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2006

Iowa State University

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Synthesis And Characterization Of Hydrated Rubidium Thio-Hydroxosilicogermanates Using Mechanochemical Hydrothermal Synthesis, Carly R. Nelson, Sarah Olson, Steven A. Poling, Steve W. Martin Dec 2006

Synthesis And Characterization Of Hydrated Rubidium Thio-Hydroxosilicogermanates Using Mechanochemical Hydrothermal Synthesis, Carly R. Nelson, Sarah Olson, Steven A. Poling, Steve W. Martin

Materials Science and Engineering Publications

The synthesis, structure, and proton conductivity of the hydrated thio-hydroxosilicogermanates RbzGexSi1-xSz(OH)4-z·yH2O (2 ≤ z ≤ 3; 0 < y < 1.7) are reported. X-ray diffraction indicates that the z = 2 materials are amorphous and show no unreacted starting materials, which is consistent with the mechanochemical hydrothermal evaporation−precipitation synthesis method. Infrared spectroscopy shows a fully reacted system where both silicon and germanium central anions have vibrational modes at 950, 780, 667, and 450 cm-1 associated with asymmetric modes for Si−O, Ge−O, Si−S, and Ge−S chemical bonds, respectively. O−H stretching and bending modes are present at ...


Behavioral Subtyping Is Equivalent To Modular Reasoning For Object-Oriented Programs, Gary T. Leavens, David A. Naumann Dec 2006

Behavioral Subtyping Is Equivalent To Modular Reasoning For Object-Oriented Programs, Gary T. Leavens, David A. Naumann

Computer Science Technical Reports

Behavioral subtyping is an established idea that enables modular reasoning about behavioral properties of object-oriented programs. It requires that syntactic subtypes are behavioral refinements. It validates reasoning about a dynamically-dispatched method call, say E.m(), using the specification associated with the static type of the receiver expression E. For languages with references and mutable objects the idea of behavioral subtyping has not been rigorously formalized as such and the standard informal notion has inadequacies. This paper formalizes behavioral subtyping and introduces a new formalization of modular reasoning, called supertype abstraction. A Java-like sequential language is considered, with classes and interfaces ...


Carbon Monoxide Mass Transfer In A Syngas Mixture, Amir Kapic, Samuel T. Jones, Theodore J. Heindel Dec 2006

Carbon Monoxide Mass Transfer In A Syngas Mixture, Amir Kapic, Samuel T. Jones, Theodore J. Heindel

Mechanical Engineering Publications

A myoglobin assay for measuring concentrations of dissolved carbon monoxide (CO) from an artificial synthesis gas blend (20% CO, 18% CO2, 52% N2, and 10% H2) was utilized to determine volumetric CO−water mass-transfer rates in a 0.211-m-diameter stirred-tank reactor (STR). The data are well correlated using the power density and superficial gas velocity, but this correlation is valid only for this STR size. A gas−liquid scale-up model developed for dissolved oxygen mass-transfer rates using air was used to develop a CO−liquid scale-up model for syngas fermentation. The model is applicable in the STR hydrodynamic range associated ...


New Extension Corn Production Web Site, Lori Abendroth, Roger W. Elmore Dec 2006

New Extension Corn Production Web Site, Lori Abendroth, Roger W. Elmore

Integrated Crop Management News

We've designed a new Web site for Iowa State University Extension corn production that has current and relevant management information for producing corn in Iowa. The Web site provides research-based recommendations and diagnostic tools for producers and agribusinesses. The largest section of the Web site is devoted to "Corn Management," which is broken into categories based on whether the topic addresses overarching production issues, such as cropping systems and rotations, or if the topic can be isolated to a certain time of the growing season (planting, early-season, mid-/late-season, harvest). For example, in the "Planting" category, users can find ...


A Response To Phenomenon In 2006: Multiple Ears Per Node, Roger W. Elmore, Lori Abendroth Dec 2006

A Response To Phenomenon In 2006: Multiple Ears Per Node, Roger W. Elmore, Lori Abendroth

Integrated Crop Management News

Corn hybrids from several companies expressed more than one ear at a single node from Iowa to Indiana in 2006. Multiple ears are not unexpected, but they typically occur at different nodes (as with prolific hybrids), not on the same node. This trait was expressed in different ways in Iowa. In the most extreme cases, up to eight ears occurred at a single node. Some have called these "bouquets." Ears on these plants were usually barren. In one case, a field with bouquet ears yielded 50 bu/acre.


