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Single-Fluorophore Sensors For Mechanical Force In Living Cells, Sarah Kricheff

Honors Scholar Theses

Mechanotransduction is the process by which a mechanical stimulus is converted to a cellular signal. This process is heavily influential of cell morphology, differentiation, and behavior. However, altered levels of mechanical stimuli are also found in many pathological contexts. For example, cancerous cells have stiffer surrounding tissue than healthy cells, and research suggests that this alters cell behavior and promotes metastasis. Despite these findings, the cellular processes behind these signaling alterations remain widely unknown. Understanding these cascades is critical, as involved proteins can give us a deeper understanding of the role of mechanotransduction, and certain proteins can potentially be targeted …

The Genetic Influence On Subjective Well-Being: A Review Of The Current Knowledge On The Role Of Genetics On Our Sense Of Subjective Well-Being And The Implications It Has For Future Research In Improving Well-Being At Both A Population And Individual Level., Abhishek Gupta

Honors Scholar Theses

This thesis project explores the genetic underpinnings of one of the most cherished attributes in the world, well-being.¹ Specifically, it attempts to understand the influence of the genome on subjective, or experienced, well-being. An investigation was conducted into current literature concerning both the structure of measurement devices of well-being as well as association studies to determine the scope of the correlation that exists between the genome and well-being and identify genetic findings of interest. Ultimately, being able to provide evidence of causality between the genome and sense of well-being at this iteration of well-being and genome research is limited, …

George Argus’S List Of Salix References, Yulia Kuzovkina-Eischen

Plant Science Articles

This bibliographical list, containing more than 3600 publications related to the genus Salix, was compiled by Dr. Argus during his long scientific career.

Spirituality As A Moderator Between Ptss And Cardiovascular Reactivity, Kriti Sharda

Honors Scholar Theses

Posttraumatic stress symptoms (PTSS) can be distressing and produce robust cardiovascular symptoms, such as increased heart rate and blood pressure, which have been implicated in higher risk of developing cardiovascular disease (CVD). Identifying factors that may reduce symptoms may suggest therapeutic strategies. One such potential factor is spirituality, given that spirituality is associated with both reducing PTSS and with preventing or improving CVD. We sampled 63 young college women who indicated being exposed to unwanted sexual contact. We asked them to write about their experience while we took heart rate (HR), systolic blood pressure (SBP) and diastolic blood pressure (DBP) …

Study Of A Putative Niche Adapting Operon In Microbes Inhabiting The Gut Of Blood Digesting Animals, Marlene Abouaassi

University Scholar Projects

The sialic acid utilization (SAU) operon is a horizontally acquired gene set that allows bacteria to utilize sialic acid as an alternate source of carbon and nitrogen in the guts of blood eating animals. Sialic acid often occurs as a terminal sugar in complex glycoproteins. It functions in cell signaling and adhesion. Sialic acid is an important component of the cellular envelope of animals. Some microorganisms have evolved to decorate their own surface with sialic acid to evade the host’s immune response (molecular mimicry). The SAU operon encodes enzymes that hydrolyze sialic acid from glycoproteins, transport sialic acid into the …

Study Of A Putative Niche Adapting Operon In Microbes Inhabiting The Gut Of Blood Digesting Animals, Marlene Abouaassi

Honors Scholar Theses

The sialic acid utilization (SAU) operon is a horizontally acquired gene set that allows bacteria to utilize sialic acid as an alternate source of carbon and nitrogen in the guts of blood eating animals. Sialic acid often occurs as a terminal sugar in complex glycoproteins. It functions in cell signaling and adhesion. Sialic acid is an important component of the cellular envelope of animals. Some microorganisms have evolved to decorate their own surface with sialic acid to evade the host’s immune response (molecular mimicry). The SAU operon encodes enzymes that hydrolyze sialic acid from glycoproteins, transport sialic acid into the …

Contributions Of Gyra And Parc Mutations And Qnrs2 Acquisition To Ciprofloxacin Resistance In Aeromonas Veronii Hm21, Daniel J. Silverstein

Honors Scholar Theses

In recent years, ciprofloxacin resistant (CpR) Aeromonas veronii and A. hydrophila strains have been isolated from the wounds of patients receiving leech therapy. Genome comparisons of these CpR isolates revealed the presence of chromosomal mutations in gyrA and parC as well as the gain of qnrS2 on either a large, 34 kb, conjugatable, low-copy plasmid, pAv42, or on a small, 6.8 kb, high-copy plasmid, pAh1471. The minimum inhibitory concentration, MIC, for Cp of these clinical isolates ranged from 1 to ≥32 µg/mL and some harbored a qnrS2 containing plasmid. We wanted to assess the contributions of these factors in an …

