Digital Commons Network^™

Recent Advances In Basic Neurosciences And Brain Disease: From Synapses To Behavior, Guo-Qiang Bi, Vadim Bolshakov, Guojun Bu, Catherine M. Cahill, Zhou-Feng Chen, Graham L. Collingridge, Robin L. Cooper, Jens R. Coorssen, Alaa El-Husseini, Vasco Galhardo, Wen-Biao Gan, Jianguo Gu, Kazuhide Inoue, John Isaac, Koichi Iwata, Zhengping Jia, Bong-Kiun Kaang, Mikito Kawamata, Satoshi Kida, Eric Klann, Tatsuro Kohno, Min Li, Xiao-Jiang Li, John F. Macdonald, Karim Nader, Peter V. Nguyen, Uhtaek Oh, Ke Ren, John C. Roder, Michael W. Salter, Weihong Song, Shuzo Sugita, Shao-Jun Tang, Yuanxiang Tao, Yu Tian Wang, Newton Woo, Melanie A. Woodin, Zhen Yan, Megumu Yoshimura, Ming Xu, Zao C. Xu, Xia Zhang, Mei Zhen, Min Zhuo

Biology Faculty Publications

Understanding basic neuronal mechanisms hold the hope for future treatment of brain disease. The 1st international conference on synapse, memory, drug addiction and pain was held in beautiful downtown Toronto, Canada on August 21-23, 2006. Unlike other traditional conferences, this new meeting focused on three major aims: (1) to promote new and cutting edge research in neuroscience; (2) to encourage international information exchange and scientific collaborations; and (3) to provide a platform for active scientists to discuss new findings. Up to 64 investigators presented their recent discoveries, from basic synaptic mechanisms to genes related to human brain disease. This meeting …

Recombinant Watermelon (Citrullus Lanatus) Hydroperoxide Lyase And Uses Thereof, David Hildebrand, Hirotada Fukushige

Plant and Soil Sciences Faculty Patents

Recombinant watermelon (Citrullus lanatus) hydroperoxide lyase protein, DNA sequences encoding the protein, vectors containing the DNA sequences and hosts containing the vectors are provided, together with methods for recombinantly producing watermelon hydroperoxide lyase, DNA sequences, vectors and hosts.

Modified Soy Products And Methods For Reducing Odor And Improving Flavor Of Soy Products, William L. Boatright

Animal and Food Sciences Faculty Patents

A method for reducing odor in soy products and compositions containing soy products by adding or admixing a compound having one or more disulfide bonds to the soy product or composition. The disulfide compound includes a peptide containing at least cystine residue, a peptide or polypeptide containing at least one disulfide bond, L-cystine, D-cystine, DL-cystine and any combination thereof.

Evidence That Talin Alternative Splice Variants From Ciona Intestinalis Have Different Roles In Cell Adhesion, Richard H. Singiser, Richard O. Mccann

Molecular and Cellular Biochemistry Faculty Publications

BACKGROUND: Talins are large, modular cytoskeletal proteins found in animals and amoebozoans such as Dictyostelium discoideum. Since the identification of a second talin gene in vertebrates, it has become increasingly clear that vertebrate Talin1 and Talin2 have non-redundant roles as essential links between integrins and the actin cytoskeleton in distinct plasma membrane-associated adhesion complexes. The conserved C-terminal I/LWEQ module is important for talin function. This structural element mediates the interaction of talins with F-actin. The I/LWEQ module also targets mammalian Talin1 to focal adhesion complexes, which are dynamic multicomponent assemblies required for cell adhesion and cell motility. Although Talin1 is …

Forage News [2006-12], Department Of Plant And Soil Sciences, University Of Kentucky

Forage News

• Grazing Conference
• FEED: Biggest Cost Item
• Heart of America Grazing Conference
• KFGC Presents Annual Awards
• KFGC Installs New Officers
• Fayette County Producer Becomes Forage Spokesperson
• Kentucky Hay – 2006
• Methods to Manage Soil Phosphorus Levels on Kentucky Poultry Farms
• Meat Goat Performance and Carcass Parameters When Finished on Orchardgrass, Red Clover, or Alfalfa Pastures
• The Case for Forage Legumes
• Upcoming Events