How To Interpret Scn Soil Test Results, Gregory L. Tylka Dec 2006

How To Interpret Scn Soil Test Results, Gregory L. Tylka

Integrated Crop Management News

Soybean yield loss due to the soybean cyst nematode (SCN) occurred throughout much of Iowa in 2006. Damage from this pest was particularly noticeable in areas of the state that were very dry. There seems to be increased interest in testing for and managing SCN in Iowa this fall, and there likely has been more fields sampled for SCN this fall than in recent years. Following are some commonly asked questions and answers that illustrate things to consider when interpreting SCN soil sample results. This information is excerpted from Iowa State University Extension publication IPM 61, Interpreting SCN Soil Sample ...


Crop Performance Test Bulletins Now Available, Keven Arrowsmith Dec 2006

Crop Performance Test Bulletins Now Available, Keven Arrowsmith

Integrated Crop Management News

Results of the 2006 Iowa Crop Performance Tests for alfalfa, barley, corn, oat, soybean, triticale, and winter wheat are now available. For those wanting to download a copy of the bulletins, visit the Iowa Crop Improvement Association (ICIA) Web site or the Iowa State University (ISU) Extension Distribution Center (EDC) online store. Published bulletins may be requested by contacting ICIA at (515) 294-6921 or EDC at (515) 294-5247.


Aphids In The Air: What Is The Risk For 2007?, Wayne Ohnesorg, Matthew E. O'Neal, Marlin E. Rice Dec 2006

Aphids In The Air: What Is The Risk For 2007?, Wayne Ohnesorg, Matthew E. O'Neal, Marlin E. Rice

Integrated Crop Management News

The 2006 growing season has come to a close and with it another year's worth of experience with the soybean aphid. For many, it was a quiet, "low aphid" year with few reports of economic outbreaks within Iowa. Reflecting these low populations in the field, we observed fewer aphids within our suction traps (Figure 1) compared to the 2005 growing season when the traps collected nearly 100 times more aphids (Figure 2). For the last two years, the Iowa suction trap network (Figure 3) has been part of a larger network of suction traps located in nine states throughout ...


Dynamics And Structures Of Segregation In A Dense, Vibrating Granular Bed, Jin Sun, Francine Battaglia, Shankar Subramaniam Dec 2006

Dynamics And Structures Of Segregation In A Dense, Vibrating Granular Bed, Jin Sun, Francine Battaglia, Shankar Subramaniam

Mechanical Engineering Publications

We study the rise dynamics of a large particle in a granular bed under vertical vibration using molecular dynamics simulations. Systematic variation of the particle properties and external wall friction in the simulations shows that the large particle rising is very sensitive to external wall friction. The dynamical response of the granular bed with wall friction is shown to include an expansion stage and a compression stage within one cycle. With wall friction, large-scale force networks bearing larger-than-average forces are found in the compression stage. However, without wall friction, large strong force networks do not exist. The distribution of normal ...


Equilibrium Layoff As Termination Of A Dynamic Contract, Cheng Wang Dec 2006

Equilibrium Layoff As Termination Of A Dynamic Contract, Cheng Wang

Economics Working Papers (2002–2016)

In a dynamic model of the labor market with moral hazard, equilibrium layoff is modeled as termination of an optimal long-term contract. Termination, together with compensation (current and future), is used as an incentive device to induce worker efforts. I then use the model to study analytically the effects of a firing tax on termination and worker compensation and utility. There are three layers to the impact of a firing tax on layoff and worker utility. A higher firing tax could either reduce aggregate termination and increase worker utility, or increase aggregate termination and reduce worker utility, depending on the ...


Report To The Provost From The Task Force To Review Dialogues On Diversity, Task Force To Review Dialogues On Diversity, Iowa State University Dec 2006

Report To The Provost From The Task Force To Review Dialogues On Diversity, Task Force To Review Dialogues On Diversity, Iowa State University

Office of the Provost Reports

One of the action items arising from the work of the Campus Climate Implementation Team and the President’s Advisory Committee on Diversity was a request from the President to the Provost to review the Dialogues on Diversity course. The Provost instructed this Task Force to review the current Dialogues on Diversity course to determine how it should be continued for optimal impact and to determine whether it is appropriate to add a “Dialogues—Part 2” to the curriculum. The Provost’s charge to the Task Force also asked for recommendations that include details on the following components of the ...