3,4-Dihydroxyphenylacetate 2,3-Dioxygenase Modulates The Ability Of Enterobacter Hormaechei To Induce Cryptococcus Neoformans Melanization, Amy Nelson

Honors Scholar Theses

In this study, we performed transposon mutagenesis to create a library of Enterobacter hormaechei mutants and developed a melanization assay to identify mutants that could not induce the typical melanization phenotype in Cryptococcus neoformans. Relevant phenotype labels included: “hypomelanizer”, a total lack of melanization; “hypermelanizer”, an increased melanization; or “abnormal”, an increased melanization with complete color change of the assay plate to a reddish-brown. Genomic sequencing of 47 mutants and bioinformatic analysis allowed us to pinpoint the transposon insertion site in each E. hormaechei mutant to identify the genes that were affected. A single mutant that induced the abnormal …

Kcnq2 Localization In The Brainstem, Christina Valera

Honors Scholar Theses

KCNQ2 channels are potassium channels that serve to control neuronal excitability. Loss of function mutations in these channels are known to cause various forms of epilepsy. Recently, KCNQ2 R201C and R201H gain of function mutations have been shown to exhibit an exaggerated startle response and other unique phenotypes uncharacteristic of epilepsy. These phenotypes resemble hyperekplexia, a condition in which glycine neurotransmission in the spinal cord and brainstem is affected. While KCNQ2 has widespread localization throughout the brain, its presence in the brainstem remains unknown. We used immunostaining to determine the localization of KCNQ2 in the vagus nerve and hypoglossal nerve …

Using Machine Learning To Conduct A Detailed Behavioral Analysis In An Appetitive Social Learning Task, Thomas Shao

Honors Scholar Theses

Learning by watching others, or observational learning, is important for social development and survival. However, not much is known about the brain mechanisms underlying this type of learning. Since the 1960s, observational learning has been widely studied in humans, but developing and analyzing experiments for animals has been challenging. Here, I explore observational learning using a novel paradigm while performing an analysis that involves tracking the rats using an active learning paradigm called DeepLabCut. In this novel paradigm, customized operant conditioning chambers are used for the rats to observe and learn from another animal repeatedly on multiple trials each day. …

Tyrr Regulates The Ability Of Enterobacter Hormaechei To Induce Melanin Production In Cryptoccus Neoformans, Ally Watson

Honors Scholar Theses

The yeast Cryptococcus neoformans is present in the microbiota of many plants and animals and produces melanin, a compound that protects cells against UV light and promotes virulence. Melanin production can be induced by exogenous tyrosine, homogentisic acid, catecholamines, or their precursors such as L-DOPA, all of which may be produced by bacteria. The goal of this study was to investigate the interaction between Enterobacter hormaechei (a mosquito gut isolate) and C. neoformans with respect to the production of melanin by C. neoformans in the presence of E. hormaechei, potentially due to the production of L-DOPA or other metabolites …

The Anti-Proliferative Effects Of Methotrexate And Novel Ucp1162 On Acute Myeloid Leukemia Cell Lines, Jacqueline Klepinger, Charles Giardina, Didem Ozcan

Honors Scholar Theses

Cancer cells proliferate at rapid rates due to the aberrant activity of proteins involved in regulating the cell cycle. This characteristic allows mutated cancer cells to spread and metastasize, causing lesions to form throughout the body. Two treatment conditions, one classical antifolate methotrexate (MTX) and non-classical, novel antifolate UCP1162, were tested on a panel of acute myeloid leukemia (AML) cell lines to determine if UCP1162 has higher anti-proliferative activity. High dose MTX is used as a first line chemotherapy in common childhood malignancies such as acute lymphoid leukemia (ALL). Methotrexate is excluded from acute myeloid leukemia (AML) treatments based on …

Remapping Of Place Cells In The Dorsal And Ventral Hippocampus In Response To Environmental Change, Aditi Anam

