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Untemplated Oligoadenylation Promotes Degradation Of Risc-Cleaved Transcripts, Fadia Ibrahim, Jennifer Rohr, Won-Joong Jeong, Jennifer Hesson, Heriberto D. Cerutti
Untemplated Oligoadenylation Promotes Degradation Of Risc-Cleaved Transcripts, Fadia Ibrahim, Jennifer Rohr, Won-Joong Jeong, Jennifer Hesson, Heriberto D. Cerutti
Center for Plant Science Innovation: Faculty and Staff Publications
In the best-characterized mechanism of RNAmediated silencing, small interfering RNAs (siRNAs), incorporated into the RNA-induced silencing complex (RISC), guide the endonucleolytic cleavage of complementary RNAs (1). In Drosophila melanogaster, these RISC-generated products are eventually degraded by exoribonucleases: Xrn1, a 5′-to-3′ exonuclease, and exosome, a 3′-to-5′ multisubunit exonuclease (2). Interestingly, in Arabidopsis thaliana and in mammals, an oligouridine or oligoadenine [oligo(U/A)] tail is added to the 5′ RNA fragments resulting from microRNA-directed cleavage (3). However, the biological role of this tail remains unclear.
United States Patent Application Publication: Counter Selection Strategy For Gram-Negative Bacteria, Stephen K. Farrand, Paul E. Staswick, Thomas E. Clemente
United States Patent Application Publication: Counter Selection Strategy For Gram-Negative Bacteria, Stephen K. Farrand, Paul E. Staswick, Thomas E. Clemente
Center for Plant Science Innovation: Faculty and Staff Publications
A Gram-negative bacterium useful for genetically engineering plants is provided. The Gram-negative bacterium contains, as part of genome, an inducible regulatory sequence operatively linked to a nucleotide sequence encoding a levansucrase. Alternatively, the Gram-negative bacterium comprises a recombinant nucleic acid construct containing an inducible regulatory sequence operatively linked to a nucleotide sequence encoding a levansucrase. Also provided are recombinant nucleic acid constructs comprising an inducible regulatory sequence operatively coupled to a nucleotide sequence encoding a levansucrase and a method for transforming plants using the Gram-negative bacterium of the present invention.
Pseudomonas SyringaeHrpj Is A Type Iii Secreted Protein That Is Required For Plant Pathogenesis, Injection Of Effectors,And Secretion Of The Hrpz1 Harpin, Zheng Qing Fu, Ming Guo, James R. Alfano
Pseudomonas SyringaeHrpj Is A Type Iii Secreted Protein That Is Required For Plant Pathogenesis, Injection Of Effectors,And Secretion Of The Hrpz1 Harpin, Zheng Qing Fu, Ming Guo, James R. Alfano
Center for Plant Science Innovation: Faculty and Staff Publications
The bacterial plant pathogen Pseudomonas syringae requires a type III protein secretion system (TTSS) to cause disease. The P. syringae TTSS is encoded by the hrp-hrc gene cluster. One of the genes within this cluster, hrpJ, encodes a protein with weak similarity to YopN, a type III secreted protein from the animal pathogenic Yersinia species. Here, we show that HrpJ is secreted in culture and translocated into plant cells by the P. syringae pv. tomato DC3000 TTSS. A DC3000 hrpJ mutant, UNL140, was greatly reduced in its ability to cause disease symptoms and multiply in Arabidopsis thaliana …
On The Origin And Functions Of Rna-Mediated Silencing: From Protists To Man, Heriberto D. Cerutti, Juan Casas-Mollano
On The Origin And Functions Of Rna-Mediated Silencing: From Protists To Man, Heriberto D. Cerutti, Juan Casas-Mollano
Center for Plant Science Innovation: Faculty and Staff Publications
Double-stranded RNA has been shown to induce gene silencing in diverse eukaryotes and by a variety of pathways. We have examined the taxonomic distribution and the phylogenetic relationship of key components of the RNA interference (RNAi) machinery in members of five eukaryotic supergroups. On the basis of the parsimony principle, our analyses suggest that a relatively complex RNAi machinery was already present in the last common ancestor of eukaryotes and consisted, at a minimum, of one Argonaute-like polypeptide, one Piwi-like protein, one Dicer, and one RNA-dependent RNA polymerase. As proposed before, the ancestral (but non-essential) role of these components may …
Review Of Polarity In Plants, Annual Plant Reviews, Volume 12, Steven D. Harris
Review Of Polarity In Plants, Annual Plant Reviews, Volume 12, Steven D. Harris
Center for Plant Science Innovation: Faculty and Staff Publications
Polarity is a fundamental property of all cells. The molecular mechanisms that underlies the establishment and maintenance of cellular polarity have been intensively studied in animal cells and yeast-like fungi. These studies reveal an emerging general principle whereby positional information is relayed to the cytoskeleton and other morphogenetic functions by conserved signaling pathways. Comparatively less attention has been directed toward the molecular basis of polarized growth in plant cells and tissues. This volume, Polarity in Plants, provides a comprehensive review of the topic that should satisfy both hardcore aficionados of plant cell and developmental biology as well as curious investigators …
A Highly Efficient Transient Protoplast System For Analyzing Defence Gene Expression And Protein–Protein Interactions In Rice, Songbiao Chen, Lizen Tao, Lirong Zeng, Miguel E. Vega-Zanchez, Kenji Umemura, Guo-Liang Wang
A Highly Efficient Transient Protoplast System For Analyzing Defence Gene Expression And Protein–Protein Interactions In Rice, Songbiao Chen, Lizen Tao, Lirong Zeng, Miguel E. Vega-Zanchez, Kenji Umemura, Guo-Liang Wang
Center for Plant Science Innovation: Faculty and Staff Publications
The transient assay system based on mesophyll or cultured cell-derived protoplasts has been exploited in several plant species and has become a powerful tool for rapid gene functional analysis and biochemical manipulations. However, the system has not been widely used in rice owing to the difficulties in large-scale isolation of viable rice protoplasts from leaves or suspension-cultured cells. Here, we describe a significantly improved method to isolate a large number of protoplasts from stem and sheath tissues of both young and mature plants. High-level coexpression of multiple constructs and efficient suppression of exogenous and endogenous genes were observed in the …