Partially Identifying The Prevalence Of Health Insurance Given Contaminated Sampling Response Error, Brent Kreider Dec 2006

Partially Identifying The Prevalence Of Health Insurance Given Contaminated Sampling Response Error, Brent Kreider

Economics Working Papers (2002–2016)

This paper derives simple closed-form identification regions for the U.S. nonelderly population's prevalence of health insurance coverage in the presence of household reporting errors. The methods extend Horowitz and Manski's (1995) nonparametric analysis of contaminated samples for the case that the outcome is binary. In this case, draws from the alternative distribution (i.e., not the distribution of interest) might naturally be defined as response errors. The derived identification regions can dramatically reduce the degree of uncertainty about the outcome distribution compared with the contaminated sampling bounds. These regions are estimated using data from the Medical Expenditure ...


Modular Subclass Verification: Safely Creating Correct Subclasses Without Superclass Code, Clyde Ruby Dec 2006

Modular Subclass Verification: Safely Creating Correct Subclasses Without Superclass Code, Clyde Ruby

Computer Science Technical Reports

The documentation of object-oriented frameworks and class libraries needs to provide enough information so programmers can reason about the correctness of subclass methods without superclass code. Even though a superclass method satisfies its specification and behaves properly in the context of the superclass itself, a new subclass may cause that method to have unexpected or unverifiable behavior. For example, inherited superclass code can call down to subclass methods which may cause a superclass method to no longer satisfy its specification. Furthermore, without superclass code, downcalls can result in unverifiable side-effects. Aliasing can also result in unexpected or unverifiable side-effects. In ...


Potential Benefits Of Wetland Filters For Tile Drainage Systems: Impact On Nitrate Loads To Mississippi River Subbasins, William G. Crumpton, Greg A. Stenback, Bradley Allen Miller, Matt Helmers Dec 2006

Potential Benefits Of Wetland Filters For Tile Drainage Systems: Impact On Nitrate Loads To Mississippi River Subbasins, William G. Crumpton, Greg A. Stenback, Bradley Allen Miller, Matt Helmers

Ecology, Evolution and Organismal Biology Reports

The primary objective of this project was to estimate the nitrate reduction that could be achieved using restored wetlands as nitrogen sinks in tile-drained regions of the upper Mississippi River (UMR) and Ohio River basins. This report provides an assessment of nitrate concentrations and loads across the UMR and Ohio River basins and the mass reduction of nitrate loading that could be achieved using wetlands to intercept nonpoint source nitrate loads. Nitrate concentration and stream discharge data were used to calculate stream nitrate loading and annual flow-weighted average (FWA) nitrate concentrations and to develop a model of FWA nitrate concentration ...


Clear Creek Watershed: 150 Years Of Landscape Change, Andrew P. Rayburn, Lisa A. Schulte-Moore Dec 2006

Clear Creek Watershed: 150 Years Of Landscape Change, Andrew P. Rayburn, Lisa A. Schulte-Moore

Leopold Center Pubs and Papers

This fact sheet summarizes the findings of a Leopold Center Ecology Initiative grant (2004-E08) that looked at landscape changes in the Clear Creek watershed in east central Iowa since 1940 using aerial photos and historical records.


Potential Benefits Of Wetland Filters For Tile Drainage Systems: Impact On Nitrate Loads To Mississippi River Subbasins, William G. Crumpton, Greg A. Stenback, B. A. Miller, Matthew J. Helmers Dec 2006

Potential Benefits Of Wetland Filters For Tile Drainage Systems: Impact On Nitrate Loads To Mississippi River Subbasins, William G. Crumpton, Greg A. Stenback, B. A. Miller, Matthew J. Helmers

Agricultural and Biosystems Engineering Technical Reports and White Papers

The primary objective of this project was to estimate the nitrate reduction that could be achieved using restored wetlands as nitrogen sinks in tile-drained regions of the upper Mississippi River (UMR) and Ohio River basins. This report provides an assessment of nitrate concentrations and loads across the UMR and Ohio River basins and the mass reduction of nitrate loading that could be achieved using wetlands to intercept nonpoint source nitrate loads. Nitrate concentration and stream discharge data were used to calculate stream nitrate loading and annual flow-weighted average (FWA) nitrate concentrations and to develop a model of FWA nitrate concentration ...