Honors Scholar Theses

The hippocampus is an important brain structure involved in memory and navigation of both rats and humans. Neurons in the hippocampus can be “spatially” tuned, meaning they fire in specific physical locations. These spatially tuned cells are referred to as place cells. Collectively, they are thought to provide a map-like representation of the environment around us. If the environment is changed, some place cells can adjust by “remapping”, or altering their firing patterns. There are multiple sub-regions within the hippocampus. During experiments, cells were recorded simultaneously from the dorsal and ventral hippocampus of rats running on a track for food …

Rapid Antibiotic Susceptibility Testing Platform For Direct Clinical Samples, Terrance Zhang

Honors Scholar Theses

Infectious diseases and septicemia are two of the major causes of death in the U.S., necessitating rapid treatment of septic patients with proper, efficacious antibiotics. Unfortunately, the emergence and spread of multidrug-resistant bacteria are continuously being aggravated by an abuse in antibiotic prescription at a clinical and agricultural level. It is known that antibiotic resistance evolves through the sequential accumulation of multiple mutations in bacteria, which is accentuated by prolonged exposure of bacteria to ineffective antibiotics when implementing traditional septicemia treatment. The goal of this project is to develop a novel, easy-to-use AST platform for rapid antimicrobial susceptibility profiling to …

Detection Of Tinnitus In Cba/Caj Mice Using The Active Avoidance Shuttle Box Test, Grace Nichols

University Scholar Projects

Tinnitus is a neurological condition that involves the perception of a sound that is not actually there. Individuals affected with tinnitus describe the condition as a ringing, buzzing, or whooshing sound in their ears. One-third of the population is estimated to have tinnitus, and for many individuals, the condition negatively impacts quality of life. Difficulty falling asleep, trouble with hearing and concentrating, and in some cases, depression, have all been observed to occur with tinnitus.

Tinnitus has been extensively studied in animals, and behavioral tests are the primary method of evaluating the presence of tinnitus in animals. This study evaluates …

Genetic Analysis Of Flower Color Differences Between A Hummingbird-Pollinated And A Self-Pollinated Monkeyflower (Mimulus) Species, Caitlin Foster

University Scholar Projects

Flower color plays an important role in pollinator discrimination and speciation. Understanding the genetic contributions to flower color differences between two closely related species, Mimulus cardinalis and Mimulus parishii, can improve understanding of how they developed different pollination syndromes and diverged from a recent common ancestor. M. cardinalis is hummingbird-pollinated and has large, bright red flowers while M. parishii is self-pollinated and has small, pale pink flowers. An F2 hybrid population between these two species was created to establish a platform for analysis of the genetic architecture controlling the differences in anthocyanin pigmentation. Statistical analysis of anthocyanin concentration distribution …

Mechanisms Of Statin Effects On Muscle And Neuronal Proteostasis, Daniel Yu

University Scholar Projects

Statins are widely prescribed and used chronically, but we know little about the effects on long-term protein homeostasis during stress and aging. Our aim was to quantify the effect of statins on stress-induced protein damage. We administered atorvastatin in a dose-response curve in Caenorhabditis elegans under naïve control conditions and in conditions of hypertonic and heat stress known to induce muscle damage measurable as countable puncta in a polyglutamine aggregation model of damage. We observed that there is significant polyglutamine aggregation variability among worms at baseline and thus further study requires within experiment baseline controls, per worm. Our results are …

Reorganization Of The Human Ventricular-Subventricular Zone Neural Stem Cell Niche In Fetal-Onset Hydrocephalus, Saurabh Kumar

University Scholar Projects

Fetal-onset hydrocephalus is a relatively common birth defect occurring in 1-2 cases per thousand births and is characterized by abnormally expanded brain ventricles. Current diagnosis for this complex pathology often involves relatively simple metrics and heavy reliance on clinician experience over objective measures. Those affected often suffer chronic headaches and cognitive deficits and may present with a bulging skill. Shunting is considered the standard treatment for communicating hydrocephalus (i.e. cerebrospinal fluid flow is physically unobstructed in the ventricular system). Shunting remains a highly invasive procedure often performed during the critical period of infancy and has a high failure rate of …

Microglia-Neuron Interactions In A Mouse Model Of Low Grade Neuroepithelial Tumors, Veolette Hanna

Honors Scholar Theses

Microglia are the macrophages of the brain and spinal cord, playing an important role in the immune response to disease states of the nervous system. This study conducts an investigation on the activity of microglia in response to low grade neuroepithelial tumors. Using mouse models and microglial markers, a qualitative and quantitative analysis of microglia activation, migration, and invasion within the brain cortex during early stages of tumor development was conducted. It was found that the presence of a low grade neuroepithelial tumor in the cortex of one hemisphere of the brain causes significant microglia activation in comparison to the …

