce viable seed that pass through the …

Phenotypic Study Of Anthracnose Resistance In Black Walnut And Building A Mapping Population, Sadie D. Land

MSU Graduate Theses

Black walnut anthracnose, caused by Gnomonia leptostyla, is the most widespread and destructive disease affecting black walnut trees (Juglans nigra). Breeding cultivars for a higher resistance to anthracnose is a natural and efficient strategy for improving the health and production quality of black walnut trees. The two goals of this study were to reveal that the ‘Sparrow’ cultivar of black walnut contains a significantly higher resistance to anthracnose than the ‘Football’ cultivar when separated from environmental factors, and to expand the ‘Football’ × ‘Sparrow’ F1 mapping population to evaluate how the trait of resistance is inherited in the progeny. A …

Investigating Dicamba Tolerance In Grapevine Cultivars Through Drift Simulation Assays, Bryce David Bentley

MSU Graduate Theses

Dicamba is a synthetic auxin herbicide which acts systematically and selectively controls broadleaf plants and has become increasingly popular in the past several years for weed control in commercial agriculture following widespread adoption of dicamba-resistant, transgenic soybean and cotton. Dicamba may be better acknowledged by growers of specialty crops, like grapes, for its capacity to drift miles away from the site of application and deposit on fields, remaining potent enough to cause crop damage and yield loss to vulnerable plants. Grapes are among the most susceptible agronomic crops to dicamba drift, showing visible injury at rates less than 1% of …

Evaluation Of Pheromone Mating Disruption For California Red Scale Control In Commercial California Citrus, Joel Timothy Leonard

Master's Theses

California red scale (CRS), Aonidella aurantii, is an increasingly injurious insect pest for the California citrus industry due to insecticide resistance, changing weather patterns, and shifting trade regulations. The presence of the insect on fruit, damages the rind of the fruit and high populations can cause dieback of branches as well as lower yields. Pheromone mating disruption of CRS has the potential to alleviate population control concerns and reduce insecticide use. The efficacy of the pheromone mating disruption technique for pest management of CRS was determined using the products CheckMate® CRS and Semios CRS Plus. CheckMate® CRS was evaluated …

Vines In The Neotropics: Phylogenomics, Biogeography And Systematics In Passion Flowers (Passiflora Subgenus Decaloba Section Decaloba), Serena Acha

Dissertations

With 600 species, Passiflora is a large, morphologically complex and broadly distributed genus in Passifloraceae that represents a major challenge to scientists interested in understanding the evolutionary history of tropical vines. Passiflora has been divided into subgenera, super sections and sections. One of the most enigmatic and species-rich (~120 spp.) groups in Passiflora is section Decaloba, which occurs in the Neotropics and is particularly diverse in Andean montane forests. In this study, we used phylogenomic and population genomic approaches to investigate the evolutionary history, biologeography, species boundaries, and taxonomy of Passiflora section Decaloba. We sampled herbarium specimens, extracted …