Nontariff Barriers, John C. Beghin Dec 2006

Nontariff Barriers, John C. Beghin

CARD Working Papers

Nontariff barriers (NTBs) refer to the wide range of policy interventions other than border tariffs that affect trade of goods, services, and factors of production. Most taxonomies of NTBs include market-specific trade and domestic policies affecting trade in that market. Extended taxonomies include macro-economic policies affecting trade. NTBs have gained importance as tariff levels have been reduced worldwide. Common measures of NTBs include tariff-equivalents of the NTB policy or policies and count and frequency measures of NTBs. These NTB measures are subsequently used in various trade models, including gravity equations, to assess trade and/or welfare effects of the measured ...


Intra-Industry Trade, Multilateral Trade Integration, And Invasive Species Risk, Anh T. Tu, John C. Beghin Dec 2006

Intra-Industry Trade, Multilateral Trade Integration, And Invasive Species Risk, Anh T. Tu, John C. Beghin

CARD Working Papers

We analyze the linkage between protectionism and invasive species (IS) hazard in the context of two-way trade and multilateral trade integration, two major features of real-world agricultural trade. Multilateral integration includes the joint reduction of tariffs and trade costs among trading partners. Multilateral trade integration is more likely to increase damages from IS than predicted by unilateral trade opening under the classic Heckscher-Ohlin-Samuelson (HOS) framework because domestic production (the base susceptible to damages) is likely to increase with expanding export markets. A country integrating its trade with a partner characterized by relatively higher tariff and trade costs is also more ...


Aujeszky’S Disease, Iowa State University Center For Food Security And Public Health Dec 2006

Aujeszky’S Disease, Iowa State University Center For Food Security And Public Health

Center for Food Security and Public Health Technical Factsheets

Aujeszky’s disease (pseudorabies) is a highly contagious, economically significant disease of pigs. This viral infection causes central nervous system (CNS) signs and high mortality rates in young animals, and respiratory illness in older pigs. Other species may be infected when they come in contact with infected pigs, resulting in a universally fatal CNS disease. Aujeszky’s disease can result in trade restrictions from regions where it is endemic. Eradication programs are underway or have been successful in many countries. In the United States, all states are now considered to be free of the virus in domesticated swine, and a ...


Determining The Extent Of Frost Damage In Maize Seed Using The Tetrazolium Test, Mindy Devries, A. Susana Goggi Dec 2006

Determining The Extent Of Frost Damage In Maize Seed Using The Tetrazolium Test, Mindy Devries, A. Susana Goggi

Agronomy Publications

Frost damage in seed corn causes a significant decrease in seed quality. The tetrazolium (TZ) test has been used to identify types of physiological damage in several plant species, including frost damage in corn. Quantifying the amount of seed damaged by frost and the consequent loss of seed vigor are important for making management decisions. The current tetrazolium test procedures provide seed viability information but do not estimate seed vigor. In our project, the tetrazolium staining procedure was modified to allow separation of viable seeds into vigor categories. Vigor categories were defined based on Association of Official Seed Analysts (AOSA ...


Insect-Mediated Seed-Set Evaluation Of 21 Soybean Lines Segregating For Male Sterility At 10 Different Loci, E. Ortiz-Perez, Harry T. Horner, S. J. Hanlin, R. G. Palmer Dec 2006

Insect-Mediated Seed-Set Evaluation Of 21 Soybean Lines Segregating For Male Sterility At 10 Different Loci, E. Ortiz-Perez, Harry T. Horner, S. J. Hanlin, R. G. Palmer

Genetics, Development and Cell Biology Publications

The first requirement to establish a successful hybrid soybean program is the availability of a stable male-sterile, female-fertile system. Male sterility has been an important tool in soybean breeding programs to improve traits such as yield, seed-protein and seed-oil content, and seed size. However, improvement of seed-set per se on male-sterile plants has not been an important breeding objective. The evaluation of the out-crossing potential of the available male-sterile, female-fertile soybean lines is crucial to determine the future of hybrid soybean. The objective of this study was to evaluate seed-set among 21 soybean lines segregating for male sterility at 10 ...


Counterintuitive Density-Dependent Growth In A Long-Lived Vertebrate After Removal Of Nest Predators, Ricky-John Spencer, Fredric J. Janzen, Michael B. Thompson Dec 2006

Counterintuitive Density-Dependent Growth In A Long-Lived Vertebrate After Removal Of Nest Predators, Ricky-John Spencer, Fredric J. Janzen, Michael B. Thompson

Ecology, Evolution and Organismal Biology Publications

Examining the phenotypic and genetic underpinnings of life-history variation in long-lived organisms is central to the study of life-history evolution. Juvenile growth and survival are often density dependent in reptiles, and theory predicts the evolution of slow growth in response to low resources (resource-limiting hypothesis), such as under densely populated conditions. However, rapid growth is predicted when exceeding some critical body size reduces the risk of mortality (mortality hypothesis). Here we present results of paired, large-scale, five-year field experiments to identify causes of variation in individual growth and survival rates of an Australian turtle (Emydura macquarii) prior to maturity. To ...