Identification And Analysis Of Feather Degrading Bacteria: A Search For Keratinase Genes, Nehal Navali

Honors Scholar Theses

Over two million tons of feather waste is generated annually by the poultry industry, the majority of which goes into landfills due to the difficulty of degrading its major component keratin. Although a portion of feather waste is eliminated via incineration or chemical treatment, the use of Feather Degrading Bacteria (FDB) has been proposed as a cheap and eco-friendly alternative. FDBs have been consistently isolated from the feather microbiome of birds and contain genes coding for the specialized protein keratinase which is able to degrade feathers. By doing so, feather waste, which is rich in nutrients, can be repurposed as …

Toxicity Analysis Of 2’-Deoxyguanosine-N2-6-Aminopyrene And 2’-Deoxyguanosine-N2-8-Aminopyrene In Escherichia Coli, Emily Janeiro

Honors Scholar Theses

Cancer is a disease that stems from genomic errors that are not corrected properly by cellular repair mechanisms. Errors are more likely to form when organisms are subjected to DNA damage by mutagenic compounds. 1-Nitropyrene, a nitrated polycyclic aromatic hydrocarbon (nitro-PAH), has been shown to be a potent mutagen that causes cancer. Nitro-PAHs can arise from diesel exhaust products in the environment. Out of all nitro-PAHs, 1-nitropyrene is found in largest quantities in the environment. This poses a great need to study its effects biochemically in order to address its toxicity in DNA. Other nitropyrene derivatives, including 1,6-dinitropyrene and 1,8-dinitropyrene, …

Detection Of Tinnitus In Cba/Caj Mice Using The Active Avoidance Shuttle Box Test, Grace Nichols

Honors Scholar Theses

Tinnitus is a neurological condition that involves the perception of a sound that is not actually there. Individuals affected with tinnitus describe the condition as a ringing, buzzing, or whooshing sound in their ears. One-third of the population is estimated to have tinnitus, and for many individuals, the condition negatively impacts quality of life. Difficulty falling asleep, trouble with hearing and concentrating, and in some cases, depression, have all been observed to occur with tinnitus.

Tinnitus has been extensively studied in animals, and behavioral tests are the primary method of evaluating the presence of tinnitus in animals. This study evaluates …

Optical Clearing Reveals Tnbs-Induced Morphological Changes Of Vglut2-Positive Nerve Endings In The Colorectum, Shivam Patel

Honors Scholar Theses

Sensitization of colorectal afferents and colorectal hypersensitivity have been observed in a mouse model of post-infectious irritable bowel syndrome via intracolonic treatment of 2,4,6-trinitrobenzenesulfonic acid (TNBS). In this study, we investigated the distribution and morphology of microscopic colorectal afferent endings before and after intracolonic treatment of TNBS. We genetically labeled predominantly extrinsic colorectal afferents using the vesicular glutamate transporter type 2 (VGLUT2) promoter. Then, we used an optical tissue clearing method of whole-mount colorectum to image labeled VGLUT2-nerve endings that are otherwise obscured in untreated samples. We used vector path tracing to quantify the density and degree of curliness of …

The Effects Of The Atypical Dopamine Uptake Inhibitor Ce-158 On Extracellular Dopamine In The Nucleus Accumbens, Julia Neri

Honors Scholar Theses

Major Depressive Disorder (MDD) is characterized by symptoms such as cognitive dysfunctions, inflammatory changes, and motivational symptoms such as amotivation, fatigue, and anergia. While depressed people are commonly treated by traditional antidepressants such as serotonin reuptake inhibitors (SSRIs), previous studies have reported that SSRI medications do not treat fatigue and anergia symptoms well, and in some cases, can even worsen those symptoms. Subjects treated with dopamine (DA) uptake inhibitors, on the other hand, have been less likely to report symptoms of anergia and fatigue compared to those treated with SSRIs. Common DA uptake inhibitors such as methylphenidate and amphetamines, however, …