The Pulmonary Effects Of Intravenous Adenosine In Asthmatic Subjects, Nausherwan K. Burki, Mahmud Alam, Lu-Yuan Lee

Physiology Faculty Publications

BACKGROUND: We have shown that intravenous adenosine in normal subjects does not cause bronchospasm, but causes dyspnea, most likely by an effect on vagal C fibers in the lungs [Burki et al. J Appl Physiol 2005; 98:180-5]. Since airways inflammation and bronchial hyperreactivity are features of asthma, it is possible that intravenous adenosine may be associated with an increased intensity of dyspnea, and may cause bronchospasm, as noted anecdotally in previous reports.

METHODS: We compared the effects of placebo and 10 mg intravenous adenosine, in 6 normal and 6 asthmatic subjects.

RESULTS: Placebo injection had no significant (p > 0.05) effect …

Hydrologic Properties Of Pervious Concrete, Joe D. Luck, Stephen R. Workman, Stephen F. Higgins, Mark S. Coyne

Plant and Soil Sciences Faculty Publications

Pervious concrete is concrete made by eliminating most or all of the fine aggregate (sand) in the concrete mix, which allows interconnected void spaces to be formed in the hardened product. These interconnected void spaces allow the concrete to transmit water at relatively high rates. The main objective of this project was to conduct research on the potential application of pervious concrete in agricultural settings, specifically for use in animal feed lots, manure storage pads, animal manure and bedding compost facilities, or floor systems in animal buildings. Laboratory tests were conducted on replicated samples of pervious concrete formed from two …

Forage News [2006-11], Department Of Plant And Soil Sciences, University Of Kentucky

Forage News

• Kentucky Grazing Conference in Lexington, November 21
• Cash Receipts Highlights – 2005
• Loss of a Legend
• Hay Is in the Barn…
• New Executive Secretary for AFGC
• Alfalfa’s Makeover
• Effect of Sericea Lespedeza Hay on Gastrointestinal Nematode Infection in Goats
• Upcoming Events

The Role Of Mapks In B Cell Receptor-Induced Down-Regulation Of Egr-1 In Immature B Lymphoma Cells, Jiyuan Ke, Murali Gururajan, Anupam Kumar, Alan Simmons, Lilia Turcios, Ralph Lakshman Chelvarajan, David M. Cohen, David L. Wiest, John G. Monroe, Subbarao Bondada

Microbiology, Immunology, and Molecular Genetics Faculty Publications

Cross-linking of the B cell receptor (BCR) on the immature B lymphoma cell line BKS-2 induces growth inhibition and apoptosis accompanied by rapid down-regulation of the immediate-early gene egr-1. In these lymphoma cells, egr-1 is expressed constitutively and has a prosurvival role, as Egr-1-specific antisense oligonucleotides or expression of a dominant-negative inhibitor of Egr-1 also prevented the growth of BKS-2 cells. Moreover, enhancement of Egr-1 protein with phorbol 12-myristate 13-acetate or an egr-1 expression vector rescued BKS-2 cells from BCR signal-induced growth inhibition. Nuclear run-on and mRNA stability assays indicated that BCR-derived signals act at the transcriptional level to …

The Generation And Function Of Soluble Apoe Receptors In The Cns, G. William Rebeck, Mary Jo Ladu, Steven Estus, Guojun Bu, Edwin J. Weeber

Physiology Faculty Publications

More than a decade has passed since apolipoprotein E4 (APOE-epsilon4) was identified as a primary risk factor for Alzheimer 's disease (AD), yet researchers are even now struggling to understand how the apolipoprotein system integrates into the puzzle of AD etiology. The specific pathological actions of apoE4, methods of modulating apolipoprotein E4-associated risk, and possible roles of apoE in normal synaptic function are still being debated. These critical questions will never be fully answered without a complete understanding of the life cycle of the apolipoprotein receptors that mediate the uptake, signaling, and degradation of apoE. The present review will focus …

Prevalence And Association Of The Laboulbenialean Fungus Hesperomyces Virescens (Laboulbeniales: Laboulbeniaceae) On Coccinellid Hosts (Coleoptera: Coccinellidae) In Kentucky, Usa, James D. Harwood, Carlo Ricci, Roberto Romani, Kevin M. Pitz, Alex Weir, John J. Obrycki