Analysis Of Bulked And Redundant Accessions Of Brassica Germplasm Using Assignment Tests Of Microsatellite Markers, Von Mark V. Cruz, John D. Nason, Richard Luhman, Laura F. Marek, Randy C. Shoemaker, E. Charles Brummer, Candice A. C. Gardner Dec 2006

Analysis Of Bulked And Redundant Accessions Of Brassica Germplasm Using Assignment Tests Of Microsatellite Markers, Von Mark V. Cruz, John D. Nason, Richard Luhman, Laura F. Marek, Randy C. Shoemaker, E. Charles Brummer, Candice A. C. Gardner

Ecology, Evolution and Organismal Biology Publications

This study was conducted to determine if Brassica germplasm bulks created and maintained by the USDA-ARS North Central Plant Introduction Station (NCRPIS) were made with genetically indistinguishable component accessions and to examine newly identified putative duplicate accessions to determine if they can be bulked. Using ten microsatellite primer pairs, we genotyped two bulks of B. rapa L. ssp. dichotoma (Roxb.) Hanelt comprising four accessions and three bulks of B. rapa L. ssp. trilocularis (Roxb.) Hanelt comprising fourteen accessions, as well as four pairs of putatively duplicate accessions of B.␣napus L. Assignment tests on ten individual plants per accession were ...


Amblyomma Hebraeum, Iowa State University Center For Food Security And Public Health Dec 2006

Amblyomma Hebraeum, Iowa State University Center For Food Security And Public Health

CFSPH fichas de las enfermedades

Amblyomma hebraeum es una garrapata dura que infesta al ganado y a los animales silvestres. También pica a los humanos. Las largas piezas bucales de Amblyomma hacen que esta garrapata sea difícil de quitar en forma manual; estas garrapatas también dejan grandes heridas que se pueden infectar con bacterias o infestar con gusanos barrenadores. A. hebraeum puede transmitir Ehrlichia ruminantium (anteriormente denominada Cowdria ruminantium), que es el agente causal de la cowdriosis. Esta garrapata también es portadora de Rickettsia africae, que causa la fiebre por picadura de la garrapata africana, una zoonosis emergente en zonas rurales del África subsahariana y ...


Amblyomma Hebraeum, Iowa State University Center For Food Security And Public Health Dec 2006

Amblyomma Hebraeum, Iowa State University Center For Food Security And Public Health

Center for Food Security and Public Health Technical Factsheets

Amblyomma hebraeum is a hard tick that infests livestock and wildlife. It also bites humans. The long mouthparts of Amblyomma ticks make them difficult to remove manually; these ticks also leave large wounds that may become infected by bacteria or infested by screwworms. A. hebraeum can transmit Ehrlichia ruminantium (formerly Cowdria ruminantium), the agent of heartwater. This tick also carries Rickettsia africae, the agent of African tick-bite fever, an emerging zoonosis in rural sub-Saharan Africa and the Caribbean.


Creating Offshore-Ready It Professionals: A Global Perspective And Strong Collaborative Skills Are Needed, Kevin P. Scheibe, Brian E. Mennecke, Christopher W. Zobel Dec 2006

Creating Offshore-Ready It Professionals: A Global Perspective And Strong Collaborative Skills Are Needed, Kevin P. Scheibe, Brian E. Mennecke, Christopher W. Zobel

Supply Chain and Information Systems Publications

Outsourcing of IT functions has become a widespread corporate practice, which has naturally led to concerns among IT works about how this affects their jobs. The issue is complex, and many companies are bringing their IT functions back inhouse. In light of this complexity, what skills do IT workers need to be com-petitive? We address this question first by reviewing the literature and then by examining two corporate case studies that have dealt with outsourcing issues. Based on this view of outsourcing, we discuss the skills that can provide a com-petitive advantage in the current environment.