Characterization Of A Mycoplasma Pneumoniae Cards Toxin Mutant, Nikaash Pasnoori

Honors Scholar Theses

Mycoplasma pneumoniae is a high-burden pathogen which causes mild to significant infections of the respiratory system. According to the CDC, an estimated two million cases occur yearly in the United States alone, demonstrating the widespread effect of the pathogen. In addition to being the cause of respiratory infections, M. pneumoniae has also been implicated in exacerbating pre-existing asthma conditions. These morbidities make finding a vaccine candidate a vital part of easing the healthcare burden caused by the pathogen. The current mechanism of infection is unknown, but recent evidence points to the Community Acquired Respiratory Distress Syndrome (CARDS) toxin as being …

Amygdala And Neocortical Structural Volume Analysis In The Shank3b Mutant Mouse Model Of Autism Spectrum Disorder, Preet Sawhney

Honors Scholar Theses

Autism Spectrum Disorder (ASD) is a neurodevelopmental disorder that is characterized by abnormal social behavior, deficits in communication, and motor stereotypy. The SHANK3 gene, responsible for the generation of a scaffolding protein that is integral for the development of synapses, has been identified as one of the primary candidate genes implicated in the disorder. Shank3B is the rodent homolog for this gene. Research has shown that when this gene is disrupted in rodent models (e.g., via knock-out (KO)), ASD-like behaviors result. These include deficits in social interaction, increased anxiety, and repetitive self-grooming. The current study aimed to identify a physiological …

An Analysis Of Crispr-Cas Gene Editing In Agriculture, Ashley Laliberte

Honors Scholar Theses

The CRISPR-Cas system is a promising form of gene editing, especially for the agriculture industry. The ability to make single-nucleotide edits within a gene of interest, without the need to introduce foreign DNA, is a powerful tool for designing healthier and more efficient crops and food animals. This system provides opportunity for increased nutritional value, decreased food waste, and more economically and environmentally sustainable food production. Though this biotechnology is facing mechanistic limitations due to off-target effects and inefficient homology-directed repair, vast improvements have already been made to improve its efficacy. The CRISPR-Cas system is already the most advanced form …

Poor Maternal Nutrition During Gestation Alters Placental Igf-I, Igf-Ii, And Igfbp-3 Mrna Expression In Sheep, Caitlyn Splaine

Honors Scholar Theses

Insulin-like growth factors (IGF) modulate placental and fetal growth and development through nutrient sensing and endocrine signaling. We hypothesized that poor maternal nutrition during gestation would alter IGF-I, IGF binding protein (IGFBP)-2, and IGFBP-3 mRNA expression in the ovine placenta, but would not affect IGF-II mRNA expression. Pregnant ewes (n=57) were individually fed: 60% (restricted fed; RES), 100% (control fed; CON), or 140% (over fed; OVER) of National Research Council requirements for TDN starting at day 30±0.2 of gestation. Ewes were euthanized and cotyledon and caruncle samples were collected at days 45, 90, and 135 of gestation. Relative mRNA expression …

A Synergism Between Dimethyl Trisulfide And Methyl Thiolacetate In Attracting Carrion-Frequenting Beetles Demonstrated By Use Of A Chemically-Supplemented Minimal Trap, Stephen T. Trumbo, John Dicapua Iii

EEB Articles

Volatile organic compounds derived from microbes recruit insects to carrion, shaping community assembly and ecological succession. The importance of individual volatiles and interactions between volatiles are difficult to assess in the field because of (1) the myriad compounds from decomposing animals, and (2) the likelihood that complex component blends are important for the final approach to carrion. On the assumption that searching insects may use simpler volatile cues to orient at a distance, we employed a chemically-supplemented minimal trap that uses test chemicals to attract from a distance and a minimal carrion bait to induce trap entry. Traps supplemented with …

Using Harvest Slot Limits To Promote Stock Recovery And Broaden Age Structure In Marine Recreational Fisheries: A Case Study, Jacob M. Kasper, Jeffrey Brust, Amanda Caskenette, Jason Mcnamee, Jason C. Vokoun, Eric T. Schultz

EEB Articles

Fish populations with broad age distributions are expected to have higher reproductive capacity than age-truncated populations because of the disproportionate contributions of older fish. Harvest slot limits, an expected means of ameliorating age truncation, are modeled for Tautog Tautoga onitis in an overfished population subunit that is experiencing overfishing. Tautog, currently managed by a 40 cm minimum size limit (MSL), is a candidate species for slots because it is relatively long-lived, slow-growing, with low discard mortality. We evaluated changes in biomass and abundance-at-age relative to management with the current MSL regulations using a forward population simulation model for four slots: …