Entomology Faculty Publications

The laboulbenialean fungi occur throughout the world and are closely associated with a range of arthropods, including many coleopteran hosts. Throughout the summer of 2004, coccinellids were collected from a Bluegrass savanna woodland ecosystem, dominated by blue ash Fraxinus quadrangulata and Chinkapin Oak Quercus muehlenbergii, and the adults were examined for the presence of Hesperomyces virescens using binocular and scanning electron microscopy. Over 80% of adult Harmonia axyridis, a species previously reported as having a persistent association with the fungus, were infected. No significant differences were observed in incidence on male and female hosts, however, the distribution of …

Forage News [2006-10], Department Of Plant And Soil Sciences, University Of Kentucky

Forage News

• KFGC Field Day Huge Success in Spite of 4” of Rain
• Roundup Ready Grazing Tolerant Alfalfa: Application for Kentucky
• 26 Roundup Ready Alfalfa Varieties
• Hay Export Markets May Benefit All Growers
• Prevent Machinery Fires
• Ethanol Growth Challenges Hay Markets
• Hay Production Up
• Upcoming Events

Agricultural Situation And Outlook Fall 2006, James Mansfield, Timothy A. Woods

Agricultural Situation and Outlook

No abstract provided.

Biologic Cycling Of Silica Across A Grassland Bioclimosequence, S. W. Blecker, Rebecca L. Mcculley, O. A. Chadwick, E. F. Kelly

Plant and Soil Sciences Faculty Publications

The dynamics of biologic Si cycling in grassland ecosystems are largely unknown and likely to impact mineral weathering rates regionally and diatom productivity globally; key regulatory processes in the global Si cycle are closely tied to the global carbon cycle. Across a bioclimatic sequence spanning major grassland ecosystems in the Great Plains, soil biogenic silica depth distributions are similar to that of soil organic carbon; however, unlike soil organic carbon, quantities of soil biogenic silica decrease with increasing precipitation, despite an increase in annual biogenic inputs through litterfall across the same gradient. Though comprising only 1–3% of the total Si …

Forage News [2006-09], Department Of Plant And Soil Sciences, University Of Kentucky

Forage News

• Forage Field Day
• Stockpile Production: Does It Pay to Fertilize in Fall Given the High Cost of Nitrogen Fertilizer?
• Can You Successfully “Thicken-Up” Old Alfalfa Stands?
• Fall Seeding Grasses
• What a Difference a Year Makes…
• Monitor Wet Hay to Prevent Fire
• Forage Spokesman Nominations
• Rake Design Doesn’t Impact Hay Quality
• KFGC Awards Nominations
• Upcoming Events

Gene Order Data From A Model Amphibian (Ambystoma): New Perspectives On Vertebrate Genome Structure And Evolution, Jeramiah J. Smith, S. Randal Voss

Biology Faculty Publications

BACKGROUND: Because amphibians arise from a branch of the vertebrate evolutionary tree that is juxtaposed between fishes and amniotes, they provide important comparative perspective for reconstructing character changes that have occurred during vertebrate evolution. Here, we report the first comparative study of vertebrate genome structure that includes a representative amphibian. We used 491 transcribed sequences from a salamander (Ambystoma) genetic map and whole genome assemblies for human, mouse, rat, dog, chicken, zebrafish, and the freshwater pufferfish Tetraodon nigroviridis to compare gene orders and rearrangement rates.

RESULTS: Ambystoma has experienced a rate of genome rearrangement that is substantially lower than mammalian …

Forage News [2006-08], Department Of Plant And Soil Sciences, University Of Kentucky

Forage News

• KFGC Awards Nominations
• Forage Spokesman Nominations
• UK Robinson Station to Host College of Ag Field Day
• Kentucky Department of Agriculture Forage Testing Program
• More Hay…..
• Guidelines for Successful Forage Establishment
• Upcoming Events

Development And Testing Of A Low-Cost Condensation Detection System, Michael D. Montross, George A. Duncan, Richard S. Gates