Enfermedad De Aujeszky, Iowa State University Center For Food Security And Public Health Dec 2006

Enfermedad De Aujeszky, Iowa State University Center For Food Security And Public Health

CFSPH fichas de las enfermedades

La enfermedad de Aujeszky (seudorrabia) es una enfermedad altamente contagiosa que afecta a los cerdos, de gran importancia económica. Esta infección viral afecta el sistema nervioso central (SNC), presenta índices de mortalidad elevados en los animales jóvenes; en cerdos adultos produce afecciones respiratorias. Otras especies pueden infectarse al entrar en contacto con cerdos infectados, dando como resultado una enfermedad del SNC de alta mortalidad. Esta enfermedad puede ocasionar restricciones comerciales para las regiones endémicas. Actualmente existen programas de erradicación que han resultado exitosos en muchos países. Hoy en día, se considera que en Estados Unidos los cerdos domésticos están libres ...


Measurement Of High-P(T) Single Electrons From Heavy-Flavor Decays In P+P Collisions At Root S=200 Gev, Andrew Adare, Nathan C. Grau, John C. Hill, John G. Lajoie, Alexandre Lebedev, Craig Ogilvie, H. Pei, Marzia Rosati, S. Skutnik, Carla Vale, Et Al., Phenix Collaboration Dec 2006

Measurement Of High-P(T) Single Electrons From Heavy-Flavor Decays In P+P Collisions At Root S=200 Gev, Andrew Adare, Nathan C. Grau, John C. Hill, John G. Lajoie, Alexandre Lebedev, Craig Ogilvie, H. Pei, Marzia Rosati, S. Skutnik, Carla Vale, Et Al., Phenix Collaboration

Physics and Astronomy Publications

The momentum distribution of electrons from decays of heavy flavor (charm and bottom) for midrapidity |y|< 0.35 in p+p collisions at s=200 GeV has been measured by the PHENIX experiment at the BNL Relativistic Heavy Ion Collider over the transverse momentum range 0.3 < p(T)< 9 GeV/c. Two independent methods have been used to determine the heavy-flavor yields, and the results are in good agreement with each other. A fixed-order-plus-next-to-leading-log perturbative QCD calculation agrees with the data within the theoretical and experimental uncertainties, with the data/theory ratio of 1.71 +/- 0.02(stat)+/- 0.18(sys) for 0.3 < p(T)< 9 GeV/c. The total charm production cross section at this energy has also been deduced to be sigma(cc)=567 +/- 57(stat)+/- 193(sys) mu b.


Restoring Perennial Cover And Ecological Function To Corn Belt Landscapes: The Iowa Farmer's Perspective, Ryan C. Atwell, Lisa A. Schulte, Lynne M. Westphal Dec 2006

Restoring Perennial Cover And Ecological Function To Corn Belt Landscapes: The Iowa Farmer's Perspective, Ryan C. Atwell, Lisa A. Schulte, Lynne M. Westphal

Natural Resource Ecology and Management Publications

The tallgrass prairie ecosystem of the North American Corn Belt region has experienced major alteration of hydrologic systems, nearly complete loss of natural perennial vegetation, and concomitant decline in species due to agricultural intensification. In this working landscape, restoration to pre-European settlement conditions is doubtful. However, current research posits that restoring strategically placed patches of perennial cover across agricultural watersheds, such as stream buffers, wetlands and prairies, as well as instituting more diverse cropping rotations, could bolster current ecological function and enhance the conservation of species. These studies also suggest that such restoration might increase socio-economic resilience in the region ...


Monitoring Responses By Use Of Five-Color Flow Cytometry In Subsets Of Peripheral T Cells Obtained From Cattle Inoculated With A Killed Mycobacterium Avium Subsp Paratuberculosis Vaccine, Ratree Platt, James A. Roth, Ryan L. Royer, Charles O. Thoen Dec 2006

Monitoring Responses By Use Of Five-Color Flow Cytometry In Subsets Of Peripheral T Cells Obtained From Cattle Inoculated With A Killed Mycobacterium Avium Subsp Paratuberculosis Vaccine, Ratree Platt, James A. Roth, Ryan L. Royer, Charles O. Thoen

Veterinary Microbiology and Preventive Medicine Publications

Objective—To monitor by use of 5-color flow cytometry the antigen-specific responses of subsets of peripheral T cells in cattle inoculated with a killed Mycobacterium avium subsp paratuberculosis (MAP) vaccine and to compare results with those for 2 established cell-mediated immunity assays.

Animals—45 female Holstein cattle with negative results for MAP in skin tests conducted at time of inoculation with MAP.

Procedures—Cattle were allocated to 4 groups. Cattle of group 1 (n = 12) were 0 to 3 months old and inoculated with a killed MAP vaccine. The 10 cattle of group 2 were the same age as those ...