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Immunoglobulin Κ Chain Allotypes (Km) In Onchocerciasis, Janardan P. Pandey, Lynne H. Elson, Susan E. Sutherland, Ronald H. Guderian, Edmundo Araujo, Thomas B. Nutman

MUSC Faculty Journal Articles

GM and KM allotypes, powerful tools for genetic characterization of human populations, have been shown to play an important role in genetic predisposition to some infectious diseases. Two diverse racial groups-Afro-Ecuadorians and Amerindians-living in a single restricted geographical area of Ecuador, appear to have different risk factors for acquisition and clinical expression of onchocerciasis, a disease caused by the filarial parasite Onchocerca volvulus. In this study, GM and KM allotypes were determined in 25 Afro-Ecuadorians and 24 Amerindians infected with Onchocerca volvulus (INF) and in putative immune individuals (PI). In Afro-Ecuadorians, the frequency of the homozygous KM 3 phenotype was …

Mutation Or Deletion Of The Saccharomyces Cerevisiae Rat3/Nup133 Gene Causes Temperature-Dependent Nuclear Accumulation Of Poly(A)+ Rna And Constitutive Clustering Of Nuclear Pore Complexes, Ou Li, Catherine V. Heath, David C. Amberg, Thomas C. Dockendorff, Connie S. Copeland, Michael Snyder, Charles N. Cole

Dartmouth Scholarship

To identify genes whose products play potential roles in the nucleocytoplasmic export of messenger RNA, we isolated temperature-sensitive strains of Saccharomyces cerevisiae and examined them by fluorescent in situ hybridization. With the use of a digoxigen-tagged oligo-(dT)50 probe, we identified those that showed nuclear accumulation of poly(A)+ RNA when cells were shifted to the nonpermissive temperature. We describe here the properties of yeast strains bearing the rat3-1 mutation (RAT-ribonucleic acid trafficking) and the cloning of the RAT3 gene. When cultured at the permissive temperature of 23 degrees C, fewer than 10% of cells carrying the rat3-1 allele showed nuclear accumulation …

Ms 089 Guide To Robert K. Blair, Md Papers (1944-1983), James V. Neel (1915-2000)

Manuscript Finding Aids

The James V. Neel papers contains incoming and outgoing Atomic Bomb Casualty Commission communication and correspondence, Japan lectures, Nagasaki study of Metal Ret. Children, ABCC memoranda and reports, various conference information, genetics and vital statistics, congenital and/or hereditary abnormalities in Japanese and Caucasians, quarterly reports submitted from Japan, monthly reports, genetics and research information, Kitamura program, consultants correspondence, staff correspondence, photocopy of manuscript, and other prints and photos related to the work and research of Dr. James V. Neel. See more informaon at MS 089.

Protein-Peptide Interactions Analyzed With The Yeast Two-Hybrid System, Meijia Yang, Zining Wu, Stanley Fields

Dartmouth Scholarship

The yeast two-hybrid system was used to screen a library of random peptides fused to a transcriptional activation domain in order to identify peptides capable of binding to the retinoblastoma protein (Rb). Seven peptides were identified, allof which contain the Leu-X-Cys-X-Glu motif found in Rb-binding proteins, although their activity in the yeast assay variedover a 40-fold range. Mutagenesis of the DNA encoding two of these peptides followed by screening in the two-hybrid systemallowed the delineation of residues apart from the invariant Leu, Cys and Glu that affect binding to Rb. Binding affinities of a peptide and one of its variants …

Genetic Information And Health Insurance: State Legislative Approaches, Karen H. Rothenberg

Faculty Scholarship

No abstract provided.

An Analysis Of The Primordial Soup Hypothesis With Respect To Dna Structure And The Genetic Code, Jennifer A. Hart

Masters Theses

The primordial soup hypothesis is currently the most popular origin of life theory. This hypothesis suggests that DNA and self-replicating organisms were formed out of a warm, aqueous environment on the surface of the prebiotic earth. However, recent studies have shown that the primordial soup hypothesis is not only lacking in factual support, it is also internally flawed. The primordial soup hypothesis is statistically impossible, and it does not adequately account for the origin of the specified complexity of the genetic code. The very nature of the genetic code requires an intelligence source. Universal experience shows that specified complexity is …

A Phylogenetic Analysis Of Vertebrate And Invertebrate Notch-Related Genes, Eleanor M. Maine, James L. Lissemore, William T. Starmer

Biology - All Scholarship

Members of the Notch gene family are thought to mediate inductive cell-cell interactions during develop ment of a wide variety of vertebrates and invertebrates. These genes encode transmembrane proteins that appear to act as receptors and contain three repeated sequence motifs. Two of these motifs (an epidermal growth factor like sequence and a cdc10/SW16/ankyrin sequence) have been found in a large number of unrelated proteins, while the third motif (a lin-12/Notch/glp-1 sequence) is unique to proteins of the Notch family. We present a phylogenetic analysis of 17 Notch-related genes from eight species that has implications as to the origins and …

The Gtp-Bound Form Of The Yeast Ran/Tc4 Homologue Blocks Nuclear Protein Import And Appearance Of Poly(A)+ Rna In The Cytoplasm., Gabriel Schlenstedt, Claudio Saavedra, Jonathan D. Loeb, Charles N. Cole, Pamela A. Silver

Dartmouth Scholarship

Ran/TC4, a Ras-like GTP-binding protein, and its nucleotide exchanger, RCC1, have been implicated in control of protein movement into the nucleus and cytoplasmic accumulation of mRNA. Saccharomyces cerevisiae contains two homologues of the mammalian Ran/TC4, encoded by the GSP1 and GSP2 genes. We have constructed yeast strains that overproduce either wild-type Gsp1 or a form of Gsp1 with glycine-21 converted to valine (Gsp1-G21V), which we show stabilizes the GTP-bound form. Cells producing Gsp1-G21V have defects in localization of nuclear proteins; nuclear proteins accumulate in the cytoplasm following galactose induction of Gsp1-G21V. Similarly, cells producing Gsp1-G21V retain poly(A)+ RNA in their …

G95-1271 Mastitis Is A Disease -- Control Is An Everyday Task, Gerald R. Bodman, Duane N. Rice

University of Nebraska-Lincoln Extension: Historical Materials

A comprehensive mastitis control program will effectively control infections caused by both environmental and contagious pathogens.

Herd mastitis problems can be caused by both environmental and contagious pathogens (disease-causing organisms). These problems may occur separately or simultaneously. Various testing techniques are necessary to determine the type of causative organism and the infection level of the herd or individual cow. Appropriate tests include the CMT (California Mastitis Test), milk market somatic cell count (SCC), Dairy Herd Improvement Association (DHIA) SCC and laboratory cultures of milk from infected cows. Depending upon test results, mastitis control strategies may need to be changed to …

Carbon Allocation And Partitioning In Aspen Clones Varying In Sensitivity To Tropospheric Ozone, M.D. Coleman, R.E. Dickson, J.G. Isebrands, D.F. Karnosky

Aspen Bibliography

No abstract provided.