Biosystems and Agricultural Engineering Faculty Publications

A condensation sensing and control system was designed to detect condensation using a commercially available leaf wetness sensor (LWS). The leaf wetness sensor was a variable resistance grid-type that responded to moisture on the surface. A circuit was developed to compare the LWS voltage output to a user specified reference voltage, and operate a relay for possible switching of a humidity control device (for example a fan and/or heater). The condensation detection system operation was validated in an environmental chamber in the laboratory using a heat exchanger and water bath. Condensate was immediately detected when the plate was cooled below …

Forage News [2006-07], Department Of Plant And Soil Sciences, University Of Kentucky

Forage News

• Determining Forage Moisture Content Using a Microwave Oven
• KFGC Field Day
• Kentucky: Did You Know
• KFGC Awards Program
• Forage Spokesman Nominations
• Hay Storage Demonstration Farm Project Evaluating Impact of Indoor Storage
• Agrotain Reduces Ammonia Loss from Surface-Applied Urea
• Grazing Corn
• Upcoming Events

Dissociation Of Automatic And Strategic Lexical-Semantics: Functional Magnetic Resonance Imaging Evidence For Differing Roles Of Multiple Frontotemporal Regions, Brian T. Gold, David A. Balota, Sara J. Jones, David K. Powell, Charles D. Smith, Anders H. Andersen

Neuroscience Faculty Publications

Behavioral research has demonstrated three major components of the lexical-semantic processing system: automatic activation of semantic representations, strategic retrieval of semantic representations, and inhibition of competitors. However, these component processes are inherently conflated in explicit lexical-semantic decision tasks typically used in functional magnetic resonance imaging (fMRI) research. Here, we combine the logic of behavioral priming studies and the neurophysiological phenomenon of fMRI priming to dissociate the neural bases of automatic and strategic lexical-semantic processes across a series of three studies. A single lexical decision task was used in all studies, with stimulus onset asynchrony or linguistic relationship between prime and …

Forage News [2006-06], Department Of Plant And Soil Sciences, University Of Kentucky

Forage News

• UK Animal Research Center Field Day
• Effect of Storage Method on Round Bale Hay Loss
• Kentucky Milk Production
• Kentucky Welcomes Dr. Rebecca McCulley
• Average Prices Received by Farmers–(Dollars)
• Bioterrorism & Hay
• Reducing Broiler Litter Application Rates Can Still Produce High Yield and Quality in Sorghum Sudangrass
• Measuring Variation in Alfalfa Yield and Stand Using Conventional Remote Sensing Techniques
• Upcoming Events

Methods And Compositions For Expressing Multiple Genes In Plants By Alternate Splicing Of A Polycistronic Message, Indu Bhushan Maiti, Somnath Bhattacharyya

Kentucky Tobacco Research and Development Center Faculty Patents

The present invention provides a method and composition for expression of multiple genes from a polycistronic message in transgenic plants using genetic elements derived from the peanut chlorotic streak caulimovirus promoter-leader sequence and antisense sequence of PC1SV ORF VII. Also provided are compositions and methods for intron-mediated enhanced and regulated expression of genes in transgenic plants.

Forage News [2006-05], Department Of Plant And Soil Sciences, University Of Kentucky

Forage News

• Barren County Continued as Cattle Capital of Kentucky
• Storing Alfalfa as Round Bale Silage: Frequently Asked Questions
• Cowpea Aphid in Kentucky Alfalfa
• Disk vs. Sickle Cutterbar Mowers
• Organic Hay
• UK Beef/Forage Field Day June 15
• Last Chance!!!
• Upcoming Events

Shelling Out For Genomics, Timothy S. Mcclintock, Charles D. Derby

Physiology Faculty Publications

A report on the symposium 'Genomic and Proteomic Approaches to Crustacean Biology' held as part of the Society for Integrative and Comparative Biology 2006 Annual Meeting, Orlando, USA, 4-8 January 2006.

Prenatal Cocaine Exposure Alters Alpha2 Receptor Expression In Adolescent Rats, Rosemarie M. Booze, David R. Wallace, Janelle M. Silvers, Barbara J. Strupp, Diane M. Snow, Charles F. Mactutus

Neuroscience Faculty Publications

BACKGROUND: Prenatal cocaine exposure produces attentional deficits which to persist through early childhood. Given the role of norepinephrine (NE) in attentional processes, we examined the forebrain NE systems from prenatal cocaine exposed rats. Cocaine was administered during pregnancy via the clinically relevant intravenous route of administration. Specifically, we measured alpha2-adrenergic receptor (alpha2-AR) density in adolescent (35-days-old) rats, using [3H]RX821002 (5 nM).

RESULTS: Sex-specific alterations of alpha2-AR were found in the hippocampus and amygdala of the cocaine-exposed animals, as well as an upregulation of alpha2-AR in parietal cortex.

CONCLUSION: These data suggest that prenatal cocaine exposure results in a persistent alteration …

Forage News [2006-04], Department Of Plant And Soil Sciences, University Of Kentucky

Forage News

• 27^th Kentucky Alfalfa Conference Date Set
• 7^th Kentucky Grazing Conference Date Set
• Heart of America Grazing Conference in Illinois
• Proceedings Available On-Line
• KFGC Forage Field Day
• Kentucky Producer Wins 1^st Place at AFGC Hay Show
• UK Robinson Station to Host College of Ag Field Day
• Kentucky Reaps Awards at AFGC Meeting
• WKU Students Play Prominent Role at AFGC Meeting
• Rooting Depth of Alfalfa
• Environmental and Economic Evaluation of Grass and Corn Based Production Systems
• On a Maryland Beef Farm
• The Economics of Organic and Grazing Dairy Farms
• Reminder - Don’t Forget!!!
• Upcoming Events

Cyclooxygenase-2 Mediates Microglial Activation And Secondary Dopaminergic Cell Death In The Mouse Mptp Model Of Parkinson's Disease, Rattanavijit Vijitruth, Mei Liu, Dong-Young Choi, Xuan V. Nguyen, Randy L. Hunter, Guoying Bing

Neuroscience Faculty Publications

BACKGROUND: Accumulating evidence suggests that inflammation plays an important role in the progression of Parkinson's disease (PD). Among many inflammatory factors found in the PD brain, cyclooxygenase (COX), specifically the inducible isoform, COX-2, is believed to be a critical enzyme in the inflammatory response. Induction of COX-2 is also found in an experimental model of PD produced by administration of 1-methy-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP).

METHOD: COX-2-deficient mice or C57BL/6 mice were treated with MPTP to investigate the effects of COX-2 deficiency or by using various doses of valdecoxib, a specific COX-2 inhibitor, which induces inhibition of COX-2 on dopaminergic neuronal toxicity and …

Forage News [2006-03], Department Of Plant And Soil Sciences, University Of Kentucky

Forage News

• 26^th Kentucky Alfalfa Conference
• Alfalfa Award Recipients Honored
• Improved Alfalfa Varieties
• Kentucky Forage & Grassland Council Awards for 2006
• How are Pasture Quality, Soil Quality, and Soil Organic Carbon Content Linked? Lessons from South Central Iowa
• Common Red Clover
• Poultry Litter Rate Study in Tall Fescue
• Stockpiling Publication Revised
• Mailing List Update
• Upcoming Events

[Review Of] Stable Isotopes And Biosphere–Atmosphere Interactions: Processes And Biological Controls, Mark S. Coyne

Plant and Soil Sciences Faculty Publications

No abstract provided.

Cbfbeta Is A Facultative Runx Partner In The Sea Urchin Embryo, Anthony J. Robertson, Carrie Dickey-Sims, Andrew Ransick, Dawn E. Rupp, John J. Mccarthy, James A. Coffman

Physiology Faculty Publications

BACKGROUND: Runx proteins are developmentally important metazoan transcription factors that form a heterodimeric complex with the non-homologous protein Core Binding Factor beta (CBFbeta). CBFbeta allosterically enhances Runx DNA binding but does not bind DNA itself. We report the initial characterization of SpCBFbeta, the heterodimeric partner of SpRunt-1 from the sea urchin Stronylocentrotus purpuratus.

RESULTS: SpCBFbeta is remarkably similar to its mammalian homologues, and like them it enhances the DNA binding of the Runt domain. SpCBFbeta is entirely of zygotic provenance and its expression is similar that of SpRunt-1, accumulating globally at late blastula stage then later localizing to endoderm and …